Agrobacterium - mediated transformation of Jatropha curcas leaf explants with a fungal chitinase gene

Detalhes bibliográficos
Autor(a) principal: Franco, Mariana Crotti
Data de Publicação: 2016
Outros Autores: Gomes, Kleber Alves, Carvalho Filho, Marcel Mamede de, Harakava, Ricardo, Carels, Nicolas, Siqueira, Walter José, Latado, Rodrigo Rocha, Marques, Daniela de Argollo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/23762
Resumo: Instituto Agronômico. Centro de P&D de Recursos Genéticos Vegetais. Campinas, SP, Brasil.
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spelling Franco, Mariana CrottiGomes, Kleber AlvesCarvalho Filho, Marcel Mamede deHarakava, RicardoCarels, NicolasSiqueira, Walter JoséLatado, Rodrigo RochaMarques, Daniela de Argollo2017-12-22T15:37:20Z2017-12-22T15:37:20Z2016Franco, M. C. Agrobacterium-mediated transformation of Jatropha curcas leaf explants with a fungal chitinase gene. African Journal of Biotechnology, v. 15, n. 37, p. 2006-2016, 2016.1684-5315https://www.arca.fiocruz.br/handle/icict/2376210.5897/AJB2016.15458engAcademic JournalsAgrobacteriumJatropha curcasAgrobacterium - mediated transformation of Jatropha curcas leaf explants with a fungal chitinase geneinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleInstituto Agronômico. Centro de P&D de Recursos Genéticos Vegetais. Campinas, SP, Brasil.Instituto Agronômico. Centro de P&D de Recursos Genéticos Vegetais. Campinas, SP, Brasil.Instituto Agronômico. Centro de P&D de Recursos Genéticos Vegetais. Campinas, SP, Brasil.Secretaria de Agricultura e Abastecimento. Instituto Biológico. Laboratório de Bioquímica Fitopatológica. São Paulo, SP. Brasil.Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Rio de Janeiro, RJ, Brasil.Instituto Agronômico. Centro de P&D de Recursos Genéticos Vegetais. Campinas, SP, Brasil.Instituto Agronômico. Centro APTA Citros Sylvio Moreira. Cordeirópolis, SP, Brasil.Instituto Agronômico. Centro de P&D de Recursos Genéticos Vegetais. Campinas, SP, Brasil.Jatropha curcas L. oil has been shown to be suitable for the production of biodiesel. However, this species has not been domesticated yet. Genetic breeding through conventional methods is time consuming and costly, hence, genetic transformation could contribute positively to the improvement of interesting traits. Although in vitro regeneration and stable genetic transformation has been pursued for several years, variation in transformation efficiency remains strongly genotype-dependent and indicates that protocols optimization is still needed. Thus, this study was carried out to introduce a chitinase gene from the Trichoderma viride fungus into the genome of a J. curcas superior genotype by inoculating leaf explants with Agrobacterium tumefaciens EHA 105 strain in axenic conditions. Some key parameters such as pre-culture period and antibiotic doses were optimized with 500 mg.L-1 cefotaxime and 100 mg.L-1 kanamycin concentrations being suitable for A. tumefaciens inhibition and explant selection, respectively. The best transformation efficiency (50%) was obtained when leaf explants were incubated on a culture medium promoting shoot regeneration at 15 days before the induction of the transformation process. Plants where chitinase gene amplicons could be detected were assessed for transgene copy number and expression levels by quantitative real-time polymerase chain reaction (PCR). One, two and three copies of the introduced gene were confirmed in nine transgenic lines with two of them that were assessed for gene expression and showed quantitative variation for this variable. These results bring valuable information for further gene insertions in breeding programs of J. curcas for fungal disease resistance.