Multidisciplinary approach detects speciation within the kissing bug Panstrongylus rufotuberculatus populations (Hemiptera, Heteroptera, Reduviidae)

Detalhes bibliográficos
Autor(a) principal: Pita, Sebastián
Data de Publicação: 2021
Outros Autores: Gómez-Palacio, Andrés, Lorite, Pedro, Dujardim, Jean Pierre, Chavez, Tamara, Villacís, Anita G., Galvão, Cleber, Calleros, Lucía, Pereyra-Mello, Santiago, Burgueño-Rodríguez, Gabriela, Panzera, Francisco
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/51314
Resumo: Universidad de la República. Facultad de Ciencias. Sección Genética Evolutiva. Montevideo, Uruguay.
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spelling Pita, SebastiánGómez-Palacio, AndrésLorite, PedroDujardim, Jean PierreChavez, TamaraVillacís, Anita G.Galvão, CleberCalleros, LucíaPereyra-Mello, SantiagoBurgueño-Rodríguez, GabrielaPanzera, Francisco2022-02-19T20:01:45Z2022-02-19T20:01:45Z2021PITA, Sebastián et al. Multidisciplinary approach detects speciation within the kissing bug Panstrongylus rufotuberculatus populations (Hemiptera, Heteroptera, Reduviidae). Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 116, e210259, p. 1 - 12, 2021.0074-0206https://www.arca.fiocruz.br/handle/icict/5131410.1590/0074-02760210259engFundação Oswaldo Cruz. Instituto Oswaldo Cruz.Vetores da doença de ChagasGene do citocromo bSubunidade I do citocromo C oxidaseEspaçador transcrito interno 2Evolução do cariótipoAnálises morfométricasFusão dos cromossomos sexuaisChagas disease vectorsCytochrome b geneCytochrome C oxidase subunit IInternal transcribed spacer 2Karyotype evolutionMorphometric analysesSex chromosome fusionMultidisciplinary approach detects speciation within the kissing bug Panstrongylus rufotuberculatus populations (Hemiptera, Heteroptera, Reduviidae)info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidad de la República. Facultad de Ciencias. Sección Genética Evolutiva. Montevideo, Uruguay.Universidad Pedagógica y Tecnológica de Colombia. Laboratorio de Investigación en Genética Evolutiva. Boyacá. Colombia.Universidad de Jaén. Departamento de Biología Experimental. Área de Genética. Jaén, Spain.University of Montpellier. Institut de Recherche pour le Développement. International Campus in Baillarguet. Montpellier, Occitanie, France.Instituto Nacional de Laboratorios de Salud. Laboratorio de Entomología Médica. La Paz, Bolivia.Pontificia Universidad Católica del Ecuador. Facultad de Ciencias Exactas y Naturales. Escuela de Ciencias Biológicas. Centro de Investigación para la Salud en América Latina. Nayón, Quito, Ecuador.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Nacional e Internacional de Referência em Taxonomia de Triatomíneos. Rio de Janeiro, RJ, Brasil.Universidad de la República. Facultad de Ciencias. Sección Genética Evolutiva. Montevideo, Uruguay.Universidad de la República. Facultad de Ciencias. Sección Genética Evolutiva. Montevideo, Uruguay.Universidad de la República. Facultad de Ciencias. Sección Genética Evolutiva. Montevideo, Uruguay.Universidad de la República. Centro Universitario Regional. Departamento de Ciencias Biológicas. Laboratorio de Genética Molecular Humana. Salto, Uruguay.Universidad de la República. Facultad de Ciencias. Sección Genética Evolutiva. Montevideo, Uruguay.BACKGROUND Panstrongylus rufotuberculatus (Hemiptera-Reduviidae) is a triatomine species with a wide geographic distribution and a broad phenotypic variability. In some countries, this species is found infesting and colonising domiciliary ecotopes representing an epidemiological risk factor as a vector of Trypanosoma cruzi, etiological agent of Chagas disease. In spite of this, little is known about P. rufotuberculatus genetic diversity. METHODS Cytogenetic studies and DNA sequence analyses of one nuclear (ITS-2) and two mitochondrial DNA sequences (cyt b and coI) were carried out in P. rufotuberculatus individuals collected in Bolivia, Colombia, Ecuador and Mexico. Moreover, a geometric morphometrics study was applied to Bolivian, Colombian, Ecuadorian and French Guiana samples. OBJECTIVES To explore the genetic and phenetic diversity of P. rufotuberculatus from different countries, combining chromosomal studies, DNA sequence analyses and geometric morphometric comparisons. FINDINGS We found two chromosomal groups differentiated by the number of X chromosomes and the chromosomal position of the ribosomal DNA clusters. In concordance, two main morphometric profiles were detected, clearly separating the Bolivian sample from the other ones. Phylogenetic DNA analyses showed that both chromosomal groups were closely related to each other and clearly separated from the remaining Panstrongylus species. High nucleotide divergence of cyt b and coI fragments were observed among P. rufotuberculatus samples from Bolivia, Colombia, Ecuador and Mexico (Kimura 2-parameter distances higher than 9%). MAIN CONCLUSIONS Chromosomal and molecular analyses supported that the two chromosomal groups could represent different closely related species. We propose that Bolivian individuals constitute a new Panstrongylus species, being necessary a detailed morphological study for its formal description. The clear morphometric discrimination based on the wing venation pattern suggests such morphological description might be conclusive.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/51314/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALCleberGalvao_SebastianPìta_etal_IOC_2021.pdfCleberGalvao_SebastianPìta_etal_IOC_2021.pdfapplication/pdf2758023https://www.arca.fiocruz.br/bitstream/icict/51314/2/CleberGalvao_SebastianP%c3%acta_etal_IOC_2021.pdfa19f870652dda841c0cd41ede16f222eMD52icict/513142023-09-05 10:15:30.393oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-09-05T13:15:30Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Multidisciplinary approach detects speciation within the kissing bug Panstrongylus rufotuberculatus populations (Hemiptera, Heteroptera, Reduviidae)
