Comparative analysis of systemic and tumor microenvironment proteomes from children with B-cell acute lymphocytic leukemia at diagnosis and after induction treatment

Detalhes bibliográficos
Autor(a) principal: Broto, Geise Ellen
Data de Publicação: 2020
Outros Autores: Corrêa, Stephany, Trigo, Fausto Celso, Santos, Everton Cruz dos, Tomiotto-Pelissier, Fernanda, Pavanelli, Wander Rogério, Silveira, Guilherme Ferreira, Abdelhay, Eliana Saul Furquim Werneck, Panis, Carolina
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/45768
Resumo: Universidade Estadual de Londrina. Programa de Pós-graduação em Patologia Clínica e Laboratorial. Londrina, PR, Brasil. / Universidade Estadual do Oeste do Paraná. Laboratório de Biologia de Tumores. Francisco Beltrão, PR, Brasil.
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spelling Broto, Geise EllenCorrêa, StephanyTrigo, Fausto CelsoSantos, Everton Cruz dosTomiotto-Pelissier, FernandaPavanelli, Wander RogérioSilveira, Guilherme FerreiraAbdelhay, Eliana Saul Furquim WerneckPanis, Carolina2021-01-21T17:05:55Z2021-01-21T17:05:55Z2020BROTO, Geise Ellen et al. Comparative analysis of systemic and tumor microenvironment proteomes from children with B-cell acute lymphocytic leukemia at diagnosis and after induction treatment Frontiers in Oncology, v.10, n. 550213, p. 1–13, 2020.2234-943Xhttps://www.arca.fiocruz.br/handle/icict/4576810.3389/fonc.2020.550213engFrontiers MediaComparative analysis of systemic and tumor microenvironment proteomes from children with B-cell acute lymphocytic leukemia at diagnosis and after induction treatmentinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Estadual de Londrina. Programa de Pós-graduação em Patologia Clínica e Laboratorial. Londrina, PR, Brasil. / Universidade Estadual do Oeste do Paraná. Laboratório de Biologia de Tumores. Francisco Beltrão, PR, Brasil.Instituto Nacional de Câncer. Centro de Transplante de Medula Óssea. Laboratório de Células-Tronco. Rio de Janeiro, RJ, Brasil.Hospital do Câncer de Londrina. Departamento de Pediatria. Londrina, PR, Brasil.Instituto Nacional de Câncer. Centro de Transplante de Medula Óssea. Laboratório de Células-Tronco. Rio de Janeiro, RJ, Brasil.Universidade Estadual de Londrina. Programa de Pós-graduação em Patologia Experimental. Londrina, PR, Brasil.Universidade Estadual de Londrina. Programa de Pós-graduação em Patologia Experimental. Londrina, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Instituto Nacional de Câncer. Centro de Transplante de Medula Óssea. Laboratório de Células-Tronco. Rio de Janeiro, RJ, Brasil.Universidade Estadual de Londrina. Programa de Pós-graduação em Patologia Clínica e Laboratorial. Londrina, PR, Brasil. / Universidade Estadual do Oeste do Paraná. Laboratório de Biologia de Tumores. Francisco Beltrão, PR, Brasil. / Universidade Estadual de Londrina. Programa de Pós-graduação em Patologia Experimental. Londrina, PR, Brasil. / Universidade Estadual do Oeste do Paraná. Programa de Pós-Graduação em Ciências Aplicadas à Saúde. Francisco Beltrão, PR, Brasil.Among the childhood diseases, B-cell acute lymphocytic leukemia (B-ALL) is the most frequent type of cancer. Despite recent advances concerning disease treatment, cytotoxic chemotherapy remains the first line of treatment in several countries, and the modifications induced by such drugs in the organism are still poorly understood. In this context, the present study provided a comparative high-throughput proteomic analysis of the cumulative changes induced by chemotherapeutic drugs used in the induction phase of B-ALL treatment in both peripheral blood (PB) and bone marrow compartment (BM) samples. To reach this goal, PB and BM plasma samples were comparatively analyzed by using label-free proteomics at two endpoints: at diagnosis (D0) and the end of the cumulative induction phase treatment (D28). Proteomic data was available via ProteomeXchange with identifier PXD021584. The resulting differentially expressed proteins were explored by bioinformatics approaches aiming to identify the main gene ontology processes, pathways, and transcription factors altered by chemotherapy, as well as to understand B-ALL biology in each compartment at D0. At D0, PB was characterized as a pro-inflammatory environment, with the involvement of several downregulated coagulation proteins as KNG, plasmin, and plasminogen. D28 was characterized predominantly by immune response-related processes and the super expression of the transcription factor IRF3 and transthyretin. RUNX1 was pointed out as a common transcription factor found in both D0 and D28. We chose to validate the proteins transthyretin and interferon-gamma (IFN-g) by commercial kits and expressed the results as PB/BM ratios. Transthyretin ratio was augmented after induction chemotherapy, while IFN-g was reduced at the end of the treatment. Considering that most of these proteins were not yet described in B-ALL literature, these findings added to understanding disease biology at diagnosis and highlighted a possible role for transthyretin and IFN-g as mechanisms related to disease resolution.Interferon gamaInterferon Regulatory Factor-3transthyretinLeukemia, Biphenotypic, AcuteProteomicsInterferón gammaFactor 3 Regulador del InterferónPrealbúminaLeucemia Bifenotípica AgudaProteómicaInterféron gammaFacteur-3 de régulation d'interféronPréalbumineLeucémie aigüe biphénotypiqueProtéomiqueInterferon gamaFator Regulador 3 de InterferonPré-AlbuminaLeucemia Aguda BifenotípicaProteômicainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; 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dc.title.pt_BR.fl_str_mv Comparative analysis of systemic and tumor microenvironment proteomes from children with B-cell acute lymphocytic leukemia at diagnosis and after induction treatment
