Analytical Validation of Quantitative Real-Time PCR Methods for Quantification of Trypanosoma cruzi DNA in Blood Samples from Chagas Disease Patients

Ramirez, Juan Carlos; Cura, Carolina Inés; Moreira, Otacilio da Cruz; Silva, Eliane Lages; Juiz, Natalia; Velásquez, Elsa; Ramirez, Juan David; Alberti, Anahi; Pavia, Paula; Flores-Chávez, Maria Delmans; Muñoz-Calderón, Arturo; Pérez-Morales, Deyanira; Santalia, José; Guedes, Paulo Marcos da Matta; Peneau, Julie; Marcet, Paula; Padilla, Carlos; Cruz-Robles, David; Valencia, Edward; Crisante, Gladys Elena; Greif, Gonzalo; Zulantay, Inés; Costales, Jaime Alfredo; Alvarez-Martínez, Miriam; Martinez, Norma Edith; Villarroel, Rodrigo; Villarroel, Sandro; Sánchez, Zunilda; Bisio, Margarita; Parrado, Rudy; Galvão, Lúcia Maria da Cunha; Câmara, Antonia Cláudia Jácome da; Espinoza, Bertha; Noya, Belkisyole Alarcón de; Puerta, Concepción; Riarte, Adelina; Diosque, Patricio; Sosa-Estani, Sergio; Guhl, Felipe; Ribeiro, Isabela; Aznar, Christine; Brito, Constança; Yadón, Zaída Estela; Schijman, Alejandro G.

Analytical Validation of Quantitative Real-Time PCR Methods for Quantification of Trypanosoma cruzi DNA in Blood Samples from Chagas Disease Patients

Detalhes bibliográficos
Autor(a) principal:	Ramirez, Juan Carlos
Data de Publicação:	2015
Outros Autores:	Cura, Carolina Inés, Moreira, Otacilio da Cruz, Silva, Eliane Lages, Juiz, Natalia, Velásquez, Elsa, Ramirez, Juan David, Alberti, Anahi, Pavia, Paula, Flores-Chávez, Maria Delmans, Muñoz-Calderón, Arturo, Pérez-Morales, Deyanira, Santalia, José, Guedes, Paulo Marcos da Matta, Peneau, Julie, Marcet, Paula, Padilla, Carlos, Cruz-Robles, David, Valencia, Edward, Crisante, Gladys Elena, Greif, Gonzalo, Zulantay, Inés, Costales, Jaime Alfredo, Alvarez-Martínez, Miriam, Martinez, Norma Edith, Villarroel, Rodrigo, Villarroel, Sandro, Sánchez, Zunilda, Bisio, Margarita, Parrado, Rudy, Galvão, Lúcia Maria da Cunha, Câmara, Antonia Cláudia Jácome da, Espinoza, Bertha, Noya, Belkisyole Alarcón de, Puerta, Concepción, Riarte, Adelina, Diosque, Patricio, Sosa-Estani, Sergio, Guhl, Felipe, Ribeiro, Isabela, Aznar, Christine, Brito, Constança, Yadón, Zaída Estela, Schijman, Alejandro G.
Tipo de documento:	Artigo
Idioma:	eng
Título da fonte:	Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo:	https://www.arca.fiocruz.br/handle/icict/12920
Resumo:	Instituto de Investigaciones en Ingeniería Genética y Biología Molecular (INGEBI-CONICET). Laboratory of Molecular Biology of Chagas Disease (LaBMECh). Buenos Aires, Argentina.

