Thermosensitive system formed by poloxamers containing carvacrol: An effective carrier system against Leishmania amazonensis
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2023 |
| Outros Autores: | , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/58190 |
Resumo: | 2030 |
| id |
CRUZ_a9455d912b284e883b9ea0244680b672 |
|---|---|
| oai_identifier_str |
oai:www.arca.fiocruz.br:icict/58190 |
| network_acronym_str |
CRUZ |
| network_name_str |
Repositório Institucional da FIOCRUZ (ARCA) |
| repository_id_str |
2135 |
| spelling |
Costa, Amanda Mendonça BarrosSilva, Audrey Roouse Soares TavaresSantos, Adriana de JesusGalvão, Juliana GouveiaAndrade Neto, Valter VianaSantos, Eduardo Caio TorresUeki, Marcelo MassayoshiAlmeida, Luis EduardoSarmento, Victor Hugo VitorinoDolabella, Silvio SantanaScher, RicardoLira, Ana Amélia MoreiraNunes, Rogéria de Souza2023-05-06T19:10:20Z2023-05-06T19:10:20Z2023COSTA, Amanda Mendonça Barros et al. Acta Tropica, v. 237, p. 1-11, 106744, 2023.0001-706Xhttps://www.arca.fiocruz.br/handle/icict/5819010.1016/j.actatropica.2022.106744engElsevier B.V.PolímeroHidrogelSistema de entrega de drogasCitotoxicidadeLeishmanioseMonoterpenoPolymerHydrogelDrug delivery systemCytotoxicityLeishmaniasisMonoterpeneThermosensitive system formed by poloxamers containing carvacrol: An effective carrier system against Leishmania amazonensisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2030Universidade Federal de Sergipe. Departamento de Farmácia. São Cristóvão, SE, Brasil.Universidade Federal de Sergipe. Departamento de Farmácia. São Cristóvão, SE, Brasil.Universidade Federal de Sergipe. Departamento de Farmácia. São Cristóvão, SE, Brasil.Universidade Federal de Sergipe. Departamento de Farmácia. São Cristóvão, SE, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica de Tripanossomatídeos. Rio de janeiro, RJ, Brasil. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica de Tripanossomatídeos. Rio de janeiro, RJ, Brasil.Universidade Federal de Sergipe. Departamento de Ciência e Engenharia de Materiais. São Cristóvão, SE, Brasil.Universidade Federal de Sergipe. Departamento de Ciência e Engenharia de Materiais. São Cristóvão, SE, Brasil.Universidade Federal de Sergipe. Departamento de Química, Itabaiana, SE, Brasil.Universidade Federal de Sergipe. Departamento de Farmácia. São Cristóvão, SE, Brasil / Universidade Federal de Sergipe. Departamento de Morfologia. São Cristóvão, SE, Brasil.Universidade Federal de Sergipe. Departamento de Morfologia. São Cristóvão, SE, Brasil.Universidade Federal de Sergipe. Departamento de Farmácia. São Cristóvão, SE, Brasil.Universidade Federal de Sergipe. Departamento de Farmácia. São Cristóvão, SE, Brasil.The drugs used in the treatment of leishmaniasis show problems concerning side effects and toxicity. As a result, the search for new actives is necessary, and natural products like carvacrol - 5-isopropyl-2-methylphenol, become a relevant alternative. To enable the use of carvacrol as an antileishmanial agent, thermosensitive hydrogels were developed from poloxamer triblock copolymers 407 (P407) and 188 (P188). Carvacrol-free and carvacrolcontaining hydrogels were obtained from P407 alone and from the mixture of P407 and P188. The hydrogels were subjected to Differential scanning calorimetry, Small-angle X-ray scattering, Scanning electron microscopy, and Rheology analysis. The activity of hydrogels and carvacrol isolated against promastigotes and intracellular amastigotes of Leishmania amazonensis and their cytotoxicity in mammalian cells was determined. The sol-gel transition temperature for the binary hydrogel containing carvacrol (HG407/188CA) was 37.04 ± 1.35 ◦C. HG407/188CA presented lamellar structure at temperatures of 25 ◦C and 37 ◦C. HG407/188CA and carvacrol presented IC50 against Leishmania amazonensis promastigotes of 18.68 ± 1.43 μg/mL and 23.83 ± 3.32 μg/mL, respectively, and IC50 against Leishmania amazonensis amastigotes of 35.08 ± 0.75 μg/mL and 29.32 ± 0.21 μg/ mL, respectively. HG407/188CA reduced the toxicity of carvacrol in all mammalian cells evaluated, raising the CC50 in murine peritoneal macrophages from 40.23 ± 0.21 μg/mL to 332.6 ± 4.89 μg/mL, obtaining a Selectivity Index (SI) of 9.5 against 1.37 of the isolated carvacrol. HG407/188CA provided higher selectivity of carvacrol for the parasite. Thus, the binary hydrogel obtained may enable the use of carvacrol as a potential antileishmanial agent.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/58190/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALEduardoCaioTorrres_ValterANeto_et-al_IOC_2023.pdfEduardoCaioTorrres_ValterANeto_et-al_IOC_2023.pdfapplication/pdf2682184https://www.arca.fiocruz.br/bitstream/icict/58190/2/EduardoCaioTorrres_ValterANeto_et-al_IOC_2023.pdfe3de7f68ac9250c68ae7ae26c28fcde6MD52icict/581902023-09-04 10:37:09.425oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-09-04T13:37:09Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.en_US.fl_str_mv |
