Substrate specificity of the Trypanosoma cruzi trans-sialidase

Detalhes bibliográficos
Autor(a) principal: Vandekerckhove, Filip
Data de Publicação: 1992
Outros Autores: Schenkman, Sergio, Pontes-de-Carvalho, Lain Carlos, Tomlinson, Stephen, Kiso, Makoto, Yoshida, Masahiro, Hasegawa, Akira, Nussenzweig, Victor
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/32241
Resumo: Pontes-de-Carvalho, Lain Carlos. Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. “Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento”.
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spelling Vandekerckhove, FilipSchenkman, SergioPontes-de-Carvalho, Lain CarlosTomlinson, StephenKiso, MakotoYoshida, MasahiroHasegawa, AkiraNussenzweig, Victor2019-03-27T12:56:13Z2019-03-27T12:56:13Z1992VANDEKERCKHOVE, F. et al. Substrate specificity of the Trypanosoma cruzi trans-sialidase. Glycobiology, v. 2, n. 6, p. 541-548, 1992.0959-6658https://www.arca.fiocruz.br/handle/icict/32241Pontes-de-Carvalho, Lain Carlos. Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. “Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento”.MacArthur Foundation, the UNDP/World Bank/WHO Special Program for Research and Training in Tropical Diseases, Conselho Nacional de Desenvolvimento Cientifico e Tecnologico and Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (Brazil) F.V. is a Research Assistant of the Belgian National Fund for Scientific Research (NFWO), L.P.C is a recipient of a grant from the Secretana de Ciencia e Tecnologia (Programa RHAE), Brazil.New York University Medical Center. Department of Pathology. Division of Immunology. New York, NY, USA.Escola Paulista de Medicina. Disciplina de Biologia Celular. São Paulo, SP, Brasil.Escola Paulista de Medicina. Disciplina de Biologia Celular. São Paulo, SP, Brasil.Escola Paulista de Medicina. Disciplina de Biologia Celular. São Paulo, SP, Brasil.Gifu University. Department of Applied Bioorganic Chemistry. Gifu, Japan.Gifu University. Department of Applied Bioorganic Chemistry. Gifu, Japan.Gifu University. Department of Applied Bioorganic Chemistry. Gifu, Japan.Escola Paulista de Medicina. Disciplina de Biologia Celular. São Paulo, SP, Brasil.Trypanosoma cruzi trypomastigotes acquire sialic acid (SA) from host glycoconjugates by means of a plasma membraneassociated trans-sialidase (TS). Here we study the substrate specificity of TS, which differs from all known sialyltransferases in that it does not require cytidine monophosphate (CMD-SA as donor. The T.cruzi TS reversibly transfers SA to saccharides with terminal /3-Gal (but not a-Gal) residues. Donors are saccharides with SA linked to terminal /3-Gal residues by (a2-3), but not (a2-6) bonds. The type of /3-linkage of the terminal Gal residue is of minor importance (/31-4 and 01-6 are slightly better than /Sl-3), whereas chain length and the structure of additional vicinal sugar residues are not relevant. SA on the surface of living trypomastigotes of T. cruzi is transferred back and forth between the parasite surface and acceptor molecules with terminal /3-Gal, either in solution or on the surface of neighbouring mammalian cells. Addition of fucose residue on or close to the terminal galactose impairs TS activity. As a consequence, the enzyme acts poorly on the E-selectin ligand sialyl-Lewis" and its precursor Lewis1, and in vitro adhesion of TS-treated neutrophils to L-cells expressing L-selectin is not affected. Modifications in the structure of the (a2-3)-linked iV-acetyl-neuraminic acid (Neu5Ac) (deoxy or methoxv) of the donor molecules do not impair transfer if the changes are at C9, whereas changes at C4, C7 and C8 impair the ability to donate the modified SA. Compounds with modified C4 and C8 inhibit TS at