Development of an enzyme-linked immunosorbent assay for the serodiagnosis of several clinical forms of sporotrichosis
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2005 |
| Outros Autores: | , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/33249 |
Resumo: | 2020-05-28 |
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Bernardes-Engemann, A. R.Costa, R. C.Miguens, B. R.Penha, C. V.Neves, E.Pereira, B. A.Dias, C. M.Mattos, M.Gutierrez, M. C.Schubach, A.Oliveira Neto, M. P.Lazéra, M. S.Bezerra, L. M. L.2019-05-28T13:47:47Z2019-05-28T13:47:47Z2005BERNARDES-ENGEMANN, A. R. et al. Development of an enzyme-linked immunosorbent assay for the serodiagnosis of several clinical forms of sporotrichosis. Medical Mycology, v. 43, p. 487-493, Sept. 2005.1369-3786https://www.arca.fiocruz.br/handle/icict/3324910.1080/136937804000199091460-2709engOxford University PressDevelopment of an enzyme-linked immunosorbent assay for the serodiagnosis of several clinical forms of sporotrichosisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2020-05-28Universidade do Estado do Rio de Janeiro. Laboratório de Biologia Celular e Bioquímica de Fungos Patogênicos. Rio de Janeiro, RJ, Brasil.Universidade do Estado do Rio de Janeiro. Hospital Universitário Pedro Ernesto. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.Universidade do Estado do Rio de Janeiro. Laboratório de Biologia Celular e Bioquímica de Fungos Patogênicos. Rio de Janeiro, RJ, Brasil.Universidade do Estado do Rio de Janeiro. Laboratório de Biologia Celular e Bioquímica de Fungos Patogênicos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Hospital Evandro Chagas. Rio de Janeiro, RJ, Brasil.Universidade do Estado do Rio de Janeiro. Laboratório de Biologia Celular e Bioquímica de Fungos Patogênicos. Rio de Janeiro, RJ, Brasil.Universidade do Estado do Rio de Janeiro. Hospital Universitário Pedro Ernesto. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Hospital Evandro Chagas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Hospital Evandro Chagas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Hospital Evandro Chagas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Hospital Evandro Chagas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Hospital Evandro Chagas. Rio de Janeiro, RJ, Brasil.Universidade do Estado do Rio de Janeiro. Laboratório de Biologia Celular e Bioquímica de Fungos Patogênicos. Rio de Janeiro, RJ, Brasil.We performed a serological study with sera from 92 patients with confirmed sporotrichosis registered between 1999 and 2004 in two hospitals in Rio de Janeiro State, Brazil. The clinical presentation of sporotrichosis was distributed as follows: lymphocutaneous, 67); fixed cutaneous, 23); disseminated cutaneous, 8); and extracutaneous, 2). Sera were assayed by ELISA against a cell wall antigen of Sporothrix schenckii, SsCBF, that we have previously described. The crossreactivity was determined with 77 heterologous sera. The serological test showed a sensitivity of 90) and a global efficiency of 86). A group of 55 patients with several clinical presentations of sporotrichosis was clinically and serologically followed-up for at least 6 months. We observed by ELISA data a decrease in the antibody serum titers which correlated with the progress in healing. An HIV positive patient with meningeal sporotrichosis was serologically followed-up for over 2 years. Serum and cerebrospinal fluid specimens were examined and significant antibodies levels against the antigen SsCBF were detected. Our results strongly suggest that this serological test is valuable for the differential diagnosis and follow-up of all clinical forms of sporotrichosis.AntigenCell wallS. schenckiiSerodiagnosisSporotrichosisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1748https://www.arca.fiocruz.br/bitstream/icict/33249/1/license.txt8a4605be74aa9ea9d79846c1fba20a33MD51ORIGINALve_Bernardes-Engemann_A_etal_INI_2005.pdfve_Bernardes-Engemann_A_etal_INI_2005.pdfapplication/pdf195487https://www.arca.fiocruz.br/bitstream/icict/33249/2/ve_Bernardes-Engemann_A_etal_INI_2005.pdf24416d98dd1ab9e2118661efa6828b62MD52TEXTve_Bernardes-Engemann_A_etal_INI_2005.pdf.txtve_Bernardes-Engemann_A_etal_INI_2005.pdf.txtExtracted texttext/plain30016https://www.arca.fiocruz.br/bitstream/icict/33249/3/ve_Bernardes-Engemann_A_etal_INI_2005.pdf.txt7e724e42d74ac7735f0923a5e0eb1c5bMD53icict/332492021-03-24 16:32:32.344oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352021-03-24T19:32:32Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.pt_BR.fl_str_mv |
