Molecular and phenotypic characteristics of methicillin-resistant Staphylococcus aureus isolated from hospitalized patients
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2015 |
| Outros Autores: | , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | por |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/15544 |
Resumo: | This study was supported by grants from Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)–PROAP, Programa de Pós-Graduação em Microbiologia da Universidade Estadual de Londrina. |
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Oliveira, Caio Ferreira deMorey, Alexandre TadachiSantos, Jussevania PereiraGomes, Ludmila Vilelela PereiraCardoso, Jucélio DonizetePinge-Filho, PhilenoPerugini, Marcia Regina EnchesYamauchi, Lucy MegumiOgatta, Sueli Fumie Yamada2016-08-30T16:32:24Z2016-08-30T16:32:24Z2015OLIVEIRA, Caio F. de et al. Molecular and phenotypic characteristics of methicillin-resistant Staphylococcus aureus isolated from hospitalized patients Journal of Infection in Developing Countries, v. 9, n. 7, p. 743-751, 2015.1972-2680https://www.arca.fiocruz.br/handle/icict/1554410.3855/jidc.5868This study was supported by grants from Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)–PROAP, Programa de Pós-Graduação em Microbiologia da Universidade Estadual de Londrina.Universidade Estadual de Londrina. Centro de Ciências Biológicas. Departamento de Microbiologia. Laboratório de Biologia Molecular de Microrganismos. Londrina, PR, Brasil.Universidade Estadual de Londrina. Centro de Ciências Biológicas. Departamento de Microbiologia. Laboratório de Biologia Molecular de Microrganismos. Londrina, PR, Brasil.Universidade Estadual de Londrina. Centro de Ciências Biológicas. Departamento de Microbiologia. Laboratório de Biologia Molecular de Microrganismos. Londrina, PR, Brasil.Universidade Estadual de Londrina. Centro de Ciências Biológicas. Departamento de Microbiologia. Laboratório de Biologia Molecular de Microrganismos. Londrina, PR, Brasil.Instituto Agronômico do Paraná. Departamento de Microbiologia do Solo. Laboratório de Microbiologia do Solo. Londrina, PR, Brasil.Universidade Estadual de Londrina. Centro de Ciências Biológicas. Departamento de Ciências Patológicas. Laboratório de Imunologia. Londrina, PR, Brasil / Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Universidade Estadual de Londrina. Centro de Ciências da Saúde. Departamento de Patologia. Análises Clínicas e Toxicológicas. Londrina, PR, Brasil.Universidade Estadual de Londrina. Centro de Ciências Biológicas. Departamento de Microbiologia. Laboratório de Biologia Molecular de Microrganismos. Londrina, PR, Brasil.Universidade Estadual de Londrina. Centro de Ciências Biológicas. Departamento de Microbiologia. Laboratório de Biologia Molecular de Microrganismos. Londrina, PR, Brasil.Introduction: Methicillin-resistant Staphylococcus aureus (MRSA) is one of the leading causes of infections acquired in both community and hospital settings. In this study, MRSA isolated from different sources of hospitalized patients was characterized by molecular and phenotypic methods. Methodology: A total of 123 S. aureus isolates were characterized according to their genetic relatedness by repetitive element sequence based-PCR (REP-PCR), in vitro antimicrobial susceptibility profile, SCCmec typing and presence of seven virulence factor-encoding genes. Results: REP-PCR fingerprinting showed low relatedness between the isolates, and the predominance of one specific lineage or clonal group was not observed. All isolates were susceptible to teicoplanin and linezolide. All isolates were resistant to cefoxitin and penicillin, and the majority were also resistant to one or more other antimicrobials. Fifty isolates (41.7%) were intermediately resistant to vancomycin. Most isolates harbored SCCmec type II (53.7%), followed by type I (22.8%), type IV (8.1%) and type III (1.6%). All isolates harbored at least two virulence factor-encoding genes, and the prevalence was as follows: coa, 100%; icaA, 100%; hla, 13.0%; hlb, 91.1%, hld, 91.1%; lukS-PV and lukF-PV, 2.4%; and tst, 34.1%. A positive association with the presence of hla and SCCmec type II, and tst and SCCmec type I was observed. Conclusion: This study showed the high virulence potential of multidrug-resistant MRSA circulating in a teaching hospital. A high prevalence of MRSA showing intermediate vancomycin resistance was also observed, indicating the urgent need to improve strategies for controlling the