relatively high inhibitor/substrate ratios.engOxford University PressSelectinasÁcido siálicoSialidaseTrans-sialidaseTrypanosoma cruziSelectinsSialic acidSialidaseTrans-sialidaseTrypanosoma cruziSubstrate specificity of the Trypanosoma cruzi trans-sialidaseinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/32241/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALVandekerckhove F Substrate specificity 1992.pdfVandekerckhove F Substrate specificity 1992.pdfapplication/pdf3069261https://www.arca.fiocruz.br/bitstream/icict/32241/2/Vandekerckhove%20F%20Substrate%20specificity%201992.pdf19a803f5c093f43437c880ae11e252a1MD52TEXTVandekerckhove F Substrate specificity 1992.pdf.txtVandekerckhove F Substrate specificity 1992.pdf.txtExtracted texttext/plain48344https://www.arca.fiocruz.br/bitstream/icict/32241/3/Vandekerckhove%20F%20Substrate%20specificity%201992.pdf.txtc13c0392c8a7a04cc96a69bd20428ffdMD53icict/322412023-03-15 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dc.title.pt_BR.fl_str_mv Substrate specificity of the Trypanosoma cruzi trans-sialidase
title Substrate specificity of the Trypanosoma cruzi trans-sialidase
spellingShingle Substrate specificity of the Trypanosoma cruzi trans-sialidase
Vandekerckhove, Filip
Selectinas
Ácido siálico
Sialidase
Trans-sialidase
Trypanosoma cruzi
Selectins
Sialic acid
Sialidase
Trans-sialidase
Trypanosoma cruzi
title_short Substrate specificity of the Trypanosoma cruzi trans-sialidase
title_full Substrate specificity of the Trypanosoma cruzi trans-sialidase
title_fullStr Substrate specificity of the Trypanosoma cruzi trans-sialidase
title_full_unstemmed Substrate specificity of the Trypanosoma cruzi trans-sialidase
title_sort Substrate specificity of the Trypanosoma cruzi trans-sialidase
author Vandekerckhove, Filip
author_facet Vandekerckhove, Filip
Schenkman, Sergio
Pontes-de-Carvalho, Lain Carlos
Tomlinson, Stephen
Kiso, Makoto
Yoshida, Masahiro
Hasegawa, Akira
Nussenzweig, Victor
author_role author
author2 Schenkman, Sergio
Pontes-de-Carvalho, Lain Carlos
Tomlinson, Stephen
Kiso, Makoto
Yoshida, Masahiro
Hasegawa, Akira
Nussenzweig, Victor
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Vandekerckhove, Filip
Schenkman, Sergio
Pontes-de-Carvalho, Lain Carlos
Tomlinson, Stephen
Kiso, Makoto
Yoshida, Masahiro
Hasegawa, Akira
Nussenzweig, Victor
dc.subject.other.pt_BR.fl_str_mv Selectinas
Ácido siálico
Sialidase
Trans-sialidase
Trypanosoma cruzi
topic Selectinas
Ácido siálico
Sialidase
Trans-sialidase
Trypanosoma cruzi
Selectins
Sialic acid
Sialidase
Trans-sialidase
Trypanosoma cruzi
dc.subject.en.pt_BR.fl_str_mv Selectins
Sialic acid
Sialidase
Trans-sialidase
Trypanosoma cruzi
description Pontes-de-Carvalho, Lain Carlos. Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. “Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento”.
publishDate 1992
dc.date.issued.fl_str_mv 1992
dc.date.accessioned.fl_str_mv 2019-03-27T12:56:13Z
dc.date.available.fl_str_mv 2019-03-27T12:56:13Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv VANDEKERCKHOVE, F. et al. Substrate specificity of the Trypanosoma cruzi trans-sialidase. Glycobiology, v. 2, n. 6, p. 541-548, 1992.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/32241
dc.identifier.issn.pt_BR.fl_str_mv 0959-6658
identifier_str_mv VANDEKERCKHOVE, F. et al. Substrate specificity of the Trypanosoma cruzi trans-sialidase. Glycobiology, v. 2, n. 6, p. 541-548, 1992.
0959-6658
url https://www.arca.fiocruz.br/handle/icict/32241
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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https://www.arca.fiocruz.br/bitstream/icict/32241/3/Vandekerckhove%20F%20Substrate%20specificity%201992.pdf.txt
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