Detection of rifampin-resistant genotypes in Mycobacterium tuberculosis by reverse hybridization assay
Autor(a) principal: | |
---|---|
Data de Publicação: | 2011 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
Texto Completo: | https://www.arca.fiocruz.br/handle/icict/16192 |
Resumo: | Fundação Estadual de Produção e Pesquisa em Saúde. Porto Alegre, RS, Brasil / Universidade Federal do Rio Grande do Sul. Centro de Biotecnologia. Programa de Pós-Graduação em Biologia Celular e Molecular. Porto Alegre, RS, Brasil. |
id |
CRUZ_af2b9b356a168b39400277dcef3d33e0 |
---|---|
oai_identifier_str |
oai:www.arca.fiocruz.br:icict/16192 |
network_acronym_str |
CRUZ |
network_name_str |
Repositório Institucional da FIOCRUZ (ARCA) |
repository_id_str |
2135 |
spelling |
Maschmann, Raquel de A.Verza, MirelaSilva, Marcia S. N.Sperhacke, Rosa DeaRibeiro, Marta O.Suffys, Philip NoelGomes, Harrison MagdinierTortoli, EnricoMarcelli, FiorellaZaha, ArnaldoRossetti, Maria Lucia R.2016-10-11T16:28:00Z2016-10-11T16:28:00Z2011MASCHMANN, Raquel de A. et al. Detection of rifampin-resistant genotypes in Mycobacterium tuberculosis by reverse hybridization assay. Mem. Inst. Oswaldo Cru, v.106, n.2, jan./ mar. 2011.0074-0276https://www.arca.fiocruz.br/handle/icict/1619210.1590/S0074-027620110002000041678-8060engFundação Oswaldo CruzTuberculoseResistência aos medicamentosreverter hibridizaçãoRifampinadrug resistancereverse dot blot hybridizationrifampinrpoBtuberculosisDetection of rifampin-resistant genotypes in Mycobacterium tuberculosis by reverse hybridization assayinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Estadual de Produção e Pesquisa em Saúde. Porto Alegre, RS, Brasil / Universidade Federal do Rio Grande do Sul. Centro de Biotecnologia. Programa de Pós-Graduação em Biologia Celular e Molecular. Porto Alegre, RS, Brasil.Fundação Estadual de Produção e Pesquisa em Saúde. Porto Alegre, RS, Brasil / Universidade Federal do Rio Grande do Sul. Centro de Biotecnologia. Programa de Pós-Graduação em Biologia Celular e Molecular. Porto Alegre, RS, Brasil.Fundação Estadual de Produção e Pesquisa em Saúde. Porto Alegre, RS, Brasil / Universidade Luterana do Brasil, Canoas, RS, Brasil .Universidade de Caxias do Sul. Laboratório de Pesquisa em HIV/AIDS. Caxias do Sul, RS, Brasil.Fundação Estadual de Produção e Pesquisa em Saúde. Porto Alegre, RS, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.Centro Regionale di Riferimento per i Micobatter. Ospedale di Careggi. Firenze, Italy;Centro Regionale di Riferimento per i Micobatter. Ospedale di Careggi. Firenze, Italy;Universidade Federal do Rio Grande do Sul. Centro de Biotecnologia. Programa de Pós-Graduação em Biologia Celular e Molecular. Porto Alegre, RS, Brasil..Fundação Estadual de Produção e Pesquisa em Saúde. Porto Alegre, RS, Brasil.. / Universidade Federal do Rio Grande do Sul. Centro de Biotecnologia. Programa de Pós-Graduação em Biologia Celular e Molecular. Porto Alegre, RS, Brasil / Universidade Luterana do Brasil. Canoas, RS, Brasil.We used a colorimetric reverse dot blot hybridization (CRDH) assay to detect the presence of mutations in a specific region of the rpoB gene, associated with rifampin (RIF) resistance, in a panel of 156 DNAs extracted from 103 RIF-sensitive and 53 RIF-resistant cultures of Mycobacterium tuberculosis. When compared with the antimicrobial susceptibility test (AST), the sensitivity and specificity of the CRDH were 92.3% and 98.1%, respectively. When compared with sequencing, the sensitivity and specificity of the CRDH were 90.6% and 100%, respectively. To evaluate the performance of the assay directly in clinical specimens, 30 samples from tuberculosis patients were used. For these samples, the results of the CRDH were 100% consistent with the results of the AST and sequencing. These results indicate that the rate of concordance of the CRDH is high when compared to conventional methods and sequencing data. The CRDH can be successfully applied when a rapid test is required for the identification of RIF resistance in M. tuberculosis.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/16192/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALharrison_gomes_etal_IOC-2011.pdfharrison_gomes_etal_IOC-2011.pdfapplication/pdf666041https://www.arca.fiocruz.br/bitstream/icict/16192/2/harrison_gomes_etal_IOC-2011.pdfcaa9ab5aff956b31c5d09322f9378adcMD52TEXTharrison_gomes_etal_IOC-2011.pdf.txtharrison_gomes_etal_IOC-2011.pdf.txtExtracted texttext/plain34628https://www.arca.fiocruz.br/bitstream/icict/16192/3/harrison_gomes_etal_IOC-2011.pdf.txt37f0cb019f9f7ecfc8dbdabacc4f2668MD53icict/161922018-04-02 07:58:39.488oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352018-04-02T10:58:39Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
