Detection of Mycobacterium leprae DNA by Polymerase Chain Reaction in the Blood and Nasal Secretion of Brazilian Household Contacts

Detalhes bibliográficos
Autor(a) principal: Almeida, Elza Carmen de
Data de Publicação: 2004
Outros Autores: Martinez, Alejandra Nóbrega, Maniero, Viviane Câmara, Sales, Ana Maria, Duppre, Nádia C., Sarno, Euzenir Nunes, Santos, Adalberto Rezende, Moraes, Milton Ozório
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/33278
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Medicina Tropical. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.
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spelling Almeida, Elza Carmen deMartinez, Alejandra NóbregaManiero, Viviane CâmaraSales, Ana MariaDuppre, Nádia C.Sarno, Euzenir NunesSantos, Adalberto RezendeMoraes, Milton Ozório2019-05-30T11:43:58Z2019-05-30T11:43:58Z2004ALMEIDA, Elza Carmen de et al. Detection of Mycobacterium leprae DNA by Polymerase Chain Reaction in the Blood and Nasal Secretion of Brazilian Household Contacts. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 99, n. 5, p. 509-512, Aug. 2004.0074-0276https://www.arca.fiocruz.br/handle/icict/3327810.1590/S0074-027620040005000091678-8060engFundação Oswaldo Cruz. Instituto Oswaldo CruzMycobacterium lepraeReação em cadeia da polimeraseContatos saudáveisDiagnóstico precoceMycobacterium lepraePolymerase chain reactionHealthy contactsEarly diagnosisDetection of Mycobacterium leprae DNA by Polymerase Chain Reaction in the Blood and Nasal Secretion of Brazilian Household Contactsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Medicina Tropical. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Medicina Tropical. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Medicina Tropical. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Medicina Tropical. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Medicina Tropical. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Medicina Tropical. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Medicina Tropical. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Medicina Tropical. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.DNA samples from blood and nasal swabs of 125 healthy household contacts was submitted to amplification by polymerase chain reaction (PCR) using a Mycobacterium leprae-specific sequence as a target for the detection of subclinical infection with M. leprae. All samples were submitted to hybridization analysis in order to exclude any false positive or negative results. Two positive samples were confirmed from blood out of 119 (1.7%) and two positive samples from nasal secretion out of 120 (1.7%). The analysis of the families with positive individuals showed that 2.5% (n = 3) of the contacts were relatives of multibacilary patients while 0.8% of the cases (n = 1) had a paucibacilary as an index case. All positive contacts were followed up and after one year none of them presented clinical signs of the disease. In spite of the PCR sensitivity to detect the presence of the M. leprae in a subclinical stage, this molecular approach did not seem to be a valuable tool to screen household contacts, since we determined a spurious association of the PCR positivity and further development of leprosy.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/33278/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALEuzenirSarno_MiltonMoraes_etal_IOC_2004.pdfEuzenirSarno_MiltonMoraes_etal_IOC_2004.pdfapplication/pdf26414https://www.arca.fiocruz.br/bitstream/icict/33278/2/EuzenirSarno_MiltonMoraes_etal_IOC_2004.pdfc8d8685442efc65a4cb5480a0bd9378aMD52TEXTEuzenirSarno_MiltonMoraes_etal_IOC_2004.pdf.txtEuzenirSarno_MiltonMoraes_etal_IOC_2004.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Detection of Mycobacterium leprae DNA by Polymerase Chain Reaction in the Blood and Nasal Secretion of Brazilian Household Contacts
title Detection of Mycobacterium leprae DNA by Polymerase Chain Reaction in the Blood and Nasal Secretion of Brazilian Household Contacts
spellingShingle Detection of Mycobacterium leprae DNA by Polymerase Chain Reaction in the Blood and Nasal Secretion of Brazilian Household Contacts
Almeida, Elza Carmen de
Mycobacterium leprae
Reação em cadeia da polimerase
Contatos saudáveis
Diagnóstico precoce
Mycobacterium leprae
Polymerase chain reaction
Healthy contacts
Early diagnosis
title_short Detection of Mycobacterium leprae DNA by Polymerase Chain Reaction in the Blood and Nasal Secretion of Brazilian Household Contacts
title_full Detection of Mycobacterium leprae DNA by Polymerase Chain Reaction in the Blood and Nasal Secretion of Brazilian Household Contacts
title_fullStr Detection of Mycobacterium leprae DNA by Polymerase Chain Reaction in the Blood and Nasal Secretion of Brazilian Household Contacts
title_full_unstemmed Detection of Mycobacterium leprae DNA by Polymerase Chain Reaction in the Blood and Nasal Secretion of Brazilian Household Contacts
title_sort Detection of Mycobacterium leprae DNA by Polymerase Chain Reaction in the Blood and Nasal Secretion of Brazilian Household Contacts
author Almeida, Elza Carmen de
author_facet Almeida, Elza Carmen de
Martinez, Alejandra Nóbrega
Maniero, Viviane Câmara
Sales, Ana Maria
Duppre, Nádia C.
Sarno, Euzenir Nunes
Santos, Adalberto Rezende
Moraes, Milton Ozório
author_role author
author2 Martinez, Alejandra Nóbrega
Maniero, Viviane Câmara
Sales, Ana Maria
Duppre, Nádia C.
Sarno, Euzenir Nunes
Santos, Adalberto Rezende
Moraes, Milton Ozório
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Almeida, Elza Carmen de
Martinez, Alejandra Nóbrega
Maniero, Viviane Câmara
Sales, Ana Maria
Duppre, Nádia C.
Sarno, Euzenir Nunes
Santos, Adalberto Rezende
Moraes, Milton Ozório
dc.subject.other.pt_BR.fl_str_mv Mycobacterium leprae
Reação em cadeia da polimerase
Contatos saudáveis
Diagnóstico precoce
topic Mycobacterium leprae
Reação em cadeia da polimerase
Contatos saudáveis
Diagnóstico precoce
Mycobacterium leprae
Polymerase chain reaction
Healthy contacts
Early diagnosis
dc.subject.en.pt_BR.fl_str_mv Mycobacterium leprae
Polymerase chain reaction
Healthy contacts
Early diagnosis
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Medicina Tropical. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.
publishDate 2004
dc.date.issued.fl_str_mv 2004
dc.date.accessioned.fl_str_mv 2019-05-30T11:43:58Z
dc.date.available.fl_str_mv 2019-05-30T11:43:58Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv ALMEIDA, Elza Carmen de et al. Detection of Mycobacterium leprae DNA by Polymerase Chain Reaction in the Blood and Nasal Secretion of Brazilian Household Contacts. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 99, n. 5, p. 509-512, Aug. 2004.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/33278
dc.identifier.issn.pt_BR.fl_str_mv 0074-0276
dc.identifier.doi.pt_BR.fl_str_mv 10.1590/S0074-02762004000500009
dc.identifier.eissn.none.fl_str_mv 1678-8060
identifier_str_mv ALMEIDA, Elza Carmen de et al. Detection of Mycobacterium leprae DNA by Polymerase Chain Reaction in the Blood and Nasal Secretion of Brazilian Household Contacts. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 99, n. 5, p. 509-512, Aug. 2004.
0074-0276
10.1590/S0074-02762004000500009
1678-8060
url https://www.arca.fiocruz.br/handle/icict/33278
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz
publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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