title Multidisciplinary approach detects speciation within the kissing bug Panstrongylus rufotuberculatus populations (Hemiptera, Heteroptera, Reduviidae)
spellingShingle Multidisciplinary approach detects speciation within the kissing bug Panstrongylus rufotuberculatus populations (Hemiptera, Heteroptera, Reduviidae)
Pita, Sebastián
Vetores da doença de Chagas
Gene do citocromo b
Subunidade I do citocromo C oxidase
Espaçador transcrito interno 2
Evolução do cariótipo
Análises morfométricas
Fusão dos cromossomos sexuais
Chagas disease vectors
Cytochrome b gene
Cytochrome C oxidase subunit I
Internal transcribed spacer 2
Karyotype evolution
Morphometric analyses
Sex chromosome fusion
title_short Multidisciplinary approach detects speciation within the kissing bug Panstrongylus rufotuberculatus populations (Hemiptera, Heteroptera, Reduviidae)
title_full Multidisciplinary approach detects speciation within the kissing bug Panstrongylus rufotuberculatus populations (Hemiptera, Heteroptera, Reduviidae)
title_fullStr Multidisciplinary approach detects speciation within the kissing bug Panstrongylus rufotuberculatus populations (Hemiptera, Heteroptera, Reduviidae)
title_full_unstemmed Multidisciplinary approach detects speciation within the kissing bug Panstrongylus rufotuberculatus populations (Hemiptera, Heteroptera, Reduviidae)
title_sort Multidisciplinary approach detects speciation within the kissing bug Panstrongylus rufotuberculatus populations (Hemiptera, Heteroptera, Reduviidae)
author Pita, Sebastián
author_facet Pita, Sebastián
Gómez-Palacio, Andrés
Lorite, Pedro
Dujardim, Jean Pierre
Chavez, Tamara
Villacís, Anita G.
Galvão, Cleber
Calleros, Lucía
Pereyra-Mello, Santiago
Burgueño-Rodríguez, Gabriela
Panzera, Francisco
author_role author
author2 Gómez-Palacio, Andrés
Lorite, Pedro
Dujardim, Jean Pierre
Chavez, Tamara
Villacís, Anita G.
Galvão, Cleber
Calleros, Lucía
Pereyra-Mello, Santiago
Burgueño-Rodríguez, Gabriela
Panzera, Francisco
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Pita, Sebastián
Gómez-Palacio, Andrés
Lorite, Pedro
Dujardim, Jean Pierre
Chavez, Tamara
Villacís, Anita G.
Galvão, Cleber
Calleros, Lucía
Pereyra-Mello, Santiago
Burgueño-Rodríguez, Gabriela
Panzera, Francisco
dc.subject.other.pt_BR.fl_str_mv Vetores da doença de Chagas
Gene do citocromo b
Subunidade I do citocromo C oxidase
Espaçador transcrito interno 2
Evolução do cariótipo
Análises morfométricas
Fusão dos cromossomos sexuais
topic Vetores da doença de Chagas
Gene do citocromo b
Subunidade I do citocromo C oxidase
Espaçador transcrito interno 2
Evolução do cariótipo
Análises morfométricas
Fusão dos cromossomos sexuais
Chagas disease vectors
Cytochrome b gene
Cytochrome C oxidase subunit I
Internal transcribed spacer 2
Karyotype evolution
Morphometric analyses
Sex chromosome fusion
dc.subject.en.pt_BR.fl_str_mv Chagas disease vectors
Cytochrome b gene
Cytochrome C oxidase subunit I
Internal transcribed spacer 2
Karyotype evolution
Morphometric analyses
Sex chromosome fusion
description Universidad de la República. Facultad de Ciencias. Sección Genética Evolutiva. Montevideo, Uruguay.
publishDate 2021
dc.date.issued.fl_str_mv 2021
dc.date.accessioned.fl_str_mv 2022-02-19T20:01:45Z
dc.date.available.fl_str_mv 2022-02-19T20:01:45Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv PITA, Sebastián et al. Multidisciplinary approach detects speciation within the kissing bug Panstrongylus rufotuberculatus populations (Hemiptera, Heteroptera, Reduviidae). Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 116, e210259, p. 1 - 12, 2021.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/51314
dc.identifier.issn.pt_BR.fl_str_mv 0074-0206
dc.identifier.doi.none.fl_str_mv 10.1590/0074-02760210259
identifier_str_mv PITA, Sebastián et al. Multidisciplinary approach detects speciation within the kissing bug Panstrongylus rufotuberculatus populations (Hemiptera, Heteroptera, Reduviidae). Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 116, e210259, p. 1 - 12, 2021.
0074-0206
10.1590/0074-02760210259
url https://www.arca.fiocruz.br/handle/icict/51314
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz.
publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz.
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
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collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/51314/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/51314/2/CleberGalvao_SebastianP%c3%acta_etal_IOC_2021.pdf
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