title Comparative analysis of systemic and tumor microenvironment proteomes from children with B-cell acute lymphocytic leukemia at diagnosis and after induction treatment
spellingShingle Comparative analysis of systemic and tumor microenvironment proteomes from children with B-cell acute lymphocytic leukemia at diagnosis and after induction treatment
Broto, Geise Ellen
Interferon gama
Interferon Regulatory Factor-3
transthyretin
Leukemia, Biphenotypic, Acute
Proteomics
Interferón gamma
Factor 3 Regulador del Interferón
Prealbúmina
Leucemia Bifenotípica Aguda
Proteómica
Interféron gamma
Facteur-3 de régulation d'interféron
Préalbumine
Leucémie aigüe biphénotypique
Protéomique
Interferon gama
Fator Regulador 3 de Interferon
Pré-Albumina
Leucemia Aguda Bifenotípica
Proteômica
title_short Comparative analysis of systemic and tumor microenvironment proteomes from children with B-cell acute lymphocytic leukemia at diagnosis and after induction treatment
title_full Comparative analysis of systemic and tumor microenvironment proteomes from children with B-cell acute lymphocytic leukemia at diagnosis and after induction treatment
title_fullStr Comparative analysis of systemic and tumor microenvironment proteomes from children with B-cell acute lymphocytic leukemia at diagnosis and after induction treatment
title_full_unstemmed Comparative analysis of systemic and tumor microenvironment proteomes from children with B-cell acute lymphocytic leukemia at diagnosis and after induction treatment
title_sort Comparative analysis of systemic and tumor microenvironment proteomes from children with B-cell acute lymphocytic leukemia at diagnosis and after induction treatment
author Broto, Geise Ellen
author_facet Broto, Geise Ellen
Corrêa, Stephany
Trigo, Fausto Celso
Santos, Everton Cruz dos
Tomiotto-Pelissier, Fernanda
Pavanelli, Wander Rogério
Silveira, Guilherme Ferreira
Abdelhay, Eliana Saul Furquim Werneck
Panis, Carolina
author_role author
author2 Corrêa, Stephany
Trigo, Fausto Celso
Santos, Everton Cruz dos
Tomiotto-Pelissier, Fernanda
Pavanelli, Wander Rogério
Silveira, Guilherme Ferreira
Abdelhay, Eliana Saul Furquim Werneck
Panis, Carolina
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Broto, Geise Ellen
Corrêa, Stephany
Trigo, Fausto Celso
Santos, Everton Cruz dos
Tomiotto-Pelissier, Fernanda
Pavanelli, Wander Rogério
Silveira, Guilherme Ferreira
Abdelhay, Eliana Saul Furquim Werneck
Panis, Carolina
dc.subject.en.pt_BR.fl_str_mv Interferon gama
Interferon Regulatory Factor-3
transthyretin
Leukemia, Biphenotypic, Acute
Proteomics
topic Interferon gama
Interferon Regulatory Factor-3
transthyretin
Leukemia, Biphenotypic, Acute
Proteomics
Interferón gamma
Factor 3 Regulador del Interferón
Prealbúmina
Leucemia Bifenotípica Aguda
Proteómica
Interféron gamma
Facteur-3 de régulation d'interféron
Préalbumine
Leucémie aigüe biphénotypique
Protéomique
Interferon gama
Fator Regulador 3 de Interferon
Pré-Albumina
Leucemia Aguda Bifenotípica
Proteômica
dc.subject.es.pt_BR.fl_str_mv Interferón gamma
Factor 3 Regulador del Interferón
Prealbúmina
Leucemia Bifenotípica Aguda
Proteómica
dc.subject.fr.pt_BR.fl_str_mv Interféron gamma
Facteur-3 de régulation d'interféron
Préalbumine
Leucémie aigüe biphénotypique
Protéomique
dc.subject.decs.pt_BR.fl_str_mv Interferon gama
Fator Regulador 3 de Interferon
Pré-Albumina
Leucemia Aguda Bifenotípica
Proteômica
description Universidade Estadual de Londrina. Programa de Pós-graduação em Patologia Clínica e Laboratorial. Londrina, PR, Brasil. / Universidade Estadual do Oeste do Paraná. Laboratório de Biologia de Tumores. Francisco Beltrão, PR, Brasil.
publishDate 2020
dc.date.issued.fl_str_mv 2020
dc.date.accessioned.fl_str_mv 2021-01-21T17:05:55Z
dc.date.available.fl_str_mv 2021-01-21T17:05:55Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv BROTO, Geise Ellen et al. Comparative analysis of systemic and tumor microenvironment proteomes from children with B-cell acute lymphocytic leukemia at diagnosis and after induction treatment Frontiers in Oncology, v.10, n. 550213, p. 1–13, 2020.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/45768
dc.identifier.issn.pt_BR.fl_str_mv 2234-943X
dc.identifier.doi.none.fl_str_mv 10.3389/fonc.2020.550213
identifier_str_mv BROTO, Geise Ellen et al. Comparative analysis of systemic and tumor microenvironment proteomes from children with B-cell acute lymphocytic leukemia at diagnosis and after induction treatment Frontiers in Oncology, v.10, n. 550213, p. 1–13, 2020.
2234-943X
10.3389/fonc.2020.550213
url https://www.arca.fiocruz.br/handle/icict/45768
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/45768/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/45768/2/fonc-10-550213Oi.pdf
https://www.arca.fiocruz.br/bitstream/icict/45768/3/fonc-10-550213Oi.pdf.txt
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
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