Growth conditions influence melanization of Brazilian clinical Sporothrix schenckii isolates

Detalhes bibliográficos
Autor(a) principal: Almeida-Paes, Rodrigo
Data de Publicação: 2009
Outros Autores: Frases, Susana, Monteiro, Paulo Cezar Fialho, Gutierrez-Galhardo, Maria Clara, Zancopé-Oliveira, Rosely Maria, Nosanchuk, Joshua D.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/33495
Resumo: 2020-06-13
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spelling Almeida-Paes, RodrigoFrases, SusanaMonteiro, Paulo Cezar FialhoGutierrez-Galhardo, Maria ClaraZancopé-Oliveira, Rosely MariaNosanchuk, Joshua D.2019-06-13T14:29:33Z2019-06-13T14:29:33Z2009ALMEIDA-PAES, Rodrigo et al. Growth conditions influence melanization of Brazilian clinical Sporothrix schenckii isolates. Microbes and Infection, n. 11, v. 5, p. 554-562, Apr. 2009.1286-4579https://www.arca.fiocruz.br/handle/icict/3349510.1016/j.micinf.2009.03.0021769-714XengElsevierGrowth conditions influence melanization of Brazilian clinical Sporothrix schenckii isolatesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2020-06-13Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratórios de Micologia. Rio de Janeiro, RJ, Brasil / Albert Einstein College of Medicine. Department of Medicine. Division of Infectious Diseases. New York, NY, USA.Albert Einstein College of Medicine. Department of Microbiology and Immunology. New York, NY, USA.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratórios de Micologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Dermatologia Infecciosa. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratórios de Micologia. Rio de Janeiro, RJ, Brasil.Albert Einstein College of Medicine. Department of Medicine. Division of Infectious Diseases. New York, NY, USA / Albert Einstein College of Medicine. Department of Microbiology and Immunology. New York, NY, USA.Sporothrix schenckii is known to produce DHN melanin on both conidial and yeast cells, however little information is available regarding the factors inducing fungal melanization. We evaluated whether culture conditions influenced melanization of 25 Brazilian S. schenckii strains and one control strain (ATCC 10212). Tested conditions included different media, pH, temperature, incubation time, glucose concentrations, and presence or absence of tricyclazole or L-DOPA. Melanization was reduced on Sabouraud compared to defined chemical medium. The majority of strains produced small amounts of melanin at 37°C and none melanized at basic pH. Increased glucose concentrations did not inhibit melanization, rather increasing glucose enhanced pigment production in 27% of strains. Melanin synthesis was also enhanced by the addition of L-DOPA and its addition to medium with tricyclazole, an inhibitor of melanin synthesis, resulted in fungal melanization, including hyphal melanin production. Our results suggest that different S. schenckii strains have distinct control of melanization and that this fungus can use phenolic compounds to enhance melanization in vitro.Sporothrix schenckiiMelaninCulture conditionsinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1748https://www.arca.fiocruz.br/bitstream/icict/33495/1/license.txt8a4605be74aa9ea9d79846c1fba20a33MD51ORIGINALve_Almeida-Paes_Rodrigo_etal_INI_2009.pdfve_Almeida-Paes_Rodrigo_etal_INI_2009.pdfapplication/pdf918803https://www.arca.fiocruz.br/bitstream/icict/33495/2/ve_Almeida-Paes_Rodrigo_etal_INI_2009.pdf85ef54d4db64c82e88b4f3415b6759c8MD52TEXTve_Almeida-Paes_Rodrigo_etal_INI_2009.pdf.txtve_Almeida-Paes_Rodrigo_etal_INI_2009.pdf.txtExtracted texttext/plain39670https://www.arca.fiocruz.br/bitstream/icict/33495/3/ve_Almeida-Paes_Rodrigo_etal_INI_2009.pdf.txt11f30c844486c6cf23dc314e18e82b91MD53icict/334952021-02-10 19:38:15.145oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352021-02-10T22:38:15Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Growth conditions influence melanization of Brazilian clinical Sporothrix schenckii isolates
title Growth conditions influence melanization of Brazilian clinical Sporothrix schenckii isolates
spellingShingle Growth conditions influence melanization of Brazilian clinical Sporothrix schenckii isolates
Almeida-Paes, Rodrigo
Sporothrix schenckii
Melanin
Culture conditions
title_short Growth conditions influence melanization of Brazilian clinical Sporothrix schenckii isolates
title_full Growth conditions influence melanization of Brazilian clinical Sporothrix schenckii isolates
title_fullStr Growth conditions influence melanization of Brazilian clinical Sporothrix schenckii isolates
title_full_unstemmed Growth conditions influence melanization of Brazilian clinical Sporothrix schenckii isolates
title_sort Growth conditions influence melanization of Brazilian clinical Sporothrix schenckii isolates
author Almeida-Paes, Rodrigo
author_facet Almeida-Paes, Rodrigo
Frases, Susana
Monteiro, Paulo Cezar Fialho
Gutierrez-Galhardo, Maria Clara
Zancopé-Oliveira, Rosely Maria
Nosanchuk, Joshua D.
author_role author
author2 Frases, Susana
Monteiro, Paulo Cezar Fialho
Gutierrez-Galhardo, Maria Clara
Zancopé-Oliveira, Rosely Maria
Nosanchuk, Joshua D.
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Almeida-Paes, Rodrigo
Frases, Susana
Monteiro, Paulo Cezar Fialho
Gutierrez-Galhardo, Maria Clara
Zancopé-Oliveira, Rosely Maria
Nosanchuk, Joshua D.
dc.subject.en.pt_BR.fl_str_mv Sporothrix schenckii
Melanin
Culture conditions
topic Sporothrix schenckii
Melanin
Culture conditions
description 2020-06-13
publishDate 2009
dc.date.issued.fl_str_mv 2009
dc.date.accessioned.fl_str_mv 2019-06-13T14:29:33Z
dc.date.available.fl_str_mv 2019-06-13T14:29:33Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv ALMEIDA-PAES, Rodrigo et al. Growth conditions influence melanization of Brazilian clinical Sporothrix schenckii isolates. Microbes and Infection, n. 11, v. 5, p. 554-562, Apr. 2009.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/33495
dc.identifier.issn.pt_BR.fl_str_mv 1286-4579
dc.identifier.doi.none.fl_str_mv 10.1016/j.micinf.2009.03.002
dc.identifier.eissn.none.fl_str_mv 1769-714X
identifier_str_mv ALMEIDA-PAES, Rodrigo et al. Growth conditions influence melanization of Brazilian clinical Sporothrix schenckii isolates. Microbes and Infection, n. 11, v. 5, p. 554-562, Apr. 2009.
1286-4579
10.1016/j.micinf.2009.03.002
1769-714X
url https://www.arca.fiocruz.br/handle/icict/33495
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
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