Long-term exposure of Aedes aegypti to Bacillus thuringiensis svar. israelensis did not involve altered susceptibility to this microbial larvicide or to other control agents

Detalhes bibliográficos
Autor(a) principal: Carvalho, Karine da Silva
Data de Publicação: 2018
Outros Autores: Crespo, Mônica Maria, Araújo, Ana Paula, Silva, Renata Santana da, Melo-Santos, Maria Alice Varjal de, Oliveira, Cláudia Maria Fontes de, Silva-Filha, Maria Helena Neves Lobo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/32260
Resumo: Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia. Recife, PE, Brasil.
id CRUZ_add76149be74ad6ae3c27ac988abaf83
oai_identifier_str oai:www.arca.fiocruz.br:icict/32260
network_acronym_str CRUZ
network_name_str Repositório Institucional da FIOCRUZ (ARCA)
repository_id_str 2135
spelling Carvalho, Karine da SilvaCrespo, Mônica MariaAraújo, Ana PaulaSilva, Renata Santana daMelo-Santos, Maria Alice Varjal deOliveira, Cláudia Maria Fontes deSilva-Filha, Maria Helena Neves Lobo2019-03-28T12:22:38Z2019-03-28T12:22:38Z2018CARVALHO, K. da S. et al. Long-Term Exposure of Aedes Aegypti to Bacillus Thuringiensis Svar. Israelensis Did Not Involve Altered Susceptibility to This Microbial Larvicide or to Other Control Agents. Parasites & Vectors, v. 11, n. 1, p. 673, 29 dez. 2018.1756-3305https://www.arca.fiocruz.br/handle/icict/3226010.1186/s13071-018-3246-1engLong-term exposure of Aedes aegypti to Bacillus thuringiensis svar. israelensis did not involve altered susceptibility to this microbial larvicide or to other control agentsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia. Recife, PE, Brasil.Background: Bacillus thuringiensis svar. israelensis (Bti) is an effective and safe biolarvicide to control Aedes aegypti. Its mode of action based on four protoxins disfavors resistance; however, control in endemic areas that display high mosquito infestation throughout the year requires continuous larvicide applications, which imposes a strong selection pressure. Therefore, this study aimed to investigate the effects of an intensive Bti exposure on an Ae. aegypti strain (RecBti), regarding its susceptibility to Bti and two of its protoxins tested individually, to other control agents temephos and diflubenzuron, and its profile of detoxifying enzymes. Methods: The RecBti strain was established using a large egg sample (10,000) from Recife city (Brazil) and more than 290,000 larvae were subjected to Bti throughout 30 generations. Larvae susceptibility to larvicides and the activity of detoxifying enzymes were determined by bioassays and catalytic assays, respectively. The Rockefeller strain was the reference used for these evaluations. Results: Bti exposure yielded an average of 74% mortality at each generation. Larvae assessed in seven time points throughout the 30 generations were susceptible to Bti crystal (resistance ratio RR ≤ 2.8) and to its individual toxins Cry11Aa and Cry4Ba (RR ≤ 4.1). Early signs of altered susceptibility to Cry11Aa were detected in the last evaluations, suggesting that this toxin was a marker of the selection pressure imposed. RecBti larvae were also susceptible (RR ≤ 1.6) to the other control agents, temephos and diflubenzuron. The activity of the detoxifying enzymes α- and β-esterases, glutathione-S-transferases and mixed-function oxidases was classified as unaltered in larvae from two generations (F19 and F25), except for a β-esterases increase in F25. Conclusions: Prolonged exposure of Ae. aegypti larvae to Bti did not evolve into resistance to the crystal, and no cross-resistance with temephos and diflubenzuron were recorded, which supports their sustainable use with Bti for integrated control practices. The unaltered activity of most detoxifying enzymes suggests that they might not play a major role in the metabolism of Bti toxins, therefore resistance by this mechanism is unlikely to occur. This study also