Replication origin location might contribute to genetic variability in Trypanosoma cruzi

Detalhes bibliográficos
Autor(a) principal: Araujo, Christiane Bezerra de
Data de Publicação: 2020
Outros Autores: Cunha, Julia Pinheiro Chagas da, Inada, Davi Toshio, Damasceno, Jeziel, Lima, Alex Ranieri Jerônimo, Hiraiwa, Priscila, Marques, Catarina, Gonçalves, Evonnildo, Nishiyama-Junior, Milton Yutaka, McCulloch, Richard, Elias, Maria Carolina
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/42493
Resumo: Instituto Butantan. Laboratório de Ciclo Celular. Butantã, SP, Brasil / Instituto Butantan. Centro de Toxinas, Resposta-imune e Sinalização Celular. Butantã, SP, Brasil.
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spelling Araujo, Christiane Bezerra deCunha, Julia Pinheiro Chagas daInada, Davi ToshioDamasceno, JezielLima, Alex Ranieri JerônimoHiraiwa, PriscilaMarques, CatarinaGonçalves, EvonnildoNishiyama-Junior, Milton YutakaMcCulloch, RichardElias, Maria Carolina2020-07-31T19:07:47Z2020-07-31T19:07:47Z2020ARAUJO, Christiane Bezerra de et al. Replication origin location might contribute to genetic variability in Trypanosoma cruzi. BMC Genomics, v. 21, n. 414, p. 1-16, 2020.1471-2164https://www.arca.fiocruz.br/handle/icict/4249310.1186/s12864-020-06803-81471-2164porBMCTrypanosoma cruziReplication OriginGenetic VariationInterspersed Repetitive SequencesOrigen de RéplicaVariación GenéticaSecuencias Repetitivas EsparcidasOrigem de ReplicaçãoVariação GenéticaSequências Repetitivas DispersasReplication origin location might contribute to genetic variability in Trypanosoma cruziinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleInstituto Butantan. Laboratório de Ciclo Celular. Butantã, SP, Brasil / Instituto Butantan. Centro de Toxinas, Resposta-imune e Sinalização Celular. Butantã, SP, Brasil.Instituto Butantan. Laboratório de Ciclo Celular. Butantã, SP, Brasil / Instituto Butantan. Centro de Toxinas, Resposta-imune e Sinalização Celular. Butantã, SP, Brasil.Instituto Butantan. Laboratório de Ciclo Celular. Butantã, SP, Brasil / Instituto Butantan. Centro de Toxinas, Resposta-imune e Sinalização Celular. Butantã, SP, Brasil.The Wellcome Centre for Molecular Parasitology. Institute of Infection, Immunity and Inflammation. University of Glasgow. Glasgow, UK.Universidade Federal do Pará. Instituto de Ciências Biológicas. Laboratório de Tecnologia Biomolecular – Bioinformática. Belém, PA, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.The Wellcome Centre for Molecular Parasitology. Institute of Infection, Immunity and Inflammation. University of Glasgow. Glasgow, UK.Universidade Federal do Pará. Instituto de Ciências Biológicas. Laboratório de Tecnologia Biomolecular – Bioinformática. Belém, PA, Brasil.Instituto Butantan. Centro de Toxinas, Resposta-imune e Sinalização Celular. Butantã, SP, Brasil / Instituto Butantan. Laboratório Especial de Toxinologia Aplicada. Butantã, SP, Brasil.The Wellcome Centre for Molecular Parasitology. Institute of Infection, Immunity and Inflammation. University of Glasgow. Glasgow, UK.Instituto Butantan. Laboratório de Ciclo Celular. Butantã, SP, Brasil / Instituto Butantan. Centro de Toxinas, Resposta-imune e Sinalização Celular. Butantã, SP, Brasil.DNA replication in trypanosomatids operates in a uniquely challenging environment, since most of their genomes are constitutively transcribed. Trypanosoma cruzi, the etiological agent of Chagas disease, presents high variability in both chromosomes size and copy number among strains, though the underlying mechanisms are unknown. As results had, we have mapped sites of DNA replication initiation across the T. cruzi genome using Marker Frequency Analysis, which has previously only been deployed in two related trypanosomatids. The putative origins identified in T.cruzi show a notable enrichment of GC content, a preferential position at subtelomeric regions, coinciding with genes transcribed towards the telomeres, and a pronounced enrichment within coding DNA sequences, most notably in genes from the Dispersed Gene Family 1 (DGF-1). Therefore as conclusions, these findings suggest a scenario where collisions between DNA replication and transcription are frequent, leading to increased genetic variability, as seen by the increase SNP levels at chromosome subtelomeres and in DGF-1 genes containing putative origins.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83084https://www.arca.fiocruz.br/bitstream/icict/42493/1/license.txt783568c2893d2e25a99990b126be1772MD51ORIGINALs12864-020-06803ok.pdfs12864-020-06803ok.pdfapplication/pdf3141909https://www.arca.fiocruz.br/bitstream/icict/42493/2/s12864-020-06803ok.pdf680574426203e8632df3f1817b47f74dMD52TEXTs12864-020-06803ok.pdf.txts12864-020-06803ok.pdf.txtExtracted texttext/plain70568https://www.arca.fiocruz.br/bitstream/icict/42493/3/s12864-020-06803ok.pdf.txt90f5478d0da45656e08f68c10e49c1d4MD53icict/424932020-09-06 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dc.title.pt_BR.fl_str_mv Replication origin location might contribute to genetic variability in Trypanosoma cruzi
