Chikungunya virus and its envelope protein E2 induce hyperalgesia in mice: Inhibition by anti-E2 monoclonal antibodies and by targeting TRPV1
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2023 |
| Outros Autores: | , , , , , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | por |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/57705 https://doi.org/10.3390/ cells12040556 |
Resumo: | Universidade Estadual de Londrina. Departamento Patologia. Laboratório de Dor, Inflamação, Neuropatia e Câncer. Londrina, PR, Brasil. |
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Segato-Vendrameto, Carina Z.Zanluca, CamilaZucoloto, Amanda Z.Zaninelli, Tiago H.Bertozzi, Mariana M.Saraiva-Santos, TelmaFerraz, Camila R.Staurengo-Ferrari, LarissaBadaro-Garcia, StephanieManchope, Marília F.Dionisio, Amanda M.Pinho-Ribeiro, Felipe A.Borghi, Sergio M.Mosimann, Ana Luiza PamplonaCasagrande, RubiaBordignon, JulianoFattori, VictorSantos, Claudia Nunes Duarte dos2023-04-06T19:20:39Z2023-04-06T19:20:39Z2023SEGATO-VENDRAMENTO, Carina Z. et al. Chikungunya virus and its envelope protein E2 induce hyperalgesia in mice: inhibition by anti-E2 monoclonal antibodies and by targeting TRPV1. Cells, v. 12, n. 556, p. 1–17, 2023.2073-4409https://www.arca.fiocruz.br/handle/icict/57705https://doi.org/10.3390/ cells12040556porMDPIChikungunya virus and its envelope protein E2 induce hyperalgesia in mice: Inhibition by anti-E2 monoclonal antibodies and by targeting TRPV1info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Estadual de Londrina. Departamento Patologia. Laboratório de Dor, Inflamação, Neuropatia e Câncer. Londrina, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.Universidade Estadual de Londrina. Departamento Patologia. Laboratório de Dor, Inflamação, Neuropatia e Câncer. Londrina, PR, Brasil.Universidade Estadual de Londrina. Departamento Patologia. Laboratório de Dor, Inflamação, Neuropatia e Câncer. Londrina, PR, Brasil.Universidade Estadual de Londrina. Departamento Patologia. Laboratório de Dor, Inflamação, Neuropatia e Câncer. Londrina, PR, Brasil.Universidade Estadual de Londrina. Departamento Patologia. Laboratório de Dor, Inflamação, Neuropatia e Câncer. Londrina, PR, Brasil.Universidade Estadual de Londrina. Departamento Patologia. Laboratório de Dor, Inflamação, Neuropatia e Câncer. Londrina, PR, Brasil.Universidade Estadual de Londrina. Departamento Patologia. Laboratório de Dor, Inflamação, Neuropatia e Câncer. Londrina, PR, Brasil.Universidade Estadual de Londrina. Departamento Patologia. Laboratório de Dor, Inflamação, Neuropatia e Câncer. Londrina, PR, Brasil.Universidade Estadual de Londrina. Departamento Patologia. Laboratório de Dor, Inflamação, Neuropatia e Câncer. Londrina, PR, Brasil.Universidade Estadual de Londrina. Departamento Patologia. Laboratório de Dor, Inflamação, Neuropatia e Câncer. Londrina, PR, Brasil.Universidade Estadual de Londrina. Departamento Patologia. Laboratório de Dor, Inflamação, Neuropatia e Câncer. Londrina, PR, Brasil. / Division of Dermatology. Department of Medicine. Washington University School of Medicine. St. Louis, USA.Universidade Estadual de Londrina. Departamento Patologia. Laboratório de Dor, Inflamação, Neuropatia e Câncer. Londrina, PR, Brasil. / Universidade Estadual do Norte do Paraná. Centro de Pesquisa em Ciências da Saúde. Londrina, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.Universidade Estadual de Londrina. Hospital Universitário (Centro de Ciências da Saúde). Departamento de Ciências Farmacêuticas. Londrina, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.Universidade Estadual de Londrina. Departamento Patologia. Laboratório de Dor, Inflamação, Neuropatia e Câncer. Londrina, PR, Brasil. / Vascular Biology Program. Boston Children’s Hospital. Harvard Medical School. Boston, USA.