Gene expression profiling during early acute febrile stage of dengue infection can predict the disease outcome
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2009 |
| Outros Autores: | , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/26884 |
Resumo: | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Virologia e Terapia Experimental. Recife, PE, Brasil / The Johns Hopkins University School of Medicine. Division of Infectious Disease. Department of Medicine. Baltimore, Maryland, United States of America. |
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Nascimento, Eduardo J. M.Braga-Neto, UlissesCalzavara-Silva, Carlos E.Gomes, Ana L. V.Abath, Frederico Guilherme CoutinhoBrito, Carlos A. A.Cordeiro, Marli T.Silva, Ana M.Magalhães, CeciliaAndrade, RaoniGil, Laura H. V. G.Marques, Ernesto T. A.2018-06-14T11:53:13Z2018-06-14T11:53:13Z2009NASCIMENTO, E. J. M. et al. Gene expression profiling during early acute febrile stage of dengue infection can predict the disease outcome. PloS One, v. 4, n. 11, p. 1-16, 19 Nov. 2009.1932-6203https://www.arca.fiocruz.br/handle/icict/2688410.1371/journal.pone.0007892engDenguePCRDengue febril agudaDenguePCRAcute febrile dengueAdultoIdoso, 80 anos ou maisApoptoseBiomarcadores / metabolismoBrasilEstudos de coorteDengue / diagnósticoDengue / genéticaDengue / metabolismoVírus da Dengue / genéticaVírus da Dengue / metabolismoFêmeaFebre / diagnósticoFebre / metabolismoRegulamento de Expressão GênicaHumanosMasculinoMeia idadeAnálise multivariadaAnálise de Sequência de Sequência de OligonucleótidosResultado do tratamentoGene expression profiling during early acute febrile stage of dengue infection can predict the disease outcomeinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Virologia e Terapia Experimental. Recife, PE, Brasil / The Johns Hopkins University School of Medicine. Division of Infectious Disease. Department of Medicine. Baltimore, Maryland, United States of America.Texas A&M University. College Station. Department of Electrical Engineering. Texas, United States of America.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Virologia e Terapia Experimental. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Virologia e Terapia Experimental. Recife, PE, Brasil / Universidade Federal de Pernambuco. Departamento de Medicina Clínica. Recife, PE, Brazil / Hospital Esperança. Recife, PE, Brazil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Virologia e Terapia Experimental. Recife, PE, Brasil / Secretaria de Saúde do Estado de Pernambuco. Laboratório Central de Saúde Pública. Recife, PE, Brazil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Virologia e Terapia Experimental. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Virologia e Terapia Experimental. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Virologia e Terapia Experimental. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Virologia e Terapia Experimental. Recife, PE, Brasil.Background: We report the detailed development of biomarkers to predict the clinical outcome under dengue infection. Transcriptional signatures from purified peripheral blood mononuclear cells were derived from whole-genome geneexpression microarray data, validated by quantitative PCR and tested in independent samples. Methodology/Principal Findings: The study was performed on patients of a well-characterized dengue cohort from Recife, Brazil. The samples analyzed were collected prospectively from acute febrile dengue patients who evolved with different degrees of disease severity: classic dengue fever or dengue hemorrhagic fever (DHF) samples were compared with similar samples from other non-dengue febrile illnesses. The DHF samples were collected 2–3 days before the presentation of the plasma leakage symptoms. Differentially-expressed genes were selected by univariate statistical tests as well as multivariate classification techniques. The results showed that at early stages of dengue infection, the genes involved in effector mechanisms of innate immune response presented a weaker activation on patients who later developed hemorrhagic fever, whereas the genes involved in apoptosis were expressed in higher levels. Conclusions/Significance: Some of the gene expression signatures displayed estimated accuracy rates of more than 95%, indicating that expression profiling with these signatures may provide a useful means of DHF prognosis at early stages of infection.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83092https://www.arca.fiocruz.br/bitstream/icict/26884/1/license.txt447f2497af1ef5cf99b5c07f6fa18dceMD51ORIGINALGENE EXPRESSION PROFILING DURING.PDFGENE EXPRESSION PROFILING DURING.PDFapplication/pdf591321https://www.arca.fiocruz.br/bitstream/icict/26884/2/GENE%20EXPRESSION%20PROFILING%20DURING.PDF32135f71af62f4ce389581de8f5acf30MD52TEXTGENE EXPRESSION PROFILING DURING.PDF.txtGENE EXPRESSION PROFILING DURING.PDF.txtExtracted 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| dc.title.pt_BR.fl_str_mv |
