Intranasal Flunisolide Suppresses Pathological Alterations Caused by Silica Particles in the Lungs of Mice
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2020 |
| Outros Autores: | , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/45484 |
Resumo: | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil. |
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Ferreira, Tatiana Paula TeixeiraLima, Januário Gomes Mourão eFarias Filho, Francisco AlvesSá, Yago Amigo Pinho Jannini deArantes, Ana Carolina Santos deGuimarães, Fernanda VerdiniCarvalho, Vinicius de FriasHogaboam, CoryWallace, JohnMartins, Marco AurélioSilva, Patrícia Machado Rodrigues e2021-01-01T19:21:36Z2021-01-01T19:21:36Z2020FERREIRA, Tatiana Paula Teixeira et al. Intranasal Flunisolide Suppresses Pathological Alterations Caused by Silica Particles in the Lungs of Mice. Fronties in Endocrinology, v. 11, Article 388, p. 1-12, June 2000.1664-2392https://www.arca.fiocruz.br/handle/icict/4548410.3389/fendo.2020.00388engFrontiers MediaPulmãoFibrosePartículas de sílicaTerapiaFlunisolidaLungFibrosisSilica particlesTherapyFlunisolideIntranasal Flunisolide Suppresses Pathological Alterations Caused by Silica Particles in the Lungs of Miceinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil.Women's Guild Lung Institute. Cedars-Sinai Medical Center. Department of Medicine. Los Angeles, CA, USA.University of Calgary. Cumming School of Medicine. Departments of Physiology and Pharmacology, and Medicine. Calgary, AB, Canada.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil.Silicosis is an occupational disease triggered by the inhalation of fine particles of crystalline silica and characterized by inflammation and scarring in the form of nodular lesions in the lungs. In spite of the therapeutic arsenal currently available, there is no specific treatment for the disease. Flunisolide is a potent corticosteroid shown to be effective for controlling chronic lung inflammatory diseases. In this study, the effect of flunisolide on silica-induced lung pathological changes in mice was investigated. Swiss-Webster mice were injected intranasally with silica particles and further treated with flunisolide from day 21 to 27 post-silica challenge. Lung function was assessed by whole body invasive plethysmography. Granuloma formation was evaluated morphometrically, collagen deposition by Picrus sirius staining and quantitated by Sircol. Chemokines and cytokines were evaluated using enzyme-linked immunosorbent assay. The sensitivity of lung fibroblasts was also examined in in vitro assays. Silica challenge led to increased leukocyte numbers (mononuclear cells and neutrophils) as well as production of the chemokine KC/CXCL-1 and the cytokines TNF-α and TGF-β in the bronchoalveolar lavage. These alterations paralleled to progressive granuloma formation, collagen deposition and impairment of lung function. Therapeutic administration of intranasal flunisolide inhibited granuloma and fibrotic responses, noted 28 days after silica challenge. The upregulation of MIP-1α/CCL-3 and MIP-2/CXCL-2 and the cytokines TNF-α and TGF-β, as well as deposition of collagen and airway hyper-reactivity to methacholine were shown to be clearly sensitive to flunisolide, as compared to silica-challenge untreated mice. Additionally, flunisolide effectively suppressed the responses of proliferation and MCP-1/CCL-2 production from IL-13 stimulated lung fibroblasts from silica- or saline-challenged mice. In conclusion, we report that intranasal treatment with the corticosteroid flunisolide showed protective properties on pathological features triggered by silica particles in mice, suggesting that the compound may constitute a promising strategy for the treatment of silicosis.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/45484/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALTatianaPT_Ferreira_etal_IOC_2020.pdfTatianaPT_Ferreira_etal_IOC_2020.pdfapplication/pdf3029450https://www.arca.fiocruz.br/bitstream/icict/45484/2/TatianaPT_Ferreira_etal_IOC_2020.pdf7c75c0088ce177efad2f319ad5ef8a71MD52TEXTTatianaPT_Ferreira_etal_IOC_2020.pdf.txtTatianaPT_Ferreira_etal_IOC_2020.pdf.txtExtracted 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| dc.title.pt_BR.fl_str_mv |
