Schistosoma mansoni Stomatin Like Protein-2 Is Located in the Tegument and Induces Partial Protection against Challenge Infection

Detalhes bibliográficos
Autor(a) principal: Farias, Leonardo P.
Data de Publicação: 2010
Outros Autores: Cardoso, Fernanda C., Miyasato, Patricia A., Montoya, Bogar O., Tararam, Cibele A., Roffato, Henrique K., Kawano, Toshie, Gazzinelli, Andréa Pereira, Oliveira, Rodrigo Corrêa de, Coulson, Patricia S., Wilson, R. Alan, Oliveira, Sérgio Costa, Leite, Luciana C. C.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/5584
Resumo: Instituto Butantan. Centro de Biotecnologia. Sao Paulo, SP, Brazil
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spelling Farias, Leonardo P.Cardoso, Fernanda C.Miyasato, Patricia A.Montoya, Bogar O.Tararam, Cibele A.Roffato, Henrique K.Kawano, ToshieGazzinelli, Andréa PereiraOliveira, Rodrigo Corrêa deCoulson, Patricia S.Wilson, R. AlanOliveira, Sérgio CostaLeite, Luciana C. C.2012-09-27T16:30:55Z2012-09-27T16:30:55Z2010FARIAS, Leonardo P et al. Schistosoma mansoni Stomatin Like Protein-2 Is Located in the Tegument and Induces Partial Protection against Challenge Infection. PLoS Negl Trop Dis. 2010 Feb 9;4(2):e597.19352727https://www.arca.fiocruz.br/handle/icict/558410.1371/journal.pntd.0000597engPublic Library of ScienceFarias LP, Cardoso FC, Miyasato PA, Montoya BO, Tararam CA, et al. (2010) Schistosoma mansoni Stomatin Like Protein-2 Is Located in the Tegument and Induces Partial Protection against Challenge Infection. PLoS Negl Trop Dis 4(2): e597Schistosoma mansoni Stomatin Like Protein-2 Is Located in the Tegument and Induces Partial Protection against Challenge Infectioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleInstituto Butantan. Centro de Biotecnologia. Sao Paulo, SP, BrazilUniversidade Federal de Minas Gerais. , Instituto de Ciencias Biologicas. Departamento de Bioquımica e Imunologia. Belo Horizonte, MG, BrazilInstituto Butantan. Centro de Biotecnologia. Sao Paulo, SP, BrazilInstituto Butantan. Centro de Biotecnologia. Sao Paulo, SP, BrazilInstituto Butantan. Centro de Biotecnologia. Sao Paulo, SP, BrazilInstituto Butantan. Centro de Biotecnologia. Sao Paulo, SP, BrazilInstituto Butantan. Centro de Biotecnologia. Sao Paulo, SP, BrazilUniversidade Federal de Minas Gerais. Escola de Enfermagem. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratorio de Imunologia e Biologia Molecular. Belo Horizonte, MG, BrazilUniversity of York. Department of Biology. York, United KingdomUniversity of York. Department of Biology. York, United KingdomUniversidade Federal de Minas Gerais. , Instituto de Ciencias Biologicas. Departamento de Bioquımica e Imunologia. Belo Horizonte, MG, BrazilInstituto Butantan. Centro de Biotecnologia. Sao Paulo, SP, BrazilBackground: Schistosomiasis affects more than 200 million individuals worldwide, with a further 650 million living at risk of infection, constituting a severe health problem in developing countries. Even though an effective treatment exists, it does not prevent re-infection, and the development of an effective vaccine still remains the most desirable means of control for this disease. Methodology/Principal Findings: Herein, we report the cloning and characterization of a S. mansoni Stomatin-like protein 2 (SmStoLP-2). In silico analysis predicts three putative sites for palmitoylation (Cys11, Cys61 and Cys330), which could contribute to protein membrane association; and a putative mitochondrial targeting sequence, similar to that described for human Stomatin-like protein 2 (HuSLP-2). The protein was detected by Western blot with comparable levels in all stages across the parasite life cycle. Fractionation by differential centrifugation of schistosome tegument suggested that SmStoLP-2 displays a dual targeting to the tegument membranes and mitochondria; additionally, immunolocalization experiments confirm its localization in the tegument of the adult worms and, more importantly, in 7-day-old schistosomula. Analysis of the antibody isotype profile to rSmStoLP-2 in the sera of patients living in endemic areas for schistosomiasis revealed that IgG1, IgG2, IgG3 and IgA antibodies were predominant in sera of individuals resistant to reinfection as compared to those susceptible. Next, immunization of mice with rSmStoLP-2 engendered a 30%–32% reduction in adult worm burden. Protective immunity in mice was associated with specific anti-rSmStoLP-2 IgG1 and IgG2a antibodies and elevated production of IFN-c and TNF-a, while no IL-4 production was detected, suggesting a Th1-predominant immune response. Conclusions/Significance: Data presented here demonstrate that SmStoLP-2 is a novel tegument protein located in the host-parasite interface. It is recognized by different subclasses of antibodies in patients resistant and susceptible to reinfection and, based on the data from murine studies, shows protective potential against schistosomiasis. These results indicate that SmStoLP-2 could be useful in a combination vaccine.SchistosomiasisSchistosoma mansoniinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINAL38.2010.pdf38.2010.pdfapplication/pdf1632791https://www.arca.fiocruz.br/bitstream/icict/5584/1/38.2010.pdf030b6db40b516a0bad66b4f1f4966b6aMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-82008https://www.arca.fiocruz.br/bitstream/icict/5584/2/license.txt06db3f0df647fd9ddff657403bbe0ee2MD52TEXT38.2010.pdf.txt38.2010.pdf.txtExtracted texttext/plain73161https://www.arca.fiocruz.br/bitstream/icict/5584/5/38.2010.pdf.txtd8c87ca577ec016d4378b500aef2794dMD55THUMBNAIL38.2010.pdf.jpg38.2010.pdf.jpgGenerated Thumbnailimage/jpeg2008https://www.arca.fiocruz.br/bitstream/icict/5584/4/38.2010.pdf.jpgffcc000e4be169ce22fa7facc9967820MD54icict/55842018-04-06 14:48:13.425oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352018-04-06T17:48:13Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Schistosoma mansoni Stomatin Like Protein-2 Is Located in the Tegument and Induces Partial Protection against Challenge Infection
title Schistosoma mansoni Stomatin Like Protein-2 Is Located in the Tegument and Induces Partial Protection against Challenge Infection
spellingShingle Schistosoma mansoni Stomatin Like Protein-2 Is Located in the Tegument and Induces Partial Protection against Challenge Infection
Farias, Leonardo P.
