Leishmania enriettii: biochemical characterisation of lipophosphoglycans (LPGs) and glycoinositolphospholipids (GIPLs) and infectivity to Cavia porcellus.

Detalhes bibliográficos
Autor(a) principal: Paranaíba, Larissa Ferreira
Data de Publicação: 2015
Outros Autores: Assis, Rafael Ramiro de, Nogueira, Paula Monalisa, Torrecilhas, Ana Claúdia, Campos, João Henrique, Silveira, Amanda Cardoso de Oliveira, Martins Filho, Olindo Assis, Pessoa, Natalia Lima, Campos, Marco Antônio, Parreiras, Patrícia Martins, Melo, Maria Norma, Gontijo, Nelder de Figueiredo, Soares, Rodrigo Pedro Pinto
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
DOI: 10.1186/s13071-015-0633-8
Texto Completo: https://www.arca.fiocruz.br/handle/icict/12606
Resumo: Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil/Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Laboratório de Fisiologia de Insetos Hematófagos. Belo Horizonte, MG, Brasil.
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spelling Paranaíba, Larissa FerreiraAssis, Rafael Ramiro deNogueira, Paula MonalisaTorrecilhas, Ana ClaúdiaCampos, João HenriqueSilveira, Amanda Cardoso de OliveiraMartins Filho, Olindo AssisPessoa, Natalia LimaCampos, Marco AntônioParreiras, Patrícia MartinsMelo, Maria NormaGontijo, Nelder de FigueiredoSoares, Rodrigo Pedro Pinto2016-01-25T17:10:46Z2016-01-25T17:10:46Z2015PARANAÍBA, Larissa Ferreira et al. Leishmania enriettii: biochemical characterisation of lipophosphoglycans (LPGs) and glycoinositolphospholipids (GIPLs) and infectivity to Cavia porcellus. Parasit Vectors., vol 8, p. 31, 20151756-3305https://www.arca.fiocruz.br/handle/icict/1260610.1186/s13071-015-0633-8engBioMed CentralLeishmania enriettii: biochemical characterisation of lipophosphoglycans (LPGs) and glycoinositolphospholipids (GIPLs) and infectivity to Cavia porcellus.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil/Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Laboratório de Fisiologia de Insetos Hematófagos. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratorio de Parasitologia Celular e Molecular. Belo Horizonte, MG, Brasil.Universidade Federal de São Paulo. Departamento de Ciências Biológicas. Laboratório de Imunologia Celular e Bioquímica de Fungos e Protozoários. São Paulo, SP, Brasil.Universidade Federal de São Paulo. Departamento de Ciências Biológicas. Laboratório de Imunologia Celular e Bioquímica de Fungos e Protozoários. São Paulo, SP, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Laboratório de Biologia de Leishmania. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Laboratório de Fisiologia de Insetos Hematófagos. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratorio de Parasitologia Celular e Molecular. Belo Horizonte, MG, Brasil.BACKGROUND: Leishmania enriettii is a species non-infectious to man, whose reservoir is the guinea pig Cavia porcellus. Many aspects of the parasite-host interaction in this model are unknown, especially those involving parasite surface molecules. While lipophosphoglycans (LPGs) and glycoinositolphospholipids (GIPLs) of Leishmania species from the Old and New World have already been described, glycoconjugates of L. enriettii and their importance are still unknown. METHODS: Mice peritoneal macrophages from C57BL/6 and knock-out (TLR2 -/-, TLR4 -/-) were primed with IFN-γ and stimulated with purified LPG and GIPLs from both species. Nitric oxide and cytokine production were performed. MAPKs (p38 and JNK) and NF-kB activation were evaluated in J774.1 macrophages and CHO cells, respectively. RESULTS: LPGs were extracted, purified and analysed by western-blot, showing that LPG from L88 strain was longer than that of Cobaia strain. LPGs and GIPLs were depolymerised and their sugar content was determined. LPGs from both strains did not present side chains, having the common disaccharide Gal(β1,4)Man(α1)-PO4. The GIPL from L88 strain presented galactose in its structure, suggestive of type II GIPL. On the other hand, the GIPL of Cobaia strain presented an abundance of glucose, a characteristic not previously observed. Mice peritoneal macrophages from C57BL/6 and knock-outs (TLR2 -/- and TLR4 -/-) were primed with IFN-γ and stimulated with glycoconjugates and live parasites. No activation of NO or cytokines was observed with live parasites. On the other hand, LPGs and GIPLs were able to activate the production of NO, IL-6, IL-12 and TNF-α preferably via TRL2. However, in CHO cells, only GIPLs were able to activate TRL2 and TRL4. In vivo studies using male guinea pigs (Cavia porcellus) showed that only strain L88 was able to develop more severe ulcerated lesions especially in the presence of salivary gland extract (SGE). CONCLUSION: The two L. enriettii strains exhibited polymorphisms in their LPGs and GIPLs and those features may be related to a more pro-inflammatory profile in the L88 strain.Leishmania enriettiiMicepolymorphismsinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/12606/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALLeishmania enriettii.pdfLeishmania enriettii.pdfapplication/pdf13520683https://www.arca.fiocruz.br/bitstream/icict/12606/2/Leishmania%20enriettii.pdfddf0e80aad9a64a340113e7ebc6d6fb2MD52TEXTLeishmania enriettii.pdf.txtLeishmania enriettii.pdf.txtExtracted texttext/plain729https://www.arca.fiocruz.br/bitstream/icict/12606/3/Leishmania%20enriettii.pdf.txt58961bb979173a2c29b8d2fccab4a0b4MD53icict/126062022-01-31 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dc.title.pt_BR.fl_str_mv Leishmania enriettii: biochemical characterisation of lipophosphoglycans (LPGs) and glycoinositolphospholipids (GIPLs) and infectivity to Cavia porcellus.
