Comparative study of RFLP-IS6110 and MIRU-VNTR from Mycobacterium tuberculosis isolated in the state of Minas Gerais, Brazil

Detalhes bibliográficos
Autor(a) principal: Augusto, Cláudio José
Data de Publicação: 2018
Outros Autores: Carvalho, Wânia da Silva, Almeida, Isabela Neves de, Figueiredo, Lida Jouca de Assis, Dantas, Nayanne Gama Teixeira, Suffys, Philip Noel, Miranda, Silvana Spíndola de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/36951
Resumo: Fundação Ezequiel Dias. Belo Horizonte, MG, Brasil / Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil.
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spelling Augusto, Cláudio JoséCarvalho, Wânia da SilvaAlmeida, Isabela Neves deFigueiredo, Lida Jouca de AssisDantas, Nayanne Gama TeixeiraSuffys, Philip NoelMiranda, Silvana Spíndola de2019-11-07T17:44:00Z2019-11-07T17:44:00Z2018AUGUSTO, Cláudio José et al. Comparative study of RFLP-IS6110 and MIRU-VNTR from Mycobacterium tuberculosis isolated in the state of Minas Gerais, Brazil. Brazilian Journal of Microbiology, v. 49, p. 641-646, 2018.1517-8382https://www.arca.fiocruz.br/handle/icict/3695110.1016/j.bjm.2017.04.0151678-4405engSociedade Brasileira de MicrobiologiaMycobacterium tuberculosisMinas GeraisMycobacterium tuberculosisRFLPVNTR lociBrazilComparative study of RFLP-IS6110 and MIRU-VNTR from Mycobacterium tuberculosis isolated in the state of Minas Gerais, Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Ezequiel Dias. Belo Horizonte, MG, Brasil / Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular Aplicada a Micobactérias. Rio de Janeiro, RJ, Brasil.Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil.tDNA genotyping of Mycobacterium tuberculosis has been widely applied in the understandingof disease transmission in many countries. The purpose of this study was to genotype thestrains of M. tuberculosis isolated in patients with new tuberculosis (TB) cases in Minas Gerais,as well as to compare the similarity, discriminatory power, and agreement of the clustersbetween the IS6110 Restriction Fragment Length Polymorfism (RFLP) and 12 loci VariableNumber Tandem Repeat – Mycobacterial Interspersed Repetitive Units (MIRU-VNTR) tech-niques. It was observed that 32% (66/204) of the isolated strains in the RFLP-IS6110 and50.9% (104/204) of the isolated strains in the MIRU-VNTR presented a similarity of equal toor above 85%. The RFLP-IS6110 and MIRU-VNTR proved to contain a high discriminatorypower. The similarity index resulting from the RFLP showed no recent transmission. Goodagreement was observed between the techniques when clusters were detected; however,the best epidemiological relationship was found when using the RFLP-IS6110.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/36951/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALPhilipNSuffys_etal_IOC_2018.pdfPhilipNSuffys_etal_IOC_2018.pdfapplication/pdf1248759https://www.arca.fiocruz.br/bitstream/icict/36951/2/PhilipNSuffys_etal_IOC_2018.pdf4434625f721630715ccb3e005e095490MD52TEXTPhilipNSuffys_etal_IOC_2018.pdf.txtPhilipNSuffys_etal_IOC_2018.pdf.txtExtracted texttext/plain28158https://www.arca.fiocruz.br/bitstream/icict/36951/3/PhilipNSuffys_etal_IOC_2018.pdf.txta6d8d6c9af6ab13cc5f0d67bb795efa1MD53icict/369512019-12-17 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dc.title.pt_BR.fl_str_mv Comparative study of RFLP-IS6110 and MIRU-VNTR from Mycobacterium tuberculosis isolated in the state of Minas Gerais, Brazil
title Comparative study of RFLP-IS6110 and MIRU-VNTR from Mycobacterium tuberculosis isolated in the state of Minas Gerais, Brazil
spellingShingle Comparative study of RFLP-IS6110 and MIRU-VNTR from Mycobacterium tuberculosis isolated in the state of Minas Gerais, Brazil
Augusto, Cláudio José
Mycobacterium tuberculosis
Minas Gerais
Mycobacterium tuberculosis
RFLP
VNTR loci
Brazil
title_short Comparative study of RFLP-IS6110 and MIRU-VNTR from Mycobacterium tuberculosis isolated in the state of Minas Gerais, Brazil
title_full Comparative study of RFLP-IS6110 and MIRU-VNTR from Mycobacterium tuberculosis isolated in the state of Minas Gerais, Brazil
title_fullStr Comparative study of RFLP-IS6110 and MIRU-VNTR from Mycobacterium tuberculosis isolated in the state of Minas Gerais, Brazil
title_full_unstemmed Comparative study of RFLP-IS6110 and MIRU-VNTR from Mycobacterium tuberculosis isolated in the state of Minas Gerais, Brazil
title_sort Comparative study of RFLP-IS6110 and MIRU-VNTR from Mycobacterium tuberculosis isolated in the state of Minas Gerais, Brazil
author Augusto, Cláudio José
author_facet Augusto, Cláudio José
Carvalho, Wânia da Silva
Almeida, Isabela Neves de
Figueiredo, Lida Jouca de Assis
Dantas, Nayanne Gama Teixeira
Suffys, Philip Noel
Miranda, Silvana Spíndola de
author_role author
author2 Carvalho, Wânia da Silva
Almeida, Isabela Neves de
Figueiredo, Lida Jouca de Assis
Dantas, Nayanne Gama Teixeira
Suffys, Philip Noel
Miranda, Silvana Spíndola de
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Augusto, Cláudio José
Carvalho, Wânia da Silva
Almeida, Isabela Neves de
Figueiredo, Lida Jouca de Assis
Dantas, Nayanne Gama Teixeira
Suffys, Philip Noel
Miranda, Silvana Spíndola de
dc.subject.other.pt_BR.fl_str_mv Mycobacterium tuberculosis
Minas Gerais
topic Mycobacterium tuberculosis
Minas Gerais
Mycobacterium tuberculosis
RFLP
VNTR loci
Brazil
dc.subject.en.pt_BR.fl_str_mv Mycobacterium tuberculosis
RFLP
VNTR loci
Brazil
description Fundação Ezequiel Dias. Belo Horizonte, MG, Brasil / Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil.
publishDate 2018
dc.date.issued.fl_str_mv 2018
dc.date.accessioned.fl_str_mv 2019-11-07T17:44:00Z
dc.date.available.fl_str_mv 2019-11-07T17:44:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv AUGUSTO, Cláudio José et al. Comparative study of RFLP-IS6110 and MIRU-VNTR from Mycobacterium tuberculosis isolated in the state of Minas Gerais, Brazil. Brazilian Journal of Microbiology, v. 49, p. 641-646, 2018.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/36951
dc.identifier.issn.pt_BR.fl_str_mv 1517-8382
dc.identifier.doi.none.fl_str_mv 10.1016/j.bjm.2017.04.015
dc.identifier.eissn.none.fl_str_mv 1678-4405
identifier_str_mv AUGUSTO, Cláudio José et al. Comparative study of RFLP-IS6110 and MIRU-VNTR from Mycobacterium tuberculosis isolated in the state of Minas Gerais, Brazil. Brazilian Journal of Microbiology, v. 49, p. 641-646, 2018.
1517-8382
10.1016/j.bjm.2017.04.015
1678-4405
url https://www.arca.fiocruz.br/handle/icict/36951
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
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instname_str Fundação Oswaldo Cruz (FIOCRUZ)
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institution FIOCRUZ
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