Metadados do item

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oai_identifier_str	oai:www.arca.fiocruz.br:icict/12920
network_acronym_str	CRUZ
network_name_str	Repositório Institucional da FIOCRUZ (ARCA)
repository_id_str	2135
spelling	Ramirez, Juan CarlosCura, Carolina InésMoreira, Otacilio da CruzSilva, Eliane LagesJuiz, NataliaVelásquez, ElsaRamirez, Juan DavidAlberti, AnahiPavia, PaulaFlores-Chávez, Maria DelmansMuñoz-Calderón, ArturoPérez-Morales, DeyaniraSantalia, JoséGuedes, Paulo Marcos da MattaPeneau, JulieMarcet, PaulaPadilla, CarlosCruz-Robles, DavidValencia, EdwardCrisante, Gladys ElenaGreif, GonzaloZulantay, InésCostales, Jaime AlfredoAlvarez-Martínez, MiriamMartinez, Norma EdithVillarroel, RodrigoVillarroel, SandroSánchez, ZunildaBisio, MargaritaParrado, RudyGalvão, Lúcia Maria da CunhaCâmara, Antonia Cláudia Jácome daEspinoza, BerthaNoya, Belkisyole Alarcón dePuerta, ConcepciónRiarte, AdelinaDiosque, PatricioSosa-Estani, SergioGuhl, FelipeRibeiro, IsabelaAznar, ChristineBrito, ConstançaYadón, Zaída EstelaSchijman, Alejandro G.2016-03-01T14:37:34Z2016-03-01T14:37:34Z2015RAMIREZ, Juan Carlos; et al. Analytical Validation of Quantitative Real-Time PCR Methods for Quantification of Trypanosoma cruzi DNA in Blood Samples from Chagas Disease Patients. J Mol Diagn., v.17, n.5, p.605-615, Sept. 2015.1525-1578https://www.arca.fiocruz.br/handle/icict/1292010.1016/j.jmoldx.2015.04.0101943-7811engElsevierAmostras de SangueChagas diseaseTrypanosoma cruziQuantitative Real-Time PCR MethodsBlood SamplesReação em Cadeia da PolimeraseDoença de ChagasTrypanosoma cruziAnalytical Validation of Quantitative Real-Time PCR Methods for Quantification of Trypanosoma cruzi DNA in Blood Samples from Chagas Disease Patientsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleInstituto de Investigaciones en Ingeniería Genética y Biología Molecular (INGEBI-CONICET). Laboratory of Molecular Biology of Chagas Disease (LaBMECh). Buenos Aires, Argentina.Instituto de Investigaciones en Ingeniería Genética y Biología Molecular (INGEBI-CONICET). Laboratory of Molecular Biology of Chagas Disease (LaBMECh). Buenos Aires, Argentina.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil.Universidade Federal do Triângulo Mineiro. Laboratório da Disciplina de Patologia. Uberaba, MG, Brasil.Instituto de Investigaciones en Ingeniería Genética y Biología Molecular (INGEBI-CONICET). Laboratory of Molecular Biology of Chagas Disease (LaBMECh). Buenos Aires, Argentina.National Institute of Parasitology “Dr. Mario Fatala Chabén”. Buenos Aires, Argentina.Universidad de Los Andes. Center for Research in Tropical Microbiology and Parasitology. Bogotá, Colombia.CONICET-Universidad Nacional de Salta. The Institute of Experimental Pathology. Salta, Argentina.Pontificia Universidad Javeriana. Laboratory of Molecular Parasitology. Bogota, Colombia.Instituto de Salud Carlos III. National Center for Microbiology. Madrid, Spain.Universidad Central de Venezuela. Institute of Tropical Medicine. Caracas, Venezuela.Universidad Nacional Autónoma de México. Biomedical Research Institute. Mexico, DF, Mexico.Instituto Nacional de Laboratorios en Salud. Laboratory of Parasitology and Molecular Biology. La Paz, Bolivia.Universidade Federal do Rio Grande do Norte. Departamento de Microbiologia e Parasitologia. Natal, RN, Brasil.Hospital and University LaboratoryCH Andrée Rosemon. Cayenne, French Guiana.Centers for Disease Control and Prevention. Division of Parasitic Diseases and Malaria. Atlanta, Georgia, USA.Instituto Nacional de Salud. National Center for Public Health. Lima, Peru.Instituto Nacional de Cardiología “Ignacio Chávez”. Laboratory of Genomics. Mexico, DF, Mexico.Universidad Peruana Cayetano Heredia. Laboratory for Research in Infectious Diseases. Lima, Peru.Universidad de los