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.Universidade Estadual de Londrina. Departamento Patologia. Laboratório de Dor, Inflamação, Neuropatia e Câncer. Londrina, PR, Brasil.Chikungunya virus is an arthropod-borne infectious agent that causes Chikungunya fever disease. About 90% of the infected patients experience intense polyarthralgia, affecting mainly the extremities but also the large joints such as the knees. Chronic disease symptoms persist for months, even after clearance of the virus from the blood. Envelope proteins stimulate the immune response against the Chikungunya virus, becoming an important therapeutic target. We inactivated the Chikungunya virus (iCHIKV) and produced recombinant E2 (rE2) protein and three different types of anti-rE2 monoclonal antibodies. Using these tools, we observed that iCHIKV and rE2 protein induced mechanical hyperalgesia (electronic aesthesiometer test) and thermal hyperalgesia (Hargreaves test) in mice. These behavioral results were accompanied by the activation of dorsal root ganglia (DRG) neurons in mice, as observed by calcium influx. Treatment with three different types of anti-rE2 monoclonal antibodies and absence or blockade (AMG-9810 treatment) of transient receptor potential vanilloid 1 (TRPV1) channel diminished mechanical and thermal hyperalgesia in mice. iCHIKV and rE2 activated TRPV1+ mouse DRG neurons in vitro, demonstrating their ability to activate nociceptor sensory neurons directly. Therefore, our mouse data demonstrate that targeting E2 CHIKV protein with monoclonal antibodies and inhibiting TRPV1 channels are reasonable strategies to control CHIKV pain.Chikungunya virusViral Envelope ProteinsAntibodies, MonoclonalJoint PainTRPV Cation ChannelsProteínas del Envoltorio ViralAnticuerpos MonoclonalesProtéines de l'enveloppe viraleAnticorps monoclonauxArthralgieVírus ChikungunyaProteínas do Envelope Viral E2Anticorpos MonoclonaisPoliartralgiaReceptores Vaniloidesinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/57705/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALcells-12-00556OK.pdfcells-12-00556OK.pdfapplication/pdf4471956https://www.arca.fiocruz.br/bitstream/icict/57705/2/cells-12-00556OK.pdf5aac40f0f5c08211764d77643df71b39MD52icict/577052023-04-06 16:20:40.631oai:www.arca.fiocruz.br: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ório 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| dc.title.en_US.fl_str_mv |
Chikungunya virus and its envelope protein E2 induce hyperalgesia in mice: Inhibition by anti-E2 monoclonal antibodies and by targeting TRPV1 |
| title |
Chikungunya virus and its envelope protein E2 induce hyperalgesia in mice: Inhibition by anti-E2 monoclonal antibodies and by targeting TRPV1 |
| spellingShingle |
Chikungunya virus and its envelope protein E2 induce hyperalgesia in mice: Inhibition by anti-E2 monoclonal antibodies and by targeting TRPV1 Segato-Vendrameto, Carina Z. Chikungunya virus Viral Envelope Proteins Antibodies, Monoclonal Joint Pain TRPV Cation Channels Proteínas del Envoltorio Viral Anticuerpos Monoclonales Protéines de l'enveloppe virale Anticorps monoclonaux Arthralgie Vírus Chikungunya Proteínas do Envelope Viral E2 Anticorpos Monoclonais Poliartralgia Receptores Vaniloides |
| title_short |
Chikungunya virus and its envelope protein E2 induce hyperalgesia in mice: Inhibition by anti-E2 monoclonal antibodies and by targeting TRPV1 |
| title_full |
Chikungunya virus and its envelope protein E2 induce hyperalgesia in mice: Inhibition by anti-E2 monoclonal antibodies and by targeting TRPV1 |
| title_fullStr |
Chikungunya virus and its envelope protein E2 induce hyperalgesia in mice: Inhibition by anti-E2 monoclonal antibodies and by targeting TRPV1 |
| title_full_unstemmed |
Chikungunya virus and its envelope protein E2 induce hyperalgesia in mice: Inhibition by anti-E2 monoclonal antibodies and by targeting TRPV1 |
