Mycobacterium tuberculosis Rv1419 encodes a secreted 13 kDa lectin with immunological reactivity during human tuberculosis
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2010 |
| Outros Autores: | , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/7386 |
Resumo: | Universidade Federal de Santa Catarina. Departamento de Microbiologia e Parasitologia. Laboratório de Imunologia e Doenças Infecciosas. Florianópolis, SC, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório Integrado de Microbiologia e Imunoregulação. Salvador, BA, Brasil |
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Nogueira, Lucas LCardoso, Fernanda CMattos, Ana MBordignon, JulianoFigueiredo, Cláudia PDahlstrom, PedroFrota, Cristiane CSantos, Cláudia N. Duarte dosChalhoub, MarceloCavada, Benildo SousaTeixeira, Henrique COliveira, Sérgio CostaBarral Netto, ManoelBafica, Andre Luiz Barbosa2014-03-10T14:23:01Z2014-03-10T14:23:01Z2010NOGUEIRA, L. et al. Mycobacterium tuberculosis Rv1419 encodes a secreted 13 kDa lectin with immunological reactivity during human tuberculosis. European Journal of Immunology, v. 40, p. 744-753, 2010.1521-4141https://www.arca.fiocruz.br/handle/icict/738610.1002/eji.200939747engWILEY-VCH Verlag GmbH & Co. KGaA, WeinheimMycobacterium tuberculosis Rv1419 encodes a secreted 13 kDa lectin with immunological reactivity during human tuberculosisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de Santa Catarina. Departamento de Microbiologia e Parasitologia. Laboratório de Imunologia e Doenças Infecciosas. Florianópolis, SC, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório Integrado de Microbiologia e Imunoregulação. Salvador, BA, BrasilUniversidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, BrasilUniversidade Federal de Juiz de Fora. Laboratório de Imunologia. Juiz de Fora, MG, BrasilFundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, BrasilUniversiade Federal de Santa Catarina. Departamento de Farmacologia. Florianópolis, SC BrasilUniversidade Federal de Santa Catarina. Departamento de Microbiologia e Parasitologia. Laboratório de Imunologia e Doenças Infecciosas. Florianópolis, SC, BrasilUniversidade Federal do Ceará. BioMol-Lab. Fortaleza, CE, BrasilFundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, BrasilHospital Octávio Mangabeira. Salvador, BA, BrasilUniversidade Federal do Ceará. BioMol-Lab. Fortaleza, CE, BrasilUniversidade Federal de Juiz de Fora. Laboratório de Imunologia. Juiz de Fora, MG, BrasilUniversidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório Integrado de Microbiologia e Imunoregulação. Salvador, BA, BrasilUniversidade Federal de Santa Catarina. Departamento de Microbiologia e Parasitologia. Laboratório de Imunologia e Doenças Infecciosas. Florianópolis, SC, BrasilIn this study, we have identified a secreted 13 kDa lectin from Mtb (Mtb, Mycobacterium tuberculosis; sMTL-13) by homology search of a non-redundant lectin database. Bioinformatic analysis revealed that sMTL-13 belongs to the ricin-type beta-trefoil family of proteins containing a Sec-type signal peptide present in Mtb complex species, but not in non-tuberculous mycobacteria. Following heterologous expression of sMTL-13 and generation of an mAb (clone 276.B7/IgG1kappa), we confirmed that this lectin is present in culture filtrate proteins from Mtb H37Rv, but not in non-tuberculous mycobacteria-derived culture filtrate proteins. In addition, sMTL-13 leads to an increased IFN-gamma production by PBMC from active tuberculosis (ATB) patients. Furthermore, sera from ATB patients displayed high titers of IgG Ab against sMTL-13, a response found to be decreased following successful anti-tuberculosis therapy. Together, our findings reveal a secreted 13 kDa ricin-like lectin from Mtb, which is immunologically recognized during ATB and could serve as a biomarker of disease treatment.BiomarkersLectinMycobacterium tuberculosisAntígenos de Bactérias/imunologiaProteínas