use of antimicrobials for appropriate management of S. aureus infections.porMolecular and phenotypic characteristics of methicillin-resistant Staphylococcus aureus isolated from hospitalized patientsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleAntimicrobial resistanceIntermediate vancomycin resistanceMRSASCCmec typingVirulence factorsinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/15544/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALMolecular and phenotypic characteristics of methicillin-resistant Staphylococcus aureus isolated from hospitalized patients.pdfMolecular and phenotypic characteristics of 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| dc.title.pt_BR.fl_str_mv |
Molecular and phenotypic characteristics of methicillin-resistant Staphylococcus aureus isolated from hospitalized patients |
| title |
Molecular and phenotypic characteristics of methicillin-resistant Staphylococcus aureus isolated from hospitalized patients |
| spellingShingle |
Molecular and phenotypic characteristics of methicillin-resistant Staphylococcus aureus isolated from hospitalized patients Oliveira, Caio Ferreira de Antimicrobial resistance Intermediate vancomycin resistance MRSA SCCmec typing Virulence factors |
| title_short |
Molecular and phenotypic characteristics of methicillin-resistant Staphylococcus aureus isolated from hospitalized patients |
| title_full |
Molecular and phenotypic characteristics of methicillin-resistant Staphylococcus aureus isolated from hospitalized patients |
| title_fullStr |
Molecular and phenotypic characteristics of methicillin-resistant Staphylococcus aureus isolated from hospitalized patients |
| title_full_unstemmed |
Molecular and phenotypic characteristics of methicillin-resistant Staphylococcus aureus isolated from hospitalized patients |
| title_sort |
Molecular and phenotypic characteristics of methicillin-resistant Staphylococcus aureus isolated from hospitalized patients |
| author |
Oliveira, Caio Ferreira de |
| author_facet |
Oliveira, Caio Ferreira de Morey, Alexandre Tadachi Santos, Jussevania Pereira Gomes, Ludmila Vilelela Pereira Cardoso, Jucélio Donizete Pinge-Filho, Phileno Perugini, Marcia Regina Enches Yamauchi, Lucy Megumi Ogatta, Sueli Fumie Yamada |
| author_role |
author |
| author2 |
Morey, Alexandre Tadachi Santos, Jussevania Pereira Gomes, Ludmila Vilelela Pereira Cardoso, Jucélio Donizete Pinge-Filho, Phileno Perugini, Marcia Regina Enches Yamauchi, Lucy Megumi Ogatta, Sueli Fumie Yamada |
| author2_role |
author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Oliveira, Caio Ferreira de Morey, Alexandre Tadachi Santos, Jussevania Pereira Gomes, Ludmila Vilelela Pereira Cardoso, Jucélio Donizete Pinge-Filho, Phileno Perugini, Marcia Regina Enches Yamauchi, Lucy Megumi Ogatta, Sueli Fumie Yamada |
| dc.subject.en.pt_BR.fl_str_mv |
Antimicrobial resistance Intermediate vancomycin resistance MRSA SCCmec typing Virulence factors |
| topic |
Antimicrobial resistance Intermediate vancomycin resistance MRSA SCCmec typing Virulence factors |
| description |
This study was supported by grants from Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)–PROAP, Programa de Pós-Graduação em Microbiologia da Universidade Estadual de Londrina. |
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2015 |
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2015 |
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2016-08-30T16:32:24Z |
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2016-08-30T16:32:24Z |
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info:eu-repo/semantics/article |
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OLIVEIRA, Caio F. de et al. Molecular and phenotypic characteristics of methicillin-resistant Staphylococcus aureus isolated from hospitalized patients Journal of Infection in Developing Countries, v. 9, n. 7, p. 743-751, 2015. |
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https://www.arca.fiocruz.br/handle/icict/15544 |
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1972-2680 |
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10.3855/jidc.5868 |
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OLIVEIRA, Caio F. de et al. Molecular and phenotypic characteristics of methicillin-resistant Staphylococcus aureus isolated from hospitalized patients Journal of Infection in Developing Countries, v. 9, n. 7, p. 743-751, 2015. 1972-2680 10.3855/jidc.5868 |
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