Thermosensitive system formed by poloxamers containing carvacrol: An effective carrier system against Leishmania amazonensis |
| title |
Thermosensitive system formed by poloxamers containing carvacrol: An effective carrier system against Leishmania amazonensis |
| spellingShingle |
Thermosensitive system formed by poloxamers containing carvacrol: An effective carrier system against Leishmania amazonensis Costa, Amanda Mendonça Barros Polímero Hidrogel Sistema de entrega de drogas Citotoxicidade Leishmaniose Monoterpeno Polymer Hydrogel Drug delivery system Cytotoxicity Leishmaniasis Monoterpene |
| title_short |
Thermosensitive system formed by poloxamers containing carvacrol: An effective carrier system against Leishmania amazonensis |
| title_full |
Thermosensitive system formed by poloxamers containing carvacrol: An effective carrier system against Leishmania amazonensis |
| title_fullStr |
Thermosensitive system formed by poloxamers containing carvacrol: An effective carrier system against Leishmania amazonensis |
| title_full_unstemmed |
Thermosensitive system formed by poloxamers containing carvacrol: An effective carrier system against Leishmania amazonensis |
| title_sort |
Thermosensitive system formed by poloxamers containing carvacrol: An effective carrier system against Leishmania amazonensis |
| author |
Costa, Amanda Mendonça Barros |
| author_facet |
Costa, Amanda Mendonça Barros Silva, Audrey Roouse Soares Tavares Santos, Adriana de Jesus Galvão, Juliana Gouveia Andrade Neto, Valter Viana Santos, Eduardo Caio Torres Ueki, Marcelo Massayoshi Almeida, Luis Eduardo Sarmento, Victor Hugo Vitorino Dolabella, Silvio Santana Scher, Ricardo Lira, Ana Amélia Moreira Nunes, Rogéria de Souza |
| author_role |
author |
| author2 |
Silva, Audrey Roouse Soares Tavares Santos, Adriana de Jesus Galvão, Juliana Gouveia Andrade Neto, Valter Viana Santos, Eduardo Caio Torres Ueki, Marcelo Massayoshi Almeida, Luis Eduardo Sarmento, Victor Hugo Vitorino Dolabella, Silvio Santana Scher, Ricardo Lira, Ana Amélia Moreira Nunes, Rogéria de Souza |
| author2_role |
author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Costa, Amanda Mendonça Barros Silva, Audrey Roouse Soares Tavares Santos, Adriana de Jesus Galvão, Juliana Gouveia Andrade Neto, Valter Viana Santos, Eduardo Caio Torres Ueki, Marcelo Massayoshi Almeida, Luis Eduardo Sarmento, Victor Hugo Vitorino Dolabella, Silvio Santana Scher, Ricardo Lira, Ana Amélia Moreira Nunes, Rogéria de Souza |
| dc.subject.other.en_US.fl_str_mv |
Polímero Hidrogel Sistema de entrega de drogas Citotoxicidade Leishmaniose Monoterpeno |
| topic |
Polímero Hidrogel Sistema de entrega de drogas Citotoxicidade Leishmaniose Monoterpeno Polymer Hydrogel Drug delivery system Cytotoxicity Leishmaniasis Monoterpene |
| dc.subject.en.en_US.fl_str_mv |
Polymer Hydrogel Drug delivery system Cytotoxicity Leishmaniasis Monoterpene |
| description |
2030 |
| publishDate |
2023 |
| dc.date.accessioned.fl_str_mv |
2023-05-06T19:10:20Z |
| dc.date.available.fl_str_mv |
2023-05-06T19:10:20Z |
| dc.date.issued.fl_str_mv |
2023 |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.citation.fl_str_mv |
COSTA, Amanda Mendonça Barros et al. Acta Tropica, v. 237, p. 1-11, 106744, 2023. |
| dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/58190 |
| dc.identifier.issn.en_US.fl_str_mv |
0001-706X |
| dc.identifier.eissn.none.fl_str_mv |
10.1016/j.actatropica.2022.106744 |
| identifier_str_mv |
COSTA, Amanda Mendonça Barros et al. Acta Tropica, v. 237, p. 1-11, 106744, 2023. 0001-706X 10.1016/j.actatropica.2022.106744 |
| url |
https://www.arca.fiocruz.br/handle/icict/58190 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.publisher.none.fl_str_mv |
Elsevier B.V. |
| publisher.none.fl_str_mv |
Elsevier B.V. |
| dc.source.none.fl_str_mv |
reponame:Repositório Institucional da FIOCRUZ (ARCA) instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ |
| instname_str |
Fundação Oswaldo Cruz (FIOCRUZ) |
| instacron_str |
FIOCRUZ |
| institution |
FIOCRUZ |
| reponame_str |
Repositório Institucional da FIOCRUZ (ARCA) |
| collection |
Repositório Institucional da FIOCRUZ (ARCA) |
| bitstream.url.fl_str_mv |
https://www.arca.fiocruz.br/bitstream/icict/58190/1/license.txt https://www.arca.fiocruz.br/bitstream/icict/58190/2/EduardoCaioTorrres_ValterANeto_et-al_IOC_2023.pdf |
| bitstream.checksum.fl_str_mv |
5a560609d32a3863062d77ff32785d58 e3de7f68ac9250c68ae7ae26c28fcde6 |
| bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 |
| repository.name.fl_str_mv |
Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ) |
| repository.mail.fl_str_mv |
repositorio.arca@fiocruz.br |
| _version_ |
1813008954824851456 |