Detection of rifampin-resistant genotypes in Mycobacterium tuberculosis by reverse hybridization assay

Detalhes bibliográficos
Autor(a) principal: Maschmann, Raquel de A.
Data de Publicação: 2011
Outros Autores: Verza, Mirela, Silva, Marcia S. N., Sperhacke, Rosa Dea, Ribeiro, Marta O., Suffys, Philip Noel, Gomes, Harrison Magdinier, Tortoli, Enrico, Marcelli, Fiorella, Zaha, Arnaldo, Rossetti, Maria Lucia R.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/16192
Resumo: Fundação Estadual de Produção e Pesquisa em Saúde. Porto Alegre, RS, Brasil / Universidade Federal do Rio Grande do Sul. Centro de Biotecnologia. Programa de Pós-Graduação em Biologia Celular e Molecular. Porto Alegre, RS, Brasil.
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spelling Maschmann, Raquel de A.Verza, MirelaSilva, Marcia S. N.Sperhacke, Rosa DeaRibeiro, Marta O.Suffys, Philip NoelGomes, Harrison MagdinierTortoli, EnricoMarcelli, FiorellaZaha, ArnaldoRossetti, Maria Lucia R.2016-10-11T16:28:00Z2016-10-11T16:28:00Z2011MASCHMANN, Raquel de A. et al. Detection of rifampin-resistant genotypes in Mycobacterium tuberculosis by reverse hybridization assay. Mem. Inst. Oswaldo Cru, v.106, n.2, jan./ mar. 2011.0074-0276https://www.arca.fiocruz.br/handle/icict/1619210.1590/S0074-027620110002000041678-8060engFundação Oswaldo CruzTuberculoseResistência aos medicamentosreverter hibridizaçãoRifampinadrug resistancereverse dot blot hybridizationrifampinrpoBtuberculosisDetection of rifampin-resistant genotypes in Mycobacterium tuberculosis by reverse hybridization assayinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Estadual de Produção e Pesquisa em Saúde. Porto Alegre, RS, Brasil / Universidade Federal do Rio Grande do Sul. Centro de Biotecnologia. Programa de Pós-Graduação em Biologia Celular e Molecular. Porto Alegre, RS, Brasil.Fundação Estadual de Produção e Pesquisa em Saúde. Porto Alegre, RS, Brasil / Universidade Federal do Rio Grande do Sul. Centro de Biotecnologia. Programa de Pós-Graduação em Biologia Celular e Molecular. Porto Alegre, RS, Brasil.Fundação Estadual de Produção e Pesquisa em Saúde. Porto Alegre, RS, Brasil / Universidade Luterana do Brasil, Canoas, RS, Brasil .Universidade de Caxias do Sul. Laboratório de Pesquisa em HIV/AIDS. Caxias do Sul, RS, Brasil.Fundação Estadual de Produção e Pesquisa em Saúde. Porto Alegre, RS, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.Centro Regionale di Riferimento per i Micobatter. Ospedale di Careggi. Firenze, Italy;Centro Regionale di Riferimento per i Micobatter. Ospedale di Careggi. Firenze, Italy;Universidade Federal do Rio Grande do Sul. Centro de Biotecnologia. Programa de Pós-Graduação em Biologia Celular e Molecular. Porto Alegre, RS, Brasil..Fundação Estadual de Produção e Pesquisa em Saúde. Porto Alegre, RS, Brasil.. / Universidade Federal do Rio Grande do Sul. Centro de Biotecnologia. Programa de Pós-Graduação em Biologia Celular e Molecular. Porto Alegre, RS, Brasil / Universidade Luterana do Brasil. Canoas, RS, Brasil.We used a colorimetric reverse dot blot hybridization (CRDH) assay to detect the presence of mutations in a specific region of the rpoB gene, associated with rifampin (RIF) resistance, in a panel of 156 DNAs extracted from 103 RIF-sensitive and 53 RIF-resistant cultures of Mycobacterium tuberculosis. When compared with the antimicrobial susceptibility test (AST), the sensitivity and specificity of the CRDH were 92.3% and 98.1%, respectively. When compared with sequencing, the sensitivity and specificity of the CRDH were 90.6% and 100%, respectively. To evaluate the performance of the assay directly in clinical specimens, 30 samples from tuberculosis patients were used. For these samples, the results of the CRDH were 100% consistent with the results of the AST and sequencing. These results indicate that the rate of concordance of the CRDH is high when compared to conventional methods and sequencing data. The CRDH can be successfully applied when a rapid test is required for the identification of RIF resistance in M. tuberculosis.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/16192/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALharrison_gomes_etal_IOC-2011.pdfharrison_gomes_etal_IOC-2011.pdfapplication/pdf666041https://www.arca.fiocruz.br/bitstream/icict/16192/2/harrison_gomes_etal_IOC-2011.pdfcaa9ab5aff956b31c5d09322f9378adcMD52TEXTharrison_gomes_etal_IOC-2011.pdf.txtharrison_gomes_etal_IOC-2011.pdf.txtExtracted texttext/plain34628https://www.arca.fiocruz.br/bitstream/icict/16192/3/harrison_gomes_etal_IOC-2011.pdf.txt37f0cb019f9f7ecfc8dbdabacc4f2668MD53icict/161922018-04-02 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dc.title.pt_BR.fl_str_mv Detection of rifampin-resistant genotypes in Mycobacterium tuberculosis by reverse hybridization assay
title Detection of rifampin-resistant genotypes in Mycobacterium tuberculosis by reverse hybridization assay
spellingShingle Detection of rifampin-resistant genotypes in Mycobacterium tuberculosis by reverse hybridization assay
Maschmann, Raquel de A.