Gene expression profiling during early acute febrile stage of dengue infection can predict the disease outcome |
| title |
Gene expression profiling during early acute febrile stage of dengue infection can predict the disease outcome |
| spellingShingle |
Gene expression profiling during early acute febrile stage of dengue infection can predict the disease outcome Nascimento, Eduardo J. M. Dengue PCR Dengue febril aguda Dengue PCR Acute febrile dengue Adulto Idoso, 80 anos ou mais Apoptose Biomarcadores / metabolismo Brasil Estudos de coorte Dengue / diagnóstico Dengue / genética Dengue / metabolismo Vírus da Dengue / genética Vírus da Dengue / metabolismo Fêmea Febre / diagnóstico Febre / metabolismo Regulamento de Expressão Gênica Humanos Masculino Meia idade Análise multivariada Análise de Sequência de Sequência de Oligonucleótidos Resultado do tratamento |
| title_short |
Gene expression profiling during early acute febrile stage of dengue infection can predict the disease outcome |
| title_full |
Gene expression profiling during early acute febrile stage of dengue infection can predict the disease outcome |
| title_fullStr |
Gene expression profiling during early acute febrile stage of dengue infection can predict the disease outcome |
| title_full_unstemmed |
Gene expression profiling during early acute febrile stage of dengue infection can predict the disease outcome |
| title_sort |
Gene expression profiling during early acute febrile stage of dengue infection can predict the disease outcome |
| author |
Nascimento, Eduardo J. M. |
| author_facet |
Nascimento, Eduardo J. M. Braga-Neto, Ulisses Calzavara-Silva, Carlos E. Gomes, Ana L. V. Abath, Frederico Guilherme Coutinho Brito, Carlos A. A. Cordeiro, Marli T. Silva, Ana M. Magalhães, Cecilia Andrade, Raoni Gil, Laura H. V. G. Marques, Ernesto T. A. |
| author_role |
author |
| author2 |
Braga-Neto, Ulisses Calzavara-Silva, Carlos E. Gomes, Ana L. V. Abath, Frederico Guilherme Coutinho Brito, Carlos A. A. Cordeiro, Marli T. Silva, Ana M. Magalhães, Cecilia Andrade, Raoni Gil, Laura H. V. G. Marques, Ernesto T. A. |
| author2_role |
author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Nascimento, Eduardo J. M. Braga-Neto, Ulisses Calzavara-Silva, Carlos E. Gomes, Ana L. V. Abath, Frederico Guilherme Coutinho Brito, Carlos A. A. Cordeiro, Marli T. Silva, Ana M. Magalhães, Cecilia Andrade, Raoni Gil, Laura H. V. G. Marques, Ernesto T. A. |
| dc.subject.other.pt_BR.fl_str_mv |
Dengue PCR Dengue febril aguda |
| topic |
Dengue PCR Dengue febril aguda Dengue PCR Acute febrile dengue Adulto Idoso, 80 anos ou mais Apoptose Biomarcadores / metabolismo Brasil Estudos de coorte Dengue / diagnóstico Dengue / genética Dengue / metabolismo Vírus da Dengue / genética Vírus da Dengue / metabolismo Fêmea Febre / diagnóstico Febre / metabolismo Regulamento de Expressão Gênica Humanos Masculino Meia idade Análise multivariada Análise de Sequência de Sequência de Oligonucleótidos Resultado do tratamento |
| dc.subject.en.pt_BR.fl_str_mv |
Dengue PCR Acute febrile dengue |
| dc.subject.decs.pt_BR.fl_str_mv |
Adulto Idoso, 80 anos ou mais Apoptose Biomarcadores / metabolismo Brasil Estudos de coorte Dengue / diagnóstico Dengue / genética Dengue / metabolismo Vírus da Dengue / genética Vírus da Dengue / metabolismo Fêmea Febre / diagnóstico Febre / metabolismo Regulamento de Expressão Gênica Humanos Masculino Meia idade Análise multivariada Análise de Sequência de Sequência de Oligonucleótidos Resultado do tratamento |
| description |
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Virologia e Terapia Experimental. Recife, PE, Brasil / The Johns Hopkins University School of Medicine. Division of Infectious Disease. Department of Medicine. Baltimore, Maryland, United States of America. |
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2009 |
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2009 |
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2018-06-14T11:53:13Z |
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2018-06-14T11:53:13Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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NASCIMENTO, E. J. M. et al. Gene expression profiling during early acute febrile stage of dengue infection can predict the disease outcome. PloS One, v. 4, n. 11, p. 1-16, 19 Nov. 2009. |
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https://www.arca.fiocruz.br/handle/icict/26884 |
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1932-6203 |
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10.1371/journal.pone.0007892 |
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NASCIMENTO, E. J. M. et al. Gene expression profiling during early acute febrile stage of dengue infection can predict the disease outcome. PloS One, v. 4, n. 11, p. 1-16, 19 Nov. 2009. 1932-6203 10.1371/journal.pone.0007892 |
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