Antigenicity of cystatin-binding proteins from parasitic protozoan. Detection by a proteinase inhibitor based capture immunoassay (PINC-ELISA)

Detalhes bibliográficos
Autor(a) principal: Scharfstein, Julio
Data de Publicação: 1995
Outros Autores: Abrahamson, M., Souza, Clarissa Beatriz Palatnik de, Barral, Aldina Maria Prado, Silva, I. V.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/22856
Resumo: Barral, Aldina Maria Prado. “Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento”.
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spelling Scharfstein, JulioAbrahamson, M.Souza, Clarissa Beatriz Palatnik deBarral, Aldina Maria PradoSilva, I. V.2017-10-17T18:09:11Z2017-10-17T18:09:11Z1995SCHARFSTEIN, J. et al. Antigenicity of cystatin-binding proteins from parasitic protozoan. Detection by a proteinase inhibitor based capture immunoassay (PINC-ELISA). Journal of Immunological Methods, v. 182, p. 63-72, 1995.0022-1759https://www.arca.fiocruz.br/handle/icict/22856Barral, Aldina Maria Prado. “Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento”.European Community (EEC), FINEP, CNPq, and CEPG-UFRJ.Instituto de Biofísica Carlos Chagas Filho. Laboratory of Molecular Immunology. Rio de Janeiro, RJ, Brasil.University of Lund. Department of Clinical Chemistry. Lund, Sweden.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia. Rio de Janeiro, RJ, Brasil.Universidade Federal da Bahia. Hospital Universitario Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil.Instituto de Biofísica Carlos Chagas Filho. Laboratory of Molecular Immunology. Rio de Janeiro, RJ, Brasil.A novel immunoassay (PINC-ELISA) was designed using proteinase inhibitors of the cystatin superfamily (PINC) in the solid phase, to promote the selective capture of cysteine proteinases. The method was applied in the identification of papain-like antigens from parasitic protozoa. PINC of human origin, namely recombinant cystatin C (r-cystatin C) or low molecular weight kininogen were used in the assays to adsorb proteases contained in cell lysates from various trypanosomatids. The PINC-ELISA was at first optimized with the major cysteine proteinase from Trypanosoma cruzi (known as GP57/51 or cruzipain), an antigen whose serodiagnostic properties were previously established. Cruzipain is selectively adsorbed from crude extracts of T. cruzi onto PINC-coated wells; the finding that antibodies bind to epitopes located away from the sites of interaction with r-cystatin or low molecular weight kininogen has allowed for the screening of antibodies in chagasic sera, the methodology being advantageous in that it dispensed prior purification of the proteinase antigen. The PINC-ELISA was then carried out with lysates originating from Leishmania m. amazonensis (amastigotes) or Leishmania donovani (promastigotes). Complexes between solid-phase r-cystatin C and antigenic ligands in the lysates were again detected. The Leishmania molecules which bound to r-cystatin C, were respectively recognized by serum antibodies from mice chronically infected with L. amazonensis or from patients with visceral leishmaniasis. Direct evidence for the presence of cysteine proteinases in lysates from L. donovani was then obtained, using synthetic fluorogenic substrates.