A purification methodology for viable and infective Plasmodium vivax gametocytes compatible with transmission blocking assays

Detalhes bibliográficos
Autor(a) principal: Vera, Omaira
Data de Publicação: 2015
Outros Autores: Brito, Paula Brelas de, Albrecht, Letusa, Campos, Keillen Monick Martins, Pimenta, Paulo Filemon Paolucci, Monteiro, Wuelton Marcelo, Lacerda, Marcus Vinícius Guimarães de, Lopes, Stefanie Costa Pinto, Costa, Fabio Trindade Maranhão
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/13766
Resumo: Universidade Estadual de Campinas. Departamento de Genética, Evolução e Bioagentes. Campinas, SP, Brasil / Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Gerência de Malária. Manaus, AM, Brasil.
id CRUZ_a3c66bcd6ec6ae0e7c501ba3d07d4b31
oai_identifier_str oai:www.arca.fiocruz.br:icict/13766
network_acronym_str CRUZ
network_name_str Repositório Institucional da FIOCRUZ (ARCA)
repository_id_str 2135
spelling Vera, OmairaBrito, Paula Brelas deAlbrecht, LetusaCampos, Keillen Monick MartinsPimenta, Paulo Filemon PaolucciMonteiro, Wuelton MarceloLacerda, Marcus Vinícius Guimarães deLopes, Stefanie Costa PintoCosta, Fabio Trindade Maranhão2016-04-14T14:16:35Z2016-04-14T14:16:35Z2015VERA, Omaira et al. A purification methodology for viable and infective Plasmodium vivax gametocytes compatible with transmission blocking assays. Antimicrobial Agents and Chemotherapy, v. 59, n. 10, p. 6638-6641, 2015.0066-4804https://www.arca.fiocruz.br/handle/icict/1376610.1128/AAC.01136-15engAmerican Society for MicrobiologyA purification methodology for viable and infective Plasmodium vivax gametocytes compatible with transmission blocking assaysinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Estadual de Campinas. Departamento de Genética, Evolução e Bioagentes. Campinas, SP, Brasil / Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Gerência de Malária. Manaus, AM, Brasil.Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Gerência de Malária. Manaus, AM, Brasil.Universidade Estadual de Campinas. Departamento de Genética, Evolução e Bioagentes. Campinas, SP, Brasil / Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Gerência de Malária. Manaus, AM, Brasil / Universidade do Estado do Amazonas. Escola Superior de Ciências da Saúde. Manaus, AM, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil.Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Gerência de Malária. Manaus, AM, Brasil / Universidade do Estado do Amazonas. Escola Superior de Ciências da Saúde. Manaus, AM, Brasil.Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Gerência de Malária. Manaus, AM, Brasil / Universidade do Estado do Amazonas. Escola Superior de Ciências da Saúde. Manaus, AM, Brasil.Universidade Estadual de Campinas. Departamento de Genética, Evolução e Bioagentes. Campinas, SP, Brasil /Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Gerência de Malária. Manaus, AM, Brasil / Fundação Oswaldo Cruz. Instituto Leônidas e Maria Deane Manaus, AM, Brasil.Universidade Estadual de Campinas. Departamento de Genética, Evolução e Bioagentes. Campinas, SP, Brasil.Significant progress toward the control of malaria has been achieved, especially regarding Plasmodium falciparum infections. However, the unique biology of Plasmodium vivax hampers current control strategies. The early appearance of P. vivax gametocytes in the peripheral blood and the impossibility of culturing this parasite are major drawbacks. Using blood samples from 40 P. vivax-infected patients, we describe here a methodology to purify viable gametocytes and further infect anophelines. This method opens new avenues to validate transmission-blocking strategies.MalariaPlasmodium falciparuminfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/13766/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALPurification Methodology for Viable and Infective Plasmodium vivax Gametocytes That Is Compatible with Transmission-Blocking Assays.pdfPurification Methodology for Viable and Infective Plasmodium vivax Gametocytes That Is Compatible with Transmission-Blocking Assays.pdfapplication/pdf2142369https://www.arca.fiocruz.br/bitstream/icict/13766/2/Purification%20Methodology%20for%20Viable%20and%20Infective%20Plasmodium%20vivax%20Gametocytes%20That%20Is%20Compatible%20with%20Transmission-Blocking%20Assays.pdf94b589033cc0703ac1de355e61f77153MD52TEXTPurification Methodology for Viable and Infective Plasmodium vivax Gametocytes That Is Compatible with Transmission-Blocking