Purification and Characterization of BmooAi: A New Toxin from Bothrops moojeni Snake Venom That Inhibits Platelet Aggregation

Detalhes bibliográficos
Autor(a) principal: Queiroz, Mayara Ribeiro de
Data de Publicação: 2014
Outros Autores: Mamede, Carla Cristine N., Morais, Nadia Cristina G. de, Fonseca, Kelly Cortes, Sousa, Bruna Barbosa de, Migliorini, Thaís M., Pereira, Déborah Fernanda C., Stanziola, Leonilda, Calderon, Leonardo A., Silva, Rodrigo Simões, Soares, Andreimar Martins, Oliveira, Fábio de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/13861
http://dx.doi.org/10.1155/2014/920942
Resumo: Universidade Federal de Uberlândia. Instituto de Genética e Bioquímica. Uberlândia, MG, Brazil / Instituto Nacional de Ciência e Tecnologia em Nano-Biofarmacêutica. Belo Horizonte, MG, Brazil.
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spelling Queiroz, Mayara Ribeiro deMamede, Carla Cristine N.Morais, Nadia Cristina G. deFonseca, Kelly CortesSousa, Bruna Barbosa deMigliorini, Thaís M.Pereira, Déborah Fernanda C.Stanziola, LeonildaCalderon, Leonardo A.Silva, Rodrigo SimõesSoares, Andreimar MartinsOliveira, Fábio de2016-04-19T13:40:24Z2016-04-19T13:40:24Z2014CALDERON, L. A. et al. Purification and Characterization of BmooAi: A New Toxin from Bothrops moojeni Snake Venom That Inhibits Platelet Aggregation. BioMed Research International, v. 2014, p. 1-7, 2014.https://www.arca.fiocruz.br/handle/icict/13861http://dx.doi.org/10.1155/2014/920942engBioMed Research InternationalPurification and Characterization of BmooAi: A New Toxin from Bothrops moojeni Snake Venom That Inhibits Platelet Aggregationinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de Uberlândia. Instituto de Genética e Bioquímica. Uberlândia, MG, Brazil / Instituto Nacional de Ciência e Tecnologia em Nano-Biofarmacêutica. Belo Horizonte, MG, Brazil.Universidade Federal de Uberlândia. Instituto de Genética e Bioquímica. Uberlândia, MG, Brazil / Instituto Nacional de Ciência e Tecnologia em Nano-Biofarmacêutica. Belo Horizonte, MG, Brazil.Universidade Federal de Uberlândia. Instituto de Genética e Bioquímica. Uberlândia, MG, Brazil / Instituto Nacional de Ciência e Tecnologia em Nano-Biofarmacêutica. Belo Horizonte, MG, Brazil.Universidade Federal de Uberlândia. Instituto de Genética e Bioquímica. Uberlândia, MG, Brazil / Instituto Nacional de Ciência e Tecnologia em Nano-Biofarmacêutica. Belo Horizonte, MG, Brazil.Universidade Federal de Uberlândia. Instituto de Genética e Bioquímica. Uberlândia, MG, Brazil./ Instituto Nacional de Ciência e Tecnologia em Nano-Biofarmacêutica. Belo Horizonte, MG, Brazil.Universidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Uberlândia, MG, Brazil.Universidade Federal de Uberlândia. Instituto de Genética e Bioquímica. Uberlândia, MG, Brazil.Instituto Nacional de Ciência e Tecnologia em Nano-Biofarmacêutica. Belo Horizonte, MG, Brazil / Universidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Uberlândia, MG, Brazil.Fundação Oswaldo Cruz. Centro de Estudos de Biomoléculas Aplicadas à Saúde e Núcleo de Saúde. Porto Velho, RO, Brazil / Universidade Federal de Rondônia. Porto Velho, RO, Brazil.Fundação Oswaldo Cruz. Centro de Estudos de Biomoléculas Aplicadas à Saúde e Núcleo de Saúde. Porto Velho, RO, Brazil / Universidade Federal de Rondônia. Porto Velho, RO, Brazil.Fundação Oswaldo Cruz. Centro de Estudos de Biomoléculas Aplicadas à Saúde e Núcleo de Saúde. Porto Velho, RO, Brazil / Universidade Federal de Rondônia. Porto Velho, RO, Brazil.Instituto Nacional de Ciência e Tecnologia em Nano-Biofarmacêutica. Belo Horizonte, MG, Brazil / Universidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Uberlândia, MG, Brazil.In this paper, we describe the purification/characterization of BmooAi, a new toxin from Bothrops moojeni that inhibits platelet aggregation. The purification of BmooAi was carried out through three chromatographic steps (ion-exchange on a DEAE-Sephacel column, molecular exclusion on a Sephadex G-75 column, and reverse-phase HPLC chromatography on a C2/C18 column). BmooAi was homogeneous by SDS-PAGE and shown to be a single-chain protein of 15,000 Da. BmooAi was analysed by MALDI-TOF Spectrometry and revealed two major components with molecular masses 7824.4 and 7409.2 as well as a trace of protein with a molecular mass of 15,237.4 Da. Sequencing of BmooAi by Edman degradation showed two amino acid sequences: IRDFDPLTNAPENTA and ETEEGAEEGTQ, which revealed no homology to any known toxin from snake venom. BmooAi showed a rather specific inhibitory effect on platelet aggregation induced by collagen, adenosine diphosphate, or epinephrine in human platelet-rich plasma in a dose-dependent