Association of Cardiac Galectin-3 Expression, Myocarditis, and Fibrosis in Chronic Chagas Disease Cardiomyopathy
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2017 |
| Outros Autores: | , , , , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| DOI: | 10.1016/j.ajpath.2017.01.016 |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/25835 |
Resumo: | National Council for Scientific and Technological Development and Bahia Research Foundation. |
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Souza, Bruno Solano de FreitasSilva, Daniela NascimentoCarvalho, Rejane HughesSampaio, Gabriela Louise de AlmeidaParedes, Bruno DiazAragão França, LucianaAzevedo, Carine MachadoVasconcelos, Juliana FragaMeira, Cássio SantanaChenaud Neto, PauloMacambira, Simone GarciaSilva, Kátia Nunes daAllahdadi, Kyan JamesTavora, FabioSouza Neto, João David deSantos, Ricardo Ribeiro dosSoares, Milena Botelho Pereira2018-04-13T14:04:28Z2018-04-13T14:04:28Z2017SOUZA, B. S. F. et al. Association of Cardiac Galectin-3 Expression, Myocarditis, and Fibrosis in Chronic Chagas Disease Cardiomyopathy. American Journal of Pathology, v. 187, n. 5, p. 1134-1146, 2017.0002-9440https://www.arca.fiocruz.br/handle/icict/2583510.1016/j.ajpath.2017.01.016National Council for Scientific and Technological Development and Bahia Research Foundation.Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Hospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFederal University of Bahia. Salvador, BA, Brasil / Messejana Heart and Lung Hospital. Fortaleza, CE, BrazilFederal University of Bahia. Salvador, BA, Brasil / Messejana Heart and Lung Hospital. Fortaleza, CE, BrazilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilChronic Chagas disease cardiomyopathy, caused by Trypanosoma cruzi infection, is a major cause of heart failure in Latin America. Galectin-3 (Gal-3) has been linked to cardiac remodeling and poor prognosis in heart failure of different etiologies. Herein, we investigated the involvement of Gal-3 in the disease pathogenesis and its role as a target for disease intervention. Gal-3 expression in mouse hearts was evaluated during T. cruzi infection by confocal microscopy and flow cytometry analysis, showing a high expression in macrophages, T cells, and fibroblasts. In vitro studies using Gal-3 knockdown in cardiac fibroblasts demonstrated that Gal-3 regulates cell survival, proliferation, and type I collagen synthesis. In vivo blockade of Gal-3 with N-acetyl-d-lactosamine in T. cruzi-infected mice led to a significant reduction of cardiac fibrosis and inflammation in the heart. Moreover, a modulation in the expression of proinflammatory genes in the heart was observed. Finally, histological analysis in human heart samples obtained from subjects with Chagas disease who underwent heart transplantation showed the expression of Gal-3 in areas of inflammation, similar to the mouse model. Our results indicate that Gal-3 plays a role in the pathogenesis of experimental chronic Chagas disease, favoring inflammation and fibrogenesis. Moreover, by demonstrating Gal-3 expression in human hearts, our finding reinforces that this protein could be a novel target for drug development for Chagas cardiomyopathy.engElsevierCardiomiopatia chagásicaGalectina 3Trypanosoma cruziDoença de ChagasInflamaçãoChagasic CardiomyopathyGalectin-3Trypanosoma cruziChagas diseaseInflammationAssociation of Cardiac Galectin-3 Expression, Myocarditis, and Fibrosis in Chronic Chagas Disease Cardiomyopathyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/25835/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALSouza BSLF Association of cardiac....pdfSouza BSLF Association of 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| dc.title.pt_BR.fl_str_mv |
Association of Cardiac Galectin-3 Expression, Myocarditis, and Fibrosis in Chronic Chagas Disease Cardiomyopathy |
| title |
Association of Cardiac Galectin-3 Expression, Myocarditis, and Fibrosis in Chronic Chagas Disease Cardiomyopathy |
| spellingShingle |