Andes. Department of Biology. Merida, Venezuela.Instituto Pasteur de Montevideo. Molecular Biology Unit. Montevideo, Uruguay.Universidad de Chile. Basic Clinical Parasitology Laboratory. Santiago, Chile.Pontificia Universidad Católica de Ecuador. Research Center for Infectious Diseases. Quito, Ecuador.Hospital Clinic and Barcelona Centre for International Health Research (CRESIB). Microbiology Department. Barcelona, Spain.State Laboratory of Public Health. Acapulco, Mexico.Instituto de Salud Pública. Biomedical Department. Santiago, Chile.Universidad Mayor de San Simón. Laboratory of Molecular Biology. Cochabamba, Bolivia.Universidad Nacional de Asunción. Research Institute for Health Sciences. Asuncion, Paraguay.Instituto de Investigaciones en Ingeniería Genética y Biología Molecular (INGEBI-CONICET). Laboratory of Molecular Biology of Chagas Disease (LaBMECh). Buenos Aires, Argentina.Universidad Mayor de San Simón. Laboratory of Molecular Biology. Cochabamba, Bolivia.Universidade Federal do Rio Grande do Norte. Departamento de Microbiologia e Parasitologia. Natal, RN, Brasil.Universidade Federal do Rio Grande do Norte. Departamento de Microbiologia e Parasitologia. Natal, RN, Brasil.Universidad Nacional Autónoma de México. Biomedical Research Institute. Mexico, DF, Mexico.Universidad Central de Venezuela. Institute of Tropical Medicine. Caracas, Venezuela.Pontificia Universidad Javeriana. Laboratory of Molecular Parasitology. Bogota, Colombia.National Institute of Parasitology “Dr. Mario Fatala Chabén”. Buenos Aires, Argentina.CONICET-Universidad Nacional de Salta. The Institute of Experimental Pathology. Salta, Argentina.National Institute of Parasitology “Dr. Mario Fatala Chabén”. Buenos Aires, Argentina.Universidad de Los Andes. Center for Research in Tropical Microbiology and Parasitology. Bogotá, Colombia.Drugs for Neglected Diseases Initiative (DNDi). Geneva, SwitzerlandHospital and University LaboratoryCH Andrée Rosemon. Cayenne, French Guiana.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil.Pan American Health Organization/World Health Organization (PAHO). Communicable Diseases and Health Analysis Department/WHO). Rio de Janeiro, RJ, Brasil.Instituto de Investigaciones en Ingeniería Genética y Biología Molecular (INGEBI-CONICET). Laboratory of Molecular Biology of Chagas Disease (LaBMECh). Buenos Aires, Argentina.An international study was performed by 26 experienced PCR laboratories from 14 countries to assess the performance of duplex quantitative real-time PCR (qPCR) strategies on the basis of TaqMan probes for detection and quantification of parasitic loads in peripheral blood samples from Chagas disease patients. Two methods were studied: Satellite DNA (SatDNA) qPCR and kinetoplastid DNA (kDNA) qPCR. Both methods included an internal amplification control. Reportable range, analytical sensitivity, limits of detection and quantification, and precision were estimated according to international guidelines. In addition, inclusivity and exclusivity were estimated with DNA from stocks representing the different Trypanosoma cruzi discrete typing units and Trypanosoma rangeli and Leishmania spp. Both methods were challenged against 156 blood samples provided by the participant laboratories, including samples from acute and chronic patients with varied clinical findings, infected by oral route or vectorial transmission. kDNA qPCR showed better analytical sensitivity than SatDNA qPCR with limits of detection of 0.23 and 0.70 parasite equivalents/mL, respectively. Analyses of clinical samples revealed a high concordance in terms of sensitivity and parasitic loads determined by both SatDNA and kDNA qPCRs. This effort is a major step toward international validation of qPCR methods for the quantification of T. cruzi DNA in human blood samples, aiming to provide an accurate surrogate biomarker for diagnosis and treatment monitoring for patients with Chagas disease.