Comparative proteomic analysis of antimony-resistant and -susceptible Leishmania braziliensis and Leishmania infantum chagasi lines

Detalhes bibliográficos
Autor(a) principal: Matrangolo, Fabiana da Silva Vieira
Data de Publicação: 2013
Outros Autores: Liarte, Daniel Barbosa, Andrade, Laila de Carvalho, Melo, Melina Ferreira de, Andrade, Juvana Moreira, Ferreira, Rafael Fernandes, Santiago, André da Silva, Pirovani, Carlos Priminho, Pereira, Rosiane Aparecida da Silva, Murta, Silvane Maria Fonseca
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
DOI: 10.26719/2012.18.2.165
Texto Completo: https://www.arca.fiocruz.br/handle/icict/30326
Resumo: 2020-01-01
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spelling Matrangolo, Fabiana da Silva VieiraLiarte, Daniel BarbosaAndrade, Laila de CarvalhoMelo, Melina Ferreira deAndrade, Juvana MoreiraFerreira, Rafael FernandesSantiago, André da SilvaPirovani, Carlos PriminhoPereira, Rosiane Aparecida da SilvaMurta, Silvane Maria Fonseca2018-11-29T15:15:14Z2018-11-29T15:15:14Z2013MATRANGOLO, Fabiana da Silva Vieira et al. Comparative proteomic analysis of antimony-resistant and -susceptible Leishmania braziliensis and Leishmania infantum chagasi lines. Molecular & Biochemical Parasitology, v. 190, p. 63– 75, 20130166-6851https://www.arca.fiocruz.br/handle/icict/3032610.26719/2012.18.2.165engElsevierLeishmania braziliensisLeishmania infantum chagasiresistencia a antimonioproteoma2-DELeishmania braziliensisLeishmania infantum chagasiAntimony resistanceProteome2-DEComparative proteomic analysis of antimony-resistant and -susceptible Leishmania braziliensis and Leishmania infantum chagasi linesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2020-01-01Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Parasitologia Celular e Molecular. Belo Horizonte, MG, Brazil/Universidade Estadual de Montes Claros. Laboratório de Epidemiologia e Biocontrole de Microrganismos. Montes Claros, BrazilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Parasitologia Celular e Molecular. Belo Horizonte, MG, Brazil/ Universidade Federal do Piauí. Piauí, BrazilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Parasitologia Celular e Molecular. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Parasitologia Celular e Molecular. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Parasitologia Celular e Molecular. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Parasitologia Celular e Molecular. Belo Horizonte, MG, BrazilUniversidade Estadual de Santa Cruz. Laboratório de Proteômica. Ilheús, BA, BrazilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Parasitologia Celular e Molecular. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Parasitologia Celular e Molecular. Belo Horizonte, MG, BrazilThe emergence of drug-resistant Leishmania species is a significant problem in several countries. A comparative proteomic analysis of antimony-susceptible and antimony-resistant Leishmania braziliensis (LbSbR) and Leishmania infantum chagasi (LcSbR) lines was carried out using two-dimensional gel electrophoresis (2-DE) followed by mass spectrometry (LC/MS/MS) for protein identification. Out of 132 protein spots exclusive or up-regulated submitted to MS, we identified 80 proteins that corresponded to 57 distinct proteins. Comparative analysis of data showed that most of the protein spots with differential abundance in both species are involved in antioxidant defense, general stress response, glucose and amino acid metabolism, and cytoskeleton organization. Five proteins were commonly more abundant in both SbIII-resistant Leishmania lines: tryparedoxin peroxidase, alpha-tubulin, HSP70, HSP83, and HSP60. Analysis of the protein abundance by Western blotting assays confirmed our proteomic data. These assays revealed that cyclophilin-A is less expressed in both LbSbR and LcSbR lines. On the other hand, the expression of pteridine reductase is higher in the LbSbR line, whereas tryparedoxin peroxidase is overexpressed in both LbSbR and LcSbR lines. Together, these results show that the mechanism of antimony-resistance in Leishmania spp. is complex and multifactorial.