Schistosomiasis
Schistosoma mansoni
title_short Schistosoma mansoni Stomatin Like Protein-2 Is Located in the Tegument and Induces Partial Protection against Challenge Infection
title_full Schistosoma mansoni Stomatin Like Protein-2 Is Located in the Tegument and Induces Partial Protection against Challenge Infection
title_fullStr Schistosoma mansoni Stomatin Like Protein-2 Is Located in the Tegument and Induces Partial Protection against Challenge Infection
title_full_unstemmed Schistosoma mansoni Stomatin Like Protein-2 Is Located in the Tegument and Induces Partial Protection against Challenge Infection
title_sort Schistosoma mansoni Stomatin Like Protein-2 Is Located in the Tegument and Induces Partial Protection against Challenge Infection
author Farias, Leonardo P.
author_facet Farias, Leonardo P.
Cardoso, Fernanda C.
Miyasato, Patricia A.
Montoya, Bogar O.
Tararam, Cibele A.
Roffato, Henrique K.
Kawano, Toshie
Gazzinelli, Andréa Pereira
Oliveira, Rodrigo Corrêa de
Coulson, Patricia S.
Wilson, R. Alan
Oliveira, Sérgio Costa
Leite, Luciana C. C.
author_role author
author2 Cardoso, Fernanda C.
Miyasato, Patricia A.
Montoya, Bogar O.
Tararam, Cibele A.
Roffato, Henrique K.
Kawano, Toshie
Gazzinelli, Andréa Pereira
Oliveira, Rodrigo Corrêa de
Coulson, Patricia S.
Wilson, R. Alan
Oliveira, Sérgio Costa
Leite, Luciana C. C.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Farias, Leonardo P.
Cardoso, Fernanda C.
Miyasato, Patricia A.
Montoya, Bogar O.
Tararam, Cibele A.
Roffato, Henrique K.
Kawano, Toshie
Gazzinelli, Andréa Pereira
Oliveira, Rodrigo Corrêa de
Coulson, Patricia S.
Wilson, R. Alan
Oliveira, Sérgio Costa
Leite, Luciana C. C.
dc.subject.en.pt_BR.fl_str_mv Schistosomiasis
Schistosoma mansoni
topic Schistosomiasis
Schistosoma mansoni
description Instituto Butantan. Centro de Biotecnologia. Sao Paulo, SP, Brazil
publishDate 2010
dc.date.issued.fl_str_mv 2010
dc.date.accessioned.fl_str_mv 2012-09-27T16:30:55Z
dc.date.available.fl_str_mv 2012-09-27T16:30:55Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv FARIAS, Leonardo P et al. Schistosoma mansoni Stomatin Like Protein-2 Is Located in the Tegument and Induces Partial Protection against Challenge Infection. PLoS Negl Trop Dis. 2010 Feb 9;4(2):e597.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/5584
dc.identifier.issn.none.fl_str_mv 19352727
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pntd.0000597
identifier_str_mv FARIAS, Leonardo P et al. Schistosoma mansoni Stomatin Like Protein-2 Is Located in the Tegument and Induces Partial Protection against Challenge Infection. PLoS Negl Trop Dis. 2010 Feb 9;4(2):e597.
19352727
10.1371/journal.pntd.0000597
url https://www.arca.fiocruz.br/handle/icict/5584
dc.language.iso.fl_str_mv eng
language eng
dc.relation.isbasedon.pt_BR.fl_str_mv Farias LP, Cardoso FC, Miyasato PA, Montoya BO, Tararam CA, et al. (2010) Schistosoma mansoni Stomatin Like Protein-2 Is Located in the Tegument and Induces Partial Protection against Challenge Infection. PLoS Negl Trop Dis 4(2): e597
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
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