highlights the need to establish suitable criteria to classify the status of larval susceptibility/resistance.Cry11AaCry4BaTemephosDiflubenzuronDetoxifying enzymesResistanceAedes / efeitos de drogasAnimaisBacillus thuringiensis / crescimento e desenvolvimentoBacillus thuringiensis / patogenicidadeProteínas Bacterianas / FarmacologiaBrasilEndotoxinas / farmacologiaProteínas Hemolisinas / FarmacologiaResistência a inseticidasInseticidas / farmacologiaLarva / efeitos de drogasControle de Mosquitos / métodosControle de Pragas Biológico / métodosinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83094https://www.arca.fiocruz.br/bitstream/icict/32260/1/license.txtee3692f7b0b97d5638099ab94397d05fMD51ORIGINALLong-term exposure of Aedes aegypti to Bacillus thuringiensis svar. israelensis did not involve altered susceptibility to this microbial larvicide or to other control agents.pdfLong-term exposure of Aedes aegypti to Bacillus thuringiensis svar. israelensis did not involve altered susceptibility to this microbial larvicide or to other control agents.pdfapplication/pdf874047https://www.arca.fiocruz.br/bitstream/icict/32260/2/Long-term%20exposure%20of%20Aedes%20aegypti%20to%20Bacillus%20thuringiensis%20svar.%20israelensis%20did%20not%20involve%20altered%20susceptibility%20to%20this%20microbial%20larvicide%20or%20to%20other%20control%20agents.pdf6846d7cf8ab469599c9804be16c4f20bMD52TEXTLong-term exposure of Aedes aegypti to Bacillus thuringiensis svar. israelensis did not involve altered susceptibility to this microbial larvicide or to other control agents.pdf.txtLong-term exposure of Aedes aegypti to Bacillus thuringiensis svar. israelensis did not involve altered susceptibility to this microbial larvicide or to other control agents.pdf.txtExtracted texttext/plain59043https://www.arca.fiocruz.br/bitstream/icict/32260/3/Long-term%20exposure%20of%20Aedes%20aegypti%20to%20Bacillus%20thuringiensis%20svar.%20israelensis%20did%20not%20involve%20altered%20susceptibility%20to%20this%20microbial%20larvicide%20or%20to%20other%20control%20agents.pdf.txt4c1bbf98b80dd1e527ad79d47daeba67MD53icict/322602019-04-04 02:10:52.524oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352019-04-04T05:10:52Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Long-term exposure of Aedes aegypti to Bacillus thuringiensis svar. israelensis did not involve altered susceptibility to this microbial larvicide or to other control agents
title Long-term exposure of Aedes aegypti to Bacillus thuringiensis svar. israelensis did not involve altered susceptibility to this microbial larvicide or to other control agents
spellingShingle Long-term exposure of Aedes aegypti to Bacillus thuringiensis svar. israelensis did not involve altered susceptibility to this microbial larvicide or to other control agents
Carvalho, Karine da Silva
Cry11Aa
Cry4Ba
Temephos
Diflubenzuron
Detoxifying enzymes
Resistance
Aedes / efeitos de drogas
Animais
Bacillus thuringiensis / crescimento e desenvolvimento
Bacillus thuringiensis / patogenicidade
Proteínas Bacterianas / Farmacologia
Brasil
Endotoxinas / farmacologia
Proteínas Hemolisinas / Farmacologia
Resistência a inseticidas
Inseticidas / farmacologia
Larva / efeitos de drogas
Controle de Mosquitos / métodos
Controle de Pragas Biológico / métodos
title_short Long-term exposure of Aedes aegypti to Bacillus thuringiensis svar. israelensis did not involve altered susceptibility to this microbial larvicide or to other control agents
title_full Long-term exposure of Aedes aegypti to Bacillus thuringiensis svar. israelensis did not involve altered susceptibility to this microbial larvicide or to other control agents
title_fullStr Long-term exposure of Aedes aegypti to Bacillus thuringiensis svar. israelensis did not involve altered susceptibility to this microbial larvicide or to other control agents