| title_sort |
Chikungunya virus and its envelope protein E2 induce hyperalgesia in mice: Inhibition by anti-E2 monoclonal antibodies and by targeting TRPV1 |
| author |
Segato-Vendrameto, Carina Z. |
| author_facet |
Segato-Vendrameto, Carina Z. Zanluca, Camila Zucoloto, Amanda Z. Zaninelli, Tiago H. Bertozzi, Mariana M. Saraiva-Santos, Telma Ferraz, Camila R. Staurengo-Ferrari, Larissa Badaro-Garcia, Stephanie Manchope, Marília F. Dionisio, Amanda M. Pinho-Ribeiro, Felipe A. Borghi, Sergio M. Mosimann, Ana Luiza Pamplona Casagrande, Rubia Bordignon, Juliano Fattori, Victor Santos, Claudia Nunes Duarte dos |
| author_role |
author |
| author2 |
Zanluca, Camila Zucoloto, Amanda Z. Zaninelli, Tiago H. Bertozzi, Mariana M. Saraiva-Santos, Telma Ferraz, Camila R. Staurengo-Ferrari, Larissa Badaro-Garcia, Stephanie Manchope, Marília F. Dionisio, Amanda M. Pinho-Ribeiro, Felipe A. Borghi, Sergio M. Mosimann, Ana Luiza Pamplona Casagrande, Rubia Bordignon, Juliano Fattori, Victor Santos, Claudia Nunes Duarte dos |
| author2_role |
author author author author author author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Segato-Vendrameto, Carina Z. Zanluca, Camila Zucoloto, Amanda Z. Zaninelli, Tiago H. Bertozzi, Mariana M. Saraiva-Santos, Telma Ferraz, Camila R. Staurengo-Ferrari, Larissa Badaro-Garcia, Stephanie Manchope, Marília F. Dionisio, Amanda M. Pinho-Ribeiro, Felipe A. Borghi, Sergio M. Mosimann, Ana Luiza Pamplona Casagrande, Rubia Bordignon, Juliano Fattori, Victor Santos, Claudia Nunes Duarte dos |
| dc.subject.en.en_US.fl_str_mv |
Chikungunya virus Viral Envelope Proteins Antibodies, Monoclonal Joint Pain TRPV Cation Channels |
| topic |
Chikungunya virus Viral Envelope Proteins Antibodies, Monoclonal Joint Pain TRPV Cation Channels Proteínas del Envoltorio Viral Anticuerpos Monoclonales Protéines de l'enveloppe virale Anticorps monoclonaux Arthralgie Vírus Chikungunya Proteínas do Envelope Viral E2 Anticorpos Monoclonais Poliartralgia Receptores Vaniloides |
| dc.subject.es.en_US.fl_str_mv |
Proteínas del Envoltorio Viral Anticuerpos Monoclonales |
| dc.subject.fr.en_US.fl_str_mv |
Protéines de l'enveloppe virale Anticorps monoclonaux Arthralgie |
| dc.subject.decs.en_US.fl_str_mv |
Vírus Chikungunya Proteínas do Envelope Viral E2 Anticorpos Monoclonais Poliartralgia Receptores Vaniloides |
| description |
Universidade Estadual de Londrina. Departamento Patologia. Laboratório de Dor, Inflamação, Neuropatia e Câncer. Londrina, PR, Brasil. |
| publishDate |
2023 |
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2023-04-06T19:20:39Z |
| dc.date.available.fl_str_mv |
2023-04-06T19:20:39Z |
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2023 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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SEGATO-VENDRAMENTO, Carina Z. et al. Chikungunya virus and its envelope protein E2 induce hyperalgesia in mice: inhibition by anti-E2 monoclonal antibodies and by targeting TRPV1. Cells, v. 12, n. 556, p. 1–17, 2023. |
| dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/57705 |
| dc.identifier.issn.en_US.fl_str_mv |
2073-4409 |
| dc.identifier.doi.none.fl_str_mv |
https://doi.org/10.3390/ cells12040556 |
| identifier_str_mv |
SEGATO-VENDRAMENTO, Carina Z. et al. Chikungunya virus and its envelope protein E2 induce hyperalgesia in mice: inhibition by anti-E2 monoclonal antibodies and by targeting TRPV1. Cells, v. 12, n. 556, p. 1–17, 2023. 2073-4409 |
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https://www.arca.fiocruz.br/handle/icict/57705 https://doi.org/10.3390/ cells12040556 |
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por |
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MDPI |
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MDPI |
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