Tolerogenic Dendritic Cells Reduce Airway Inflammation in a Model of Dust Mite Triggered Allergic Inflammation
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2018 |
| Outros Autores: | , , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/28055 |
Resumo: | Programa de Excelência em Pesquisa (PROEP-CNPq). |
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França, Luciana Souza de AragãoRocha, Viviane Costa JunqueiraAndrade, André CronembergerCosta, F H BVasconcelos, José FernandesAthanazio, Daniel AbensurSilva, Daniela NascimentoSantos, Emanuelle de SouzaMeira, Cássio SantanaAraújo, Cintia Figueiredo deCerqueira, Jéssica VieiraCardillo, FabíolaNeves, Neuza Maria AlcântaraSoares, Milena Botelho PereiraPontes-de-Carvalho, Lain Carlos2018-08-10T13:45:55Z2018-08-10T13:45:55Z2018FRANÇA, L. S. A. et al. Tolerogenic Dendritic Cells Reduce Airway Inflammation in a Model of Dust Mite Triggered Allergic Inflammation. Allergy Asthma and Immunology Research, v. 10, n. 4, p. 406-419, 2018.2092-7355https://www.arca.fiocruz.br/handle/icict/2805510.4168/aair.2018.10.4.406Programa de Excelência em Pesquisa (PROEP-CNPq).Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Hospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, BrasilFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, BrasilUniversidade de São Paulo. Departamento de Medicina. São Paulo, SP, BrasilUniversity of Texas Health Science Center. Department of Diagnostics and Biomedical Sciences. Houston, USAFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Hospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, BrasilFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina. Salvador, BA, BrasilHospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, BrasilFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, BrasilUniversidade Federal da Bahia. Hospital Universitário Edgard Santos. Serviço de Imunologia. Salvador, BA, BrasilFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, BrasilUniversidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, BrasilFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Hospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, BrasilFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, BrasilThe use of tolerogenic dendritic cells (TolDCs) to control exacerbated immune responses may be a prophylactic and therapeutic option for application in autoimmune and allergic conditions. The objective of this work was to evaluate the effects of TolDC administration in a mouse model of allergic airway inflammation caused by mite extract. Methods: Mouse bone marrow-derived TolDCs were induced by incubation with granulocyte- macrophage colony-stimulating factor (GM-CSF) and dexamethasone, and then characterized by flow cytometry and cytokine production by enzyme- linked immunosorbent assay (ELISA). For the in vivo model of Blomia tropicalis-induced allergy, mice transplanted with antigen-pulsed TolDCs were sensitized intraperitoneally with B. tropicalis mite extract (BtE) adsorbed to aluminium hydroxide. After challenge by nasal administration of BtE, bronchoalveolar lavage fluid (BALF), lungs, spleen and serum were collected for analysis. Results: Induction of TolDCs was efficiently achieved as shown by low expression of major histocompatibility complex (MHC) II, programmed death-ligand (PD-L) 2 and pro-inflammatory cytokine production, and up-regulation of interleukin (IL)-10, upon LPS stimulation in vitro. Transplantation of 1 or 2 doses of BtE-pulsed TolDCs reduced the number of inflammatory cells in BALF and lungs as well as mucus deposition. Moreover, compared to saline-injected controls, TolDC-treated mice showed lower serum levels of anti-BtE immunoglobulin E (IgE) antibodies as well as reduced Gata3 and IL-4 gene expression in the lungs and decreased IFN-γ levels in the supernatant of splenocyte cultures Transplantation of TolDCs increased the percentage of the regulatory T cells in the spleen and the lungs. Conclusions: Preventive treatment with TolDCs protects against dust mite-induced allergy in a mouse model, reinforcing the use of tolerogenic dendritic cells for the management of allergic conditions.engThe Korean Academy of Asthma Allergy and Clinical ImmunologyCélulas dendríticasAsmaAlérgenosÁcaros da poeira domésticaImunoterapiaIndução de tolerânciaDendritic cellsAsthmaAllergensHouse dust mitesImmunotherapyTolerance inductionTolerogenic Dendritic Cells Reduce Airway Inflammation in a Model of Dust Mite Triggered Allergic Inflammationinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/28055/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALAragão- França LS Tolerogenic Dendritic Cells Reduce Airway .....pdfAragão- França 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| dc.title.pt_BR.fl_str_mv |
