Schistosoma mansoni: a method for inducing resistance to praziquantel using infected Biompharia glabrata snails.

Detalhes bibliográficos
Autor(a) principal: Couto, Flávia Fernanda Bubula
Data de Publicação: 2011
Outros Autores: Coelho, Paulo Marcos Zech, Pereira, Neusa Araújo, Kusel, John Robert, Katz, Naftale, Passos, Liana konovaloff Jannotti, Mattos, Ana Carolina Alves de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: http://dx.doi.org/10.1590/S0074-02762011000200006
https://www.arca.fiocruz.br/handle/icict/8643
Resumo: Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Esquistossomose. Belo Horizonte, MG, Brasil
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spelling Couto, Flávia Fernanda BubulaCoelho, Paulo Marcos ZechPereira, Neusa AraújoKusel, John RobertKatz, NaftalePassos, Liana konovaloff JannottiMattos, Ana Carolina Alves de2014-10-21T16:48:16Z2014-10-21T16:48:16Z2011COUTO, Flávia Fernanda Bubula et al. Schistosoma mansoni: a method for inducing resistance to praziquantel using infected Biompharia glabrata snails. Memorias do Instituto Oswaldo Cruz, 106(2): 153-157, 2011.0074-0276http://dx.doi.org/10.1590/S0074-02762011000200006https://www.arca.fiocruz.br/handle/icict/8643engFundação Oswaldo CruzSchistosoma mansoni: a method for inducing resistance to praziquantel using infected Biompharia glabrata snails.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Esquistossomose. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Esquistossomose. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Esquistossomose. Belo Horizonte, MG, BrasilUniversity of Glasgow Faculty of Biomedical and Life Sciences. Division of Infection and Immunity. Scotland, UKFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Esquistossomose. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Moluscário Lobato Paraense. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Esquistossomose. Belo Horizonte, MG, BrasilTo elucidate the mechanisms of antischistosoma resistance, drug-resistant Schistosoma mansoni laboratory isolates are essential. We developed a new method for inducing resistance to praziquantel (PZQ) using successive drug treatments of Biomphalaria glabrata snails infected with S. mansoni. Infected B. glabrata were treated three times with 100 mg/kg PZQ for five consecutive days with a one-week interval between them. After the treatment, the cercariae (LE-PZQ) produced from these snails and the LE strains (susceptible) were used to infect mice. Forty-five days after infection, mice were treated with 200, 400 or 800 mg/kg PZQ. Thirty days post-treatment, we observed that the mean number of worms recovered by perfusion was significantly higher in the group of mice infected with the LE-PZQ isolate treated with 200 and 400 mg/kg in comparison to the LE strain with the same treatment. Moreover, there was a significant difference between the ED50 (effective dose required to kill 50% of the worms) of the LE-PZQ isolate (362 mg/kg) and the LE strain (68 mg/kg). In the in vitro assays, the worms of the LE-PZQ isolate were also less susceptible to PZQ. Thus, the use of infected snails as an experimental model for development of resistance to S. mansoni is effective, fast, simple and cheap.Schistosoma mansoniBiomphalaria glabratapraziquantelresistance induction/new methodinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/8643/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALSchistosoma mansoni.pdfSchistosoma mansoni.pdfapplication/pdf374987https://www.arca.fiocruz.br/bitstream/icict/8643/2/Schistosoma%20mansoni.pdf785dbf9c872948e3f7940c705071b0c6MD52TEXTSchistosoma mansoni.pdf.txtSchistosoma mansoni.pdf.txtExtracted texttext/plain26988https://www.arca.fiocruz.br/bitstream/icict/8643/3/Schistosoma%20mansoni.pdf.txtb71243212b9d2156483fec0d25f0e1ceMD53icict/86432019-06-19 10:11:34.064oai:www.arca.fiocruz.br:icict/8643TElDRU7Dh0EgREUgRElTVFJJQlVJw4fDg08gTsODTy1FWENMVVNJVkEKCkFvIGNvbmNvcmRhciBlIGFjZWl0YXIgZXN0YSBsaWNlbsOnYSB2b2PDqiAoYXV0b3Igb3UgZGV0ZW50b3IgZG9zIGRpcmVpdG9zIGF1dG9yYWlzKToKCmEpIERlY2xhcmEgcXVlIGNvbmhlY2UgYSBwb2zDrXRpY2EgZGUgY29weXJpZ2h0IGRhIGVkaXRvcmEgZG8gc2V1IGRvY3VtZW50by4KCmIpIERlY2xhcmEgcXVlIGNvbmhlY2UgZSBhY2VpdGEgYXMgRGlyZXRyaXplcyBwYXJhIG8gUmVwb3NpdMOzcmlvIEluc3RpdHVjaW9uYWwgZGEgRnVuZGHDp8OjbyBPc3dhbGRvIENydXogKEZJT0NSVVopLgoKYykgQ29uY2VkZSDDoCBGSU9DUlVaIG8gZGlyZWl0byBuw6NvLWV4Y2x1c2l2byBkZSBhcnF1aXZhciwgcmVwcm9kdXppciwgY29udmVydGVyIChjb21vIGRlZmluaWRvIGEgc2VndWlyKSwgY29tdW5pY2FyCiAKZS9vdSBkaXN0cmlidWlyIG5vIFJlcG9zaXTDs3JpbyBkYSBGSU9DUlVaLCBvIGRvY3VtZW50byBlbnRyZWd1ZSAoaW5jbHVpbmRvIG8gcmVzdW1vL2Fic3RyYWN0KSBlbSBmb3JtYXRvIGRpZ2l0YWwgb3UgCgpwb3IgcXVhbHF1ZXIgb3V0cm8gbWVpby4KCmQpIERlY2xhcmEgcXVlIGF1dG9yaXphIGEgRklPQ1JVWiBhIGFycXVpdmFyIG1haXMgZGUgdW1hIGPDs3BpYSBkZXN0ZSBkb2N1bWVudG8gZSBjb252ZXJ0w6otbG8sIHNlbSBhbHRlcmFyIG8gc2V1IGNvbnRlw7pkbywgCgpwYXJhIHF1YWxxdWVyIGZvcm1hdG8gZGUgYXJxdWl2bywgbWVpbyBvdSBzdXBvcnRlLCBwYXJhIGVmZWl0b3MgZGUgc2VndXJhbsOnYSwgcHJlc2VydmHDp8OjbyAoYmFja3VwKSBlIGFjZXNzby4KCmUpIERlY2xhcmEgcXVlIG8gZG9jdW1lbnRvIHN1Ym1ldGlkbyDDqSBvIHNldSB0cmFiYWxobyBvcmlnaW5hbCwgZSBxdWUgZGV0w6ltIG8gZGlyZWl0byBkZSBjb25jZWRlciBhIHRlcmNlaXJvcyBvcyBkaXJlaXRvcyAKCmNvbnRpZG9zIG5lc3RhIGxpY2Vuw6dhLiBEZWNsYXJhIHRhbWLDqW0gcXVlIGEgZW50cmVnYSBkbyBkb2N1bWVudG8gbsOjbyBpbmZyaW5nZSBvcyBkaXJlaXRvcyBkZSBxdWFscXVlciBvdXRyYSBwZXNzb2Egb3UgZW50aWRhZGUuCgpmKSBEZWNsYXJhIHF1ZSwgbm8gY2FzbyBkbyBkb2N1bWVudG8gc3VibWV0aWRvIGNvbnRlciBtYXRlcmlhbCBkbyBxdWFsIG7Do28gZGV0w6ltIG9zIGRpcmVpdG9zIGRlIGF1dG9yLCBvYnRldmUgYSBhdXRvcml6YcOnw6NvIAoKaXJyZXN0cml0YSBkbyByZXNwZWN0aXZvIGRldGVudG9yIGRlc3NlcyBkaXJlaXRvcywgcGFyYSBjZWRlciBhIEZJT0NSVVogb3MgZGlyZWl0b3MgcmVxdWVyaWRvcyBwb3IgZXN0YSBMaWNlbsOnYSBlIGF1dG9yaXphciBhIAoKdXRpbGl6w6EtbG9zIGxlZ2FsbWVudGUuIERlY2xhcmEgdGFtYsOpbSBxdWUgZXNzZSBtYXRlcmlhbCBjdWpvcyBkaXJlaXRvcyBzw6NvIGRlIHRlcmNlaXJvcyBlc3TDoSBjbGFyYW1lbnRlIGlkZW50aWZpY2FkbyBlIHJlY29uaGVjaWRvIAoKbm8gdGV4dG8gb3UgY29udGXDumRvIGRvIGRvY3VtZW50byBlbnRyZWd1ZS4KCmcpIFNFIE8gRE9DVU1FTlRPIEVOVFJFR1VFIMOJIEJBU0VBRE8gRU0gVFJBQkFMSE8gRklOQU5DSUFETyBPVSBBUE9JQURPIFBPUiBPVVRSQSBJTlNUSVRVScOHw4NPIFFVRSBOw4NPIEEgRklPQ1JVWiwgREVDTEFSQSBRVUUgQ1VNUFJJVSAKClFVQUlTUVVFUiBPQlJJR0HDh8OVRVMgRVhJR0lEQVMgUEVMTyBSRVNQRUNUSVZPIENPTlRSQVRPIE9VIEFDT1JETy4gQSBGSU9DUlVaIGlkZW50aWZpY2Fyw6EgY2xhcmFtZW50ZSBvKHMpIG5vbWUocykgZG8ocykgYXV0b3IoZXMpIGRvcyAKCmRpcmVpdG9zIGRvIGRvY3VtZW50byBlbnRyZWd1ZSBlIG7Do28gZmFyw6EgcXVhbHF1ZXIgYWx0ZXJhw6fDo28sIHBhcmEgYWzDqW0gZG8gcHJldmlzdG8gbmEgYWzDrW5lYSBjKS4KRepositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352019-06-19T13:11:34Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Schistosoma mansoni: a method for inducing resistance to praziquantel using infected Biompharia glabrata snails.
