In-depth phylogenetic analysis of hepatitis C virus subtype 1a and occurrence of 80K and associated polymorphisms in the NS3 protease

Detalhes bibliográficos
Autor(a) principal: Santos, André F.
Data de Publicação: 2016
Outros Autores: Bello, Gonzalo, Vidal, Luãnna L., Souza, Suiane L., Mir, Daiana, Soares, Marcelo A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/17950
Resumo: Universidade Federal do Rio de Janeiro. Departamento de Genética. Rio de Janeiro, RJ, Brasil.
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spelling Santos, André F.Bello, GonzaloVidal, Luãnna L.Souza, Suiane L.Mir, DaianaSoares, Marcelo A.2017-03-06T19:11:50Z2017-03-06T19:11:50Z2016SANTOS, André F. et al. In-depth phylogenetic analysis of hepatitis C virus subtype 1a and occurrence of 80K and associated´polymorphisms in the NS3 protease. Scientific Reports, v.6:31780, 6p, Aug. 2016.2045-2322https://www.arca.fiocruz.br/handle/icict/1795010.1038/srep31780engNature Publishing GroupHepatite C subtipo1aAnálise filogenéticaPolimorfismoshepatitis C virus subtype 1aphylogenetic analysisNS3 proteasepolymorphismsIn-depth phylogenetic analysis of hepatitis C virus subtype 1a and occurrence of 80K and associated polymorphisms in the NS3 proteaseinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Rio de Janeiro. Departamento de Genética. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de AIDS e Imunologia Molecular. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Departamento de Genética. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Departamento de Genética. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de AIDS e Imunologia Molecular. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Departamento de Genética. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Câncer. Programa de Oncovirologia. Rio de Janeiro, RJ, Brasil.HCV genetic diversity is high and impacts disease progression, treatment and drug resistance. HCV subtype 1a is divided in two clades (I and II), and the 80 K natural polymorphism in the viral NS3 protease is prevalent in clade I. Paradoxically, countries dominated by this clade have contrasting frequencies of 80 K. Over 2,000 HCV 1a NS3 sequences were retrieved from public databases representing Europe, Oceania and the Americas. Sequences were aligned with HCV reference sequences and subjected to phylogenetic analysis to investigate the relative presence of different subtype 1a clades and NS3 protease mutations. HCV-1a sequences split into clades I and II. Clade I was further structured into three subclades, IA to C. Sub-clade IA prevailed in the U.S., while subclade IC was major in Brazil. The NS3 80 K polymorphism was associated with subclade IA, but nearly absent in subclades IB and IC, a pattern similarly seen for the 91S/T compensatory mutation. Three HCV-1a-I sub-clades have been identified, with different frequencies in distinct regions. The 80 K and 91A/S mutations were associated with subclade IA, which provide an explanation for the disparities seen in simeprevir resistance profiles of countries dominated by HCV 1a-I, like the U.S. and Brazil.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/17950/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALGonzalo3_bello_etal_IOC_2016.´df.pdfGonzalo3_bello_etal_IOC_2016.´df.pdfapplication/pdf1203851https://www.arca.fiocruz.br/bitstream/icict/17950/2/Gonzalo3_bello_etal_IOC_2016.%c2%b4df.pdfbb3f263a3e8e629e01099978e23fb567MD52TEXTGonzalo3_bello_etal_IOC_2016.´df.pdf.txtGonzalo3_bello_etal_IOC_2016.´df.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv In-depth phylogenetic analysis of hepatitis C virus subtype 1a and occurrence of 80K and associated polymorphisms in the NS3 protease
title In-depth phylogenetic analysis of hepatitis C virus subtype 1a and occurrence of 80K and associated polymorphisms in the NS3 protease
spellingShingle In-depth phylogenetic analysis of hepatitis C virus subtype 1a and occurrence of 80K and associated polymorphisms in the NS3 protease
Santos, André F.
Hepatite C subtipo1a
Análise filogenética
Polimorfismos
hepatitis C virus subtype 1a
phylogenetic analysis
NS3 protease
polymorphisms
title_short In-depth phylogenetic analysis of hepatitis C virus subtype 1a and occurrence of 80K and associated polymorphisms in the NS3 protease
title_full In-depth phylogenetic analysis of hepatitis C virus subtype 1a and occurrence of 80K and associated polymorphisms in the NS3 protease
title_fullStr In-depth phylogenetic analysis of hepatitis C virus subtype 1a and occurrence of 80K and associated polymorphisms in the NS3 protease
title_full_unstemmed In-depth phylogenetic analysis of hepatitis C virus subtype 1a and occurrence of 80K and associated polymorphisms in the NS3 protease
title_sort In-depth phylogenetic analysis of hepatitis C virus subtype 1a and occurrence of 80K and associated polymorphisms in the NS3 protease
author Santos, André F.
author_facet Santos, André F.
Bello, Gonzalo
Vidal, Luãnna L.
Souza, Suiane L.
Mir, Daiana
Soares, Marcelo A.
author_role author
author2 Bello, Gonzalo
Vidal, Luãnna L.
Souza, Suiane L.
Mir, Daiana
Soares, Marcelo A.
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Santos, André F.
Bello, Gonzalo
Vidal, Luãnna L.
Souza, Suiane L.
Mir, Daiana
Soares, Marcelo A.
dc.subject.other.pt_BR.fl_str_mv Hepatite C subtipo1a
Análise filogenética
Polimorfismos
topic Hepatite C subtipo1a
Análise filogenética
Polimorfismos
hepatitis C virus subtype 1a
phylogenetic analysis
NS3 protease
polymorphisms
dc.subject.en.pt_BR.fl_str_mv hepatitis C virus subtype 1a
phylogenetic analysis
NS3 protease
polymorphisms
description Universidade Federal do Rio de Janeiro. Departamento de Genética. Rio de Janeiro, RJ, Brasil.
publishDate 2016
dc.date.issued.fl_str_mv 2016
dc.date.accessioned.fl_str_mv 2017-03-06T19:11:50Z
dc.date.available.fl_str_mv 2017-03-06T19:11:50Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SANTOS, André F. et al. In-depth phylogenetic analysis of hepatitis C virus subtype 1a and occurrence of 80K and associated´polymorphisms in the NS3 protease. Scientific Reports, v.6:31780, 6p, Aug. 2016.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/17950
dc.identifier.issn.pt_BR.fl_str_mv 2045-2322
dc.identifier.eissn.none.fl_str_mv 10.1038/srep31780
identifier_str_mv SANTOS, André F. et al. In-depth phylogenetic analysis of hepatitis C virus subtype 1a and occurrence of 80K and associated´polymorphisms in the NS3 protease. Scientific Reports, v.6:31780, 6p, Aug. 2016.
2045-2322
10.1038/srep31780
url https://www.arca.fiocruz.br/handle/icict/17950
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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