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83101https://www.arca.fiocruz.br/bitstream/icict/23762/1/license.txt425ef259bcb460998407fbfed7c102d0MD51ORIGINALCarels_etal_2016a.pdfCarels_etal_2016a.pdfapplication/pdf560819https://www.arca.fiocruz.br/bitstream/icict/23762/2/Carels_etal_2016a.pdf00e6e3495039812c44f90580bb1385bfMD52TEXTCarels_etal_2016a.pdf.txtCarels_etal_2016a.pdf.txtExtracted texttext/plain50719https://www.arca.fiocruz.br/bitstream/icict/23762/3/Carels_etal_2016a.pdf.txta91e1805317a5ba424b23040f5ab2607MD53icict/237622023-01-09 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dc.title.pt_BR.fl_str_mv Agrobacterium - mediated transformation of Jatropha curcas leaf explants with a fungal chitinase gene
title Agrobacterium - mediated transformation of Jatropha curcas leaf explants with a fungal chitinase gene
spellingShingle Agrobacterium - mediated transformation of Jatropha curcas leaf explants with a fungal chitinase gene
Franco, Mariana Crotti
Agrobacterium
Jatropha curcas
title_short Agrobacterium - mediated transformation of Jatropha curcas leaf explants with a fungal chitinase gene
title_full Agrobacterium - mediated transformation of Jatropha curcas leaf explants with a fungal chitinase gene
title_fullStr Agrobacterium - mediated transformation of Jatropha curcas leaf explants with a fungal chitinase gene
title_full_unstemmed Agrobacterium - mediated transformation of Jatropha curcas leaf explants with a fungal chitinase gene
title_sort Agrobacterium - mediated transformation of Jatropha curcas leaf explants with a fungal chitinase gene
author Franco, Mariana Crotti
author_facet Franco, Mariana Crotti
Gomes, Kleber Alves
Carvalho Filho, Marcel Mamede de
Harakava, Ricardo
Carels, Nicolas
Siqueira, Walter José
Latado, Rodrigo Rocha
Marques, Daniela de Argollo
author_role author
author2 Gomes, Kleber Alves
Carvalho Filho, Marcel Mamede de
Harakava, Ricardo
Carels, Nicolas
Siqueira, Walter José
Latado, Rodrigo Rocha
Marques, Daniela de Argollo
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Franco, Mariana Crotti
Gomes, Kleber Alves
Carvalho Filho, Marcel Mamede de
Harakava, Ricardo
Carels, Nicolas
Siqueira, Walter José
Latado, Rodrigo Rocha
Marques, Daniela de Argollo
dc.subject.other.pt_BR.fl_str_mv Agrobacterium
Jatropha curcas
topic Agrobacterium
Jatropha curcas
description Instituto Agronômico. Centro de P&D de Recursos Genéticos Vegetais. Campinas, SP, Brasil.
publishDate 2016
dc.date.issued.fl_str_mv 2016
dc.date.accessioned.fl_str_mv 2017-12-22T15:37:20Z
dc.date.available.fl_str_mv 2017-12-22T15:37:20Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv Franco, M. C. Agrobacterium-mediated transformation of Jatropha curcas leaf explants with a fungal chitinase gene. African Journal of Biotechnology, v. 15, n. 37, p. 2006-2016, 2016.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/23762
dc.identifier.issn.pt_BR.fl_str_mv 1684-5315
dc.identifier.doi.none.fl_str_mv 10.5897/AJB2016.15458
identifier_str_mv Franco, M. C. Agrobacterium-mediated transformation of Jatropha curcas leaf explants with a fungal chitinase gene. African Journal of Biotechnology, v. 15, n. 37, p. 2006-2016, 2016.
1684-5315
10.5897/AJB2016.15458
url https://www.arca.fiocruz.br/handle/icict/23762
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Academic Journals
publisher.none.fl_str_mv Academic Journals
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/23762/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/23762/2/Carels_etal_2016a.pdf
https://www.arca.fiocruz.br/bitstream/icict/23762/3/Carels_etal_2016a.pdf.txt
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