Repeatability of the evaluation of systemic microvascular endothelial function using laser doppler perfusion monitoring: clinical and statistical implications

Detalhes bibliográficos
Autor(a) principal: Tibiriçá, Eduardo
Data de Publicação: 2011
Outros Autores: Matheus, Alessandra S. M., Nunes, Bruno, Sperandei, Sandro, Gomes, Marilia B.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/30210
Resumo: Universidade do Estado do Rio de Janeiro. Unidade de Diabetes. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Investigação Cardiovascular. Rio de Janeiro, RJ, Brasil.
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spelling Tibiriçá, EduardoMatheus, Alessandra S. M.Nunes, BrunoSperandei, SandroGomes, Marilia B.2018-11-26T19:19:43Z2018-11-26T19:19:43Z2011TIBIRIÇÁ, Eduardo: et al. Repeatability of the evaluation of systemic microvascular endothelial function using laser doppler perfusion monitoring: clinical and statistical implications. Clinicas, v.66, n.4, p.599-605, Jan. 2011.1807-5932https://www.arca.fiocruz.br/handle/icict/3021010.1590/S1807-593220110004000131980-5322engUniversidade de São Paulo. Faculdade de MedicinaMicrocirculação da PeleEstudos clínicosTamanho da amostraFluxometria por Doppler a LaserSkin MicrocirculationLaser Doppler FlowmetryClinical StudiesSample SizeRepeatability of the evaluation of systemic microvascular endothelial function using laser doppler perfusion monitoring: clinical and statistical implicationsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade do Estado do Rio de Janeiro. Unidade de Diabetes. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Investigação Cardiovascular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Investigação Cardiovascular. Rio de Janeiro, RJ, Brasil.Universidade do Estado do Rio de Janeiro. Unidade de Diabetes. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Investigação Cardiovascular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Computacional e de Sistemas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Investigação Cardiovascular. Rio de Janeiro, RJ, Brasil.OBJECTIVE: An awareness of the repeatability of biological measures is required to properly design and calculate sample sizes for longitudinal interventional studies. We investigated the day-to-day repeatability of measures of systemic microvascular reactivity using laser Doppler perfusion monitoring. METHODS: We performed laser Doppler perfusion monitoring in combination with skin iontophoresis using acetylcholine and sodium nitroprusside as well as post-occlusive reactive and thermal hyperemia twice within two weeks. The repeatability was assessed by calculating the within-subject standard deviations, limits of agreement, typical errors and intra-class correlation coefficients between days 1 and 2. The ratio of the within-subject standard deviation to the mean values obtained on days 1 and 2 (within-subject standard deviation/GM) was used to determine the condition with the best repeatability. RESULTS: Twenty-four healthy subjects, aged 24.6¡3.8 years, were recruited. The area under the curve of the vasodilatory response to post-occlusive reactivity showed marked variability (within-subject standard deviation/GM =0.83), while the area under the curve for acetylcholine exhibited less variability (within-subject standard deviation/ GM =0.52) and was comparable to the responses to sodium nitroprusside and thermal treatment (within-subject standard deviations/GM of 0.67 and 0.56, respectively). The area under the blood flow/time curve for vasodilation during acetylcholine administration required the smallest sample sizes, the area under the blood flow/time curve during post-occlusive reactivity required the largest sample sizes, and the area under the blood flow/time curves of vasodilation induced by sodium nitroprusside and thermal treatment required intermediate sizes. CONCLUSIONS: In view of the importance of random error related to the day-to-day repeatability of laser Doppler perfusion monitoring, we propose an original and robust statistical methodology for use in designing prospective clinical studies.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/30210/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALmarilia_gomes_tibiriça_etal_IOC_2011.pdfmarilia_gomes_tibiriça_etal_IOC_2011.pdfapplication/pdf201065https://www.arca.fiocruz.br/bitstream/icict/30210/2/marilia_gomes_tibiri%c3%a7a_etal_IOC_2011.pdfee702cf8308c6a68426cbcd1155f558eMD52TEXTmarilia_gomes_tibiriça_etal_IOC_2011.pdf.txtmarilia_gomes_tibiriça_etal_IOC_2011.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Repeatability of the evaluation of systemic microvascular endothelial function using laser doppler perfusion monitoring: clinical and statistical implications