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/12920/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALconstança_brito_etal_IOC_2015.pdfconstança_brito_etal_IOC_2015.pdfapplication/pdf498816https://www.arca.fiocruz.br/bitstream/icict/12920/2/constan%c3%a7a_brito_etal_IOC_2015.pdfa4657816d73aedff572e1e40cccfba4aMD52TEXTconstança_brito_etal_IOC_2015.pdf.txtconstança_brito_etal_IOC_2015.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv	Analytical Validation of Quantitative Real-Time PCR Methods for Quantification of Trypanosoma cruzi DNA in Blood Samples from Chagas Disease Patients
title	Analytical Validation of Quantitative Real-Time PCR Methods for Quantification of Trypanosoma cruzi DNA in Blood Samples from Chagas Disease Patients
spellingShingle	Analytical Validation of Quantitative Real-Time PCR Methods for Quantification of Trypanosoma cruzi DNA in Blood Samples from Chagas Disease Patients Ramirez, Juan Carlos Amostras de Sangue Chagas disease Trypanosoma cruzi Quantitative Real-Time PCR Methods Blood Samples Reação em Cadeia da Polimerase Doença de Chagas Trypanosoma cruzi
title_short	Analytical Validation of Quantitative Real-Time PCR Methods for Quantification of Trypanosoma cruzi DNA in Blood Samples from Chagas Disease Patients
title_full	Analytical Validation of Quantitative Real-Time PCR Methods for Quantification of Trypanosoma cruzi DNA in Blood Samples from Chagas Disease Patients
title_fullStr	Analytical Validation of Quantitative Real-Time PCR Methods for Quantification of Trypanosoma cruzi DNA in Blood Samples from Chagas Disease Patients
title_full_unstemmed	Analytical Validation of Quantitative Real-Time PCR Methods for Quantification of Trypanosoma cruzi DNA in Blood Samples from Chagas Disease Patients
title_sort	Analytical Validation of Quantitative Real-Time PCR Methods for Quantification of Trypanosoma cruzi DNA in Blood Samples from Chagas Disease Patients
author	Ramirez, Juan Carlos
author_facet	Ramirez, Juan Carlos Cura, Carolina Inés Moreira, Otacilio da Cruz Silva, Eliane Lages Juiz, Natalia Velásquez, Elsa Ramirez, Juan David Alberti, Anahi Pavia, Paula Flores-Chávez, Maria Delmans Muñoz-Calderón, Arturo Pérez-Morales, Deyanira Santalia, José Guedes, Paulo Marcos da Matta Peneau, Julie Marcet, Paula Padilla, Carlos Cruz-Robles, David Valencia, Edward Crisante, Gladys Elena Greif, Gonzalo Zulantay, Inés Costales, Jaime Alfredo Alvarez-Martínez, Miriam Martinez, Norma Edith Villarroel, Rodrigo Villarroel, Sandro Sánchez, Zunilda Bisio, Margarita Parrado, Rudy Galvão, Lúcia Maria da Cunha Câmara, Antonia Cláudia Jácome da Espinoza, Bertha Noya, Belkisyole Alarcón de Puerta, Concepción Riarte, Adelina Diosque, Patricio Sosa-Estani, Sergio Guhl, Felipe Ribeiro, Isabela Aznar, Christine Brito, Constança Yadón, Zaída Estela Schijman, Alejandro G.
author_role	author
author2	Cura, Carolina Inés Moreira, Otacilio da Cruz Silva, Eliane Lages Juiz, Natalia Velásquez, Elsa Ramirez, Juan David Alberti, Anahi Pavia, Paula Flores-Chávez, Maria Delmans Muñoz-Calderón, Arturo Pérez-Morales, Deyanira Santalia, José Guedes, Paulo Marcos da Matta Peneau, Julie Marcet, Paula Padilla, Carlos Cruz-Robles, David Valencia, Edward Crisante, Gladys Elena Greif, Gonzalo Zulantay, Inés Costales, Jaime Alfredo Alvarez-Martínez, Miriam Martinez, Norma Edith Villarroel, Rodrigo Villarroel, Sandro Sánchez, Zunilda Bisio, Margarita Parrado, Rudy Galvão, Lúcia Maria da Cunha Câmara, Antonia Cláudia Jácome da Espinoza, Bertha Noya, Belkisyole Alarcón de Puerta, Concepción Riarte, Adelina Diosque, Patricio Sosa-Estani, Sergio Guhl, Felipe Ribeiro, Isabela Aznar, Christine Brito, Constança Yadón, Zaída Estela Schijman, Alejandro G.
