Analysis of a Routinely Used Commercial Anti-Chikungunya IgM ELISA Reveals Cross-Reactivities with Dengue in Brazil: A New Challenge for Differential Diagnosis?

Detalhes bibliográficos
Autor(a) principal: Lima, Monique da Rocha Queiroz
Data de Publicação: 2021
Outros Autores: Lima, Raquel Curtinhas de, Azevedo, Elzinandes Leal de, Santos, Flavia Barreto dos
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/48485
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ, Brasil.
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spelling Lima, Monique da Rocha QueirozLima, Raquel Curtinhas deAzevedo, Elzinandes Leal deSantos, Flavia Barreto dos2021-08-03T13:51:19Z2021-08-03T13:51:19Z2021LIMA, Monique da Rocha Queiroz et al. Analysis of a Routinely Used Commercial Anti-Chikungunya IgM ELISA Reveals Cross-Reactivities with Dengue in Brazil: A New Challenge for Differential Diagnosis?. Diagnostics, v. 11, 819, 11 p, Apr. 2021.2075-4418https://www.arca.fiocruz.br/handle/icict/4848510.3390/diagnostics11050819engMDPIDiagnóstico de chikunguniaAnti-chikungunia igM ELISAReatividade cruzada da dengueBrasilChikungunia diagnosisAnti-chikungunia igM ELISADengue cross-reactivityBrazilAnalysis of a Routinely Used Commercial Anti-Chikungunya IgM ELISA Reveals Cross-Reactivities with Dengue in Brazil: A New Challenge for Differential Diagnosis?info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ, Brasil.In Brazil, chikungunya emerged in 2014, and by 2016, co-circulated with other arbovirosis, such as dengue and zika. ELISAs (Enzyme-Linked Immunosorbent Assays) are the most widely used approach for arboviruses diagnosis. However, some limitations include antibody cross reactivities when viruses belong to the same genus, and sensitivity variations in distinct epidemiological scenarios. As chikungunya virus (CHIKV) is an alphavirus, no serological cross reactivity with dengue virus (DENV) should be observed. Here, we evaluated a routinely used chikungunya commercial IgM (Immunoglobulin M) ELISA test (Anti-Chikungunya IgM ELISA, Euroimmun) to assess its performance in confirming chikungunya in a dengue endemic area. Samples (n = 340) representative of all four DENV serotypes, healthy individuals and controls were tested. The Anti-CHIKV IgM ELISA test had a sensitivity of 100% and a specificity of 25.3% due to the cross reactivities observed with dengue. In dengue acute cases, the chikungunya test showed an overall cross-reactivity of 31.6%, with a higher cross-reactivity with DENV-4. In dengue IgM positive cases, the assay showed a cross-reactivity of 46.7%. Serological diagnosis may be challenging and, despite the results observed here, more evaluations shall be performed. Because distinct arboviruses co-circulate in Brazil, reliable diagnostic tools are essential for disease surveillance and patient management.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/48485/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALMoniqueRLima_etal_IOC_2021.pdfMoniqueRLima_etal_IOC_2021.pdfapplication/pdf643521https://www.arca.fiocruz.br/bitstream/icict/48485/2/MoniqueRLima_etal_IOC_2021.pdf8a28b081ab5b0c9a361880d36df66207MD52TEXTMoniqueRLima_etal_IOC_2021.pdf.txtMoniqueRLima_etal_IOC_2021.pdf.txtExtracted texttext/plain45186https://www.arca.fiocruz.br/bitstream/icict/48485/3/MoniqueRLima_etal_IOC_2021.pdf.txt0dbdb5480fc7d9cb829ba51b54c45ad0MD53icict/484852023-09-05 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dc.title.pt_BR.fl_str_mv Analysis of a Routinely Used Commercial Anti-Chikungunya IgM ELISA Reveals Cross-Reactivities with Dengue in Brazil: A New Challenge for Differential Diagnosis?
title Analysis of a Routinely Used Commercial Anti-Chikungunya IgM ELISA Reveals Cross-Reactivities with Dengue in Brazil: A New Challenge for Differential Diagnosis?
spellingShingle Analysis of a Routinely Used Commercial Anti-Chikungunya IgM ELISA Reveals Cross-Reactivities with Dengue in Brazil: A New Challenge for Differential Diagnosis?