title Repeatability of the evaluation of systemic microvascular endothelial function using laser doppler perfusion monitoring: clinical and statistical implications
spellingShingle Repeatability of the evaluation of systemic microvascular endothelial function using laser doppler perfusion monitoring: clinical and statistical implications
Tibiriçá, Eduardo
Microcirculação da Pele
Estudos clínicos
Tamanho da amostra
Fluxometria por Doppler a Laser
Skin Microcirculation
Laser Doppler Flowmetry
Clinical Studies
Sample Size
title_short Repeatability of the evaluation of systemic microvascular endothelial function using laser doppler perfusion monitoring: clinical and statistical implications
title_full Repeatability of the evaluation of systemic microvascular endothelial function using laser doppler perfusion monitoring: clinical and statistical implications
title_fullStr Repeatability of the evaluation of systemic microvascular endothelial function using laser doppler perfusion monitoring: clinical and statistical implications
title_full_unstemmed Repeatability of the evaluation of systemic microvascular endothelial function using laser doppler perfusion monitoring: clinical and statistical implications
title_sort Repeatability of the evaluation of systemic microvascular endothelial function using laser doppler perfusion monitoring: clinical and statistical implications
author Tibiriçá, Eduardo
author_facet Tibiriçá, Eduardo
Matheus, Alessandra S. M.
Nunes, Bruno
Sperandei, Sandro
Gomes, Marilia B.
author_role author
author2 Matheus, Alessandra S. M.
Nunes, Bruno
Sperandei, Sandro
Gomes, Marilia B.
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Tibiriçá, Eduardo
Matheus, Alessandra S. M.
Nunes, Bruno
Sperandei, Sandro
Gomes, Marilia B.
dc.subject.other.pt_BR.fl_str_mv Microcirculação da Pele
Estudos clínicos
Tamanho da amostra
Fluxometria por Doppler a Laser
topic Microcirculação da Pele
Estudos clínicos
Tamanho da amostra
Fluxometria por Doppler a Laser
Skin Microcirculation
Laser Doppler Flowmetry
Clinical Studies
Sample Size
dc.subject.en.pt_BR.fl_str_mv Skin Microcirculation
Laser Doppler Flowmetry
Clinical Studies
Sample Size
description Universidade do Estado do Rio de Janeiro. Unidade de Diabetes. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Investigação Cardiovascular. Rio de Janeiro, RJ, Brasil.
publishDate 2011
dc.date.issued.fl_str_mv 2011
dc.date.accessioned.fl_str_mv 2018-11-26T19:19:43Z
dc.date.available.fl_str_mv 2018-11-26T19:19:43Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv TIBIRIÇÁ, Eduardo: et al. Repeatability of the evaluation of systemic microvascular endothelial function using laser doppler perfusion monitoring: clinical and statistical implications. Clinicas, v.66, n.4, p.599-605, Jan. 2011.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/30210
dc.identifier.issn.pt_BR.fl_str_mv 1807-5932
dc.identifier.doi.none.fl_str_mv 10.1590/S1807-59322011000400013
dc.identifier.eissn.none.fl_str_mv 1980-5322
identifier_str_mv TIBIRIÇÁ, Eduardo: et al. Repeatability of the evaluation of systemic microvascular endothelial function using laser doppler perfusion monitoring: clinical and statistical implications. Clinicas, v.66, n.4, p.599-605, Jan. 2011.
1807-5932
10.1590/S1807-59322011000400013
1980-5322
url https://www.arca.fiocruz.br/handle/icict/30210
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Medicina
publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Medicina
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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