Metacyclogenesis defects and gene expression hallmarks of histone deacetylase 4‑deficient Trypanosoma cruzi cells

Detalhes bibliográficos
Autor(a) principal: Picchi‑Constante, Gisele Fernanda Assine
Data de Publicação: 2021
Outros Autores: Guerra‑Slompo, Eloise Pavão, Tahira, Ana Carolina, Alcantara, Monica Visnieski, Amaral, Murilo Sena, Ferreira, Arthur Schveitzer, Batista, Michel, Batista, Cassiano Martin, Goldenberg, Samuel, Verjovski‑Almeida, Sergio, Zanchin, Nilson Ivo Tonin
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/50054
Resumo: Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.
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spelling Picchi‑Constante, Gisele Fernanda AssineGuerra‑Slompo, Eloise PavãoTahira, Ana CarolinaAlcantara, Monica VisnieskiAmaral, Murilo SenaFerreira, Arthur SchveitzerBatista, MichelBatista, Cassiano MartinGoldenberg, SamuelVerjovski‑Almeida, SergioZanchin, Nilson Ivo Tonin2021-11-24T19:02:33Z2021-11-24T19:02:33Z2021PICCHI-CONSTANTE, Gisele Fernanda Assine et al. Metacyclogenesis defects and gene expression hallmarks of histone deacetylase 4‑deficient Trypanosoma cruzi cells. Scientific Reports, v.11, n. 21671, p. 1–18, 2021.2045-2322https://www.arca.fiocruz.br/handle/icict/5005410.1038/s41598-021-01080-1porNature ResearchMetacyclogenesis defects and gene expression hallmarks of histone deacetylase 4‑deficient Trypanosoma cruzi cellsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Instituto Butantan. Laboratório de Parasitologia. São Paulo, SP, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Instituto Butantan. Laboratório de Parasitologia. São Paulo, SP, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Instituto Butantan. Laboratório de Parasitologia. São Paulo, SP, Brasil. / Universidade de São Paulo. Instituto de Química. Departamento de Bioquímica. São Paulo, SP, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Trypanosoma cruzi—the causative agent of Chagas disease—like other kinetoplastids, relies mostly on post-transcriptional mechanisms for regulation of gene expression. However, trypanosomatids undergo drastic changes in nuclear architecture and chromatin structure along their complex life cycle which, combined with a remarkable set of reversible histone post-translational modifcations, indicate that chromatin is also a target for control of gene expression and diferentiation signals in these organisms. Chromatin-modifying enzymes have a direct impact on gene expression programs and DNA metabolism. In this work, we have investigated the function of T. cruzi histone deacetylase 4 (TcHDAC4). We show that, although TcHDAC4 is not essential for viability, metacyclic trypomastigote TcHDAC4 null mutants show a thin cell body and a round and less condensed nucleus located very close to the kinetoplast. Sixty-four acetylation sites were quantitatively evaluated, which revealed H2AT85ac, H4K10ac and H4K78ac as potential target sites of TcHDAC4. Gene expression analyses identifed three chromosomes with overrepresented regions of diferentially expressed genes in the TcHDAC4 knockout mutant compared with the wild type, showing clusters of either up or downregulated genes. The adjacent chromosomal location of some of these genes indicates that TcHDAC4 participates in gene expression regulation during T. cruzi diferentiation.Gene Expression RegulationHistone DeacetylasesCell DifferentiationRegulación de la Expresión GénicaHistona DesacetilasasDiferenciación CelularRégulation de l'expression des gènesHistone deacetylasesDifférenciation cellulaireTrypanosoma cruziRegulação da Expressão GênicaHistona DesacetilasesDiferenciação Celularinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83084https://www.arca.fiocruz.br/bitstream/icict/50054/1/license.txt783568c2893d2e25a99990b126be1772MD51ORIGINALs41598-021-01080OK.pdfs41598-021-01080OK.pdfapplication/pdf6033169https://www.arca.fiocruz.br/bitstream/icict/50054/2/s41598-021-01080OK.pdf4516153e273e22bd9b95ca2882a288e8MD52icict/500542021-11-24 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dc.title.pt_BR.fl_str_mv Metacyclogenesis defects and gene expression hallmarks of histone deacetylase 4‑deficient Trypanosoma cruzi cells
title Metacyclogenesis defects and gene expression hallmarks of histone deacetylase 4‑deficient Trypanosoma cruzi cells