(ABSTRACT TRUNCATED AT 250 WORDS)engElsevierTrypanosoma cruziLeishmaniaAnticorpos monoclonaisTestes de precipitinaAnimaisRatosTrypanosoma cruziLeishmaniaAntibodies, MonoclonalPrecipitin TestsAnimalsMiceAntigenicity of cystatin-binding proteins from parasitic protozoan. Detection by a proteinase inhibitor based capture immunoassay (PINC-ELISA)info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/22856/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALScharfstein J Antigenicity....pdfScharfstein J Antigenicity....pdfapplication/pdf999028https://www.arca.fiocruz.br/bitstream/icict/22856/2/Scharfstein%20J%20Antigenicity....pdf0517c0b32a8bbb053e47114885149a4dMD52TEXTScharfstein J Antigenicity....pdf.txtScharfstein J Antigenicity....pdf.txtExtracted texttext/plain36678https://www.arca.fiocruz.br/bitstream/icict/22856/3/Scharfstein%20J%20Antigenicity....pdf.txt936174a0f200ce73de9f1ee3af12e1ebMD53icict/228562023-03-15 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dc.title.pt_BR.fl_str_mv Antigenicity of cystatin-binding proteins from parasitic protozoan. Detection by a proteinase inhibitor based capture immunoassay (PINC-ELISA)
title Antigenicity of cystatin-binding proteins from parasitic protozoan. Detection by a proteinase inhibitor based capture immunoassay (PINC-ELISA)
spellingShingle Antigenicity of cystatin-binding proteins from parasitic protozoan. Detection by a proteinase inhibitor based capture immunoassay (PINC-ELISA)
Scharfstein, Julio
Trypanosoma cruzi
Leishmania
Anticorpos monoclonais
Testes de precipitina
Animais
Ratos
Trypanosoma cruzi
Leishmania
Antibodies, Monoclonal
Precipitin Tests
Animals
Mice
title_short Antigenicity of cystatin-binding proteins from parasitic protozoan. Detection by a proteinase inhibitor based capture immunoassay (PINC-ELISA)
title_full Antigenicity of cystatin-binding proteins from parasitic protozoan. Detection by a proteinase inhibitor based capture immunoassay (PINC-ELISA)
title_fullStr Antigenicity of cystatin-binding proteins from parasitic protozoan. Detection by a proteinase inhibitor based capture immunoassay (PINC-ELISA)
title_full_unstemmed Antigenicity of cystatin-binding proteins from parasitic protozoan. Detection by a proteinase inhibitor based capture immunoassay (PINC-ELISA)
title_sort Antigenicity of cystatin-binding proteins from parasitic protozoan. Detection by a proteinase inhibitor based capture immunoassay (PINC-ELISA)
author Scharfstein, Julio
author_facet Scharfstein, Julio
Abrahamson, M.
Souza, Clarissa Beatriz Palatnik de
Barral, Aldina Maria Prado
Silva, I. V.
author_role author
author2 Abrahamson, M.
Souza, Clarissa Beatriz Palatnik de
Barral, Aldina Maria Prado
Silva, I. V.
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Scharfstein, Julio
Abrahamson, M.
Souza, Clarissa Beatriz Palatnik de
Barral, Aldina Maria Prado
Silva, I. V.
dc.subject.other.pt_BR.fl_str_mv Trypanosoma cruzi
Leishmania
Anticorpos monoclonais
Testes de precipitina
Animais
Ratos
topic Trypanosoma cruzi
Leishmania
Anticorpos monoclonais
Testes de precipitina
Animais
Ratos
Trypanosoma cruzi
Leishmania
Antibodies, Monoclonal
Precipitin Tests
Animals
Mice
dc.subject.en.pt_BR.fl_str_mv Trypanosoma cruzi
Leishmania
Antibodies, Monoclonal
Precipitin Tests
Animals
Mice
description Barral, Aldina Maria Prado. “Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento”.
publishDate 1995
dc.date.issued.fl_str_mv 1995
dc.date.accessioned.fl_str_mv 2017-10-17T18:09:11Z
dc.date.available.fl_str_mv 2017-10-17T18:09:11Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SCHARFSTEIN, J. et al. Antigenicity of cystatin-binding proteins from parasitic protozoan. Detection by a proteinase inhibitor based capture immunoassay (PINC-ELISA). Journal of Immunological Methods, v. 182, p. 63-72, 1995.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/22856
dc.identifier.issn.pt_BR.fl_str_mv 0022-1759
identifier_str_mv SCHARFSTEIN, J. et al. Antigenicity of cystatin-binding proteins from parasitic protozoan. Detection by a proteinase inhibitor based capture immunoassay (PINC-ELISA). Journal of Immunological Methods, v. 182, p. 63-72, 1995.
0022-1759
url https://www.arca.fiocruz.br/handle/icict/22856
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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