Antibody response to a new member of the DBL family (EBP2) after a brief Plasmodium vivax exposure
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2022 |
| Outros Autores: | , , , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/56622 |
Resumo: | Fundação Oswaldo Cruz. Instituto René Rachou. Biologia Molecular e Imunologia da Malária. Belo Horizonte, MG, Brazil. |
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Lima, Bárbara Andreza SoaresFernandes, Gabriela MariaTorres, Letícia de MenezesPires, Camilla ValenteAlves, Jéssica Rafaela dos SantosNascimento, Sâmick Layene MoreiraNascimento, Maria Fernanda Alves doAfonso, Sofia LaraCosta, Helena LofftCerávolo, Isabela PennaSousa, Tais NobregaSoares, Irene SNtumngia, Francis BabilaAdams, John HCarvalho, Luzia HelenaKano, Flora Satiko2023-01-25T19:24:51Z2023-01-25T19:24:51Z2022LIMA, Barbara Andreza Soares et al. Antibody response to a new member of the DBL family (EBP2) after a brief Plasmodium vivax exposure. PLoS Negl Trop Dis., v. 16, n. 6, e0010493, 2022. doi: 10.1371/journal.pntd.0010493.1935-2735https://www.arca.fiocruz.br/handle/icict/56622engPublic Library of ScienceAntibody response to a new member of the DBL family (EBP2) after a brief Plasmodium vivax exposureinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto René Rachou. Biologia Molecular e Imunologia da Malária. Belo Horizonte, MG, Brazil.Fundação Oswaldo Cruz. Instituto René Rachou. Biologia Molecular e Imunologia da Malária. Belo Horizonte, MG, Brazil.Fundação Oswaldo Cruz. Instituto René Rachou. Biologia Molecular e Imunologia da Malária. Belo Horizonte, MG, Brazil.Center for Global Health and Infectious Diseases Research. College of Public Health. University of South Florida. Tampa, Florida, United States of America.Fundação Oswaldo Cruz. Instituto René Rachou. Biologia Molecular e Imunologia da Malária. Belo Horizonte, MG, Brazil.Fundação Oswaldo Cruz. Instituto René Rachou. Biologia Molecular e Imunologia da Malária. Belo Horizonte, MG, Brazil.Fundação Oswaldo Cruz. Instituto René Rachou. Biologia Molecular e Imunologia da Malária. Belo Horizonte, MG, Brazil.Fundação Oswaldo Cruz. Instituto René Rachou. Biologia Molecular e Imunologia da Malária. Belo Horizonte, MG, Brazil.Fundação Oswaldo Cruz. Instituto René Rachou. Biologia Molecular e Imunologia da Malária. Belo Horizonte, MG, Brazil.Fundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Imunopatologia. Belo Horizonte, MG, Brazil.Fundação Oswaldo Cruz. Instituto René Rachou. Biologia Molecular e Imunologia da Malária. Belo Horizonte, MG, Brazil.Universidade de São Paulo. Faculdade de Ciências Farmacêuticas. Departamento de Análises Clínicas e Toxicológicas. São Paulo, SP, Brazil.Center for Global Health and Infectious Diseases Research. College of Public Health. University of South Florida. Tampa, Florida, United States of America.Center for Global Health and Infectious Diseases Research. College of Public Health. University of South Florida. Tampa, Florida, United States of America.Fundação Oswaldo Cruz. Instituto René Rachou. Biologia Molecular e Imunologia da Malária. Belo Horizonte, MG, Brazil.Fundação Oswaldo Cruz. Instituto René Rachou. Biologia Molecular e Imunologia da Malária. Belo Horizonte, MG, Brazil.Plasmodium vivax blood-stage invasion into reticulocyte is critical for parasite development. Thus, validation of novel parasite invasion ligands is essential for malaria vaccine development. Recently, we demonstrated that EBP2, a Duffy binding protein (DBP) paralog, is antigenically distinct from DBP and could not be functionally inhibited by anti-DBP antibodies. Here, we took advantage of a small outbreak of P.vivax malaria, located in a non-malarious area of Brazil, to investigate for the first time IgM/IgG antibodies against EBP2 and DEKnull-2 (an engineering DBPII vaccine) among individuals who had their first and brief exposure to P.vivax (16 cases and 22 non-cases). Our experimental approach included 4 cross sectional surveys at 3-month interval (12-month follow-up). The results demonstrated that while a brief initial P.vivax infection was not efficient to induce IgM/ IgG antibodies to either EBP2 or DEKnull-2, IgG antibodies against DEKnull-2 (but not EBP2) were boosted by recurrent blood-stage infections following treatment. Of interest, in most recurrent P. vivax infections (4 out of 6 patients) DEKnull-2 IgG antibodies were sustained for 6 to 12 months. Polymorphisms in the ebp2 gene does not seem to explain EBP2 low immunogenicity as the ebp2 allele associated with the P.vivax outbreak presented high