Evaluation of resistance to pyrethroid and organophosphate adulticides and kdr genotyping in Aedes aegypti populations from Roraima, the northernmost Brazilian State

Detalhes bibliográficos
Autor(a) principal: Hayd, Ramão Luciano Nogueira
Data de Publicação: 2020
Outros Autores: Carrara, Luana, Lima, Joel de Melo, Almeida, Nathalia Coelho Vargas de, Lima, José Bento Pereira, Martins, Ademir Jesus
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/42396
Resumo: Universidade Federal de Roraima. Laboratório de Parasitologia e Monitoramento de Artrópodes Vetores da Amazônia. Boa Vista, RR, Brasil.
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spelling Hayd, Ramão Luciano NogueiraCarrara, LuanaLima, Joel de MeloAlmeida, Nathalia Coelho Vargas deLima, José Bento PereiraMartins, Ademir Jesus2020-07-25T18:44:05Z2020-07-25T18:44:05Z2020HAYD, Ramão Luciano Nogueira et al. Evaluation of resistance to pyrethroid and organophosphate adulticides and kdr genotyping in Aedes aegypti populations from Roraima, the northernmost Brazilian State. Parasite & Vectors, v. 13, n. 264, p. 1-9, 2020.1756-3305https://www.arca.fiocruz.br/handle/icict/4239610.1186/s13071-020-04127-wengBMCAedes aegyptiResistênciaAdulticídeos organofosfatosRoraimaPiretróideAedes aegyptiPyrethroidOrganophosphate adulticidesRoraimaNorthernmost Brazilian StateResistanceEvaluation of resistance to pyrethroid and organophosphate adulticides and kdr genotyping in Aedes aegypti populations from Roraima, the northernmost Brazilian Stateinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de Roraima. Laboratório de Parasitologia e Monitoramento de Artrópodes Vetores da Amazônia. Boa Vista, RR, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Fisiologia e Controle de Artrópodes Vetores. Rio de Janeiro, RJ, Brasil.Secretaria de Estado da Saúde de Roraima. Coordenadoria Geral de Vigilância em Saúde. Núcleo de Febre Amarela e Dengue. Boa Vista, RR, Brasil.Secretaria de Estado da Saúde de Roraima. Coordenadoria Geral de Vigilância em Saúde. Núcleo de Estadual de Entomologia. Boa Vista, RR, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Fisiologia e Controle de Artrópodes Vetores. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Fisiologia e Controle de Artrópodes Vetores. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular. Rio de Janeiro, RJ, Brasil.Background: Roraima, the northernmost State in Brazil, borders Venezuela and Guyana. Although mostly covered by the tropical forests, the urban centers of this state are highly infested with Ae. aegypti and are endemic for dengue, Zika and chikungunya. We accessed the insecticide resistance status of Ae. aegypti populations from the capital Boa Vista, two cities on international borders (Pacaraima and Bonfm) and Rorainópolis bordering Amazonas State, in order to evaluate the chemical control efcacy in these localities. Methods: Tests with World Health Organization (WHO)-like tubes impregnated with the pyrethroid deltamethrin (0.05% and 0.12%) and the organophosphate malathion (0.7%) were conducted with Ae. aegypti from Boa Vista, Pacaraima, Bonfm and Rorainópolis, collected in 2016 and 2018. Genotyping of kdr mutations, related to resistance to pyrethroids, was performed for the SNP variations at sites 1016 and 1534 of the voltage gated sodium channel gene (NaV) with a TaqMan qPCR approach. Results: Aedes albopictus was absent in our collections, and therefore only Ae. aegypti was tested. All Ae. aegypti populations were susceptible to 0.7% malathion in 2016; however, mortality dropped to under 90% in Bonfm and Pacaraima populations in 2018. All populations were resistant to 0.05% deltamethrin in both years. The time that 50% of females sufered knockdown (KdT50) under exposure to 0.05% deltamethrin was 3.3–5.9-fold longer in mosquitoes from the natural populations compared to the susceptible Rockefeller strain. Only the Pacaraima population (2018) remained resistant to 0.12% deltamethrin. Kdr genotyping revealed the absence of the wild-type NaVS haplotype (1016Val+1534Phe) in the populations from Roraima, indicating that all tested insects had a genetic background for pyrethroid resistance. The double kdr NaVR2 haplotype (1016Ile+15434Cys) was present in higher frequencies in all populations except for Rorainópolis, where this haplotype seems to have arrived recently. Conclusions: These results are important for the knowledge about insecticide resistance status of Ae. aegypti populations from Roraima and will help improve vector control strategies that may be applied to diverse localities under similar geographical and urban conditions.