Transcriptome-based molecular systematics: Rhodnius montenegrensis (Triatominae) and its position within the Rhodnius prolixus–Rhodnius robustus cryptic–species complex

Detalhes bibliográficos
Autor(a) principal: Brito, Raíssa Nogueira de
Data de Publicação: 2019
Outros Autores: Geraldo, Juliana Assis, Monteiro, Fernando A., Lazoski, Cristiano, Souza, Rita Cassia Moreira de, Franch, Fernando Abad
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/33586
Resumo: Fundação Oswaldo Cruz. Instituto René Rachou. Grupo de Triatomíneos. Belo Horizonte, MG, Brasil.
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spelling Brito, Raíssa Nogueira deGeraldo, Juliana AssisMonteiro, Fernando A.Lazoski, CristianoSouza, Rita Cassia Moreira deFranch, Fernando Abad2019-06-19T16:37:02Z2019-06-19T16:37:02Z2019BRITO, Raíssa Nogueira de et al. Transcriptome-based molecular systematics: Rhodnius montenegrensis (Triatominae) and its position within the Rhodnius prolixus–Rhodnius robustus cryptic–species complex. Parasites & Vectors, v. 12, p. 1-16, 2019.1756-3305https://www.arca.fiocruz.br/handle/icict/3358610.1186/s13071-019-3558-9engBMC (part of Springer Nature)RhodniusTriatominiosSistema molecularRhodniusTriatominaeMolecular systematicsTranscriptomicsTranscriptome-based molecular systematics: Rhodnius montenegrensis (Triatominae) and its position within the Rhodnius prolixus–Rhodnius robustus cryptic–species complexinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto René Rachou. Grupo de Triatomíneos. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Programa Interunidades de Pós Graduação em Bioinformática. Belo Horizonte, MG, Brasil / Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Epidemiologia e Sistemática Molecular. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto René Rachou. Grupo de Triatomíneos. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Instituto René Rachou. Grupo de Triatomíneos. Belo Horizonte, MG, Brasil / Universidade de Brasília. Faculdade de Medicina. Núcleo de Medicina Tropical. Programa de Pós Graduação em Medicina Tropical. Brasilia, DF, Brasil.Background: Rhodnius montenegrensis (Triatominae), a potential vector of Chagas disease, was described after R. robustus-like bugs from southwestern Amazonia. Mitochondrial cytb sequence near-identity with sympatric R. robustus (genotype II) raised doubts about the taxonomic status of R. montenegrensis, but comparative studies have reported fairly clear morphological and genetic differences between R. montenegrensis and laboratory stocks identified as R. robustus. Here, we use a transcriptome-based approach to investigate this apparent paradox. Results: We retrieved publicly-available transcriptome sequence-reads from R. montenegrensis and from the R. robustus stocks used as the taxonomic benchmark in comparative studies. We (i) aligned transcriptome sequence-reads to mitochondrial (cytb) and nuclear (ITS2, D2-28S and AmpG) query sequences (47 overall) from members of the R. prolixus–R. robustus cryptic–species complex and related taxa; (ii) computed breadth- and depth-coverage for the 259 consensus sequences generated by these alignments; and, for each locus, (iii) appraised query sequences and full-breadth-coverage consensus sequences in terms of nucleotide-sequence polymorphism and phylogenetic relations. We found evidence confirming that R. montenegrensis and R. robustus genotype II are genetically indistinguishable and, hence, implying that they are, in all likelihood, the same species. Furthermore, we found compelling genetic evidence that the benchmark ‘R. robustus’ stocks used in R. montenegrensis description and in later transcriptome-based comparisons are in fact R. prolixus, although likely mixed to some degree with R. robustus (probably genotype II, a.k.a. R. montenegrensis). Conclusions: We illustrate how public-domain genetic/transcriptomic data can help address challenging issues in disease-vector systematics. In our case-study, taxonomic confusion apparently stemmed from the misinterpretation of sequence-data analyses and misidentification of taxonomic-benchmark stocks. More generally, and together with previous reports of mixed and/or misidentified Rhodnius spp. laboratory colonies, our results call into question the conclusions of many studies (on morphology, genetics, physiology, behavior, bionomics or interactions with microorganisms including trypanosomes) based on non-genotyped ‘R. prolixus’ or ‘R. robustus’ stocks. Correct species identification is a prerequisite for investigating the factors that underlie the physiological, behavioral or ecological differences between primary domestic vectors of Chagas disease, such as R. prolixus, and their sylvatic, medically less-relevant relatives such as R. robustus (s.l.) including R. montenegrensis.