title Leishmania enriettii: biochemical characterisation of lipophosphoglycans (LPGs) and glycoinositolphospholipids (GIPLs) and infectivity to Cavia porcellus.
spellingShingle Leishmania enriettii: biochemical characterisation of lipophosphoglycans (LPGs) and glycoinositolphospholipids (GIPLs) and infectivity to Cavia porcellus.
Paranaíba, Larissa Ferreira
Leishmania enriettii
Mice
polymorphisms
title_short Leishmania enriettii: biochemical characterisation of lipophosphoglycans (LPGs) and glycoinositolphospholipids (GIPLs) and infectivity to Cavia porcellus.
title_full Leishmania enriettii: biochemical characterisation of lipophosphoglycans (LPGs) and glycoinositolphospholipids (GIPLs) and infectivity to Cavia porcellus.
title_fullStr Leishmania enriettii: biochemical characterisation of lipophosphoglycans (LPGs) and glycoinositolphospholipids (GIPLs) and infectivity to Cavia porcellus.
title_full_unstemmed Leishmania enriettii: biochemical characterisation of lipophosphoglycans (LPGs) and glycoinositolphospholipids (GIPLs) and infectivity to Cavia porcellus.
title_sort Leishmania enriettii: biochemical characterisation of lipophosphoglycans (LPGs) and glycoinositolphospholipids (GIPLs) and infectivity to Cavia porcellus.
author Paranaíba, Larissa Ferreira
author_facet Paranaíba, Larissa Ferreira
Assis, Rafael Ramiro de
Nogueira, Paula Monalisa
Torrecilhas, Ana Claúdia
Campos, João Henrique
Silveira, Amanda Cardoso de Oliveira
Martins Filho, Olindo Assis
Pessoa, Natalia Lima
Campos, Marco Antônio
Parreiras, Patrícia Martins
Melo, Maria Norma
Gontijo, Nelder de Figueiredo
Soares, Rodrigo Pedro Pinto
author_role author
author2 Assis, Rafael Ramiro de
Nogueira, Paula Monalisa
Torrecilhas, Ana Claúdia
Campos, João Henrique
Silveira, Amanda Cardoso de Oliveira
Martins Filho, Olindo Assis
Pessoa, Natalia Lima
Campos, Marco Antônio
Parreiras, Patrícia Martins
Melo, Maria Norma
Gontijo, Nelder de Figueiredo
Soares, Rodrigo Pedro Pinto
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Paranaíba, Larissa Ferreira
Assis, Rafael Ramiro de
Nogueira, Paula Monalisa
Torrecilhas, Ana Claúdia
Campos, João Henrique
Silveira, Amanda Cardoso de Oliveira
Martins Filho, Olindo Assis
Pessoa, Natalia Lima
Campos, Marco Antônio
Parreiras, Patrícia Martins
Melo, Maria Norma
Gontijo, Nelder de Figueiredo
Soares, Rodrigo Pedro Pinto
dc.subject.en.pt_BR.fl_str_mv Leishmania enriettii
Mice
polymorphisms
topic Leishmania enriettii
Mice
polymorphisms
description Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil/Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Laboratório de Fisiologia de Insetos Hematófagos. Belo Horizonte, MG, Brasil.
publishDate 2015
dc.date.issued.fl_str_mv 2015
dc.date.accessioned.fl_str_mv 2016-01-25T17:10:46Z
dc.date.available.fl_str_mv 2016-01-25T17:10:46Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.citation.fl_str_mv PARANAÍBA, Larissa Ferreira et al. Leishmania enriettii: biochemical characterisation of lipophosphoglycans (LPGs) and glycoinositolphospholipids (GIPLs) and infectivity to Cavia porcellus. Parasit Vectors., vol 8, p. 31, 2015
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/12606
dc.identifier.issn.none.fl_str_mv 1756-3305
dc.identifier.doi.none.fl_str_mv 10.1186/s13071-015-0633-8
identifier_str_mv PARANAÍBA, Larissa Ferreira et al. Leishmania enriettii: biochemical characterisation of lipophosphoglycans (LPGs) and glycoinositolphospholipids (GIPLs) and infectivity to Cavia porcellus. Parasit Vectors., vol 8, p. 31, 2015
1756-3305
10.1186/s13071-015-0633-8
url https://www.arca.fiocruz.br/handle/icict/12606
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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