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/42396/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALLuanaCarrara_AdemirJMartins_etal_IOC_2020.pdfLuanaCarrara_AdemirJMartins_etal_IOC_2020.pdfapplication/pdf2038182https://www.arca.fiocruz.br/bitstream/icict/42396/2/LuanaCarrara_AdemirJMartins_etal_IOC_2020.pdfb2aa2999449cd3fc4003be9937c446e1MD52TEXTLuanaCarrara_AdemirJMartins_etal_IOC_2020.pdf.txtLuanaCarrara_AdemirJMartins_etal_IOC_2020.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Evaluation of resistance to pyrethroid and organophosphate adulticides and kdr genotyping in Aedes aegypti populations from Roraima, the northernmost Brazilian State
title Evaluation of resistance to pyrethroid and organophosphate adulticides and kdr genotyping in Aedes aegypti populations from Roraima, the northernmost Brazilian State
spellingShingle Evaluation of resistance to pyrethroid and organophosphate adulticides and kdr genotyping in Aedes aegypti populations from Roraima, the northernmost Brazilian State
Hayd, Ramão Luciano Nogueira
Aedes aegypti
Resistência
Adulticídeos organofosfatos
Roraima
Piretróide
Aedes aegypti
Pyrethroid
Organophosphate adulticides
Roraima
Northernmost Brazilian State
Resistance
title_short Evaluation of resistance to pyrethroid and organophosphate adulticides and kdr genotyping in Aedes aegypti populations from Roraima, the northernmost Brazilian State
title_full Evaluation of resistance to pyrethroid and organophosphate adulticides and kdr genotyping in Aedes aegypti populations from Roraima, the northernmost Brazilian State
title_fullStr Evaluation of resistance to pyrethroid and organophosphate adulticides and kdr genotyping in Aedes aegypti populations from Roraima, the northernmost Brazilian State
title_full_unstemmed Evaluation of resistance to pyrethroid and organophosphate adulticides and kdr genotyping in Aedes aegypti populations from Roraima, the northernmost Brazilian State
title_sort Evaluation of resistance to pyrethroid and organophosphate adulticides and kdr genotyping in Aedes aegypti populations from Roraima, the northernmost Brazilian State
author Hayd, Ramão Luciano Nogueira
author_facet Hayd, Ramão Luciano Nogueira
Carrara, Luana
Lima, Joel de Melo
Almeida, Nathalia Coelho Vargas de
Lima, José Bento Pereira
Martins, Ademir Jesus
author_role author
author2 Carrara, Luana
Lima, Joel de Melo
Almeida, Nathalia Coelho Vargas de
Lima, José Bento Pereira
Martins, Ademir Jesus
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Hayd, Ramão Luciano Nogueira
Carrara, Luana
Lima, Joel de Melo
Almeida, Nathalia Coelho Vargas de
Lima, José Bento Pereira
Martins, Ademir Jesus
dc.subject.other.pt_BR.fl_str_mv Aedes aegypti
Resistência
Adulticídeos organofosfatos
Roraima
Piretróide
topic Aedes aegypti
Resistência
Adulticídeos organofosfatos
Roraima
Piretróide
Aedes aegypti
Pyrethroid
Organophosphate adulticides
Roraima
Northernmost Brazilian State
Resistance
dc.subject.en.pt_BR.fl_str_mv Aedes aegypti
Pyrethroid
Organophosphate adulticides
Roraima
Northernmost Brazilian State
Resistance
description Universidade Federal de Roraima. Laboratório de Parasitologia e Monitoramento de Artrópodes Vetores da Amazônia. Boa Vista, RR, Brasil.
publishDate 2020
dc.date.accessioned.fl_str_mv 2020-07-25T18:44:05Z
dc.date.available.fl_str_mv 2020-07-25T18:44:05Z
dc.date.issued.fl_str_mv 2020
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv HAYD, Ramão Luciano Nogueira et al. Evaluation of resistance to pyrethroid and organophosphate adulticides and kdr genotyping in Aedes aegypti populations from Roraima, the northernmost Brazilian State. Parasite & Vectors, v. 13, n. 264, p. 1-9, 2020.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/42396
dc.identifier.issn.pt_BR.fl_str_mv 1756-3305
dc.identifier.doi.none.fl_str_mv 10.1186/s13071-020-04127-w
identifier_str_mv HAYD, Ramão Luciano Nogueira et al. Evaluation of resistance to pyrethroid and organophosphate adulticides and kdr genotyping in Aedes aegypti populations from Roraima, the northernmost Brazilian State. Parasite & Vectors, v. 13, n. 264, p. 1-9, 2020.
1756-3305
10.1186/s13071-020-04127-w
url https://www.arca.fiocruz.br/handle/icict/42396
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv BMC
publisher.none.fl_str_mv BMC
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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https://www.arca.fiocruz.br/bitstream/icict/42396/2/LuanaCarrara_AdemirJMartins_etal_IOC_2020.pdf
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