de Bactérias/imunologiaLectinas/imunologiaMycobacterium tuberculosis/imunologiaTuberculose Pulmonar/imunologiaSequência de AminoácidosAnticorpos Antibacterianos/genéticaAnticorpos Antibacterianos/imunologiaAntígenos de Bactérias/genéticaAntígenos de Bactérias/metabolismoProteínas de Bactérias/genéticaProteínas de Bactérias/metabolismoSequência de BasesWestern BlottingHumanosImunoglobulina G/imunologiaImuno-HistoquímicaLectinas/genéticaLectinas/metabolismoDados de Sequência MolecularMycobacterium tuberculosis/genéticaMycobacterium tuberculosis/metabolismoTuberculose Pulmonar/genéticaTuberculose Pulmonar/metabolismoinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALNogueira L Mycrobacterium tuberculosis RV1419....pdfNogueira L Mycrobacterium tuberculosis RV1419....pdfapplication/pdf392843https://www.arca.fiocruz.br/bitstream/icict/7386/1/Nogueira%20L%20Mycrobacterium%20tuberculosis%20RV1419....pdfbc4929b39c77b71bf675eebf64400722MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/7386/2/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD52TEXTNogueira L Mycrobacterium tuberculosis RV1419....pdf.txtNogueira L Mycrobacterium tuberculosis RV1419....pdf.txtExtracted texttext/plain44389https://www.arca.fiocruz.br/bitstream/icict/7386/5/Nogueira%20L%20Mycrobacterium%20tuberculosis%20RV1419....pdf.txt2b3b59e905fe2fc41f6a76e276a80ebdMD55THUMBNAILNogueira L Mycrobacterium tuberculosis RV1419....pdf.jpgNogueira L Mycrobacterium tuberculosis RV1419....pdf.jpgGenerated Thumbnailimage/jpeg2256https://www.arca.fiocruz.br/bitstream/icict/7386/4/Nogueira%20L%20Mycrobacterium%20tuberculosis%20RV1419....pdf.jpg74d8b449acceb5c42c5bf14f8792ac68MD54icict/73862023-03-15 14:33:27.184oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:27Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.pt_BR.fl_str_mv |
Mycobacterium tuberculosis Rv1419 encodes a secreted 13 kDa lectin with immunological reactivity during human tuberculosis |
| title |
Mycobacterium tuberculosis Rv1419 encodes a secreted 13 kDa lectin with immunological reactivity during human tuberculosis |
| spellingShingle |
Mycobacterium tuberculosis Rv1419 encodes a secreted 13 kDa lectin with immunological reactivity during human tuberculosis Nogueira, Lucas L Biomarkers Lectin Mycobacterium tuberculosis Antígenos de Bactérias/imunologia Proteínas de Bactérias/imunologia Lectinas/imunologia Mycobacterium tuberculosis/imunologia Tuberculose Pulmonar/imunologia Sequência de Aminoácidos Anticorpos Antibacterianos/genética Anticorpos Antibacterianos/imunologia Antígenos de Bactérias/genética Antígenos de Bactérias/metabolismo Proteínas de Bactérias/genética Proteínas de Bactérias/metabolismo Sequência de Bases Western Blotting Humanos Imunoglobulina G/imunologia Imuno-Histoquímica Lectinas/genética Lectinas/metabolismo Dados de Sequência Molecular Mycobacterium tuberculosis/genética Mycobacterium tuberculosis/metabolismo Tuberculose Pulmonar/genética Tuberculose Pulmonar/metabolismo |
| title_short |
Mycobacterium tuberculosis Rv1419 encodes a secreted 13 kDa lectin with immunological reactivity during human tuberculosis |
| title_full |
Mycobacterium tuberculosis Rv1419 encodes a secreted 13 kDa lectin with immunological reactivity during human tuberculosis |
| title_fullStr |
Mycobacterium tuberculosis Rv1419 encodes a secreted 13 kDa lectin with immunological reactivity during human tuberculosis |
| title_full_unstemmed |
Mycobacterium tuberculosis Rv1419 encodes a secreted 13 kDa lectin with immunological reactivity during human tuberculosis |
| title_sort |
Mycobacterium tuberculosis Rv1419 encodes a secreted 13 kDa lectin with immunological reactivity during human tuberculosis |
| author |
Nogueira, Lucas L |
| author_facet |
Nogueira, Lucas L Cardoso, Fernanda C Mattos, Ana M Bordignon, Juliano Figueiredo, Cláudia P Dahlstrom, Pedro Frota, Cristiane C Santos, Cláudia N. Duarte dos Chalhoub, Marcelo Cavada, Benildo Sousa Teixeira, Henrique C Oliveira, Sérgio Costa Barral Netto, Manoel Bafica, Andre Luiz Barbosa |