dc.title.pt_BR.fl_str_mv |
Detection of rifampin-resistant genotypes in Mycobacterium tuberculosis by reverse hybridization assay |
title |
Detection of rifampin-resistant genotypes in Mycobacterium tuberculosis by reverse hybridization assay |
spellingShingle |
Detection of rifampin-resistant genotypes in Mycobacterium tuberculosis by reverse hybridization assay Maschmann, Raquel de A. Tuberculose Resistência aos medicamentos reverter hibridização Rifampina drug resistance reverse dot blot hybridization rifampin rpoB tuberculosis |
title_short |
Detection of rifampin-resistant genotypes in Mycobacterium tuberculosis by reverse hybridization assay |
title_full |
Detection of rifampin-resistant genotypes in Mycobacterium tuberculosis by reverse hybridization assay |
title_fullStr |
Detection of rifampin-resistant genotypes in Mycobacterium tuberculosis by reverse hybridization assay |
title_full_unstemmed |
Detection of rifampin-resistant genotypes in Mycobacterium tuberculosis by reverse hybridization assay |
title_sort |
Detection of rifampin-resistant genotypes in Mycobacterium tuberculosis by reverse hybridization assay |
author |
Maschmann, Raquel de A. |
author_facet |
Maschmann, Raquel de A. Verza, Mirela Silva, Marcia S. N. Sperhacke, Rosa Dea Ribeiro, Marta O. Suffys, Philip Noel Gomes, Harrison Magdinier Tortoli, Enrico Marcelli, Fiorella Zaha, Arnaldo Rossetti, Maria Lucia R. |
author_role |
author |
author2 |
Verza, Mirela Silva, Marcia S. N. Sperhacke, Rosa Dea Ribeiro, Marta O. Suffys, Philip Noel Gomes, Harrison Magdinier Tortoli, Enrico Marcelli, Fiorella Zaha, Arnaldo Rossetti, Maria Lucia R. |
author2_role |
author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Maschmann, Raquel de A. Verza, Mirela Silva, Marcia S. N. Sperhacke, Rosa Dea Ribeiro, Marta O. Suffys, Philip Noel Gomes, Harrison Magdinier Tortoli, Enrico Marcelli, Fiorella Zaha, Arnaldo Rossetti, Maria Lucia R. |
dc.subject.other.pt_BR.fl_str_mv |
Tuberculose Resistência aos medicamentos reverter hibridização Rifampina |
topic |
Tuberculose Resistência aos medicamentos reverter hibridização Rifampina drug resistance reverse dot blot hybridization rifampin rpoB tuberculosis |
dc.subject.en.pt_BR.fl_str_mv |
drug resistance reverse dot blot hybridization rifampin rpoB tuberculosis |
description |
Fundação Estadual de Produção e Pesquisa em Saúde. Porto Alegre, RS, Brasil / Universidade Federal do Rio Grande do Sul. Centro de Biotecnologia. Programa de Pós-Graduação em Biologia Celular e Molecular. Porto Alegre, RS, Brasil. |
publishDate |
2011 |
dc.date.issued.fl_str_mv |
2011 |
dc.date.accessioned.fl_str_mv |
2016-10-11T16:28:00Z |
dc.date.available.fl_str_mv |
2016-10-11T16:28:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
MASCHMANN, Raquel de A. et al. Detection of rifampin-resistant genotypes in Mycobacterium tuberculosis by reverse hybridization assay. Mem. Inst. Oswaldo Cru, v.106, n.2, jan./ mar. 2011. |
dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/16192 |
dc.identifier.issn.pt_BR.fl_str_mv |
0074-0276 |
dc.identifier.doi.none.fl_str_mv |
10.1590/S0074-02762011000200004 |
dc.identifier.eissn.none.fl_str_mv |
1678-8060 |
identifier_str_mv |
MASCHMANN, Raquel de A. et al. Detection of rifampin-resistant genotypes in Mycobacterium tuberculosis by reverse hybridization assay. Mem. Inst. Oswaldo Cru, v.106, n.2, jan./ mar. 2011. 0074-0276 10.1590/S0074-02762011000200004 1678-8060 |
url |
https://www.arca.fiocruz.br/handle/icict/16192 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Fundação Oswaldo Cruz |
publisher.none.fl_str_mv |
Fundação Oswaldo Cruz |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da FIOCRUZ (ARCA) instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ |
instname_str |
Fundação Oswaldo Cruz (FIOCRUZ) |
instacron_str |
FIOCRUZ |
institution |
FIOCRUZ |
reponame_str |
Repositório Institucional da FIOCRUZ (ARCA) |
collection |
Repositório Institucional da FIOCRUZ (ARCA) |
bitstream.url.fl_str_mv |
https://www.arca.fiocruz.br/bitstream/icict/16192/1/license.txt https://www.arca.fiocruz.br/bitstream/icict/16192/2/harrison_gomes_etal_IOC-2011.pdf https://www.arca.fiocruz.br/bitstream/icict/16192/3/harrison_gomes_etal_IOC-2011.pdf.txt |
bitstream.checksum.fl_str_mv |
5a560609d32a3863062d77ff32785d58 caa9ab5aff956b31c5d09322f9378adc 37f0cb019f9f7ecfc8dbdabacc4f2668 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 |
repository.name.fl_str_mv |
Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ) |
repository.mail.fl_str_mv |
repositorio.arca@fiocruz.br |
_version_ |
1813008827241463808 |