Tolerogenic Dendritic Cells Reduce Airway Inflammation in a Model of Dust Mite Triggered Allergic Inflammation |
| title |
Tolerogenic Dendritic Cells Reduce Airway Inflammation in a Model of Dust Mite Triggered Allergic Inflammation |
| spellingShingle |
Tolerogenic Dendritic Cells Reduce Airway Inflammation in a Model of Dust Mite Triggered Allergic Inflammation França, Luciana Souza de Aragão Células dendríticas Asma Alérgenos Ácaros da poeira doméstica Imunoterapia Indução de tolerância Dendritic cells Asthma Allergens House dust mites Immunotherapy Tolerance induction |
| title_short |
Tolerogenic Dendritic Cells Reduce Airway Inflammation in a Model of Dust Mite Triggered Allergic Inflammation |
| title_full |
Tolerogenic Dendritic Cells Reduce Airway Inflammation in a Model of Dust Mite Triggered Allergic Inflammation |
| title_fullStr |
Tolerogenic Dendritic Cells Reduce Airway Inflammation in a Model of Dust Mite Triggered Allergic Inflammation |
| title_full_unstemmed |
Tolerogenic Dendritic Cells Reduce Airway Inflammation in a Model of Dust Mite Triggered Allergic Inflammation |
| title_sort |
Tolerogenic Dendritic Cells Reduce Airway Inflammation in a Model of Dust Mite Triggered Allergic Inflammation |
| author |
França, Luciana Souza de Aragão |
| author_facet |
França, Luciana Souza de Aragão Rocha, Viviane Costa Junqueira Andrade, André Cronemberger Costa, F H B Vasconcelos, José Fernandes Athanazio, Daniel Abensur Silva, Daniela Nascimento Santos, Emanuelle de Souza Meira, Cássio Santana Araújo, Cintia Figueiredo de Cerqueira, Jéssica Vieira Cardillo, Fabíola Neves, Neuza Maria Alcântara Soares, Milena Botelho Pereira Pontes-de-Carvalho, Lain Carlos |
| author_role |
author |
| author2 |
Rocha, Viviane Costa Junqueira Andrade, André Cronemberger Costa, F H B Vasconcelos, José Fernandes Athanazio, Daniel Abensur Silva, Daniela Nascimento Santos, Emanuelle de Souza Meira, Cássio Santana Araújo, Cintia Figueiredo de Cerqueira, Jéssica Vieira Cardillo, Fabíola Neves, Neuza Maria Alcântara Soares, Milena Botelho Pereira Pontes-de-Carvalho, Lain Carlos |
| author2_role |
author author author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
França, Luciana Souza de Aragão Rocha, Viviane Costa Junqueira Andrade, André Cronemberger Costa, F H B Vasconcelos, José Fernandes Athanazio, Daniel Abensur Silva, Daniela Nascimento Santos, Emanuelle de Souza Meira, Cássio Santana Araújo, Cintia Figueiredo de Cerqueira, Jéssica Vieira Cardillo, Fabíola Neves, Neuza Maria Alcântara Soares, Milena Botelho Pereira Pontes-de-Carvalho, Lain Carlos |
| dc.subject.other.pt_BR.fl_str_mv |
Células dendríticas Asma Alérgenos Ácaros da poeira doméstica Imunoterapia Indução de tolerância |
| topic |
Células dendríticas Asma Alérgenos Ácaros da poeira doméstica Imunoterapia Indução de tolerância Dendritic cells Asthma Allergens House dust mites Immunotherapy Tolerance induction |
| dc.subject.en.pt_BR.fl_str_mv |
Dendritic cells Asthma Allergens House dust mites Immunotherapy Tolerance induction |
| description |
Programa de Excelência em Pesquisa (PROEP-CNPq). |
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2018 |
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2018-08-10T13:45:55Z |
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2018-08-10T13:45:55Z |
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2018 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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FRANÇA, L. S. A. et al. Tolerogenic Dendritic Cells Reduce Airway Inflammation in a Model of Dust Mite Triggered Allergic Inflammation. Allergy Asthma and Immunology Research, v. 10, n. 4, p. 406-419, 2018. |
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https://www.arca.fiocruz.br/handle/icict/28055 |
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2092-7355 |
| dc.identifier.doi.none.fl_str_mv |
10.4168/aair.2018.10.4.406 |
| identifier_str_mv |
FRANÇA, L. S. A. et al. Tolerogenic Dendritic Cells Reduce Airway Inflammation in a Model of Dust Mite Triggered Allergic Inflammation. Allergy Asthma and Immunology Research, v. 10, n. 4, p. 406-419, 2018. 2092-7355 10.4168/aair.2018.10.4.406 |
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The Korean Academy of Asthma Allergy and Clinical Immunology |
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The Korean Academy of Asthma Allergy and Clinical Immunology |
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