manner, whereas it had little or no effect on platelet aggregation induced by ristocetin. The effect on platelet aggregation induced by BmooAi remained active even when heated to 100∘C. BmooAi could be of medical interest as a new tool for the development of novel therapeutic agents for the prevention and treatment of thrombotic disorders.BmooAiNew ToxinBothrops moojeniSnake VenomPlatelet Aggregationinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALPurification and Characterization of BmooAi.pdfPurification and Characterization of BmooAi.pdfapplication/pdf1548419https://www.arca.fiocruz.br/bitstream/icict/13861/2/Purification%20and%20Characterization%20of%20BmooAi.pdfd761c248da6e625f6426c3b0b70fa3eaMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/13861/3/license.txt5a560609d32a3863062d77ff32785d58MD53TEXTPurification and Characterization of BmooAi.pdf.txtPurification and Characterization of BmooAi.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Purification and Characterization of BmooAi: A New Toxin from Bothrops moojeni Snake Venom That Inhibits Platelet Aggregation
title Purification and Characterization of BmooAi: A New Toxin from Bothrops moojeni Snake Venom That Inhibits Platelet Aggregation
spellingShingle Purification and Characterization of BmooAi: A New Toxin from Bothrops moojeni Snake Venom That Inhibits Platelet Aggregation
Queiroz, Mayara Ribeiro de
BmooAi
New Toxin
Bothrops moojeni
Snake Venom
Platelet Aggregation
title_short Purification and Characterization of BmooAi: A New Toxin from Bothrops moojeni Snake Venom That Inhibits Platelet Aggregation
title_full Purification and Characterization of BmooAi: A New Toxin from Bothrops moojeni Snake Venom That Inhibits Platelet Aggregation
title_fullStr Purification and Characterization of BmooAi: A New Toxin from Bothrops moojeni Snake Venom That Inhibits Platelet Aggregation
title_full_unstemmed Purification and Characterization of BmooAi: A New Toxin from Bothrops moojeni Snake Venom That Inhibits Platelet Aggregation
title_sort Purification and Characterization of BmooAi: A New Toxin from Bothrops moojeni Snake Venom That Inhibits Platelet Aggregation
author Queiroz, Mayara Ribeiro de
author_facet Queiroz, Mayara Ribeiro de
Mamede, Carla Cristine N.
Morais, Nadia Cristina G. de
Fonseca, Kelly Cortes
Sousa, Bruna Barbosa de
Migliorini, Thaís M.
Pereira, Déborah Fernanda C.
Stanziola, Leonilda
Calderon, Leonardo A.
Silva, Rodrigo Simões
Soares, Andreimar Martins
Oliveira, Fábio de
author_role author
author2 Mamede, Carla Cristine N.
Morais, Nadia Cristina G. de
Fonseca, Kelly Cortes
Sousa, Bruna Barbosa de
Migliorini, Thaís M.
Pereira, Déborah Fernanda C.
Stanziola, Leonilda
Calderon, Leonardo A.
Silva, Rodrigo Simões
Soares, Andreimar Martins
Oliveira, Fábio de
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Queiroz, Mayara Ribeiro de
Mamede, Carla Cristine N.
Morais, Nadia Cristina G. de
Fonseca, Kelly Cortes
Sousa, Bruna Barbosa de
Migliorini, Thaís M.
Pereira, Déborah Fernanda C.
Stanziola, Leonilda
Calderon, Leonardo A.
Silva, Rodrigo Simões
Soares, Andreimar Martins
Oliveira, Fábio de
dc.subject.en.en.fl_str_mv BmooAi
New Toxin
Bothrops moojeni
Snake Venom
Platelet Aggregation
topic BmooAi
New Toxin
Bothrops moojeni
Snake Venom
Platelet Aggregation
description Universidade Federal de Uberlândia. Instituto de Genética e Bioquímica. Uberlândia, MG, Brazil / Instituto Nacional de Ciência e Tecnologia em Nano-Biofarmacêutica. Belo Horizonte, MG, Brazil.
publishDate 2014
dc.date.issued.fl_str_mv 2014
dc.date.accessioned.fl_str_mv 2016-04-19T13:40:24Z
dc.date.available.fl_str_mv 2016-04-19T13:40:24Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv CALDERON, L. A. et al. Purification and Characterization of BmooAi: A New Toxin from Bothrops moojeni Snake Venom That Inhibits Platelet Aggregation. BioMed Research International, v. 2014, p. 1-7, 2014.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/13861
dc.identifier.doi.none.fl_str_mv http://dx.doi.org/10.1155/2014/920942
identifier_str_mv CALDERON, L. A. et al. Purification and Characterization of BmooAi: A New Toxin from Bothrops moojeni Snake Venom That Inhibits Platelet Aggregation. BioMed Research International, v. 2014, p. 1-7, 2014.
url https://www.arca.fiocruz.br/handle/icict/13861
http://dx.doi.org/10.1155/2014/920942
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv BioMed Research International
publisher.none.fl_str_mv BioMed Research International
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
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collection Repositório Institucional da FIOCRUZ (ARCA)
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