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83082https://www.arca.fiocruz.br/bitstream/icict/30326/1/license.txt9193a7c197bc67acd023525e72a03240MD51ORIGINALComparative proteomic analysis.pdfComparative proteomic analysis.pdfapplication/pdf755443https://www.arca.fiocruz.br/bitstream/icict/30326/2/Comparative%20proteomic%20analysis.pdfd8263071ad3408c75dc20b939e54e0acMD52TEXTComparative proteomic analysis.pdf.txtComparative proteomic analysis.pdf.txtExtracted texttext/plain68418https://www.arca.fiocruz.br/bitstream/icict/30326/3/Comparative%20proteomic%20analysis.pdf.txt0be0a84478f32c101ef803522c136f20MD53icict/303262021-03-24 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dc.title.pt_BR.fl_str_mv Comparative proteomic analysis of antimony-resistant and -susceptible Leishmania braziliensis and Leishmania infantum chagasi lines
title Comparative proteomic analysis of antimony-resistant and -susceptible Leishmania braziliensis and Leishmania infantum chagasi lines
spellingShingle Comparative proteomic analysis of antimony-resistant and -susceptible Leishmania braziliensis and Leishmania infantum chagasi lines
Matrangolo, Fabiana da Silva Vieira
Leishmania braziliensis
Leishmania infantum chagasi
resistencia a antimonio
proteoma
2-DE
Leishmania braziliensis
Leishmania infantum chagasi
Antimony resistance
Proteome
2-DE
title_short Comparative proteomic analysis of antimony-resistant and -susceptible Leishmania braziliensis and Leishmania infantum chagasi lines
title_full Comparative proteomic analysis of antimony-resistant and -susceptible Leishmania braziliensis and Leishmania infantum chagasi lines
title_fullStr Comparative proteomic analysis of antimony-resistant and -susceptible Leishmania braziliensis and Leishmania infantum chagasi lines
title_full_unstemmed Comparative proteomic analysis of antimony-resistant and -susceptible Leishmania braziliensis and Leishmania infantum chagasi lines
title_sort Comparative proteomic analysis of antimony-resistant and -susceptible Leishmania braziliensis and Leishmania infantum chagasi lines
author Matrangolo, Fabiana da Silva Vieira
author_facet Matrangolo, Fabiana da Silva Vieira
Liarte, Daniel Barbosa
Andrade, Laila de Carvalho
Melo, Melina Ferreira de
Andrade, Juvana Moreira
Ferreira, Rafael Fernandes
Santiago, André da Silva
Pirovani, Carlos Priminho
Pereira, Rosiane Aparecida da Silva
Murta, Silvane Maria Fonseca
author_role author
author2 Liarte, Daniel Barbosa
Andrade, Laila de Carvalho
Melo, Melina Ferreira de
Andrade, Juvana Moreira
Ferreira, Rafael Fernandes
Santiago, André da Silva
Pirovani, Carlos Priminho
Pereira, Rosiane Aparecida da Silva
Murta, Silvane Maria Fonseca
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Matrangolo, Fabiana da Silva Vieira
Liarte, Daniel Barbosa
Andrade, Laila de Carvalho
Melo, Melina Ferreira de
Andrade, Juvana Moreira
Ferreira, Rafael Fernandes
Santiago, André da Silva
Pirovani, Carlos Priminho
Pereira, Rosiane Aparecida da Silva
Murta, Silvane Maria Fonseca
dc.subject.other.pt_BR.fl_str_mv Leishmania braziliensis
Leishmania infantum chagasi
resistencia a antimonio
proteoma
2-DE
topic Leishmania braziliensis
Leishmania infantum chagasi
resistencia a antimonio
proteoma
2-DE
Leishmania braziliensis
Leishmania infantum chagasi
Antimony resistance
Proteome
2-DE
dc.subject.en.pt_BR.fl_str_mv Leishmania braziliensis
Leishmania infantum chagasi
Antimony resistance
Proteome
2-DE
description 2020-01-01
publishDate 2013
dc.date.issued.fl_str_mv 2013
dc.date.accessioned.fl_str_mv 2018-11-29T15:15:14Z
dc.date.available.fl_str_mv 2018-11-29T15:15:14Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv MATRANGOLO, Fabiana da Silva Vieira et al. Comparative proteomic analysis of antimony-resistant and -susceptible Leishmania braziliensis and Leishmania infantum chagasi lines. Molecular & Biochemical Parasitology, v. 190, p. 63– 75, 2013
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/30326
dc.identifier.issn.pt_BR.fl_str_mv 0166-6851
dc.identifier.doi.none.fl_str_mv 10.26719/2012.18.2.165
identifier_str_mv MATRANGOLO, Fabiana da Silva Vieira et al. Comparative proteomic analysis of antimony-resistant and -susceptible Leishmania braziliensis and Leishmania infantum chagasi lines. Molecular & Biochemical Parasitology, v. 190, p. 63– 75, 2013
0166-6851
10.26719/2012.18.2.165
url https://www.arca.fiocruz.br/handle/icict/30326
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
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