Cell structure and cytokinesis alterations in multidrug-resistant Leishmania (Leishmania) amazonensis

Detalhes bibliográficos
Autor(a) principal: Borges, Valeria de Matos
Data de Publicação: 2004
Outros Autores: Lopes, Ulisses Gazos, Souza, Wanderley de, Santos, Marcos André Vannier dos
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/3253
Resumo: Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.
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spelling Borges, Valeria de MatosLopes, Ulisses GazosSouza, Wanderley deSantos, Marcos André Vannier dos2011-10-06T21:38:03Z2011-10-06T21:38:03Z2004BORGES, V. de M. et al. Cell structure and cytokinesis alterations in multidrug-resistant Leishmania (Leishmania) amazonensis. Parasitology Research, v.95, n.2, p.90-96, 2005.0932-0113https://www.arca.fiocruz.br/handle/icict/325310.1007/s00436-004-1248-8engCell structure and cytokinesis alterations in multidrug-resistant Leishmania (Leishmania) amazonensisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.Universidade Federal do Rio de Janeiro. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.Multidrug-resistant Leishmania (Leishmania) amazonensis may be obtained by in vitro selection with vinblastine. In order to determine whether this phenotype is linked to structural alterations, we analyzed the cell architecture by electron microscopy. The vinblastine resistant CL2 clone of L. (L.) amazonensis, but not wild-type parasites, showed a cytokinesis dysfunction. The CL2 promastigotes had multiple nuclei, kinetoplasts and flagella, suggesting that vinblastine resistance may be associated with truncated cell division. The subpellicular microtubule plasma membrane connection was also affected. Wild-type parasites treated with vinblastine displayed similar alterations, presenting lobulated and multinucleated cells. Taken together, these data indicate that antimicrotubule drug-selected parasites may show evidence of the mutation of cytoskeleton proteins, impairing normal cell function.Leishmania mexicanaResistência a Múltiplos MedicamentosMicroscopia EletrônicaMicroscopia Eletrônica de VarreduraVimblastinaCitoesqueletoCitocineseAnimaisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALBorges VM Cell structure and cytokinesis....pdfBorges VM Cell structure and cytokinesis....pdfapplication/pdf485360https://www.arca.fiocruz.br/bitstream/icict/3253/1/Borges%20VM%20Cell%20structure%20and%20cytokinesis....pdf5a5defb4a74c652e77edfd8184374a9fMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81648https://www.arca.fiocruz.br/bitstream/icict/3253/2/license.txte095249ac7cacefbfe39684dfe45e706MD52TEXTBorges VM Cell structure and cytokinesis....pdf.txtBorges VM Cell structure and cytokinesis....pdf.txtExtracted texttext/plain29154https://www.arca.fiocruz.br/bitstream/icict/3253/5/Borges%20VM%20Cell%20structure%20and%20cytokinesis....pdf.txt831d0217f9d97ed24dbc635b9a06ca8aMD55THUMBNAILBorges VM Cell structure and cytokinesis....pdf.jpgBorges VM Cell structure and cytokinesis....pdf.jpgGenerated Thumbnailimage/jpeg2296https://www.arca.fiocruz.br/bitstream/icict/3253/4/Borges%20VM%20Cell%20structure%20and%20cytokinesis....pdf.jpg715def54dfa0196dc5a83906cf1d295bMD54icict/32532023-03-15 14:32:56.013oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:32:56Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Cell structure and cytokinesis alterations in multidrug-resistant Leishmania (Leishmania) amazonensis
title Cell structure and cytokinesis alterations in multidrug-resistant Leishmania (Leishmania) amazonensis
spellingShingle Cell structure and cytokinesis alterations in multidrug-resistant Leishmania (Leishmania) amazonensis
Borges, Valeria de Matos
Leishmania mexicana
Resistência a Múltiplos Medicamentos
Microscopia Eletrônica
Microscopia Eletrônica de Varredura
Vimblastina
Citoesqueleto
Citocinese
Animais
title_short Cell structure and cytokinesis alterations in multidrug-resistant Leishmania (Leishmania) amazonensis
title_full Cell structure and cytokinesis alterations in multidrug-resistant Leishmania (Leishmania) amazonensis
title_fullStr Cell structure and cytokinesis alterations in multidrug-resistant Leishmania (Leishmania) amazonensis
title_full_unstemmed Cell structure and cytokinesis alterations in multidrug-resistant Leishmania (Leishmania) amazonensis
title_sort Cell structure and cytokinesis alterations in multidrug-resistant Leishmania (Leishmania) amazonensis
author Borges, Valeria de Matos
author_facet Borges, Valeria de Matos
Lopes, Ulisses Gazos
Souza, Wanderley de
Santos, Marcos André Vannier dos
author_role author
author2 Lopes, Ulisses Gazos
Souza, Wanderley de
Santos, Marcos André Vannier dos
author2_role author
author
author
dc.contributor.author.fl_str_mv Borges, Valeria de Matos
Lopes, Ulisses Gazos
Souza, Wanderley de
Santos, Marcos André Vannier dos
dc.subject.decs.pt_BR.fl_str_mv Leishmania mexicana
Resistência a Múltiplos Medicamentos
Microscopia Eletrônica
Microscopia Eletrônica de Varredura
Vimblastina
Citoesqueleto
Citocinese
Animais
topic Leishmania mexicana
Resistência a Múltiplos Medicamentos
Microscopia Eletrônica
Microscopia Eletrônica de Varredura
Vimblastina
Citoesqueleto
Citocinese
Animais
description Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.
publishDate 2004
dc.date.issued.fl_str_mv 2004
dc.date.accessioned.fl_str_mv 2011-10-06T21:38:03Z
dc.date.available.fl_str_mv 2011-10-06T21:38:03Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv BORGES, V. de M. et al. Cell structure and cytokinesis alterations in multidrug-resistant Leishmania (Leishmania) amazonensis. Parasitology Research, v.95, n.2, p.90-96, 2005.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/3253
dc.identifier.issn.none.fl_str_mv 0932-0113
dc.identifier.doi.none.fl_str_mv 10.1007/s00436-004-1248-8
identifier_str_mv BORGES, V. de M. et al. Cell structure and cytokinesis alterations in multidrug-resistant Leishmania (Leishmania) amazonensis. Parasitology Research, v.95, n.2, p.90-96, 2005.
0932-0113
10.1007/s00436-004-1248-8
url https://www.arca.fiocruz.br/handle/icict/3253
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