A novel flagellar sheath protein, FcpA, determines filament coiling, translational motility and virulence for the Leptospira spirochete
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2016 |
| Outros Autores: | , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/15272 |
Resumo: | National Institutes of Health. Brazilian National Research Council (CNPq). Welch Foundation. |
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Wunder Junior, Elsio AugustoFigueira, Cláudio PereiraBenaroudj, NadiaHu, BoTong, Brian ATrajtenberg, FelipeLiu, JunReis, Mitermayer Galvão dosCharon, Nyles WBuschiazzo, AlejandroPicardeau, MathieuKo, Albert Icksang2016-08-16T16:39:34Z2016-08-16T16:39:34Z2016WUNDER JÚNIOR, E. A. et al. A novel flagellar sheath protein, FcpA, determines filament coiling, translational motility and virulence for the Leptospira spirochete. Molecular Microbiology, 2016.0950-382Xhttps://www.arca.fiocruz.br/handle/icict/1527210.1111/mmi.13403National Institutes of Health. Brazilian National Research Council (CNPq). Welch Foundation.Yale School of Public Health. Department of Epidemiology of Microbial Disease. New Haven, Connecticut, USA / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilInstitut Pasteur. Unit of Biology of Spirochetes. Paris, FranceUniversity of Texas Medical School at Houston. Department of Pathology and Laboratory Medicine. Houston, USAUniversity of Texas Medical School at Houston. Department of Pathology and Laboratory Medicine. Houston, USAInstitut Pasteur de Montevideo. Laboratory of Molecular & Structural Microbiology. Montevideo, UruguayUniversity of Texas Medical School at Houston. Department of Pathology and Laboratory Medicine. Houston, USAFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilWest Virginia University. Department of Microbiology and ImmunologyMorgantown. WV, USAUniversity of Texas Medical School at Houston. Department of Pathology and Laboratory Medicine. Houston, USA / Institute Pasteur. Department of Structural Biology and Chemistry. Paris, France.Institut Pasteur. Unit of Biology of Spirochetes. Paris, FranceYale School of Public Health. Department of Epidemiology of Microbial Disease. New Haven, Connecticut, USA / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilLeptospira are unique among bacteria based on their helical cell morphology with hook-shaped ends and the presence of periplasmic flagella (PF) with pronounced spontaneous supercoiling. The factors that provoke such supercoiling, as well as the role that PF coiling plays in generating the characteristic hook-end cell morphology and motility, have not been elucidated. We have now identified an abundant protein from the pathogen L. interrogans, exposed on the PF surface, and named it Flagellar-coiling protein A (FcpA). The gene encoding FcpA is highly conserved among Leptospira and was not found in other bacteria. fcpA(-) mutants, obtained from clinical isolates or by allelic exchange, had relatively straight, smaller-diameter PF, and were not able to produce translational motility. These mutants lost their ability to cause disease in the standard hamster model of leptospirosis. Complementation of fcpA restored the wild-type morphology, motility and virulence phenotypes. In summary, we identified a novel Leptospira 36-kDa protein, the main component of the spirochete's PF sheath, and a key determinant of the flagella's coiled structure. FcpA is essential for bacterial translational motility and to enable the spirochete to penetrate the host, traverse tissue barriers, disseminate to cause systemic infection and reach target organs.engWileyA novel flagellar sheath protein, FcpA, determines filament coiling, translational motility and virulence for the Leptospira spirocheteinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleLeptospiraLeptospirosisSpirocheteVirulenceFlagellaPhenotypesAnimalsCricetinaeinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/15272/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALWunder Jr EA A novel flagellar....pdfWunder Jr EA A novel 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| dc.title.pt_BR.fl_str_mv |
A novel flagellar sheath protein, FcpA, determines filament coiling, translational motility and virulence for the Leptospira spirochete |
| title |
A novel flagellar sheath protein, FcpA, determines filament coiling, translational motility and virulence for the Leptospira spirochete |
| spellingShingle |
A novel flagellar sheath protein, FcpA, determines filament coiling, translational motility and virulence for the Leptospira spirochete Wunder Junior, Elsio Augusto Leptospira Leptospirosis Spirochete Virulence Flagella Phenotypes Animals Cricetinae |
| title_short |
A novel flagellar sheath protein, FcpA, determines filament coiling, translational motility and virulence for the Leptospira spirochete |
| title_full |
A novel flagellar sheath protein, FcpA, determines filament coiling, translational motility and virulence for the Leptospira spirochete |
| title_fullStr |
A novel flagellar sheath protein, FcpA, determines filament coiling, translational motility and virulence for the Leptospira spirochete |
| title_full_unstemmed |
A novel flagellar sheath protein, FcpA, determines filament coiling, translational motility and virulence for the Leptospira spirochete |
| title_sort |
A novel flagellar sheath protein, FcpA, determines filament coiling, translational motility and virulence for the Leptospira spirochete |
| author |
Wunder Junior, Elsio Augusto |
| author_facet |
Wunder Junior, Elsio Augusto Figueira, Cláudio Pereira Benaroudj, Nadia Hu, Bo Tong, Brian A Trajtenberg, Felipe Liu, Jun Reis, Mitermayer Galvão dos Charon, Nyles W Buschiazzo, Alejandro Picardeau, Mathieu Ko, Albert Icksang |
| author_role |
author |
| author2 |
Figueira, Cláudio Pereira Benaroudj, Nadia Hu, Bo Tong, Brian A Trajtenberg, Felipe Liu, Jun Reis, Mitermayer Galvão dos Charon, Nyles W Buschiazzo, Alejandro Picardeau, Mathieu Ko, Albert Icksang |
| author2_role |
author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Wunder Junior, Elsio Augusto Figueira, Cláudio Pereira Benaroudj, Nadia Hu, Bo Tong, Brian A Trajtenberg, Felipe Liu, Jun Reis, Mitermayer Galvão dos Charon, Nyles W Buschiazzo, Alejandro Picardeau, Mathieu Ko, Albert Icksang |
| dc.subject.en.pt_BR.fl_str_mv |
Leptospira Leptospirosis Spirochete Virulence Flagella Phenotypes Animals Cricetinae |
| topic |
Leptospira Leptospirosis Spirochete Virulence Flagella Phenotypes Animals Cricetinae |
| description |
National Institutes of Health. Brazilian National Research Council (CNPq). Welch Foundation. |
| publishDate |
2016 |
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2016-08-16T16:39:34Z |
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2016-08-16T16:39:34Z |
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2016 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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WUNDER JÚNIOR, E. A. et al. A novel flagellar sheath protein, FcpA, determines filament coiling, translational motility and virulence for the Leptospira spirochete. Molecular Microbiology, 2016. |
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https://www.arca.fiocruz.br/handle/icict/15272 |
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0950-382X |
| dc.identifier.doi.none.fl_str_mv |
10.1111/mmi.13403 |
| identifier_str_mv |
WUNDER JÚNIOR, E. A. et al. A novel flagellar sheath protein, FcpA, determines filament coiling, translational motility and virulence for the Leptospira spirochete. Molecular Microbiology, 2016. 0950-382X 10.1111/mmi.13403 |
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Wiley |
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Wiley |
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