A Rapid and Simple HPLC Method for Therapeutic Monitoring of Vancomycin

Detalhes bibliográficos
Autor(a) principal: Lima, Tácio de Mendonça
Data de Publicação: 2018
Outros Autores: Seba, Karine Souza, Gonçalves, José Carlos Saraiva, Cardoso, Fernando Luiz Lopes, Estrela, Rita de Cássia Elias
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/27091
Resumo: 2019-02-28
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spelling Lima, Tácio de MendonçaSeba, Karine SouzaGonçalves, José Carlos SaraivaCardoso, Fernando Luiz LopesEstrela, Rita de Cássia Elias2018-06-25T19:36:34Z2018-06-25T19:36:34Z2018LIMA, Tácio de Mendonça et al. A Rapid and Simple HPLC Method for Therapeutic Monitoring of Vancomycin. Journal of Chromatographic Science, v. 56,n . 2, p. 115–121, 2018.0021-9665https://www.arca.fiocruz.br/handle/icict/2709110.1093/chromsci/bmx089engA Rapid and Simple HPLC Method for Therapeutic Monitoring of Vancomycininfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2019-02-28Faculty of Pharmaceutical Sciences, University of São Paulo. São Paulo - SPFundação Oswaldo Cruz. Escola Nacional de Saúde Pública. Rio de Janeiro, RJ, Brasil.Faculty of Pharmacy, Federal University of Rio de Janeiro. Rio de Janeiro, RJ, Brasil.Hospital Infection Control Team, University Hospital Clementino Fraga Filho. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz.Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil.Therapeutic monitoring of the antibiotic vancomycin is important to achieve specific plasma concentration and prevent toxic effects. Several assays have been described for vancomycin determination in clinical practice, but high-performance liquid chromatography is still considered the gold standard for the quantification of vancomycin. In this study, we developed a new and rapid high-performance liquid chromatography method requiring 50 μL of plasma for the quantification of vancomycin. Acetonitrile was used for processing plasma by protein precipitation (1:2.5). Isocratic chromatographic analysis was carried out on a C18 silica-based (2.7 μm) column with the mobile phase containing 20 mM ammonium acetate/formic acid buffer (pH 4.0):methanol 88:12 (v/v). A diode array detector was used for UV detection at 240 nm. This method was validated according to the Brazilian Health Surveillance Agency legislation and International Conference on Harmonization guidelines. The measurement range was 1-100 μg/mL, analysis time was 8 min, and intermediate precision was <12%, supporting the present method as a fast, simple, and effective alternative for therapeutic monitoring of vancomycin.VancomycinChromatography, High Pressure LiquidHPLCVancomycinChromatography, High Pressure LiquidHPLCinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83099https://www.arca.fiocruz.br/bitstream/icict/27091/1/license.txt586c046dcfeef936e32f0323bb9a47c0MD51ORIGINALA Rapid and Simple HPLC Method for Therapeutic Monitoring of Vancomycin_Rita_Estrela_etal_INI_Lapclin_2018.pdfA Rapid and Simple HPLC Method for Therapeutic Monitoring of Vancomycin_Rita_Estrela_etal_INI_Lapclin_2018.pdfapplication/pdf342822https://www.arca.fiocruz.br/bitstream/icict/27091/2/A%20Rapid%20and%20Simple%20HPLC%20Method%20for%20Therapeutic%20Monitoring%20of%20Vancomycin_Rita_Estrela_etal_INI_Lapclin_2018.pdfa448653207d58056082f2a876af5465fMD52TEXTA Rapid and Simple HPLC Method for Therapeutic Monitoring of Vancomycin_Rita_Estrela_etal_INI_Lapclin_2018.pdf.txtA Rapid and Simple HPLC Method for Therapeutic Monitoring of Vancomycin_Rita_Estrela_etal_INI_Lapclin_2018.pdf.txtExtracted texttext/plain30714https://www.arca.fiocruz.br/bitstream/icict/27091/3/A%20Rapid%20and%20Simple%20HPLC%20Method%20for%20Therapeutic%20Monitoring%20of%20Vancomycin_Rita_Estrela_etal_INI_Lapclin_2018.pdf.txt95103b170a0ad8d385460937ea904184MD53icict/270912021-03-24 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dc.title.pt_BR.fl_str_mv A Rapid and Simple HPLC Method for Therapeutic Monitoring of Vancomycin
title A Rapid and Simple HPLC Method for Therapeutic Monitoring of Vancomycin
spellingShingle A Rapid and Simple HPLC Method for Therapeutic Monitoring of Vancomycin
Lima, Tácio de Mendonça
Vancomycin
Chromatography, High Pressure Liquid
HPLC
Vancomycin
Chromatography, High Pressure Liquid
HPLC
title_short A Rapid and Simple HPLC Method for Therapeutic Monitoring of Vancomycin
title_full A Rapid and Simple HPLC Method for Therapeutic Monitoring of Vancomycin
title_fullStr A Rapid and Simple HPLC Method for Therapeutic Monitoring of Vancomycin
title_full_unstemmed A Rapid and Simple HPLC Method for Therapeutic Monitoring of Vancomycin
title_sort A Rapid and Simple HPLC Method for Therapeutic Monitoring of Vancomycin
author Lima, Tácio de Mendonça
author_facet Lima, Tácio de Mendonça
Seba, Karine Souza
Gonçalves, José Carlos Saraiva
Cardoso, Fernando Luiz Lopes
Estrela, Rita de Cássia Elias
author_role author
author2 Seba, Karine Souza
Gonçalves, José Carlos Saraiva
Cardoso, Fernando Luiz Lopes
Estrela, Rita de Cássia Elias
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Lima, Tácio de Mendonça
Seba, Karine Souza
Gonçalves, José Carlos Saraiva
Cardoso, Fernando Luiz Lopes
Estrela, Rita de Cássia Elias
dc.subject.en.pt_BR.fl_str_mv Vancomycin
Chromatography, High Pressure Liquid
HPLC
topic Vancomycin
Chromatography, High Pressure Liquid
HPLC
Vancomycin
Chromatography, High Pressure Liquid
HPLC
dc.subject.decs.pt_BR.fl_str_mv Vancomycin
Chromatography, High Pressure Liquid
HPLC
description 2019-02-28
publishDate 2018
dc.date.accessioned.fl_str_mv 2018-06-25T19:36:34Z
dc.date.available.fl_str_mv 2018-06-25T19:36:34Z
dc.date.issued.fl_str_mv 2018
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv LIMA, Tácio de Mendonça et al. A Rapid and Simple HPLC Method for Therapeutic Monitoring of Vancomycin. Journal of Chromatographic Science, v. 56,n . 2, p. 115–121, 2018.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/27091
dc.identifier.issn.pt_BR.fl_str_mv 0021-9665
dc.identifier.doi.none.fl_str_mv 10.1093/chromsci/bmx089
identifier_str_mv LIMA, Tácio de Mendonça et al. A Rapid and Simple HPLC Method for Therapeutic Monitoring of Vancomycin. Journal of Chromatographic Science, v. 56,n . 2, p. 115–121, 2018.
0021-9665
10.1093/chromsci/bmx089
url https://www.arca.fiocruz.br/handle/icict/27091
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/27091/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/27091/2/A%20Rapid%20and%20Simple%20HPLC%20Method%20for%20Therapeutic%20Monitoring%20of%20Vancomycin_Rita_Estrela_etal_INI_Lapclin_2018.pdf
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
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