The Usefulness of a Duplex RT-qPCR during the Recent Yellow Fever Brazilian Epidemic: Surveillance of Vaccine Adverse Events, Epizootics and Vectors
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2021 |
| Outros Autores: | , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/48486 |
Resumo: | Instituto Evandro Chagas. Departamento de Arbovirologia e Febres Hemorrágicas. Ananindeua, PA, Brasil. |
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Queiroz, Alice L. N.Barros, Rafael S.Silva, Sandro P.Rodrigues, Daniela S. G.Cruz, Ana C. R.Santos, Flávia B. dosVasconcelos, Pedro F. C.Tesh, Roberto B.Nunes, Bruno T. D.Medeiros, Daniele B. A.2021-08-03T14:06:48Z2021-08-03T14:06:48Z2021QUEIROZ, Alice L. N. et al. The Usefulness of a Duplex RT-qPCR during the Recent Yellow Fever Brazilian Epidemic: Surveillance of Vaccine Adverse Events, Epizootics and Vectors. Pathogens, v. 10, 693, 16 p, June 2021.2076-0817https://www.arca.fiocruz.br/handle/icict/4848610.3390/pathogens10060693engMDPIFebre AmarelaEfeitos adversos da vacinaDuplex RT-qPCRBrasilYellow feverDuplex RT-qPCRVaccine adverse effectsBrazilThe Usefulness of a Duplex RT-qPCR during the Recent Yellow Fever Brazilian Epidemic: Surveillance of Vaccine Adverse Events, Epizootics and Vectorsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleInstituto Evandro Chagas. Departamento de Arbovirologia e Febres Hemorrágicas. Ananindeua, PA, Brasil.Instituto Evandro Chagas. Departamento de Arbovirologia e Febres Hemorrágicas. Ananindeua, PA, Brasil.Instituto Evandro Chagas. Departamento de Arbovirologia e Febres Hemorrágicas. Ananindeua, PA, Brasil.Instituto Evandro Chagas. Departamento de Arbovirologia e Febres Hemorrágicas. Ananindeua, PA, Brasil.Instituto Evandro Chagas. Departamento de Arbovirologia e Febres Hemorrágicas. Ananindeua, PA, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ, Brasil.Instituto Evandro Chagas. Departamento de Arbovirologia e Febres Hemorrágicas. Ananindeua, PA, Brasil.University Texas Medical Branch. Department of Pathology and Microbiology & Immunology. Galveston, TX, USA.Instituto Evandro Chagas. Departamento de Arbovirologia e Febres Hemorrágicas. Ananindeua, PA, Brasil.Instituto Evandro Chagas. Departamento de Arbovirologia e Febres Hemorrágicas. Ananindeua, PA, Brasil.From 2016 to 2018, Brazil faced the biggest yellow fever (YF) outbreak in the last 80 years, representing a risk of YF reurbanization, especially in megacities. Along with this challenge, the mass administration of the fractionated YF vaccine dose in a naïve population brought another concern: the possibility to increase YF adverse events associated with viscerotropic (YEL-AVD) or neurological disease (YEL-AND). For this reason, we developed a quantitative real time RT-PCR (RT-qPCR) assay based on a duplex TaqMan protocol to distinguish broad-spectrum infections caused by wild-type yellow fever virus (YFV) strain from adverse events following immunization (AEFI) by 17DD strain during the vaccination campaign used to contain this outbreak. A rapid and more accurate RT-qPCR assay to diagnose YFV was established, being able to detect even different YFV genotypes and geographic strains that circulate in Central and South America. Moreover, after testing around 1400 samples from human cases, non-human primates and mosquitoes, we detected just two YEL-AVD cases, confirmed by sequencing, during the massive vaccination in Brazilian Southeast region, showing lower incidence than AEFI as expected.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/48486/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALFlaviaBdos_Santos_etal_IOC_2021.pdfFlaviaBdos_Santos_etal_IOC_2021.pdfapplication/pdf2027273https://www.arca.fiocruz.br/bitstream/icict/48486/2/FlaviaBdos_Santos_etal_IOC_2021.pdf60e1c61417e2566dbed420c82861a40cMD52TEXTFlaviaBdos_Santos_etal_IOC_2021.pdf.txtFlaviaBdos_Santos_etal_IOC_2021.pdf.txtExtracted 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| dc.title.pt_BR.fl_str_mv |