title_full_unstemmed Long-term exposure of Aedes aegypti to Bacillus thuringiensis svar. israelensis did not involve altered susceptibility to this microbial larvicide or to other control agents
title_sort Long-term exposure of Aedes aegypti to Bacillus thuringiensis svar. israelensis did not involve altered susceptibility to this microbial larvicide or to other control agents
author Carvalho, Karine da Silva
author_facet Carvalho, Karine da Silva
Crespo, Mônica Maria
Araújo, Ana Paula
Silva, Renata Santana da
Melo-Santos, Maria Alice Varjal de
Oliveira, Cláudia Maria Fontes de
Silva-Filha, Maria Helena Neves Lobo
author_role author
author2 Crespo, Mônica Maria
Araújo, Ana Paula
Silva, Renata Santana da
Melo-Santos, Maria Alice Varjal de
Oliveira, Cláudia Maria Fontes de
Silva-Filha, Maria Helena Neves Lobo
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Carvalho, Karine da Silva
Crespo, Mônica Maria
Araújo, Ana Paula
Silva, Renata Santana da
Melo-Santos, Maria Alice Varjal de
Oliveira, Cláudia Maria Fontes de
Silva-Filha, Maria Helena Neves Lobo
dc.subject.en.pt_BR.fl_str_mv Cry11Aa
Cry4Ba
Temephos
Diflubenzuron
Detoxifying enzymes
Resistance
topic Cry11Aa
Cry4Ba
Temephos
Diflubenzuron
Detoxifying enzymes
Resistance
Aedes / efeitos de drogas
Animais
Bacillus thuringiensis / crescimento e desenvolvimento
Bacillus thuringiensis / patogenicidade
Proteínas Bacterianas / Farmacologia
Brasil
Endotoxinas / farmacologia
Proteínas Hemolisinas / Farmacologia
Resistência a inseticidas
Inseticidas / farmacologia
Larva / efeitos de drogas
Controle de Mosquitos / métodos
Controle de Pragas Biológico / métodos
dc.subject.decs.pt_BR.fl_str_mv Aedes / efeitos de drogas
Animais
Bacillus thuringiensis / crescimento e desenvolvimento
Bacillus thuringiensis / patogenicidade
Proteínas Bacterianas / Farmacologia
Brasil
Endotoxinas / farmacologia
Proteínas Hemolisinas / Farmacologia
Resistência a inseticidas
Inseticidas / farmacologia
Larva / efeitos de drogas
Controle de Mosquitos / métodos
Controle de Pragas Biológico / métodos
description Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia. Recife, PE, Brasil.
publishDate 2018
dc.date.issued.fl_str_mv 2018
dc.date.accessioned.fl_str_mv 2019-03-28T12:22:38Z
dc.date.available.fl_str_mv 2019-03-28T12:22:38Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv CARVALHO, K. da S. et al. Long-Term Exposure of Aedes Aegypti to Bacillus Thuringiensis Svar. Israelensis Did Not Involve Altered Susceptibility to This Microbial Larvicide or to Other Control Agents. Parasites & Vectors, v. 11, n. 1, p. 673, 29 dez. 2018.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/32260
dc.identifier.issn.pt_BR.fl_str_mv 1756-3305
dc.identifier.doi.none.fl_str_mv 10.1186/s13071-018-3246-1
identifier_str_mv CARVALHO, K. da S. et al. Long-Term Exposure of Aedes Aegypti to Bacillus Thuringiensis Svar. Israelensis Did Not Involve Altered Susceptibility to This Microbial Larvicide or to Other Control Agents. Parasites & Vectors, v. 11, n. 1, p. 673, 29 dez. 2018.
1756-3305
10.1186/s13071-018-3246-1
url https://www.arca.fiocruz.br/handle/icict/32260
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/32260/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/32260/2/Long-term%20exposure%20of%20Aedes%20aegypti%20to%20Bacillus%20thuringiensis%20svar.%20israelensis%20did%20not%20involve%20altered%20susceptibility%20to%20this%20microbial%20larvicide%20or%20to%20other%20control%20agents.pdf
https://www.arca.fiocruz.br/bitstream/icict/32260/3/Long-term%20exposure%20of%20Aedes%20aegypti%20to%20Bacillus%20thuringiensis%20svar.%20israelensis%20did%20not%20involve%20altered%20susceptibility%20to%20this%20microbial%20larvicide%20or%20to%20other%20control%20agents.pdf.txt
bitstream.checksum.fl_str_mv ee3692f7b0b97d5638099ab94397d05f
6846d7cf8ab469599c9804be16c4f20b
4c1bbf98b80dd1e527ad79d47daeba67
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
_version_ 1798324772604477440

Registros relacionados