title Replication origin location might contribute to genetic variability in Trypanosoma cruzi
spellingShingle Replication origin location might contribute to genetic variability in Trypanosoma cruzi
Araujo, Christiane Bezerra de
Trypanosoma cruzi
Replication Origin
Genetic Variation
Interspersed Repetitive Sequences
Origen de Réplica
Variación Genética
Secuencias Repetitivas Esparcidas
Origem de Replicação
Variação Genética
Sequências Repetitivas Dispersas
title_short Replication origin location might contribute to genetic variability in Trypanosoma cruzi
title_full Replication origin location might contribute to genetic variability in Trypanosoma cruzi
title_fullStr Replication origin location might contribute to genetic variability in Trypanosoma cruzi
title_full_unstemmed Replication origin location might contribute to genetic variability in Trypanosoma cruzi
title_sort Replication origin location might contribute to genetic variability in Trypanosoma cruzi
author Araujo, Christiane Bezerra de
author_facet Araujo, Christiane Bezerra de
Cunha, Julia Pinheiro Chagas da
Inada, Davi Toshio
Damasceno, Jeziel
Lima, Alex Ranieri Jerônimo
Hiraiwa, Priscila
Marques, Catarina
Gonçalves, Evonnildo
Nishiyama-Junior, Milton Yutaka
McCulloch, Richard
Elias, Maria Carolina
author_role author
author2 Cunha, Julia Pinheiro Chagas da
Inada, Davi Toshio
Damasceno, Jeziel
Lima, Alex Ranieri Jerônimo
Hiraiwa, Priscila
Marques, Catarina
Gonçalves, Evonnildo
Nishiyama-Junior, Milton Yutaka
McCulloch, Richard
Elias, Maria Carolina
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Araujo, Christiane Bezerra de
Cunha, Julia Pinheiro Chagas da
Inada, Davi Toshio
Damasceno, Jeziel
Lima, Alex Ranieri Jerônimo
Hiraiwa, Priscila
Marques, Catarina
Gonçalves, Evonnildo
Nishiyama-Junior, Milton Yutaka
McCulloch, Richard
Elias, Maria Carolina
dc.subject.other.pt_BR.fl_str_mv Trypanosoma cruzi
topic Trypanosoma cruzi
Replication Origin
Genetic Variation
Interspersed Repetitive Sequences
Origen de Réplica
Variación Genética
Secuencias Repetitivas Esparcidas
Origem de Replicação
Variação Genética
Sequências Repetitivas Dispersas
dc.subject.en.pt_BR.fl_str_mv Replication Origin
Genetic Variation
Interspersed Repetitive Sequences
dc.subject.es.pt_BR.fl_str_mv Origen de Réplica
Variación Genética
Secuencias Repetitivas Esparcidas
dc.subject.decs.pt_BR.fl_str_mv Origem de Replicação
Variação Genética
Sequências Repetitivas Dispersas
description Instituto Butantan. Laboratório de Ciclo Celular. Butantã, SP, Brasil / Instituto Butantan. Centro de Toxinas, Resposta-imune e Sinalização Celular. Butantã, SP, Brasil.
publishDate 2020
dc.date.accessioned.fl_str_mv 2020-07-31T19:07:47Z
dc.date.available.fl_str_mv 2020-07-31T19:07:47Z
dc.date.issued.fl_str_mv 2020
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv ARAUJO, Christiane Bezerra de et al. Replication origin location might contribute to genetic variability in Trypanosoma cruzi. BMC Genomics, v. 21, n. 414, p. 1-16, 2020.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/42493
dc.identifier.issn.pt_BR.fl_str_mv 1471-2164
dc.identifier.doi.none.fl_str_mv 10.1186/s12864-020-06803-8
dc.identifier.eissn.none.fl_str_mv 1471-2164
identifier_str_mv ARAUJO, Christiane Bezerra de et al. Replication origin location might contribute to genetic variability in Trypanosoma cruzi. BMC Genomics, v. 21, n. 414, p. 1-16, 2020.
1471-2164
10.1186/s12864-020-06803-8
url https://www.arca.fiocruz.br/handle/icict/42493
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dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv BMC
publisher.none.fl_str_mv BMC
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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instacron:FIOCRUZ
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institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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