The Usefulness of a Duplex RT-qPCR during the Recent Yellow Fever Brazilian Epidemic: Surveillance of Vaccine Adverse Events, Epizootics and Vectors |
| title |
The Usefulness of a Duplex RT-qPCR during the Recent Yellow Fever Brazilian Epidemic: Surveillance of Vaccine Adverse Events, Epizootics and Vectors |
| spellingShingle |
The Usefulness of a Duplex RT-qPCR during the Recent Yellow Fever Brazilian Epidemic: Surveillance of Vaccine Adverse Events, Epizootics and Vectors Queiroz, Alice L. N. Febre Amarela Efeitos adversos da vacina Duplex RT-qPCR Brasil Yellow fever Duplex RT-qPCR Vaccine adverse effects Brazil |
| title_short |
The Usefulness of a Duplex RT-qPCR during the Recent Yellow Fever Brazilian Epidemic: Surveillance of Vaccine Adverse Events, Epizootics and Vectors |
| title_full |
The Usefulness of a Duplex RT-qPCR during the Recent Yellow Fever Brazilian Epidemic: Surveillance of Vaccine Adverse Events, Epizootics and Vectors |
| title_fullStr |
The Usefulness of a Duplex RT-qPCR during the Recent Yellow Fever Brazilian Epidemic: Surveillance of Vaccine Adverse Events, Epizootics and Vectors |
| title_full_unstemmed |
The Usefulness of a Duplex RT-qPCR during the Recent Yellow Fever Brazilian Epidemic: Surveillance of Vaccine Adverse Events, Epizootics and Vectors |
| title_sort |
The Usefulness of a Duplex RT-qPCR during the Recent Yellow Fever Brazilian Epidemic: Surveillance of Vaccine Adverse Events, Epizootics and Vectors |
| author |
Queiroz, Alice L. N. |
| author_facet |
Queiroz, Alice L. N. Barros, Rafael S. Silva, Sandro P. Rodrigues, Daniela S. G. Cruz, Ana C. R. Santos, Flávia B. dos Vasconcelos, Pedro F. C. Tesh, Roberto B. Nunes, Bruno T. D. Medeiros, Daniele B. A. |
| author_role |
author |
| author2 |
Barros, Rafael S. Silva, Sandro P. Rodrigues, Daniela S. G. Cruz, Ana C. R. Santos, Flávia B. dos Vasconcelos, Pedro F. C. Tesh, Roberto B. Nunes, Bruno T. D. Medeiros, Daniele B. A. |
| author2_role |
author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Queiroz, Alice L. N. Barros, Rafael S. Silva, Sandro P. Rodrigues, Daniela S. G. Cruz, Ana C. R. Santos, Flávia B. dos Vasconcelos, Pedro F. C. Tesh, Roberto B. Nunes, Bruno T. D. Medeiros, Daniele B. A. |
| dc.subject.other.pt_BR.fl_str_mv |
Febre Amarela Efeitos adversos da vacina Duplex RT-qPCR Brasil |
| topic |
Febre Amarela Efeitos adversos da vacina Duplex RT-qPCR Brasil Yellow fever Duplex RT-qPCR Vaccine adverse effects Brazil |
| dc.subject.en.pt_BR.fl_str_mv |
Yellow fever Duplex RT-qPCR Vaccine adverse effects Brazil |
| description |
Instituto Evandro Chagas. Departamento de Arbovirologia e Febres Hemorrágicas. Ananindeua, PA, Brasil. |
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2021 |
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2021-08-03T14:06:48Z |
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2021-08-03T14:06:48Z |
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2021 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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QUEIROZ, Alice L. N. et al. The Usefulness of a Duplex RT-qPCR during the Recent Yellow Fever Brazilian Epidemic: Surveillance of Vaccine Adverse Events, Epizootics and Vectors. Pathogens, v. 10, 693, 16 p, June 2021. |
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https://www.arca.fiocruz.br/handle/icict/48486 |
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2076-0817 |
| dc.identifier.doi.none.fl_str_mv |
10.3390/pathogens10060693 |
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QUEIROZ, Alice L. N. et al. The Usefulness of a Duplex RT-qPCR during the Recent Yellow Fever Brazilian Epidemic: Surveillance of Vaccine Adverse Events, Epizootics and Vectors. Pathogens, v. 10, 693, 16 p, June 2021. 2076-0817 10.3390/pathogens10060693 |
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