Lima, Monique da Rocha Queiroz
Diagnóstico de chikungunia
Anti-chikungunia igM ELISA
Reatividade cruzada da dengue
Brasil
Chikungunia diagnosis
Anti-chikungunia igM ELISA
Dengue cross-reactivity
Brazil
title_short Analysis of a Routinely Used Commercial Anti-Chikungunya IgM ELISA Reveals Cross-Reactivities with Dengue in Brazil: A New Challenge for Differential Diagnosis?
title_full Analysis of a Routinely Used Commercial Anti-Chikungunya IgM ELISA Reveals Cross-Reactivities with Dengue in Brazil: A New Challenge for Differential Diagnosis?
title_fullStr Analysis of a Routinely Used Commercial Anti-Chikungunya IgM ELISA Reveals Cross-Reactivities with Dengue in Brazil: A New Challenge for Differential Diagnosis?
title_full_unstemmed Analysis of a Routinely Used Commercial Anti-Chikungunya IgM ELISA Reveals Cross-Reactivities with Dengue in Brazil: A New Challenge for Differential Diagnosis?
title_sort Analysis of a Routinely Used Commercial Anti-Chikungunya IgM ELISA Reveals Cross-Reactivities with Dengue in Brazil: A New Challenge for Differential Diagnosis?
author Lima, Monique da Rocha Queiroz
author_facet Lima, Monique da Rocha Queiroz
Lima, Raquel Curtinhas de
Azevedo, Elzinandes Leal de
Santos, Flavia Barreto dos
author_role author
author2 Lima, Raquel Curtinhas de
Azevedo, Elzinandes Leal de
Santos, Flavia Barreto dos
author2_role author
author
author
dc.contributor.author.fl_str_mv Lima, Monique da Rocha Queiroz
Lima, Raquel Curtinhas de
Azevedo, Elzinandes Leal de
Santos, Flavia Barreto dos
dc.subject.other.pt_BR.fl_str_mv Diagnóstico de chikungunia
Anti-chikungunia igM ELISA
Reatividade cruzada da dengue
Brasil
topic Diagnóstico de chikungunia
Anti-chikungunia igM ELISA
Reatividade cruzada da dengue
Brasil
Chikungunia diagnosis
Anti-chikungunia igM ELISA
Dengue cross-reactivity
Brazil
dc.subject.en.pt_BR.fl_str_mv Chikungunia diagnosis
Anti-chikungunia igM ELISA
Dengue cross-reactivity
Brazil
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ, Brasil.
publishDate 2021
dc.date.accessioned.fl_str_mv 2021-08-03T13:51:19Z
dc.date.available.fl_str_mv 2021-08-03T13:51:19Z
dc.date.issued.fl_str_mv 2021
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv LIMA, Monique da Rocha Queiroz et al. Analysis of a Routinely Used Commercial Anti-Chikungunya IgM ELISA Reveals Cross-Reactivities with Dengue in Brazil: A New Challenge for Differential Diagnosis?. Diagnostics, v. 11, 819, 11 p, Apr. 2021.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/48485
dc.identifier.issn.pt_BR.fl_str_mv 2075-4418
dc.identifier.doi.none.fl_str_mv 10.3390/diagnostics11050819
identifier_str_mv LIMA, Monique da Rocha Queiroz et al. Analysis of a Routinely Used Commercial Anti-Chikungunya IgM ELISA Reveals Cross-Reactivities with Dengue in Brazil: A New Challenge for Differential Diagnosis?. Diagnostics, v. 11, 819, 11 p, Apr. 2021.
2075-4418
10.3390/diagnostics11050819
url https://www.arca.fiocruz.br/handle/icict/48485
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/48485/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/48485/2/MoniqueRLima_etal_IOC_2021.pdf
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