Association of Cardiac Galectin-3 Expression, Myocarditis, and Fibrosis in Chronic Chagas Disease Cardiomyopathy Souza, Bruno Solano de Freitas Cardiomiopatia chagásica Galectina 3 Trypanosoma cruzi Doença de Chagas Inflamação Chagasic Cardiomyopathy Galectin-3 Trypanosoma cruzi Chagas disease Inflammation |
| title_short |
Association of Cardiac Galectin-3 Expression, Myocarditis, and Fibrosis in Chronic Chagas Disease Cardiomyopathy |
| title_full |
Association of Cardiac Galectin-3 Expression, Myocarditis, and Fibrosis in Chronic Chagas Disease Cardiomyopathy |
| title_fullStr |
Association of Cardiac Galectin-3 Expression, Myocarditis, and Fibrosis in Chronic Chagas Disease Cardiomyopathy |
| title_full_unstemmed |
Association of Cardiac Galectin-3 Expression, Myocarditis, and Fibrosis in Chronic Chagas Disease Cardiomyopathy |
| title_sort |
Association of Cardiac Galectin-3 Expression, Myocarditis, and Fibrosis in Chronic Chagas Disease Cardiomyopathy |
| author |
Souza, Bruno Solano de Freitas |
| author_facet |
Souza, Bruno Solano de Freitas Silva, Daniela Nascimento Carvalho, Rejane Hughes Sampaio, Gabriela Louise de Almeida Paredes, Bruno Diaz Aragão França, Luciana Azevedo, Carine Machado Vasconcelos, Juliana Fraga Meira, Cássio Santana Chenaud Neto, Paulo Macambira, Simone Garcia Silva, Kátia Nunes da Allahdadi, Kyan James Tavora, Fabio Souza Neto, João David de Santos, Ricardo Ribeiro dos Soares, Milena Botelho Pereira |
| author_role |
author |
| author2 |
Silva, Daniela Nascimento Carvalho, Rejane Hughes Sampaio, Gabriela Louise de Almeida Paredes, Bruno Diaz Aragão França, Luciana Azevedo, Carine Machado Vasconcelos, Juliana Fraga Meira, Cássio Santana Chenaud Neto, Paulo Macambira, Simone Garcia Silva, Kátia Nunes da Allahdadi, Kyan James Tavora, Fabio Souza Neto, João David de Santos, Ricardo Ribeiro dos Soares, Milena Botelho Pereira |
| author2_role |
author author author author author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Souza, Bruno Solano de Freitas Silva, Daniela Nascimento Carvalho, Rejane Hughes Sampaio, Gabriela Louise de Almeida Paredes, Bruno Diaz Aragão França, Luciana Azevedo, Carine Machado Vasconcelos, Juliana Fraga Meira, Cássio Santana Chenaud Neto, Paulo Macambira, Simone Garcia Silva, Kátia Nunes da Allahdadi, Kyan James Tavora, Fabio Souza Neto, João David de Santos, Ricardo Ribeiro dos Soares, Milena Botelho Pereira |
| dc.subject.other.pt_BR.fl_str_mv |
Cardiomiopatia chagásica Galectina 3 Trypanosoma cruzi Doença de Chagas Inflamação |
| topic |
Cardiomiopatia chagásica Galectina 3 Trypanosoma cruzi Doença de Chagas Inflamação Chagasic Cardiomyopathy Galectin-3 Trypanosoma cruzi Chagas disease Inflammation |
| dc.subject.en.pt_BR.fl_str_mv |
Chagasic Cardiomyopathy Galectin-3 Trypanosoma cruzi Chagas disease Inflammation |
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National Council for Scientific and Technological Development and Bahia Research Foundation. |
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2017 |
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2017 |
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2018-04-13T14:04:28Z |
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2018-04-13T14:04:28Z |
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SOUZA, B. S. F. et al. Association of Cardiac Galectin-3 Expression, Myocarditis, and Fibrosis in Chronic Chagas Disease Cardiomyopathy. American Journal of Pathology, v. 187, n. 5, p. 1134-1146, 2017. |
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https://www.arca.fiocruz.br/handle/icict/25835 |
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0002-9440 |
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10.1016/j.ajpath.2017.01.016 |
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SOUZA, B. S. F. et al. Association of Cardiac Galectin-3 Expression, Myocarditis, and Fibrosis in Chronic Chagas Disease Cardiomyopathy. American Journal of Pathology, v. 187, n. 5, p. 1134-1146, 2017. 0002-9440 10.1016/j.ajpath.2017.01.016 |
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