Different serological cross-reactivity of Trypanosoma rangeli forms in Trypanosoma cruzi-infected patients sera

Detalhes bibliográficos
Autor(a) principal: Moraes, Milene H de
Data de Publicação: 2008
Outros Autores: Guarneri, Alessandra Aparecida, Girardi, Fabiana P, Rodrigues, Juliana B, Eger, Iriane, Tyler, Kevin Morris, Steindel, Mário, Grisard, Edmundo Carlos
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/54333
Resumo: CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO
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spelling Moraes, Milene H deGuarneri, Alessandra AparecidaGirardi, Fabiana PRodrigues, Juliana BEger, IrianeTyler, Kevin MorrisSteindel, MárioGrisard, Edmundo Carlos2022-08-02T18:44:42Z2022-08-02T18:44:42Z2008MORAES, Milene H de et al. Different serological cross-reactivity of Trypanosoma rangeli forms in Trypanosoma cruzi-infected patients sera. Parasites Vectors, v. 1, n. 1, 20, 2008. doi.org/10.1186/1756-3305-1-201756-3305https://www.arca.fiocruz.br/handle/icict/5433310.1186/1756-3305-1-20CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICOUniversidade Federal de Santa Catarina. Departamento de Microbiologia e Parasitologia. Florianópolis, SC, BrazilUniversidade Federal de Santa Catarina. Departamento de Microbiologia e Parasitologia. Florianópolis, SC, Brazil/Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, BrazilUniversidade Federal de Santa Catarina. Departamento de Microbiologia e Parasitologia. Florianópolis, SC, BrazilUniversidade Federal de Santa Catarina. Departamento de Microbiologia e Parasitologia. Florianópolis, SC, BrazilUniversidade Federal de Santa Catarina. Departamento de Microbiologia e Parasitologia. Florianópolis, SC, Brazil/Universidade do Vale do Itajaí. Itajaí, SC, BrazilBiomedical Research Centre. School of Medicine, Health Policy and Practice. University of East Anglia. Norwich, Norfolk, UKUniversidade Federal de Santa Catarina. Departamento de Microbiologia e Parasitologia. Florianópolis, SC, BrazilUniversidade Federal de Santa Catarina. Departamento de Microbiologia e Parasitologia. Florianópolis, SC, Brazil/Universidade do Vale do Itajaí. Itajaí, SC, Brazil/Biomedical Research Centre. School of Medicine, Health Policy and Practice. University of East Anglia. Norwich, Norfolk, UKBackground: American Trypanosomiasis or Chagas disease is caused by Trypanosoma cruzi which currently infects approximately 16 million people in the Americas causing high morbidity and mortality. Diagnosis of American trypanosomiasis relies on serology, primarily using indirect immunofluorescence assay (IFA) with T. cruzi epimastigote forms. The closely related but nonpathogenic Trypanosoma rangeli has a sympatric distribution with T. cruzi and is carried by the same vectors. As a result false positives are frequently generated. This confounding factor leads to increased diagnostic test costs and where false positives are not caught, endangers human health due to the toxicity of the drugs used to treat Chagas disease. Results: In the present study, serologic cross-reactivity between the two species was compared for the currently used epimastigote form and the more pathologically relevant trypomastigote form, using IFA and immunoblotting (IB) assays. Our results reveal an important decrease in cross reactivity when T. rangeli culture-derived trypomastigotes are used in IFA based diagnosis of Chagas disease. Western blot results using sera from both acute and chronic chagasic patients presenting with cardiac, indeterminate or digestive disease revealed similar, but not identical, antigenic profiles. Conclusion: This is the first study addressing the serological cross-reactivity between distinct forms and strains of T. rangeli and T. cruzi using sera from distinct phases of the Chagasic infection. Several T. rangeli-specific proteins were detected, which may have potential as diagnostic toolsengBioMed CentralDifferent serological cross-reactivity of Trypanosoma rangeli forms in Trypanosoma cruzi-infected patients serainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALDifferent serological cross-reactivity of Trypanosoma rangeli.pdfDifferent serological cross-reactivity of Trypanosoma rangeli.pdfapplication/pdf449481https://www.arca.fiocruz.br/bitstream/icict/54333/2/Different%20serological%20cross-reactivity%20of%20Trypanosoma%20rangeli.pdf599c1203b61b2864c3227f10b4ba2cb1MD52LICENSElicense.txttext/plain1748https://www.arca.fiocruz.br/bitstream/icict/54333/1/license.txt8a4605be74aa9ea9d79846c1fba20a33MD51icict/543332022-08-02 15:44:42.132oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352022-08-02T18:44:42Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Different serological cross-reactivity of Trypanosoma rangeli forms in Trypanosoma cruzi-infected patients sera
title Different serological cross-reactivity of Trypanosoma rangeli forms in Trypanosoma cruzi-infected patients sera
spellingShingle Different serological cross-reactivity of Trypanosoma rangeli forms in Trypanosoma cruzi-infected patients sera
Moraes, Milene H de
title_short Different serological cross-reactivity of Trypanosoma rangeli forms in Trypanosoma cruzi-infected patients sera
title_full Different serological cross-reactivity of Trypanosoma rangeli forms in Trypanosoma cruzi-infected patients sera
title_fullStr Different serological cross-reactivity of Trypanosoma rangeli forms in Trypanosoma cruzi-infected patients sera
title_full_unstemmed Different serological cross-reactivity of Trypanosoma rangeli forms in Trypanosoma cruzi-infected patients sera
title_sort Different serological cross-reactivity of Trypanosoma rangeli forms in Trypanosoma cruzi-infected patients sera
author Moraes, Milene H de
author_facet Moraes, Milene H de
Guarneri, Alessandra Aparecida
Girardi, Fabiana P
Rodrigues, Juliana B
Eger, Iriane
Tyler, Kevin Morris
Steindel, Mário
Grisard, Edmundo Carlos
author_role author
author2 Guarneri, Alessandra Aparecida
Girardi, Fabiana P
Rodrigues, Juliana B
Eger, Iriane
Tyler, Kevin Morris
Steindel, Mário
Grisard, Edmundo Carlos
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Moraes, Milene H de
Guarneri, Alessandra Aparecida
Girardi, Fabiana P
Rodrigues, Juliana B
Eger, Iriane
Tyler, Kevin Morris
Steindel, Mário
Grisard, Edmundo Carlos
description CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO
publishDate 2008
dc.date.issued.fl_str_mv 2008
dc.date.accessioned.fl_str_mv 2022-08-02T18:44:42Z
dc.date.available.fl_str_mv 2022-08-02T18:44:42Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv MORAES, Milene H de et al. Different serological cross-reactivity of Trypanosoma rangeli forms in Trypanosoma cruzi-infected patients sera. Parasites Vectors, v. 1, n. 1, 20, 2008. doi.org/10.1186/1756-3305-1-20
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/54333
dc.identifier.issn.pt_BR.fl_str_mv 1756-3305
dc.identifier.doi.none.fl_str_mv 10.1186/1756-3305-1-20
identifier_str_mv MORAES, Milene H de et al. Different serological cross-reactivity of Trypanosoma rangeli forms in Trypanosoma cruzi-infected patients sera. Parasites Vectors, v. 1, n. 1, 20, 2008. doi.org/10.1186/1756-3305-1-20
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