Avaliação da confiabilidade entre dois observadores em exames citopatológico e imunocitoquímico de aspirado de medula óssea no diagnóstico da leishmaniose visceral canina

Detalhes bibliográficos
Autor(a) principal: Vasconcelos, T. C. B.
Data de Publicação: 2016
Outros Autores: Machado, L. C., Abrantes, Tuanne Rotti, Menezes, R. C., Madeira, M. F., Ferreira, L. C., Figueiredo, F. B.
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/25587
Resumo: Universidade Federal Fluminense, Niterói, RJ, Brasil.
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spelling Vasconcelos, T. C. B.Machado, L. C.Abrantes, Tuanne RottiMenezes, R. C.Madeira, M. F.Ferreira, L. C.Figueiredo, F. B.2018-04-03T15:48:20Z2018-04-03T15:48:20Z2016VASCONCELOS, T. C. B. et al. Avaliação da confiabilidade entre dois observadores em exames citopatológico e imunocitoquímico de aspirado de medula óssea no diagnóstico da leishmaniose visceral canina. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, v. 68, n. 3, p. 821-824, 2016.0102-0935https://www.arca.fiocruz.br/handle/icict/2558710.1590/1678-4162-8762porAvaliação da confiabilidade entre dois observadores em exames citopatológico e imunocitoquímico de aspirado de medula óssea no diagnóstico da leishmaniose visceral caninaReliability evaluation between two evaluators in the cytopathologic and immunocytochemical exams from bone marrow aspirate in the canine visceral leishmaniasis diagnosisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal Fluminense, Niterói, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia. Rio de Janeiro, RJ, Brasil.Visceral leishmaniasis is a zoonosis in which the dog appears as the main source of infection in urban areas. Its diagnosis is complex and the cytopathological exam is a fast and cheap alternative to parasite direct visualization and its sensitivity can be increased by immunocytochemistry, though with a higher cost. The accuracy of such methods is dependent on the microscopist's experience and therefore, this study evaluated the reliability of such techniques between two observers, from bone marrow aspirates of 50 dogs from an endemic area for the disease. The parasitological culture in Novy-MacNeal-Nicolle medium was used as the reference standard. Among the main findings, the sensitivities obtained by observers I and II were respectively 62.5% and 37.5%, while specificities were 81.1% and 100%. On immunocytochemistry evaluation, the sensitivity was 0% for both evaluators and the specificity 97.3% and 100%. The agreement between evaluators was weak (κ = 0.167) for the cytopathological test and it could not be evaluated for immunocytochemistry, for which there was no detection by the evaluator II. The agreements among the diagnostic methods and the standard reference for the observer I were reasonable (κ = 0.364) for cytopathological examination and bad (κ = -0.041) for immunocytochemistry. For observer II, such agreement could be assessed only for the cytopathological test, being moderate (κ = 0.497). The results point to the possible expertise difference between evaluators, with the evaluator II demonstrating greater experience when interpreting the citopathological test. Although there was the expected sensitivity increase with immunocytochemistry, the technique used in this study was not effective for the diagnosis of infection, regardless of the evaluator.DogReliabilityLeishmania infantumDiagnosticinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83099https://www.arca.fiocruz.br/bitstream/icict/25587/1/license.txt586c046dcfeef936e32f0323bb9a47c0MD51ORIGINALve_Vasconcelos_etal_INI_2016.pdfve_Vasconcelos_etal_INI_2016.pdfapplication/pdf184571https://www.arca.fiocruz.br/bitstream/icict/25587/2/ve_Vasconcelos_etal_INI_2016.pdf6b8e1540ff46b21954def0a0824e2251MD52TEXTve_Vasconcelos_etal_INI_2016.pdf.txtve_Vasconcelos_etal_INI_2016.pdf.txtExtracted texttext/plain16870https://www.arca.fiocruz.br/bitstream/icict/25587/3/ve_Vasconcelos_etal_INI_2016.pdf.txtd765cc85c182f8e25f095aeabbff3d75MD53icict/255872022-03-23 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dc.title.pt_BR.fl_str_mv Avaliação da confiabilidade entre dois observadores em exames citopatológico e imunocitoquímico de aspirado de medula óssea no diagnóstico da leishmaniose visceral canina
dc.title.alternative.pt_BR.fl_str_mv Reliability evaluation between two evaluators in the cytopathologic and immunocytochemical exams from bone marrow aspirate in the canine visceral leishmaniasis diagnosis
title Avaliação da confiabilidade entre dois observadores em exames citopatológico e imunocitoquímico de aspirado de medula óssea no diagnóstico da leishmaniose visceral canina
spellingShingle Avaliação da confiabilidade entre dois observadores em exames citopatológico e imunocitoquímico de aspirado de medula óssea no diagnóstico da leishmaniose visceral canina
Vasconcelos, T. C. B.