identity to the original EBP2 isolate used as recombinant protein. Although EBP2 antibodies were barely detectable after a primary episode of P.vivax infection, EBP2 was highly recognized by serum IgG from long-term malaria-exposed Amazonians (range from 35 to 92% according to previous malaria episodes). Taken together, the results showed that individuals with a single and brief exposure to P.vivax infection develop very low anti-EBP2 antibodies, which tend to increase after long-term malaria exposure. Finally, the findings highlighted the potential of DEKnull-2 as a vaccine candidate, as in non-immune individuals anti-DEKnull-2 IgG antibodies were boosted even after a brief exposure to P.vivax blood stages.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/56622/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALAntibody response to a new member of the DBL.pdfAntibody response to a new member of the DBL.pdfapplication/pdf2122939https://www.arca.fiocruz.br/bitstream/icict/56622/2/Antibody%20response%20to%20a%20new%20member%20of%20the%20DBL.pdfe4a643dff68eeac4577ef89b2721c234MD52icict/566222023-01-25 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Antibody response to a new member of the DBL family (EBP2) after a brief Plasmodium vivax exposure |
| title |
Antibody response to a new member of the DBL family (EBP2) after a brief Plasmodium vivax exposure |
| spellingShingle |
Antibody response to a new member of the DBL family (EBP2) after a brief Plasmodium vivax exposure Lima, Bárbara Andreza Soares |
| title_short |
Antibody response to a new member of the DBL family (EBP2) after a brief Plasmodium vivax exposure |
| title_full |
Antibody response to a new member of the DBL family (EBP2) after a brief Plasmodium vivax exposure |
| title_fullStr |
Antibody response to a new member of the DBL family (EBP2) after a brief Plasmodium vivax exposure |
| title_full_unstemmed |
Antibody response to a new member of the DBL family (EBP2) after a brief Plasmodium vivax exposure |
| title_sort |
Antibody response to a new member of the DBL family (EBP2) after a brief Plasmodium vivax exposure |
| author |
Lima, Bárbara Andreza Soares |
| author_facet |
Lima, Bárbara Andreza Soares Fernandes, Gabriela Maria Torres, Letícia de Menezes Pires, Camilla Valente Alves, Jéssica Rafaela dos Santos Nascimento, Sâmick Layene Moreira Nascimento, Maria Fernanda Alves do Afonso, Sofia Lara Costa, Helena Lofft Cerávolo, Isabela Penna Sousa, Tais Nobrega Soares, Irene S Ntumngia, Francis Babila Adams, John H Carvalho, Luzia Helena Kano, Flora Satiko |
| author_role |
author |
| author2 |
Fernandes, Gabriela Maria Torres, Letícia de Menezes Pires, Camilla Valente Alves, Jéssica Rafaela dos Santos Nascimento, Sâmick Layene Moreira Nascimento, Maria Fernanda Alves do Afonso, Sofia Lara Costa, Helena Lofft Cerávolo, Isabela Penna Sousa, Tais Nobrega Soares, Irene S Ntumngia, Francis Babila Adams, John H Carvalho, Luzia Helena Kano, Flora Satiko |
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author author author author author author author author author author author author author author author |
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Lima, Bárbara Andreza Soares Fernandes, Gabriela Maria Torres, Letícia de Menezes Pires, Camilla Valente Alves, Jéssica Rafaela dos Santos Nascimento, Sâmick Layene Moreira Nascimento, Maria Fernanda Alves do Afonso, Sofia Lara Costa, Helena Lofft Cerávolo, Isabela Penna Sousa, Tais Nobrega Soares, Irene S Ntumngia, Francis Babila Adams, John H Carvalho, Luzia Helena Kano, Flora Satiko |
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Fundação Oswaldo Cruz. Instituto René Rachou. Biologia Molecular e Imunologia da Malária. Belo Horizonte, MG, Brazil. |
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2022 |
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LIMA, Barbara Andreza Soares et al. Antibody response to a new member of the DBL family (EBP2) after a brief Plasmodium vivax exposure. PLoS Negl Trop Dis., v. 16, n. 6, e0010493, 2022. doi: 10.1371/journal.pntd.0010493. |
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https://www.arca.fiocruz.br/handle/icict/56622 |
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1935-2735 |
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LIMA, Barbara Andreza Soares et al. Antibody response to a new member of the DBL family (EBP2) after a brief Plasmodium vivax exposure. PLoS Negl Trop Dis., v. 16, n. 6, e0010493, 2022. doi: 10.1371/journal.pntd.0010493. 1935-2735 |
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