Spontaneous cystogenesis of Toxoplasma gondii in feline epithelial cells in vitro

Detalhes bibliográficos
Autor(a) principal: Muno, Renata M. de
Data de Publicação: 2014
Outros Autores: Moura, Marcos A, Carvalho, Luciany C. de, Seabra, Sergio H., Barbosa, Helene S
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/10217
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Estrutural. Rio de Janeiro, RJ, Brasil.
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spelling Muno, Renata M. deMoura, Marcos ACarvalho, Luciany C. deSeabra, Sergio H.Barbosa, Helene S2015-05-04T17:07:32Z2015-05-04T17:07:32Z2014MUNO, Renata M. de et al. Spontaneous cystogenesis of Toxoplasma gondii in feline epithelial cells in vitro. Folhia Parasitologica, v.62, n.2, p.113-119, 2014.0015-58631803-6465https://www.arca.fiocruz.br/handle/icict/1021710.14411/fp.2014.017engActaviaToxoplasmoseBradyzoitesFelids cellsTachyzoitesTissue cysts in vitroToxoplasmosisToxoplasmosisCiências BiológicasToxoplasmoseSpontaneous cystogenesis of Toxoplasma gondii in feline epithelial cells in vitroinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Estrutural. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Estrutural. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Estrutural. Rio de Janeiro, RJ, Brasil.Centro Universitário Estadual da Zona Oeste. Laboratório de Tecnologia em Cultura de Células. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Estrutural. Rio de Janeiro, RJ, Brasil.Toxoplasma gondii Nicolle et Manceaux, 1908 is an obligate intracellular parasite with the ability to infect mammals and birds. The only definitive hosts for T. gondii are felids, as the parasites form immature oocysts that are shed in the faeces. Here we introduce cat cells as a model for the study of experimental toxoplasmosis. We selected epithelial cells derived from cat kidneys (CR FK) as a target to determine the intracellular fate of bradyzoites of the T. gondii ME49 strain. In parallel, we compared this infection using epithelial cells from the rat intestine (IEC-6), considering the enteroepithelial development that occurs in the cat. Different ratios of parasites to host cells were assayed over the course of a 14-day-infection. The intracellular development of T. gondii was dependent on the source of the epithelial cells and also on the parasite/host cell ratio. Cystogenesis was well established in the CR FK cell line at a ratio of 1 : 10 after 10–14 days of infection. This cellular model system opens a new field of investigation into the molecular aspects of the interactions between T. gondii and feline epithelial cells. The CR FK cell line appears to be a potential cellular model for large scale cyst production in vitro, which would allow a reduction in the number of animals used and/or replacement of animals by in vitro cultures.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1914https://www.arca.fiocruz.br/bitstream/icict/10217/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALrenata_munoetal_IOC_2014.pdfapplication/pdf4655416https://www.arca.fiocruz.br/bitstream/icict/10217/2/renata_munoetal_IOC_2014.pdfb4dfed4881f110e689823bce1561677fMD52TEXTrenata_munoetal_IOC_2014.pdf.txtrenata_munoetal_IOC_2014.pdf.txtExtracted texttext/plain36062https://www.arca.fiocruz.br/bitstream/icict/10217/3/renata_munoetal_IOC_2014.pdf.txt7de94603d2825b345ecb91c73fa0c9dfMD53icict/102172022-06-24 13:08:50.3oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352022-06-24T16:08:50Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Spontaneous cystogenesis of Toxoplasma gondii in feline epithelial cells in vitro
title Spontaneous cystogenesis of Toxoplasma gondii in feline epithelial cells in vitro
spellingShingle Spontaneous cystogenesis of Toxoplasma gondii in feline epithelial cells in vitro
Muno, Renata M. de
Toxoplasmose
Bradyzoites
Felids cells
Tachyzoites
Tissue cysts in vitro
Toxoplasmosis
Toxoplasmosis
Ciências Biológicas
Toxoplasmose
title_short Spontaneous cystogenesis of Toxoplasma gondii in feline epithelial cells in vitro
title_full Spontaneous cystogenesis of Toxoplasma gondii in feline epithelial cells in vitro
title_fullStr Spontaneous cystogenesis of Toxoplasma gondii in feline epithelial cells in vitro
title_full_unstemmed Spontaneous cystogenesis of Toxoplasma gondii in feline epithelial cells in vitro
title_sort Spontaneous cystogenesis of Toxoplasma gondii in feline epithelial cells in vitro
author Muno, Renata M. de
author_facet Muno, Renata M. de
Moura, Marcos A
Carvalho, Luciany C. de
Seabra, Sergio H.
Barbosa, Helene S
author_role author
author2 Moura, Marcos A
Carvalho, Luciany C. de
Seabra, Sergio H.
Barbosa, Helene S
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Muno, Renata M. de
Moura, Marcos A
Carvalho, Luciany C. de
Seabra, Sergio H.
Barbosa, Helene S
dc.subject.other.pt_BR.fl_str_mv Toxoplasmose
topic Toxoplasmose
Bradyzoites
Felids cells
Tachyzoites
Tissue cysts in vitro
Toxoplasmosis
Toxoplasmosis
Ciências Biológicas
Toxoplasmose
dc.subject.en.pt_BR.fl_str_mv Bradyzoites
Felids cells
Tachyzoites
Tissue cysts in vitro
Toxoplasmosis
dc.subject.es.pt_BR.fl_str_mv Toxoplasmosis
dc.subject.decs.pt_BR.fl_str_mv Ciências Biológicas
Toxoplasmose
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Estrutural. Rio de Janeiro, RJ, Brasil.
publishDate 2014
dc.date.issued.fl_str_mv 2014
dc.date.accessioned.fl_str_mv 2015-05-04T17:07:32Z
dc.date.available.fl_str_mv 2015-05-04T17:07:32Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv MUNO, Renata M. de et al. Spontaneous cystogenesis of Toxoplasma gondii in feline epithelial cells in vitro. Folhia Parasitologica, v.62, n.2, p.113-119, 2014.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/10217
dc.identifier.issn.pt_BR.fl_str_mv 0015-5863
1803-6465
dc.identifier.doi.pt_BR.fl_str_mv 10.14411/fp.2014.017
identifier_str_mv MUNO, Renata M. de et al. Spontaneous cystogenesis of Toxoplasma gondii in feline epithelial cells in vitro. Folhia Parasitologica, v.62, n.2, p.113-119, 2014.
0015-5863
1803-6465
10.14411/fp.2014.017
url https://www.arca.fiocruz.br/handle/icict/10217
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Actavia
publisher.none.fl_str_mv Actavia
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
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instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
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