spellingShingle Metacyclogenesis defects and gene expression hallmarks of histone deacetylase 4‑deficient Trypanosoma cruzi cells
Picchi‑Constante, Gisele Fernanda Assine
Gene Expression Regulation
Histone Deacetylases
Cell Differentiation
Regulación de la Expresión Génica
Histona Desacetilasas
Diferenciación Celular
Régulation de l'expression des gènes
Histone deacetylases
Différenciation cellulaire
Trypanosoma cruzi
Regulação da Expressão Gênica
Histona Desacetilases
Diferenciação Celular
title_short Metacyclogenesis defects and gene expression hallmarks of histone deacetylase 4‑deficient Trypanosoma cruzi cells
title_full Metacyclogenesis defects and gene expression hallmarks of histone deacetylase 4‑deficient Trypanosoma cruzi cells
title_fullStr Metacyclogenesis defects and gene expression hallmarks of histone deacetylase 4‑deficient Trypanosoma cruzi cells
title_full_unstemmed Metacyclogenesis defects and gene expression hallmarks of histone deacetylase 4‑deficient Trypanosoma cruzi cells
title_sort Metacyclogenesis defects and gene expression hallmarks of histone deacetylase 4‑deficient Trypanosoma cruzi cells
author Picchi‑Constante, Gisele Fernanda Assine
author_facet Picchi‑Constante, Gisele Fernanda Assine
Guerra‑Slompo, Eloise Pavão
Tahira, Ana Carolina
Alcantara, Monica Visnieski
Amaral, Murilo Sena
Ferreira, Arthur Schveitzer
Batista, Michel
Batista, Cassiano Martin
Goldenberg, Samuel
Verjovski‑Almeida, Sergio
Zanchin, Nilson Ivo Tonin
author_role author
author2 Guerra‑Slompo, Eloise Pavão
Tahira, Ana Carolina
Alcantara, Monica Visnieski
Amaral, Murilo Sena
Ferreira, Arthur Schveitzer
Batista, Michel
Batista, Cassiano Martin
Goldenberg, Samuel
Verjovski‑Almeida, Sergio
Zanchin, Nilson Ivo Tonin
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Picchi‑Constante, Gisele Fernanda Assine
Guerra‑Slompo, Eloise Pavão
Tahira, Ana Carolina
Alcantara, Monica Visnieski
Amaral, Murilo Sena
Ferreira, Arthur Schveitzer
Batista, Michel
Batista, Cassiano Martin
Goldenberg, Samuel
Verjovski‑Almeida, Sergio
Zanchin, Nilson Ivo Tonin
dc.subject.en.pt_BR.fl_str_mv Gene Expression Regulation
Histone Deacetylases
Cell Differentiation
topic Gene Expression Regulation
Histone Deacetylases
Cell Differentiation
Regulación de la Expresión Génica
Histona Desacetilasas
Diferenciación Celular
Régulation de l'expression des gènes
Histone deacetylases
Différenciation cellulaire
Trypanosoma cruzi
Regulação da Expressão Gênica
Histona Desacetilases
Diferenciação Celular
dc.subject.es.pt_BR.fl_str_mv Regulación de la Expresión Génica
Histona Desacetilasas
Diferenciación Celular
dc.subject.fr.pt_BR.fl_str_mv Régulation de l'expression des gènes
Histone deacetylases
Différenciation cellulaire
dc.subject.decs.pt_BR.fl_str_mv Trypanosoma cruzi
Regulação da Expressão Gênica
Histona Desacetilases
Diferenciação Celular
description Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.
publishDate 2021
dc.date.accessioned.fl_str_mv 2021-11-24T19:02:33Z
dc.date.available.fl_str_mv 2021-11-24T19:02:33Z
dc.date.issued.fl_str_mv 2021
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv PICCHI-CONSTANTE, Gisele Fernanda Assine et al. Metacyclogenesis defects and gene expression hallmarks of histone deacetylase 4‑deficient Trypanosoma cruzi cells. Scientific Reports, v.11, n. 21671, p. 1–18, 2021.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/50054
dc.identifier.issn.pt_BR.fl_str_mv 2045-2322
dc.identifier.doi.none.fl_str_mv 10.1038/s41598-021-01080-1
identifier_str_mv PICCHI-CONSTANTE, Gisele Fernanda Assine et al. Metacyclogenesis defects and gene expression hallmarks of histone deacetylase 4‑deficient Trypanosoma cruzi cells. Scientific Reports, v.11, n. 21671, p. 1–18, 2021.
2045-2322
10.1038/s41598-021-01080-1
url https://www.arca.fiocruz.br/handle/icict/50054
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Nature Research
publisher.none.fl_str_mv Nature Research
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
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institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/50054/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/50054/2/s41598-021-01080OK.pdf
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
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