Rational design of large flat nitrogen-doped graphene oxide quantum dots with greenluminescence suitable for biomedical applications
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2022 |
| Outros Autores: | , , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/55185 |
Resumo: | Pontifícia Universidade Católica do Rio de Janeiro. Departamento de Química. Rio de Janeiro, RJ, Brasil. |
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Nazarkovsky, MichaelMikhraliieva, AlbinaAchete, Carlos A.Alves, Luiz AnastácioAraujo, JoyceArchanjo, Bráulio S.Barros, José Júnior França deCardoso, Liana Monteiro da FonsecaCouceiro, José Nelson S. S.Marques, Fernanda DaviOliveira, Bruno S.Souza, Rafael Nascimento Dias deTeixeira, Ayla JosmaVasconcelos, Thiago L.Zaitsev, Vladimir2022-10-17T20:53:08Z2022-10-17T20:53:08Z2022NAZARKOVSKY, Michael et al. Rational design of large flat nitrogen-doped graphene oxide quantum dots with greenluminescence suitable for biomedical applications. RSC Adv., v. 12, p. 14342-14355, 2022.2046-2069https://www.arca.fiocruz.br/handle/icict/5518510.1039/d2ra01516aengRoyal Society of ChemistryProjeto racionalGrandes planos dopados com nitrogênioPontos quânticos de óxido de grafenoLuminescência verdeAdequado para aplicações biomédicasRational design of large flat nitrogenDoped grapheneOxide quantum dotsWith greenluminescenceSuitable for biomedical applicationsRational design of large flat nitrogen-doped graphene oxide quantum dots with greenluminescence suitable for biomedical applicationsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlePontifícia Universidade Católica do Rio de Janeiro. Departamento de Química. Rio de Janeiro, RJ, Brasil.Pontifícia Universidade Católica do Rio de Janeiro. Departamento de Química. Rio de Janeiro, RJ, Brasil.Instituto Nacional de Metrologia, Qualidade e Tecnologia. Inmetro. Xerem, Duque de Caxias, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Comunicação Celular. Rio de Janeiro, RJ, Brasil.Instituto Nacional de Metrologia, Qualidade e Tecnologia. Inmetro. Xerem, Duque de Caxias, RJ, Brasil.Instituto Nacional de Metrologia, Qualidade e Tecnologia. Inmetro. Xerem, Duque de Caxias, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Molecular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Comunicação Celular. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Rio de Janeiro, RJ, Brasil.Instituto Nacional de Metrologia, Qualidade e Tecnologia. Inmetro. Xerem, Duque de Caxias, RJ, Brasil.Instituto Nacional de Metrologia, Qualidade e Tecnologia. Inmetro. Xerem, Duque de Caxias, RJ, Brasil.Instituto Nacional de Metrologia, Qualidade e Tecnologia. Inmetro. Xerem, Duque de Caxias, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Comunicação Celular. Rio de Janeiro, RJ, Brasil.Instituto Nacional de Metrologia, Qualidade e Tecnologia. Inmetro. Xerem, Duque de Caxias, RJ, Brasil.Pontifícia Universidade Católica do Rio de Janeiro. Departamento de Química. Rio de Janeiro, RJ, Brasil / National University of Kyiv-Mohyla Academy, 2 Skovorody Vul., Kyiv, 04070, Ukraine.Rational synthesis and simple methodology for the purification of large (35–45 nm in lateral size) and flat (1.0–1.5 nm of height) nitrogen-doped graphene oxide quantum dots (GOQDs) are presented. The methodology allows robust metal-free and acid-free preparation of N-GOQDs with a yield of about 100% and includes hydrothermal treatment of graphene oxide with hydrogen peroxide and ammonia. It was demonstrated that macroscopic impurities can be separated from N-GOQD suspension by their coagulation with 0.9% NaCl solution. Redispersible in water and saline solutions, particles of N-GOQDs were characterized using tip-enhanced Raman spectroscopy (TERS), photoluminescent, XPS, and UV-VIS spectroscopies. The size and morphology of N-GOQDs were studied by dynamic light scattering, AFM, SEM, and TEM. The procedure proposed allows nitrogen-doped GOQDs to be obtained, having 60–51% of carbon, 34–45% of oxygen, and up to 7.2% of nitrogen. The N-GOQD particles obtained in two hours of synthesis contain only pyrrolic defects of the graphene core. The fraction of pyridine moieties grows with the time of synthesis, while the fraction of quaternary nitrogen declines. Application of TERS allows demonstration that the N-GOQDs consist of a graphene core with an average crystallite size of 9 nm and an average distance between nearest defects smaller than 3 nm. The cytotoxicity tests reveal high viability of the monkey epithelial kidney cells Vero in the presence of N-GOQDs in a concentration below 60 mg L 1. The N-GOQDs demonstrate green luminescence with an emission maximum at 505 nm and sedimentation stability in the cell culture medium.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/55185/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALLianacardoso_JoseBarros_etal_IOC_2022.pdfLianacardoso_JoseBarros_etal_IOC_2022.pdfapplication/pdf2592227https://www.arca.fiocruz.br/bitstream/icict/55185/2/Lianacardoso_JoseBarros_etal_IOC_2022.pdf2aed0f1394c92d7af963bb39e65adcb6MD52icict/551852023-09-14 15:53:47.349oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-09-14T18:53:47Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.en_US.fl_str_mv |
