Molecular typing and in vitro antifungal susceptibility of Cryptococcus spp from patients in Midwest Brazil

Detalhes bibliográficos
Autor(a) principal: Favalessa, Olivia Cometti
Data de Publicação: 2014
Outros Autores: Paula, Daphine Ariadne Jesus de, Dutra, Valéria, Nakazato, Luciano, Tadano, Tomoko, Lazera, Márcia dos Santos, Wanke, Bodo, Trilles, Luciana, Szeszs, Maria Walderez, Silva, Dayane, Hahn, Rosane Christine
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/11647
Resumo: Universidade Federal de Mato Grosso. Faculdade de Medicina. Laboratório de Micologia. Cuiabá, MT, Brasil.
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spelling Favalessa, Olivia ComettiPaula, Daphine Ariadne Jesus deDutra, ValériaNakazato, LucianoTadano, TomokoLazera, Márcia dos SantosWanke, BodoTrilles, LucianaSzeszs, Maria WalderezSilva, DayaneHahn, Rosane Christine2015-08-25T16:40:17Z2015-08-25T16:40:17Z2014FAVALESSA, Olivia Cometti et al. Molecular typing and in vitro antifungal susceptibility of Cryptococcus spp from patients in Midwest Brazil. Journal of Infection in Developing Countries, v. 8, n. 8, p.1037-1043, 2014.1972-2680https://www.arca.fiocruz.br/handle/icict/1164710.3855/jidc.44462036-6590engOpen Learning on Enteric PathogensMolecular typing and in vitro antifungal susceptibility of Cryptococcus spp from patients in Midwest Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de Mato Grosso. Faculdade de Medicina. Laboratório de Micologia. Cuiabá, MT, Brasil.Universidade Federal de Mato Grosso. Laboratório de Microbiologia e Biologia Molecular Veterinária. Cuiabá, MT, Brasil.Universidade Federal de Mato Grosso. Laboratório de Microbiologia e Biologia Molecular Veterinária. Cuiabá, MT, Brasil.Universidade Federal de Mato Grosso. Laboratório de Microbiologia e Biologia Molecular Veterinária. Cuiabá, MT, Brasil.Universidade Federal de Mato Grosso. Hospital Universitário Júlio Müller. Cuiabá, MT, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.Instituto Adolfo Lutz. Seção de Micologia. São Paulo, SP, Brasil.Instituto Adolfo Lutz. Seção de Micologia. São Paulo, SP, Brasil.Universidade Federal de Mato Grosso. Faculdade de Medicina. Laboratório de Micologia. Cuiabá, MT, Brasil / Universidade Federal de Mato Grosso. Hospital Universitário Júlio Müller. Cuiabá, MT, Brasil.Introduction: Cryptococcosis is a systemic fungal infection that affects humans and animals, mainly due to Cryptococcus neoformans and Cryptococcus gattii. Following the epidemic of acquired immunodeficiency syndrome (AIDS), fungal infections by C. neoformans have become more common among immunocompromised patients. Cryptococcus gattii has primarily been isolated as a primary pathogen in healthy hosts and occurs endemically in northern and northeastern Brazil. We to perform genotypic characterization and determine the in vitro susceptibility profile to antifungal drugs of the Cryptococcus species complex isolated from HIV-positive and HIV-negative patients attended at university hospitals in Cuiabá, MT, in the Midwestern region of Brazil. Methodology: Micromorphological features, chemotyping with canavanine-glycine-bromothymol blue (CGB) agar and genotyping by URA5-RFLP were used to identify the species. The antifungal drugs tested were amphotericin B, fluconazole, flucytosine, itraconazole and voriconazole. Minimum inhibitory concentrations (MICs) were determined according to the CLSI methodology M27-A3. Results: Analysis of samples yelded C. neoformans AFLP1/VNI (17/27, 63.0%) and C. gattii AFLP6/VGII (10/27, 37.0%). The MICs ranges for the antifungal drugs were: amphotericin B (0.5-1 mg/L), fluconazole (1-16 mg/L), flucytosine (1-16 mg/L), itraconazole (0.25-0.12 mg/L) and voriconazole (0.06-0.5 mg/L). Isolates of C. neoformans AFLP1/VNI were predominant in patients with HIV/AIDS, and C. gattii VGII in HIV-negative patients. The genotypes identified were susceptible to the antifungal drugs tested. Conclusion: It is worth emphasizing that AFLP6/VGII is a predominant genotype affecting HIV-negative individuals in Cuiabá. These findings serve as a guide concerning the molecular epidemiology of C. neoformans and C. gattii in the State of Mato Grosso.CryptococcusAntifungal agentsCryptococcus neoformansCryptococcus gattiiCryptococcusAntifúngicosCryptococcus neoformansCryptococcus gattiiinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82354https://www.arca.fiocruz.br/bitstream/icict/11647/1/license.txt8b4c200b4e10021c5683c6ccaba07169MD51ORIGINALMolecular typing and in vitro antifungal susceptibility of Cryptococcus spp from patients in Midwest