Intranasal Flunisolide Suppresses Pathological Alterations Caused by Silica Particles in the Lungs of Mice |
| title |
Intranasal Flunisolide Suppresses Pathological Alterations Caused by Silica Particles in the Lungs of Mice |
| spellingShingle |
Intranasal Flunisolide Suppresses Pathological Alterations Caused by Silica Particles in the Lungs of Mice Ferreira, Tatiana Paula Teixeira Pulmão Fibrose Partículas de sílica Terapia Flunisolida Lung Fibrosis Silica particles Therapy Flunisolide |
| title_short |
Intranasal Flunisolide Suppresses Pathological Alterations Caused by Silica Particles in the Lungs of Mice |
| title_full |
Intranasal Flunisolide Suppresses Pathological Alterations Caused by Silica Particles in the Lungs of Mice |
| title_fullStr |
Intranasal Flunisolide Suppresses Pathological Alterations Caused by Silica Particles in the Lungs of Mice |
| title_full_unstemmed |
Intranasal Flunisolide Suppresses Pathological Alterations Caused by Silica Particles in the Lungs of Mice |
| title_sort |
Intranasal Flunisolide Suppresses Pathological Alterations Caused by Silica Particles in the Lungs of Mice |
| author |
Ferreira, Tatiana Paula Teixeira |
| author_facet |
Ferreira, Tatiana Paula Teixeira Lima, Januário Gomes Mourão e Farias Filho, Francisco Alves Sá, Yago Amigo Pinho Jannini de Arantes, Ana Carolina Santos de Guimarães, Fernanda Verdini Carvalho, Vinicius de Frias Hogaboam, Cory Wallace, John Martins, Marco Aurélio Silva, Patrícia Machado Rodrigues e |
| author_role |
author |
| author2 |
Lima, Januário Gomes Mourão e Farias Filho, Francisco Alves Sá, Yago Amigo Pinho Jannini de Arantes, Ana Carolina Santos de Guimarães, Fernanda Verdini Carvalho, Vinicius de Frias Hogaboam, Cory Wallace, John Martins, Marco Aurélio Silva, Patrícia Machado Rodrigues e |
| author2_role |
author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Ferreira, Tatiana Paula Teixeira Lima, Januário Gomes Mourão e Farias Filho, Francisco Alves Sá, Yago Amigo Pinho Jannini de Arantes, Ana Carolina Santos de Guimarães, Fernanda Verdini Carvalho, Vinicius de Frias Hogaboam, Cory Wallace, John Martins, Marco Aurélio Silva, Patrícia Machado Rodrigues e |
| dc.subject.other.pt_BR.fl_str_mv |
Pulmão Fibrose Partículas de sílica Terapia Flunisolida |
| topic |
Pulmão Fibrose Partículas de sílica Terapia Flunisolida Lung Fibrosis Silica particles Therapy Flunisolide |
| dc.subject.en.pt_BR.fl_str_mv |
Lung Fibrosis Silica particles Therapy Flunisolide |
| description |
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil. |
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2020 |
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2020 |
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2021-01-01T19:21:36Z |
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2021-01-01T19:21:36Z |
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info:eu-repo/semantics/article |
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article |
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FERREIRA, Tatiana Paula Teixeira et al. Intranasal Flunisolide Suppresses Pathological Alterations Caused by Silica Particles in the Lungs of Mice. Fronties in Endocrinology, v. 11, Article 388, p. 1-12, June 2000. |
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https://www.arca.fiocruz.br/handle/icict/45484 |
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1664-2392 |
| dc.identifier.doi.none.fl_str_mv |
10.3389/fendo.2020.00388 |
| identifier_str_mv |
FERREIRA, Tatiana Paula Teixeira et al. Intranasal Flunisolide Suppresses Pathological Alterations Caused by Silica Particles in the Lungs of Mice. Fronties in Endocrinology, v. 11, Article 388, p. 1-12, June 2000. 1664-2392 10.3389/fendo.2020.00388 |
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Frontiers Media |
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