Rational design of large flat nitrogen-doped graphene oxide quantum dots with greenluminescence suitable for biomedical applications |
| title |
Rational design of large flat nitrogen-doped graphene oxide quantum dots with greenluminescence suitable for biomedical applications |
| spellingShingle |
Rational design of large flat nitrogen-doped graphene oxide quantum dots with greenluminescence suitable for biomedical applications Nazarkovsky, Michael Projeto racional Grandes planos dopados com nitrogênio Pontos quânticos de óxido de grafeno Luminescência verde Adequado para aplicações biomédicas Rational design of large flat nitrogen Doped graphene Oxide quantum dots With greenluminescence Suitable for biomedical applications |
| title_short |
Rational design of large flat nitrogen-doped graphene oxide quantum dots with greenluminescence suitable for biomedical applications |
| title_full |
Rational design of large flat nitrogen-doped graphene oxide quantum dots with greenluminescence suitable for biomedical applications |
| title_fullStr |
Rational design of large flat nitrogen-doped graphene oxide quantum dots with greenluminescence suitable for biomedical applications |
| title_full_unstemmed |
Rational design of large flat nitrogen-doped graphene oxide quantum dots with greenluminescence suitable for biomedical applications |
| title_sort |
Rational design of large flat nitrogen-doped graphene oxide quantum dots with greenluminescence suitable for biomedical applications |
| author |
Nazarkovsky, Michael |
| author_facet |
Nazarkovsky, Michael Mikhraliieva, Albina Achete, Carlos A. Alves, Luiz Anastácio Araujo, Joyce Archanjo, Bráulio S. Barros, José Júnior França de Cardoso, Liana Monteiro da Fonseca Couceiro, José Nelson S. S. Marques, Fernanda Davi Oliveira, Bruno S. Souza, Rafael Nascimento Dias de Teixeira, Ayla Josma Vasconcelos, Thiago L. Zaitsev, Vladimir |
| author_role |
author |
| author2 |
Mikhraliieva, Albina Achete, Carlos A. Alves, Luiz Anastácio Araujo, Joyce Archanjo, Bráulio S. Barros, José Júnior França de Cardoso, Liana Monteiro da Fonseca Couceiro, José Nelson S. S. Marques, Fernanda Davi Oliveira, Bruno S. Souza, Rafael Nascimento Dias de Teixeira, Ayla Josma Vasconcelos, Thiago L. Zaitsev, Vladimir |
| author2_role |
author author author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Nazarkovsky, Michael Mikhraliieva, Albina Achete, Carlos A. Alves, Luiz Anastácio Araujo, Joyce Archanjo, Bráulio S. Barros, José Júnior França de Cardoso, Liana Monteiro da Fonseca Couceiro, José Nelson S. S. Marques, Fernanda Davi Oliveira, Bruno S. Souza, Rafael Nascimento Dias de Teixeira, Ayla Josma Vasconcelos, Thiago L. Zaitsev, Vladimir |
| dc.subject.other.en_US.fl_str_mv |
Projeto racional Grandes planos dopados com nitrogênio Pontos quânticos de óxido de grafeno Luminescência verde Adequado para aplicações biomédicas |
| topic |
Projeto racional Grandes planos dopados com nitrogênio Pontos quânticos de óxido de grafeno Luminescência verde Adequado para aplicações biomédicas Rational design of large flat nitrogen Doped graphene Oxide quantum dots With greenluminescence Suitable for biomedical applications |
| dc.subject.en.en_US.fl_str_mv |
Rational design of large flat nitrogen Doped graphene Oxide quantum dots With greenluminescence Suitable for biomedical applications |
| description |
Pontifícia Universidade Católica do Rio de Janeiro. Departamento de Química. Rio de Janeiro, RJ, Brasil. |
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2022 |
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2022-10-17T20:53:08Z |
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2022-10-17T20:53:08Z |
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2022 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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NAZARKOVSKY, Michael et al. Rational design of large flat nitrogen-doped graphene oxide quantum dots with greenluminescence suitable for biomedical applications. RSC Adv., v. 12, p. 14342-14355, 2022. |
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https://www.arca.fiocruz.br/handle/icict/55185 |
| dc.identifier.issn.en_US.fl_str_mv |
2046-2069 |
| dc.identifier.doi.none.fl_str_mv |
10.1039/d2ra01516a |
| identifier_str_mv |
NAZARKOVSKY, Michael et al. Rational design of large flat nitrogen-doped graphene oxide quantum dots with greenluminescence suitable for biomedical applications. RSC Adv., v. 12, p. 14342-14355, 2022. 2046-2069 10.1039/d2ra01516a |
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eng |
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eng |
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openAccess |
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Royal Society of Chemistry |
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Royal Society of Chemistry |
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