Characterising four Sarconesiopsis magellanica (Diptera: Calliphoridae) larval fat body-derived antimicrobial peptides
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
Texto Completo: | https://www.arca.fiocruz.br/handle/icict/48253 |
Resumo: | Universidad Antonio Nariño, Facultad de Medicina Veterinaria. Bogotá, Colombia. |
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Pérez, CindyDíaz-Roa, AndreaBernal, YuliArenas, Nelson E.Kalume, Dario EluanCôrtes, Luzia Monteiro de CastroSilva Junior, Pedro I. daVarela, YahsonPatarroyo, Manuel A.Torres, OrlandoBello, Felio J.2021-07-16T12:43:21Z2021-07-16T12:43:21Z2021PÉREZ, Cindy et al. Characterising four Sarconesiopsis magellanica (Diptera: Calliphoridae) larval fat body-derived antimicrobial peptides. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 116, e200587, 11 p, 2021.0074-0276https://www.arca.fiocruz.br/handle/icict/4825310.1590/0074-02760200587engFundação Oswaldo Cruz. Instituto Oswaldo Cruz.Sarconesiopsis magellanicaPeptídeos antimicrobianosCorpo de gordura larvalCaracterização físico-químicaAvaliação antibacterianaSarconesiopsis magellanicaAntimicrobial peptidesLarval fat bodyPhysicochemical characterisationAntibacterial evaluationCharacterising four Sarconesiopsis magellanica (Diptera: Calliphoridae) larval fat body-derived antimicrobial peptidesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidad Antonio Nariño, Facultad de Medicina Veterinaria. Bogotá, Colombia.Instituto Butantan, Laboratório de Toxinología Aplicada. São Paulo, SP, Brasil / Universidad Nacional Abierta y a Distancia, Escuela de Ciencias Agrícolas, Pecuarias y de Medio Ambiente. Bogotá, Colombia.Universidad Antonio Nariño, Facultad de Ciencias. Bogotá, Colombia.Universidad Antonio Nariño, Facultad de Ciencias. Bogotá, Colombia.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil.Instituto Butantan, Laboratório de Toxinología Aplicada. São Paulo, SP, Brasil.Fundación Instituto de Inmunología de Colombia, Molecular Biology and Immunology Department. Bogotá, Colombia.Fundación Instituto de Inmunología de Colombia, Molecular Biology and Immunology Department. Bogotá, Colombia / Universidad Nacional de Colombia, Faculty of Medicine, Microbiology Department, Bogotá, Colombia / Universidad Santo Tomás, Health Sciences Division. Bogotá, Colombia.Universidad Antonio Nariño, Facultad de Medicina Veterinaria. Bogotá, Colombia.Universidad de La Salle, Facultad de Ciencias Agropecuarias, Programa de Medicina Veterinaria. Bogotá, Colombia.BACKGROUND The inappropriate use of antibiotics has led to the accelerated growth of resistance to antibiotics. The search for new therapeutic strategies (i.e., antimicrobial peptides-AMPs) has thus become a pressing need. OBJECTIVE Characterising and evaluating Sarconesiopsis magellanica larval fat body-derived AMPs. METHODS Fat body extracts were analysed by reversed-phase high-performance liquid chromatography (RP-HPLC); mass spectrometry was used for characterising the primary structure of the AMPs so found. ProtParam (Expasy) was used for analysing the AMPs’ physico-chemical properties. Synthetic AMPs’ antibacterial activity was evaluated. FINDINGS Four new AMPs were obtained and called sarconesin III, IV, V and VI. Sarconesin III had an α-helix structure and sarconesins IV, V and VI had linear formations. Oligomer prediction highlighted peptide-peptide interactions, suggesting that sarconesins III, V and VI could form self-aggregations when in contact with the microbial membrane. AMPs synthesised from their native molecules’ sequences had potent activity against Gram-positive bacteria and, to a lesser extent, against Gram-negative and drug-resistant bacteria. Sarconesin VI was the most efficient AMP. None of the four synthetic AMPs had a cytotoxic effect. MAIN CONCLUSIONS S. magellanica larval fat body-derived antimicrobial peptides are an important source of AMPs and could be used in different antimicrobial therapies and overcoming bacterial resistance,info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/48253/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALDarioKalume_LuisaCortes_etal_IOC_1921.pdfDarioKalume_LuisaCortes_etal_IOC_1921.pdfapplication/pdf2001336https://www.arca.fiocruz.br/bitstream/icict/48253/2/DarioKalume_LuisaCortes_etal_IOC_1921.pdfc19ad1b1cdb5730344789bf84ab842d2MD52TEXTDarioKalume_LuisaCortes_etal_IOC_1921.pdf.txtDarioKalume_LuisaCortes_etal_IOC_1921.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv |
Characterising four Sarconesiopsis magellanica (Diptera: Calliphoridae) larval fat body-derived antimicrobial peptides |
title |
Characterising four Sarconesiopsis magellanica (Diptera: Calliphoridae) larval fat body-derived antimicrobial peptides |
spellingShingle |
Characterising four Sarconesiopsis magellanica (Diptera: Calliphoridae) larval fat body-derived antimicrobial peptides Pérez, Cindy Sarconesiopsis magellanica Peptídeos antimicrobianos Corpo de gordura larval Caracterização físico-química Avaliação antibacteriana Sarconesiopsis magellanica Antimicrobial peptides Larval fat body Physicochemical characterisation Antibacterial evaluation |
title_short |
Characterising four Sarconesiopsis magellanica (Diptera: Calliphoridae) larval fat body-derived antimicrobial peptides |
title_full |
Characterising four Sarconesiopsis magellanica (Diptera: Calliphoridae) larval fat body-derived antimicrobial peptides |
title_fullStr |
Characterising four Sarconesiopsis magellanica (Diptera: Calliphoridae) larval fat body-derived antimicrobial peptides |
title_full_unstemmed |
Characterising four Sarconesiopsis magellanica (Diptera: Calliphoridae) larval fat body-derived antimicrobial peptides |
title_sort |
Characterising four Sarconesiopsis magellanica (Diptera: Calliphoridae) larval fat body-derived antimicrobial peptides |
author |
Pérez, Cindy |
author_facet |
Pérez, Cindy Díaz-Roa, Andrea Bernal, Yuli Arenas, Nelson E. Kalume, Dario Eluan Côrtes, Luzia Monteiro de Castro Silva Junior, Pedro I. da Varela, Yahson Patarroyo, Manuel A. Torres, Orlando Bello, Felio J. |
author_role |
author |
author2 |
Díaz-Roa, Andrea Bernal, Yuli Arenas, Nelson E. Kalume, Dario Eluan Côrtes, Luzia Monteiro de Castro Silva Junior, Pedro I. da Varela, Yahson Patarroyo, Manuel A. Torres, Orlando Bello, Felio J. |
author2_role |
author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Pérez, Cindy Díaz-Roa, Andrea Bernal, Yuli Arenas, Nelson E. Kalume, Dario Eluan Côrtes, Luzia Monteiro de Castro Silva Junior, Pedro I. da Varela, Yahson Patarroyo, Manuel A. Torres, Orlando Bello, Felio J. |
dc.subject.other.pt_BR.fl_str_mv |
Sarconesiopsis magellanica Peptídeos antimicrobianos Corpo de gordura larval Caracterização físico-química Avaliação antibacteriana |
topic |
Sarconesiopsis magellanica Peptídeos antimicrobianos Corpo de gordura larval Caracterização físico-química Avaliação antibacteriana Sarconesiopsis magellanica Antimicrobial peptides Larval fat body Physicochemical characterisation Antibacterial evaluation |
dc.subject.en.pt_BR.fl_str_mv |
Sarconesiopsis magellanica Antimicrobial peptides Larval fat body Physicochemical characterisation Antibacterial evaluation |
description |
Universidad Antonio Nariño, Facultad de Medicina Veterinaria. Bogotá, Colombia. |
publishDate |
2021 |
dc.date.accessioned.fl_str_mv |
2021-07-16T12:43:21Z |
dc.date.available.fl_str_mv |
2021-07-16T12:43:21Z |
dc.date.issued.fl_str_mv |
2021 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
PÉREZ, Cindy et al. Characterising four Sarconesiopsis magellanica (Diptera: Calliphoridae) larval fat body-derived antimicrobial peptides. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 116, e200587, 11 p, 2021. |
dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/48253 |
dc.identifier.issn.pt_BR.fl_str_mv |
0074-0276 |
dc.identifier.doi.none.fl_str_mv |
10.1590/0074-02760200587 |
identifier_str_mv |
PÉREZ, Cindy et al. Characterising four Sarconesiopsis magellanica (Diptera: Calliphoridae) larval fat body-derived antimicrobial peptides. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 116, e200587, 11 p, 2021. 0074-0276 10.1590/0074-02760200587 |
url |
https://www.arca.fiocruz.br/handle/icict/48253 |
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eng |
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eng |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. |
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Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. |
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