Assays.pdf.txtPurification Methodology for Viable and Infective Plasmodium vivax Gametocytes That Is Compatible with Transmission-Blocking Assays.pdf.txtExtracted texttext/plain4https://www.arca.fiocruz.br/bitstream/icict/13766/3/Purification%20Methodology%20for%20Viable%20and%20Infective%20Plasmodium%20vivax%20Gametocytes%20That%20Is%20Compatible%20with%20Transmission-Blocking%20Assays.pdf.txtff4c8ff01d544500ea4bfea43e6108c1MD53icict/137662019-12-15 19:36:19.801oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352019-12-15T22:36:19Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv A purification methodology for viable and infective Plasmodium vivax gametocytes compatible with transmission blocking assays
title A purification methodology for viable and infective Plasmodium vivax gametocytes compatible with transmission blocking assays
spellingShingle A purification methodology for viable and infective Plasmodium vivax gametocytes compatible with transmission blocking assays
Vera, Omaira
Malaria
Plasmodium falciparum
title_short A purification methodology for viable and infective Plasmodium vivax gametocytes compatible with transmission blocking assays
title_full A purification methodology for viable and infective Plasmodium vivax gametocytes compatible with transmission blocking assays
title_fullStr A purification methodology for viable and infective Plasmodium vivax gametocytes compatible with transmission blocking assays
title_full_unstemmed A purification methodology for viable and infective Plasmodium vivax gametocytes compatible with transmission blocking assays
title_sort A purification methodology for viable and infective Plasmodium vivax gametocytes compatible with transmission blocking assays
author Vera, Omaira
author_facet Vera, Omaira
Brito, Paula Brelas de
Albrecht, Letusa
Campos, Keillen Monick Martins
Pimenta, Paulo Filemon Paolucci
Monteiro, Wuelton Marcelo
Lacerda, Marcus Vinícius Guimarães de
Lopes, Stefanie Costa Pinto
Costa, Fabio Trindade Maranhão
author_role author
author2 Brito, Paula Brelas de
Albrecht, Letusa
Campos, Keillen Monick Martins
Pimenta, Paulo Filemon Paolucci
Monteiro, Wuelton Marcelo
Lacerda, Marcus Vinícius Guimarães de
Lopes, Stefanie Costa Pinto
Costa, Fabio Trindade Maranhão
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Vera, Omaira
Brito, Paula Brelas de
Albrecht, Letusa
Campos, Keillen Monick Martins
Pimenta, Paulo Filemon Paolucci
Monteiro, Wuelton Marcelo
Lacerda, Marcus Vinícius Guimarães de
Lopes, Stefanie Costa Pinto
Costa, Fabio Trindade Maranhão
dc.subject.en.pt_BR.fl_str_mv Malaria
Plasmodium falciparum
topic Malaria
Plasmodium falciparum
description Universidade Estadual de Campinas. Departamento de Genética, Evolução e Bioagentes. Campinas, SP, Brasil / Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Gerência de Malária. Manaus, AM, Brasil.
publishDate 2015
dc.date.issued.fl_str_mv 2015
dc.date.accessioned.fl_str_mv 2016-04-14T14:16:35Z
dc.date.available.fl_str_mv 2016-04-14T14:16:35Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv VERA, Omaira et al. A purification methodology for viable and infective Plasmodium vivax gametocytes compatible with transmission blocking assays. Antimicrobial Agents and Chemotherapy, v. 59, n. 10, p. 6638-6641, 2015.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/13766
dc.identifier.issn.none.fl_str_mv 0066-4804
dc.identifier.doi.none.fl_str_mv 10.1128/AAC.01136-15
identifier_str_mv VERA, Omaira et al. A purification methodology for viable and infective Plasmodium vivax gametocytes compatible with transmission blocking assays. Antimicrobial Agents and Chemotherapy, v. 59, n. 10, p. 6638-6641, 2015.
0066-4804
10.1128/AAC.01136-15
url https://www.arca.fiocruz.br/handle/icict/13766
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/13766/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/13766/2/Purification%20Methodology%20for%20Viable%20and%20Infective%20Plasmodium%20vivax%20Gametocytes%20That%20Is%20Compatible%20with%20Transmission-Blocking%20Assays.pdf
https://www.arca.fiocruz.br/bitstream/icict/13766/3/Purification%20Methodology%20for%20Viable%20and%20Infective%20Plasmodium%20vivax%20Gametocytes%20That%20Is%20Compatible%20with%20Transmission-Blocking%20Assays.pdf.txt
bitstream.checksum.fl_str_mv 5a560609d32a3863062d77ff32785d58
94b589033cc0703ac1de355e61f77153
ff4c8ff01d544500ea4bfea43e6108c1
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
_version_ 1813009080831180800

Registros relacionados