Replacement of Animals Used in the Maintenance of Aedes Aegypti by Alternative Methods. Refinement of Procedures That Minimize the Discomfort of Insect Maintenance by Swiss Webster Mice

Detalhes bibliográficos
Autor(a) principal: Oliveira, Gabriel
Data de Publicação: 2022
Outros Autores: Maria Julia, Rute
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/51669
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil.
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spelling Oliveira, GabrielMaria Julia, Rute2022-03-09T21:30:10Z2022-03-09T21:30:10Z2022OLIVEIRA, Gabriel; JULIA, Rute Maria. Replacement of Animals Used in the Maintenance of Aedes Aegypti by Alternative Methods. Refinement of Procedures That Minimize the Discomfort of Insect Maintenance by Swiss Webster Mice. Open Access Journal of Biomedical Science, v. 4, n. 2, p. 1631-1634, 2022.2690-487Xhttps://www.arca.fiocruz.br/handle/icict/5166910.38125/OAJBS.000409engScience Publishing GroupAnimalRefeição de sangueSubstituirRefinar o sistemaAnimalBlood mealReplaceRefine systemReplacement of Animals Used in the Maintenance of Aedes Aegypti by Alternative Methods. Refinement of Procedures That Minimize the Discomfort of Insect Maintenance by Swiss Webster Miceinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Doenças Parasitárias. Rio de Janeiro, RJ, Brasil.Meal) and breeding of hematophagous insects, vectors of serious and (often) neglected diseases. In summary, the main objectives of the project are: a) Replacement of the use of mice (Mus musculus) for the blood meal of Aedes aegypti in the laboratory. b) In case of failure to replace the animal, that is, in all phases of the life cycle of Aedes aegypti, we will test the application of two refinements in the procedure: The first is based on the use of Erythropoietin, increasing the number of and we hope to speed up the time to food satiety for the mosquito and the second to use an analgesic, an opiate with minimal adverse effects. Complementing, in the case of successful use of Erythropoietin. We will apply the association of the recombinant hormone with the analgesic for mice during the Aedes aegypti Blood Meal in the laboratory aiming, firstly, to avoid the use of the animal. However, if it is not possible to apply it in the maintenance routine of Aedes aegypti to minimize the stress and discomfort of this animal during feeding and creation of hematophagous vectors, in our case, the mosquito.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/51669/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALGabrielOliveira_RuthMariaJulia_IOC_2022.pdfGabrielOliveira_RuthMariaJulia_IOC_2022.pdfapplication/pdf366520https://www.arca.fiocruz.br/bitstream/icict/51669/2/GabrielOliveira_RuthMariaJulia_IOC_2022.pdf93912693eb37d69bbd42a1702375709bMD52icict/516692022-03-09 18:30:10.36oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352022-03-09T21:30:10Repositório Institucional da FIOCRUZ (ARCA) - 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dc.title.pt_BR.fl_str_mv Replacement of Animals Used in the Maintenance of Aedes Aegypti by Alternative Methods. Refinement of Procedures That Minimize the Discomfort of Insect Maintenance by Swiss Webster Mice
title Replacement of Animals Used in the Maintenance of Aedes Aegypti by Alternative Methods. Refinement of Procedures That Minimize the Discomfort of Insect Maintenance by Swiss Webster Mice
spellingShingle Replacement of Animals Used in the Maintenance of Aedes Aegypti by Alternative Methods. Refinement of Procedures That Minimize the Discomfort of Insect Maintenance by Swiss Webster Mice
Oliveira, Gabriel
Animal
Refeição de sangue
Substituir
Refinar o sistema
Animal
Blood meal
Replace
Refine system
title_short Replacement of Animals Used in the Maintenance of Aedes Aegypti by Alternative Methods. Refinement of Procedures That Minimize the Discomfort of Insect Maintenance by Swiss Webster Mice
title_full Replacement of Animals Used in the Maintenance of Aedes Aegypti by Alternative Methods. Refinement of Procedures That Minimize the Discomfort of Insect Maintenance by Swiss Webster Mice
title_fullStr Replacement of Animals Used in the Maintenance of Aedes Aegypti by Alternative Methods. Refinement of Procedures That Minimize the Discomfort of Insect Maintenance by Swiss Webster Mice
title_full_unstemmed Replacement of Animals Used in the Maintenance of Aedes Aegypti by Alternative Methods. Refinement of Procedures That Minimize the Discomfort of Insect Maintenance by Swiss Webster Mice
title_sort Replacement of Animals Used in the Maintenance of Aedes Aegypti by Alternative Methods. Refinement of Procedures That Minimize the Discomfort of Insect Maintenance by Swiss Webster Mice
author Oliveira, Gabriel
author_facet Oliveira, Gabriel
Maria Julia, Rute
author_role author
author2 Maria Julia, Rute
author2_role author
dc.contributor.author.fl_str_mv Oliveira, Gabriel
Maria Julia, Rute
dc.subject.other.pt_BR.fl_str_mv Animal
Refeição de sangue
Substituir
Refinar o sistema
topic Animal
Refeição de sangue
Substituir
Refinar o sistema
Animal
Blood meal
Replace
Refine system
dc.subject.en.pt_BR.fl_str_mv Animal
Blood meal
Replace
Refine system
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil.
publishDate 2022
dc.date.accessioned.fl_str_mv 2022-03-09T21:30:10Z
dc.date.available.fl_str_mv 2022-03-09T21:30:10Z
dc.date.issued.fl_str_mv 2022
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv OLIVEIRA, Gabriel; JULIA, Rute Maria. Replacement of Animals Used in the Maintenance of Aedes Aegypti by Alternative Methods. Refinement of Procedures That Minimize the Discomfort of Insect Maintenance by Swiss Webster Mice. Open Access Journal of Biomedical Science, v. 4, n. 2, p. 1631-1634, 2022.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/51669
dc.identifier.issn.pt_BR.fl_str_mv 2690-487X
dc.identifier.doi.none.fl_str_mv 10.38125/OAJBS.000409
identifier_str_mv OLIVEIRA, Gabriel; JULIA, Rute Maria. Replacement of Animals Used in the Maintenance of Aedes Aegypti by Alternative Methods. Refinement of Procedures That Minimize the Discomfort of Insect Maintenance by Swiss Webster Mice. Open Access Journal of Biomedical Science, v. 4, n. 2, p. 1631-1634, 2022.
2690-487X
10.38125/OAJBS.000409
url https://www.arca.fiocruz.br/handle/icict/51669
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Science Publishing Group
publisher.none.fl_str_mv Science Publishing Group
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/51669/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/51669/2/GabrielOliveira_RuthMariaJulia_IOC_2022.pdf
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
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