title Schistosoma mansoni: a method for inducing resistance to praziquantel using infected Biompharia glabrata snails.
spellingShingle Schistosoma mansoni: a method for inducing resistance to praziquantel using infected Biompharia glabrata snails.
Couto, Flávia Fernanda Bubula
Schistosoma mansoni
Biomphalaria glabrata
praziquantel
resistance induction/new method
title_short Schistosoma mansoni: a method for inducing resistance to praziquantel using infected Biompharia glabrata snails.
title_full Schistosoma mansoni: a method for inducing resistance to praziquantel using infected Biompharia glabrata snails.
title_fullStr Schistosoma mansoni: a method for inducing resistance to praziquantel using infected Biompharia glabrata snails.
title_full_unstemmed Schistosoma mansoni: a method for inducing resistance to praziquantel using infected Biompharia glabrata snails.
title_sort Schistosoma mansoni: a method for inducing resistance to praziquantel using infected Biompharia glabrata snails.
author Couto, Flávia Fernanda Bubula
author_facet Couto, Flávia Fernanda Bubula
Coelho, Paulo Marcos Zech
Pereira, Neusa Araújo
Kusel, John Robert
Katz, Naftale
Passos, Liana konovaloff Jannotti
Mattos, Ana Carolina Alves de
author_role author
author2 Coelho, Paulo Marcos Zech
Pereira, Neusa Araújo
Kusel, John Robert
Katz, Naftale
Passos, Liana konovaloff Jannotti
Mattos, Ana Carolina Alves de
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Couto, Flávia Fernanda Bubula
Coelho, Paulo Marcos Zech
Pereira, Neusa Araújo
Kusel, John Robert
Katz, Naftale
Passos, Liana konovaloff Jannotti
Mattos, Ana Carolina Alves de
dc.subject.en.pt_BR.fl_str_mv Schistosoma mansoni
Biomphalaria glabrata
praziquantel
resistance induction/new method
topic Schistosoma mansoni
Biomphalaria glabrata
praziquantel
resistance induction/new method
description Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Esquistossomose. Belo Horizonte, MG, Brasil
publishDate 2011
dc.date.issued.fl_str_mv 2011
dc.date.accessioned.fl_str_mv 2014-10-21T16:48:16Z
dc.date.available.fl_str_mv 2014-10-21T16:48:16Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv COUTO, Flávia Fernanda Bubula et al. Schistosoma mansoni: a method for inducing resistance to praziquantel using infected Biompharia glabrata snails. Memorias do Instituto Oswaldo Cruz, 106(2): 153-157, 2011.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/8643
dc.identifier.issn.none.fl_str_mv 0074-0276
http://dx.doi.org/10.1590/S0074-02762011000200006
identifier_str_mv COUTO, Flávia Fernanda Bubula et al. Schistosoma mansoni: a method for inducing resistance to praziquantel using infected Biompharia glabrata snails. Memorias do Instituto Oswaldo Cruz, 106(2): 153-157, 2011.
0074-0276
url http://dx.doi.org/10.1590/S0074-02762011000200006
https://www.arca.fiocruz.br/handle/icict/8643
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz
publisher.none.fl_str_mv Fundação Oswaldo Cruz
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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