High Parasitic Loads Quantified in Sylvatic Triatoma melanica, a Chagas Disease Vector

Detalhes bibliográficos
Autor(a) principal: Valença-Barbosa, Carolina
Data de Publicação: 2022
Outros Autores: Finamore-Araujo, Paula, Moreira, Otacílio Cruz, Alvarez, Marcus Vinicius Niz, Borges-Veloso, André, Barbosa, Silvia Ermelinda, Diotaiuti, Liléia Gonçalves, Souza, Rita de Cássia Moreira de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/56938
Resumo: Fundação Oswaldo Cruz. Instituto René Rachou. Grupo Triatomíneos. Belo Horizonte, MG, Brazil.
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spelling Valença-Barbosa, CarolinaFinamore-Araujo, PaulaMoreira, Otacílio CruzAlvarez, Marcus Vinicius NizBorges-Veloso, AndréBarbosa, Silvia ErmelindaDiotaiuti, Liléia GonçalvesSouza, Rita de Cássia Moreira de2023-02-07T18:26:47Z2023-02-07T18:26:47Z2022VALENÇA-BARBOSA, Carolina et al. High Parasitic Loads Quantified in Sylvatic Triatoma melanica, a Chagas Disease Vector. Pathogens, v. 11, n. 12, 1498, 2022. doi: 10.3390/pathogens11121498.2076-0817https://www.arca.fiocruz.br/handle/icict/56938engMDPI AGHigh Parasitic Loads Quantified in Sylvatic Triatoma melanica, a Chagas Disease Vectorinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto René Rachou. Grupo Triatomíneos. Belo Horizonte, MG, Brazil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Molecular. Plataforma de PCR em Tempo Real RPT09A. Rio de Janeiro, RJ, Brazil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Molecular. Plataforma de PCR em Tempo Real RPT09A. Rio de Janeiro, RJ, Brazil.Universidade Estadual Paulista. Instituto de Biotecnologia de Botucatu. São Paulo, SP, Brazil.Fundação Oswaldo Cruz. Instituto René Rachou. Grupo Triatomíneos. Belo Horizonte, MG, Brazil.Fundação Oswaldo Cruz. Instituto René Rachou. Grupo Triatomíneos. Belo Horizonte, MG, Brazil.Fundação Oswaldo Cruz. Instituto René Rachou. Grupo Triatomíneos. Belo Horizonte, MG, Brazil.Fundação Oswaldo Cruz. Instituto René Rachou. Grupo Triatomíneos. Belo Horizonte, MG, Brazil.Triatoma melanica is a sylvatic vector species in Brazil. In We aimed to characterize the Trypanosoma cruzi discrete typing units (DTUs), the parasitic loads, and the blood meal sources of insects collected in rocky outcrops in rural areas in the state of Minas Gerais. An optical microscope (OM) and kDNA-PCR were used to examine natural infection by T. cruzi, and positive samples were genotyped by conventional multilocus PCR. Quantification of the T. cruzi load was performed using qPCR, and the blood meal sources were identified by Sanger sequencing the 12S rRNA gene. A total of 141 T. melanica were captured. Of these, ~55% (61/111) and ~91% (63/69) were positive by OM and KDNA-PCR, respectively. We genotyped ~89% (56/63) of the T. cruzi-positive triatomines, with TcI (~55%, 31/56) being the most prevalent DTU, followed by TcIII (~20%, 11/56) and TcII (~7%, 4/56). Only TcI+TcIII mixed infections were detected in 10 (~18%) specimens. A wide range of variation in the parasitic loads of T. melanica was observed, with an overall median value of 104 parasites/intestine, with females having higher T. cruzi loads than N2, N4, and N5. TcII showed lower parasitic loads compared to TcI and TcIII. The OM positive diagnosis odds ratio between T. cruzi infection when the parasite load is 107 compared to 103 was approximately 29.1. The most frequent blood meal source was Kerodon rupestris (~58%), followed by Thrichomys apereoides (~18%), Wiedomys cerradensis (~8%), Galactis cuja (~8%) and Gallus gallus (~8%). Our findings characterize biological and epidemiological aspects of the sylvatic population of T. melanica in the study area, highlighting the need to extend surveillance and control to this vector.Trypanosoma cruziblood-feeding behaviorbrasiliensis complexTriatominesinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALHigh Parasitic Loads Quantified in Sylvatic Triatoma melanica, a Chagas Disease Vector.pdfHigh Parasitic Loads Quantified in Sylvatic Triatoma melanica, a Chagas Disease Vector.pdfapplication/pdf8260550https://www.arca.fiocruz.br/bitstream/icict/56938/2/High%20Parasitic%20Loads%20Quantified%20in%20Sylvatic%20Triatoma%20melanica%2c%20a%20Chagas%20Disease%20Vector.pdf45779cb5932f55d5d4d6b6e06643cc24MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/56938/1/license.txt5a560609d32a3863062d77ff32785d58MD51icict/569382023-02-07 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dc.title.en_US.fl_str_mv High Parasitic Loads Quantified in Sylvatic Triatoma melanica, a Chagas Disease Vector
title High Parasitic Loads Quantified in Sylvatic Triatoma melanica, a Chagas Disease Vector
spellingShingle High Parasitic Loads Quantified in Sylvatic Triatoma melanica, a Chagas Disease Vector
Valença-Barbosa, Carolina
Trypanosoma cruzi
blood-feeding behavior
brasiliensis complex
Triatomines
title_short High Parasitic Loads Quantified in Sylvatic Triatoma melanica, a Chagas Disease Vector
title_full High Parasitic Loads Quantified in Sylvatic Triatoma melanica, a Chagas Disease Vector
title_fullStr High Parasitic Loads Quantified in Sylvatic Triatoma melanica, a Chagas Disease Vector
title_full_unstemmed High Parasitic Loads Quantified in Sylvatic Triatoma melanica, a Chagas Disease Vector
title_sort High Parasitic Loads Quantified in Sylvatic Triatoma melanica, a Chagas Disease Vector
author Valença-Barbosa, Carolina
author_facet Valença-Barbosa, Carolina
Finamore-Araujo, Paula
Moreira, Otacílio Cruz
Alvarez, Marcus Vinicius Niz
Borges-Veloso, André
Barbosa, Silvia Ermelinda
Diotaiuti, Liléia Gonçalves
Souza, Rita de Cássia Moreira de
author_role author
author2 Finamore-Araujo, Paula
Moreira, Otacílio Cruz
Alvarez, Marcus Vinicius Niz
Borges-Veloso, André
Barbosa, Silvia Ermelinda
Diotaiuti, Liléia Gonçalves
Souza, Rita de Cássia Moreira de
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Valença-Barbosa, Carolina
Finamore-Araujo, Paula
Moreira, Otacílio Cruz
Alvarez, Marcus Vinicius Niz
Borges-Veloso, André
Barbosa, Silvia Ermelinda
Diotaiuti, Liléia Gonçalves
Souza, Rita de Cássia Moreira de
dc.subject.en.en_US.fl_str_mv Trypanosoma cruzi
blood-feeding behavior
brasiliensis complex
Triatomines
topic Trypanosoma cruzi
blood-feeding behavior
brasiliensis complex
Triatomines
description Fundação Oswaldo Cruz. Instituto René Rachou. Grupo Triatomíneos. Belo Horizonte, MG, Brazil.
publishDate 2022
dc.date.issued.fl_str_mv 2022
dc.date.accessioned.fl_str_mv 2023-02-07T18:26:47Z
dc.date.available.fl_str_mv 2023-02-07T18:26:47Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv VALENÇA-BARBOSA, Carolina et al. High Parasitic Loads Quantified in Sylvatic Triatoma melanica, a Chagas Disease Vector. Pathogens, v. 11, n. 12, 1498, 2022. doi: 10.3390/pathogens11121498.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/56938
dc.identifier.issn.en_US.fl_str_mv 2076-0817
identifier_str_mv VALENÇA-BARBOSA, Carolina et al. High Parasitic Loads Quantified in Sylvatic Triatoma melanica, a Chagas Disease Vector. Pathogens, v. 11, n. 12, 1498, 2022. doi: 10.3390/pathogens11121498.
2076-0817
url https://www.arca.fiocruz.br/handle/icict/56938
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv MDPI AG
publisher.none.fl_str_mv MDPI AG
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/56938/2/High%20Parasitic%20Loads%20Quantified%20in%20Sylvatic%20Triatoma%20melanica%2c%20a%20Chagas%20Disease%20Vector.pdf
https://www.arca.fiocruz.br/bitstream/icict/56938/1/license.txt
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
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