Brazil.pdfMolecular typing and in vitro antifungal susceptibility of Cryptococcus spp from patients in Midwest Brazil.pdfapplication/pdf532316https://www.arca.fiocruz.br/bitstream/icict/11647/2/Molecular%20typing%20and%20in%20vitro%20antifungal%20susceptibility%20of%20Cryptococcus%20spp%20from%20patients%20in%20Midwest%20Brazil.pdf3d5faf796e096bc18c36adca72b86468MD52TEXTMolecular typing and in vitro antifungal susceptibility of Cryptococcus spp from patients in Midwest Brazil.pdf.txtMolecular typing and in vitro antifungal susceptibility of Cryptococcus spp from patients in Midwest Brazil.pdf.txtExtracted texttext/plain34689https://www.arca.fiocruz.br/bitstream/icict/11647/3/Molecular%20typing%20and%20in%20vitro%20antifungal%20susceptibility%20of%20Cryptococcus%20spp%20from%20patients%20in%20Midwest%20Brazil.pdf.txt0074ae20d17778b59e9f620c986a7470MD53icict/116472019-09-09 10:18:31.09oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352019-09-09T13:18:31Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Molecular typing and in vitro antifungal susceptibility of Cryptococcus spp from patients in Midwest Brazil
title Molecular typing and in vitro antifungal susceptibility of Cryptococcus spp from patients in Midwest Brazil
spellingShingle Molecular typing and in vitro antifungal susceptibility of Cryptococcus spp from patients in Midwest Brazil
Favalessa, Olivia Cometti
Cryptococcus
Antifungal agents
Cryptococcus neoformans
Cryptococcus gattii
Cryptococcus
Antifúngicos
Cryptococcus neoformans
Cryptococcus gattii
title_short Molecular typing and in vitro antifungal susceptibility of Cryptococcus spp from patients in Midwest Brazil
title_full Molecular typing and in vitro antifungal susceptibility of Cryptococcus spp from patients in Midwest Brazil
title_fullStr Molecular typing and in vitro antifungal susceptibility of Cryptococcus spp from patients in Midwest Brazil
title_full_unstemmed Molecular typing and in vitro antifungal susceptibility of Cryptococcus spp from patients in Midwest Brazil
title_sort Molecular typing and in vitro antifungal susceptibility of Cryptococcus spp from patients in Midwest Brazil
author Favalessa, Olivia Cometti
author_facet Favalessa, Olivia Cometti
Paula, Daphine Ariadne Jesus de
Dutra, Valéria
Nakazato, Luciano
Tadano, Tomoko
Lazera, Márcia dos Santos
Wanke, Bodo
Trilles, Luciana
Szeszs, Maria Walderez
Silva, Dayane
Hahn, Rosane Christine
author_role author
author2 Paula, Daphine Ariadne Jesus de
Dutra, Valéria
Nakazato, Luciano
Tadano, Tomoko
Lazera, Márcia dos Santos
Wanke, Bodo
Trilles, Luciana
Szeszs, Maria Walderez
Silva, Dayane
Hahn, Rosane Christine
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Favalessa, Olivia Cometti
Paula, Daphine Ariadne Jesus de
Dutra, Valéria
Nakazato, Luciano
Tadano, Tomoko
Lazera, Márcia dos Santos
Wanke, Bodo
Trilles, Luciana
Szeszs, Maria Walderez
Silva, Dayane
Hahn, Rosane Christine
dc.subject.en.pt_BR.fl_str_mv Cryptococcus
Antifungal agents
Cryptococcus neoformans
Cryptococcus gattii
topic Cryptococcus
Antifungal agents
Cryptococcus neoformans
Cryptococcus gattii
Cryptococcus
Antifúngicos
Cryptococcus neoformans
Cryptococcus gattii
dc.subject.decs.pt_BR.fl_str_mv Cryptococcus
Antifúngicos
Cryptococcus neoformans
Cryptococcus gattii
description Universidade Federal de Mato Grosso. Faculdade de Medicina. Laboratório de Micologia. Cuiabá, MT, Brasil.
publishDate 2014
dc.date.issued.fl_str_mv 2014
dc.date.accessioned.fl_str_mv 2015-08-25T16:40:17Z
dc.date.available.fl_str_mv 2015-08-25T16:40:17Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv FAVALESSA, Olivia Cometti et al. Molecular typing and in vitro antifungal susceptibility of Cryptococcus spp from patients in Midwest Brazil. Journal of Infection in Developing Countries, v. 8, n. 8, p.1037-1043, 2014.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/11647
dc.identifier.issn.none.fl_str_mv 1972-2680
dc.identifier.doi.none.fl_str_mv 10.3855/jidc.4446
dc.identifier.eissn.none.fl_str_mv 2036-6590
identifier_str_mv FAVALESSA, Olivia Cometti et al. Molecular typing and in vitro antifungal susceptibility of Cryptococcus spp from patients in Midwest Brazil. Journal of Infection in Developing Countries, v. 8, n. 8, p.1037-1043, 2014.
1972-2680
10.3855/jidc.4446
2036-6590
url https://www.arca.fiocruz.br/handle/icict/11647
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Open Learning on Enteric Pathogens
publisher.none.fl_str_mv Open Learning on Enteric Pathogens
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/11647/1/license.txt
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