| author_role |
author |
| author2 |
Cardoso, Fernanda C Mattos, Ana M Bordignon, Juliano Figueiredo, Cláudia P Dahlstrom, Pedro Frota, Cristiane C Santos, Cláudia N. Duarte dos Chalhoub, Marcelo Cavada, Benildo Sousa Teixeira, Henrique C Oliveira, Sérgio Costa Barral Netto, Manoel Bafica, Andre Luiz Barbosa |
| author2_role |
author author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Nogueira, Lucas L Cardoso, Fernanda C Mattos, Ana M Bordignon, Juliano Figueiredo, Cláudia P Dahlstrom, Pedro Frota, Cristiane C Santos, Cláudia N. Duarte dos Chalhoub, Marcelo Cavada, Benildo Sousa Teixeira, Henrique C Oliveira, Sérgio Costa Barral Netto, Manoel Bafica, Andre Luiz Barbosa |
| dc.subject.en.pt_BR.fl_str_mv |
Biomarkers Lectin Mycobacterium tuberculosis |
| topic |
Biomarkers Lectin Mycobacterium tuberculosis Antígenos de Bactérias/imunologia Proteínas de Bactérias/imunologia Lectinas/imunologia Mycobacterium tuberculosis/imunologia Tuberculose Pulmonar/imunologia Sequência de Aminoácidos Anticorpos Antibacterianos/genética Anticorpos Antibacterianos/imunologia Antígenos de Bactérias/genética Antígenos de Bactérias/metabolismo Proteínas de Bactérias/genética Proteínas de Bactérias/metabolismo Sequência de Bases Western Blotting Humanos Imunoglobulina G/imunologia Imuno-Histoquímica Lectinas/genética Lectinas/metabolismo Dados de Sequência Molecular Mycobacterium tuberculosis/genética Mycobacterium tuberculosis/metabolismo Tuberculose Pulmonar/genética Tuberculose Pulmonar/metabolismo |
| dc.subject.decs.pt_BR.fl_str_mv |
Antígenos de Bactérias/imunologia Proteínas de Bactérias/imunologia Lectinas/imunologia Mycobacterium tuberculosis/imunologia Tuberculose Pulmonar/imunologia Sequência de Aminoácidos Anticorpos Antibacterianos/genética Anticorpos Antibacterianos/imunologia Antígenos de Bactérias/genética Antígenos de Bactérias/metabolismo Proteínas de Bactérias/genética Proteínas de Bactérias/metabolismo Sequência de Bases Western Blotting Humanos Imunoglobulina G/imunologia Imuno-Histoquímica Lectinas/genética Lectinas/metabolismo Dados de Sequência Molecular Mycobacterium tuberculosis/genética Mycobacterium tuberculosis/metabolismo Tuberculose Pulmonar/genética Tuberculose Pulmonar/metabolismo |
| description |
Universidade Federal de Santa Catarina. Departamento de Microbiologia e Parasitologia. Laboratório de Imunologia e Doenças Infecciosas. Florianópolis, SC, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório Integrado de Microbiologia e Imunoregulação. Salvador, BA, Brasil |
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2010 |
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2010 |
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2014-03-10T14:23:01Z |
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2014-03-10T14:23:01Z |
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NOGUEIRA, L. et al. Mycobacterium tuberculosis Rv1419 encodes a secreted 13 kDa lectin with immunological reactivity during human tuberculosis. European Journal of Immunology, v. 40, p. 744-753, 2010. |
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https://www.arca.fiocruz.br/handle/icict/7386 |
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1521-4141 |
| dc.identifier.doi.none.fl_str_mv |
10.1002/eji.200939747 |
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NOGUEIRA, L. et al. Mycobacterium tuberculosis Rv1419 encodes a secreted 13 kDa lectin with immunological reactivity during human tuberculosis. European Journal of Immunology, v. 40, p. 744-753, 2010. 1521-4141 10.1002/eji.200939747 |
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WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim |
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WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim |
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