Dog
Reliability
Leishmania infantum
Diagnostic
title_short Avaliação da confiabilidade entre dois observadores em exames citopatológico e imunocitoquímico de aspirado de medula óssea no diagnóstico da leishmaniose visceral canina
title_full Avaliação da confiabilidade entre dois observadores em exames citopatológico e imunocitoquímico de aspirado de medula óssea no diagnóstico da leishmaniose visceral canina
title_fullStr Avaliação da confiabilidade entre dois observadores em exames citopatológico e imunocitoquímico de aspirado de medula óssea no diagnóstico da leishmaniose visceral canina
title_full_unstemmed Avaliação da confiabilidade entre dois observadores em exames citopatológico e imunocitoquímico de aspirado de medula óssea no diagnóstico da leishmaniose visceral canina
title_sort Avaliação da confiabilidade entre dois observadores em exames citopatológico e imunocitoquímico de aspirado de medula óssea no diagnóstico da leishmaniose visceral canina
author Vasconcelos, T. C. B.
author_facet Vasconcelos, T. C. B.
Machado, L. C.
Abrantes, Tuanne Rotti
Menezes, R. C.
Madeira, M. F.
Ferreira, L. C.
Figueiredo, F. B.
author_role author
author2 Machado, L. C.
Abrantes, Tuanne Rotti
Menezes, R. C.
Madeira, M. F.
Ferreira, L. C.
Figueiredo, F. B.
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Vasconcelos, T. C. B.
Machado, L. C.
Abrantes, Tuanne Rotti
Menezes, R. C.
Madeira, M. F.
Ferreira, L. C.
Figueiredo, F. B.
dc.subject.en.pt_BR.fl_str_mv Dog
Reliability
Leishmania infantum
Diagnostic
topic Dog
Reliability
Leishmania infantum
Diagnostic
description Universidade Federal Fluminense, Niterói, RJ, Brasil.
publishDate 2016
dc.date.issued.fl_str_mv 2016
dc.date.accessioned.fl_str_mv 2018-04-03T15:48:20Z
dc.date.available.fl_str_mv 2018-04-03T15:48:20Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.citation.fl_str_mv VASCONCELOS, T. C. B. et al. Avaliação da confiabilidade entre dois observadores em exames citopatológico e imunocitoquímico de aspirado de medula óssea no diagnóstico da leishmaniose visceral canina. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, v. 68, n. 3, p. 821-824, 2016.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/25587
dc.identifier.issn.pt_BR.fl_str_mv 0102-0935
dc.identifier.doi.none.fl_str_mv 10.1590/1678-4162-8762
identifier_str_mv VASCONCELOS, T. C. B. et al. Avaliação da confiabilidade entre dois observadores em exames citopatológico e imunocitoquímico de aspirado de medula óssea no diagnóstico da leishmaniose visceral canina. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, v. 68, n. 3, p. 821-824, 2016.
0102-0935
10.1590/1678-4162-8762
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