Tuberculose
Resistência aos medicamentos
reverter hibridização
Rifampina
drug resistance
reverse dot blot hybridization
rifampin
rpoB
tuberculosis
title_short Detection of rifampin-resistant genotypes in Mycobacterium tuberculosis by reverse hybridization assay
title_full Detection of rifampin-resistant genotypes in Mycobacterium tuberculosis by reverse hybridization assay
title_fullStr Detection of rifampin-resistant genotypes in Mycobacterium tuberculosis by reverse hybridization assay
title_full_unstemmed Detection of rifampin-resistant genotypes in Mycobacterium tuberculosis by reverse hybridization assay
title_sort Detection of rifampin-resistant genotypes in Mycobacterium tuberculosis by reverse hybridization assay
author Maschmann, Raquel de A.
author_facet Maschmann, Raquel de A.
Verza, Mirela
Silva, Marcia S. N.
Sperhacke, Rosa Dea
Ribeiro, Marta O.
Suffys, Philip Noel
Gomes, Harrison Magdinier
Tortoli, Enrico
Marcelli, Fiorella
Zaha, Arnaldo
Rossetti, Maria Lucia R.
author_role author
author2 Verza, Mirela
Silva, Marcia S. N.
Sperhacke, Rosa Dea
Ribeiro, Marta O.
Suffys, Philip Noel
Gomes, Harrison Magdinier
Tortoli, Enrico
Marcelli, Fiorella
Zaha, Arnaldo
Rossetti, Maria Lucia R.
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Maschmann, Raquel de A.
Verza, Mirela
Silva, Marcia S. N.
Sperhacke, Rosa Dea
Ribeiro, Marta O.
Suffys, Philip Noel
Gomes, Harrison Magdinier
Tortoli, Enrico
Marcelli, Fiorella
Zaha, Arnaldo
Rossetti, Maria Lucia R.
dc.subject.other.pt_BR.fl_str_mv Tuberculose
Resistência aos medicamentos
reverter hibridização
Rifampina
topic Tuberculose
Resistência aos medicamentos
reverter hibridização
Rifampina
drug resistance
reverse dot blot hybridization
rifampin
rpoB
tuberculosis
dc.subject.en.pt_BR.fl_str_mv drug resistance
reverse dot blot hybridization
rifampin
rpoB
tuberculosis
description Fundação Estadual de Produção e Pesquisa em Saúde. Porto Alegre, RS, Brasil / Universidade Federal do Rio Grande do Sul. Centro de Biotecnologia. Programa de Pós-Graduação em Biologia Celular e Molecular. Porto Alegre, RS, Brasil.
publishDate 2011
dc.date.issued.fl_str_mv 2011
dc.date.accessioned.fl_str_mv 2016-10-11T16:28:00Z
dc.date.available.fl_str_mv 2016-10-11T16:28:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv MASCHMANN, Raquel de A. et al. Detection of rifampin-resistant genotypes in Mycobacterium tuberculosis by reverse hybridization assay. Mem. Inst. Oswaldo Cru, v.106, n.2, jan./ mar. 2011.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/16192
dc.identifier.issn.pt_BR.fl_str_mv 0074-0276
dc.identifier.doi.none.fl_str_mv 10.1590/S0074-02762011000200004
dc.identifier.eissn.none.fl_str_mv 1678-8060
identifier_str_mv MASCHMANN, Raquel de A. et al. Detection of rifampin-resistant genotypes in Mycobacterium tuberculosis by reverse hybridization assay. Mem. Inst. Oswaldo Cru, v.106, n.2, jan./ mar. 2011.
0074-0276
10.1590/S0074-02762011000200004
1678-8060
url https://www.arca.fiocruz.br/handle/icict/16192
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz
publisher.none.fl_str_mv Fundação Oswaldo Cruz
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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https://www.arca.fiocruz.br/bitstream/icict/16192/2/harrison_gomes_etal_IOC-2011.pdf
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