Development of an enzyme-linked immunosorbent assay for the serodiagnosis of several clinical forms of sporotrichosis |
| title |
Development of an enzyme-linked immunosorbent assay for the serodiagnosis of several clinical forms of sporotrichosis |
| spellingShingle |
Development of an enzyme-linked immunosorbent assay for the serodiagnosis of several clinical forms of sporotrichosis Bernardes-Engemann, A. R. Antigen Cell wall S. schenckii Serodiagnosis Sporotrichosis |
| title_short |
Development of an enzyme-linked immunosorbent assay for the serodiagnosis of several clinical forms of sporotrichosis |
| title_full |
Development of an enzyme-linked immunosorbent assay for the serodiagnosis of several clinical forms of sporotrichosis |
| title_fullStr |
Development of an enzyme-linked immunosorbent assay for the serodiagnosis of several clinical forms of sporotrichosis |
| title_full_unstemmed |
Development of an enzyme-linked immunosorbent assay for the serodiagnosis of several clinical forms of sporotrichosis |
| title_sort |
Development of an enzyme-linked immunosorbent assay for the serodiagnosis of several clinical forms of sporotrichosis |
| author |
Bernardes-Engemann, A. R. |
| author_facet |
Bernardes-Engemann, A. R. Costa, R. C. Miguens, B. R. Penha, C. V. Neves, E. Pereira, B. A. Dias, C. M. Mattos, M. Gutierrez, M. C. Schubach, A. Oliveira Neto, M. P. Lazéra, M. S. Bezerra, L. M. L. |
| author_role |
author |
| author2 |
Costa, R. C. Miguens, B. R. Penha, C. V. Neves, E. Pereira, B. A. Dias, C. M. Mattos, M. Gutierrez, M. C. Schubach, A. Oliveira Neto, M. P. Lazéra, M. S. Bezerra, L. M. L. |
| author2_role |
author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Bernardes-Engemann, A. R. Costa, R. C. Miguens, B. R. Penha, C. V. Neves, E. Pereira, B. A. Dias, C. M. Mattos, M. Gutierrez, M. C. Schubach, A. Oliveira Neto, M. P. Lazéra, M. S. Bezerra, L. M. L. |
| dc.subject.en.pt_BR.fl_str_mv |
Antigen Cell wall S. schenckii Serodiagnosis Sporotrichosis |
| topic |
Antigen Cell wall S. schenckii Serodiagnosis Sporotrichosis |
| description |
2020-05-28 |
| publishDate |
2005 |
| dc.date.issued.fl_str_mv |
2005 |
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2019-05-28T13:47:47Z |
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2019-05-28T13:47:47Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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BERNARDES-ENGEMANN, A. R. et al. Development of an enzyme-linked immunosorbent assay for the serodiagnosis of several clinical forms of sporotrichosis. Medical Mycology, v. 43, p. 487-493, Sept. 2005. |
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https://www.arca.fiocruz.br/handle/icict/33249 |
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1369-3786 |
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10.1080/13693780400019909 |
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1460-2709 |
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BERNARDES-ENGEMANN, A. R. et al. Development of an enzyme-linked immunosorbent assay for the serodiagnosis of several clinical forms of sporotrichosis. Medical Mycology, v. 43, p. 487-493, Sept. 2005. 1369-3786 10.1080/13693780400019909 1460-2709 |
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Oxford University Press |
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Oxford University Press |
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