author2_role	author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author
dc.contributor.author.fl_str_mv	Ramirez, Juan Carlos Cura, Carolina Inés Moreira, Otacilio da Cruz Silva, Eliane Lages Juiz, Natalia Velásquez, Elsa Ramirez, Juan David Alberti, Anahi Pavia, Paula Flores-Chávez, Maria Delmans Muñoz-Calderón, Arturo Pérez-Morales, Deyanira Santalia, José Guedes, Paulo Marcos da Matta Peneau, Julie Marcet, Paula Padilla, Carlos Cruz-Robles, David Valencia, Edward Crisante, Gladys Elena Greif, Gonzalo Zulantay, Inés Costales, Jaime Alfredo Alvarez-Martínez, Miriam Martinez, Norma Edith Villarroel, Rodrigo Villarroel, Sandro Sánchez, Zunilda Bisio, Margarita Parrado, Rudy Galvão, Lúcia Maria da Cunha Câmara, Antonia Cláudia Jácome da Espinoza, Bertha Noya, Belkisyole Alarcón de Puerta, Concepción Riarte, Adelina Diosque, Patricio Sosa-Estani, Sergio Guhl, Felipe Ribeiro, Isabela Aznar, Christine Brito, Constança Yadón, Zaída Estela Schijman, Alejandro G.
dc.subject.other.pt_BR.fl_str_mv	Amostras de Sangue
topic	Amostras de Sangue Chagas disease Trypanosoma cruzi Quantitative Real-Time PCR Methods Blood Samples Reação em Cadeia da Polimerase Doença de Chagas Trypanosoma cruzi
dc.subject.en.pt_BR.fl_str_mv	Chagas disease Trypanosoma cruzi Quantitative Real-Time PCR Methods Blood Samples
dc.subject.decs.pt_BR.fl_str_mv	Reação em Cadeia da Polimerase Doença de Chagas Trypanosoma cruzi
description	Instituto de Investigaciones en Ingeniería Genética y Biología Molecular (INGEBI-CONICET). Laboratory of Molecular Biology of Chagas Disease (LaBMECh). Buenos Aires, Argentina.
publishDate	2015
dc.date.issued.fl_str_mv	2015
dc.date.accessioned.fl_str_mv	2016-03-01T14:37:34Z
dc.date.available.fl_str_mv	2016-03-01T14:37:34Z
dc.type.status.fl_str_mv	info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv	info:eu-repo/semantics/article
format	article
status_str	publishedVersion
dc.identifier.citation.fl_str_mv	RAMIREZ, Juan Carlos; et al. Analytical Validation of Quantitative Real-Time PCR Methods for Quantification of Trypanosoma cruzi DNA in Blood Samples from Chagas Disease Patients. J Mol Diagn., v.17, n.5, p.605-615, Sept. 2015.
dc.identifier.uri.fl_str_mv	https://www.arca.fiocruz.br/handle/icict/12920
dc.identifier.issn.none.fl_str_mv	1525-1578
dc.identifier.doi.none.fl_str_mv	10.1016/j.jmoldx.2015.04.010
dc.identifier.eissn.none.fl_str_mv	1943-7811
identifier_str_mv	RAMIREZ, Juan Carlos; et al. Analytical Validation of Quantitative Real-Time PCR Methods for Quantification of Trypanosoma cruzi DNA in Blood Samples from Chagas Disease Patients. J Mol Diagn., v.17, n.5, p.605-615, Sept. 2015. 1525-1578 10.1016/j.jmoldx.2015.04.010 1943-7811
url	https://www.arca.fiocruz.br/handle/icict/12920
dc.language.iso.fl_str_mv	eng
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dc.publisher.none.fl_str_mv	Elsevier
publisher.none.fl_str_mv	Elsevier
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repository.name.fl_str_mv	Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
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Analytical Validation of Quantitative Real-Time PCR Methods for Quantification of Trypanosoma cruzi DNA in Blood Samples from Chagas Disease Patients

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