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83082https://www.arca.fiocruz.br/bitstream/icict/33586/1/license.txt9193a7c197bc67acd023525e72a03240MD51ORIGINALTranscriptome-based molecular systematics.pdfTranscriptome-based molecular systematics.pdfapplication/pdf7847495https://www.arca.fiocruz.br/bitstream/icict/33586/2/Transcriptome-based%20molecular%20systematics.pdfba243a40ce1bc0e82b441d8aff7ee742MD52TEXTTranscriptome-based molecular systematics.pdf.txtTranscriptome-based molecular systematics.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Transcriptome-based molecular systematics: Rhodnius montenegrensis (Triatominae) and its position within the Rhodnius prolixus–Rhodnius robustus cryptic–species complex
title Transcriptome-based molecular systematics: Rhodnius montenegrensis (Triatominae) and its position within the Rhodnius prolixus–Rhodnius robustus cryptic–species complex
spellingShingle Transcriptome-based molecular systematics: Rhodnius montenegrensis (Triatominae) and its position within the Rhodnius prolixus–Rhodnius robustus cryptic–species complex
Brito, Raíssa Nogueira de
Rhodnius
Triatominios
Sistema molecular
Rhodnius
Triatominae
Molecular systematics
Transcriptomics
title_short Transcriptome-based molecular systematics: Rhodnius montenegrensis (Triatominae) and its position within the Rhodnius prolixus–Rhodnius robustus cryptic–species complex
title_full Transcriptome-based molecular systematics: Rhodnius montenegrensis (Triatominae) and its position within the Rhodnius prolixus–Rhodnius robustus cryptic–species complex
title_fullStr Transcriptome-based molecular systematics: Rhodnius montenegrensis (Triatominae) and its position within the Rhodnius prolixus–Rhodnius robustus cryptic–species complex
title_full_unstemmed Transcriptome-based molecular systematics: Rhodnius montenegrensis (Triatominae) and its position within the Rhodnius prolixus–Rhodnius robustus cryptic–species complex
title_sort Transcriptome-based molecular systematics: Rhodnius montenegrensis (Triatominae) and its position within the Rhodnius prolixus–Rhodnius robustus cryptic–species complex
author Brito, Raíssa Nogueira de
author_facet Brito, Raíssa Nogueira de
Geraldo, Juliana Assis
Monteiro, Fernando A.
Lazoski, Cristiano
Souza, Rita Cassia Moreira de
Franch, Fernando Abad
author_role author
author2 Geraldo, Juliana Assis
Monteiro, Fernando A.
Lazoski, Cristiano
Souza, Rita Cassia Moreira de
Franch, Fernando Abad
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Brito, Raíssa Nogueira de
Geraldo, Juliana Assis
Monteiro, Fernando A.
Lazoski, Cristiano
Souza, Rita Cassia Moreira de
Franch, Fernando Abad
dc.subject.other.pt_BR.fl_str_mv Rhodnius
Triatominios
Sistema molecular
topic Rhodnius
Triatominios
Sistema molecular
Rhodnius
Triatominae
Molecular systematics
Transcriptomics
dc.subject.en.pt_BR.fl_str_mv Rhodnius
Triatominae
Molecular systematics
Transcriptomics
description Fundação Oswaldo Cruz. Instituto René Rachou. Grupo de Triatomíneos. Belo Horizonte, MG, Brasil.
publishDate 2019
dc.date.accessioned.fl_str_mv 2019-06-19T16:37:02Z
dc.date.available.fl_str_mv 2019-06-19T16:37:02Z
dc.date.issued.fl_str_mv 2019
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv BRITO, Raíssa Nogueira de et al. Transcriptome-based molecular systematics: Rhodnius montenegrensis (Triatominae) and its position within the Rhodnius prolixus–Rhodnius robustus cryptic–species complex. Parasites & Vectors, v. 12, p. 1-16, 2019.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/33586
dc.identifier.issn.pt_BR.fl_str_mv 1756-3305
dc.identifier.doi.none.fl_str_mv 10.1186/s13071-019-3558-9
identifier_str_mv BRITO, Raíssa Nogueira de et al. Transcriptome-based molecular systematics: Rhodnius montenegrensis (Triatominae) and its position within the Rhodnius prolixus–Rhodnius robustus cryptic–species complex. Parasites & Vectors, v. 12, p. 1-16, 2019.
1756-3305
10.1186/s13071-019-3558-9
url https://www.arca.fiocruz.br/handle/icict/33586
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv BMC (part of Springer Nature)
publisher.none.fl_str_mv BMC (part of Springer Nature)
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
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collection Repositório Institucional da FIOCRUZ (ARCA)
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