Fluorescent membrane markers elucidate the association of Borrelia burgdorferi with tick cell lines

Detalhes bibliográficos
Autor(a) principal: Teixeira, R. C.
Data de Publicação: 2016
Outros Autores: Baêta, B. A., Ferreira, J. S., Medeiros, R. C., Monteiro, C. M. Maya, Lara, F. A., Bell-Sakyi, L., Fonseca, A. H.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/16516
Resumo: Universidade Federal Rural do Rio de Janeiro. Instituto de Veterinária. Laboratório de Doenças Parasitárias. Seropédica, RJ, Brasil.
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spelling Teixeira, R. C.Baêta, B. A.Ferreira, J. S.Medeiros, R. C.Monteiro, C. M. MayaLara, F. A.Bell-Sakyi, L.Fonseca, A. H.2016-12-13T12:12:03Z2016-12-13T12:12:03Z2016TEIXEIRA, R. C. et al. Fluorescent membrane markers elucidate the association of Borrelia burgdorferi with tick cell lines. Brazilian Journal of Medical and Biological Research, v.49, n.7, e5211, 9p, 2016.0100-879Xhttps://www.arca.fiocruz.br/handle/icict/1651610.1590/1414-431X201652111414-431XengAssociação Brasileira de Divulgação CientíficaBorrelia burgdorferiMarcador de membrana fluorescenteCarrapatosLinhas de célulasFagocitoseBorrelia burgdorferiTick cell linesPhagocytosisFluorescent membrane markerFluorescent membrane markers elucidate the association of Borrelia burgdorferi with tick cell linesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal Rural do Rio de Janeiro. Instituto de Veterinária. Laboratório de Doenças Parasitárias. Seropédica, RJ, Brasil.Universidade Federal Rural do Rio de Janeiro. Instituto de Veterinária. Laboratório de Doenças Parasitárias. Seropédica, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ. Brasil.The Tick Cell Biobank. The Pirbright Institute. Pirbright, UK.Universidade Federal Rural do Rio de Janeiro. Instituto de Veterinária. Laboratório de Doenças Parasitárias. Seropédica, RJ, Brasil.This study aimed to describe the association of Borrelia burgdorferi s.s. with ixodid tick cell lines by flow cytometry and fluorescence and confocal microscopy. Spirochetes were stained with a fluorescent membrane marker (PKH67 or PKH26), inoculated into 8 different tick cell lines and incubated at 30°C for 24 h. PKH efficiently stained B. burgdorferi without affecting bacterial viability or motility. Among the tick cell lines tested, the Rhipicephalus appendiculatus cell line RA243 achieved the highest percentage of association/internalization, with both high (90%) and low (10%) concentrations of BSK-H medium in tick cell culture medium. Treatment with cytochalasin D dramatically reduced the average percentage of cells with internalized spirochetes, which passed through a dramatic morphological change during their internalization by the host cell as observed in time-lapse photography. Almost all of the fluorescent bacteria were seen to be inside the tick cells. PKH labeling of borreliae proved to be a reliable and valuable tool to analyze the association of spirochetes with host cells by flow cytometry, confocal and fluorescence microscopy.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/16516/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALjs_ferreira_etal_IOC_2016.pdfjs_ferreira_etal_IOC_2016.pdfapplication/pdf3916655https://www.arca.fiocruz.br/bitstream/icict/16516/2/js_ferreira_etal_IOC_2016.pdfab3f6877057c3fa937250970f5dfe282MD52TEXTjs_ferreira_etal_IOC_2016.pdf.txtjs_ferreira_etal_IOC_2016.pdf.txtExtracted texttext/plain33380https://www.arca.fiocruz.br/bitstream/icict/16516/3/js_ferreira_etal_IOC_2016.pdf.txt9176cbcd6828561c9715cec3ef69c89bMD53icict/165162018-08-15 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dc.title.pt_BR.fl_str_mv Fluorescent membrane markers elucidate the association of Borrelia burgdorferi with tick cell lines
title Fluorescent membrane markers elucidate the association of Borrelia burgdorferi with tick cell lines
spellingShingle Fluorescent membrane markers elucidate the association of Borrelia burgdorferi with tick cell lines
Teixeira, R. C.
Borrelia burgdorferi
Marcador de membrana fluorescente
Carrapatos
Linhas de células
Fagocitose
Borrelia burgdorferi
Tick cell lines
Phagocytosis
Fluorescent membrane marker
title_short Fluorescent membrane markers elucidate the association of Borrelia burgdorferi with tick cell lines
title_full Fluorescent membrane markers elucidate the association of Borrelia burgdorferi with tick cell lines
title_fullStr Fluorescent membrane markers elucidate the association of Borrelia burgdorferi with tick cell lines
title_full_unstemmed Fluorescent membrane markers elucidate the association of Borrelia burgdorferi with tick cell lines
title_sort Fluorescent membrane markers elucidate the association of Borrelia burgdorferi with tick cell lines
author Teixeira, R. C.
author_facet Teixeira, R. C.
Baêta, B. A.
Ferreira, J. S.
Medeiros, R. C.
Monteiro, C. M. Maya
Lara, F. A.
Bell-Sakyi, L.
Fonseca, A. H.
author_role author
author2 Baêta, B. A.
Ferreira, J. S.
Medeiros, R. C.
Monteiro, C. M. Maya
Lara, F. A.
Bell-Sakyi, L.
Fonseca, A. H.
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Teixeira, R. C.
Baêta, B. A.
Ferreira, J. S.
Medeiros, R. C.
Monteiro, C. M. Maya
Lara, F. A.
Bell-Sakyi, L.
Fonseca, A. H.
dc.subject.other.pt_BR.fl_str_mv Borrelia burgdorferi
Marcador de membrana fluorescente
Carrapatos
Linhas de células
Fagocitose
topic Borrelia burgdorferi
Marcador de membrana fluorescente
Carrapatos
Linhas de células
Fagocitose
Borrelia burgdorferi
Tick cell lines
Phagocytosis
Fluorescent membrane marker
dc.subject.en.pt_BR.fl_str_mv Borrelia burgdorferi
Tick cell lines
Phagocytosis
Fluorescent membrane marker
description Universidade Federal Rural do Rio de Janeiro. Instituto de Veterinária. Laboratório de Doenças Parasitárias. Seropédica, RJ, Brasil.
publishDate 2016
dc.date.accessioned.fl_str_mv 2016-12-13T12:12:03Z
dc.date.available.fl_str_mv 2016-12-13T12:12:03Z
dc.date.issued.fl_str_mv 2016
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv TEIXEIRA, R. C. et al. Fluorescent membrane markers elucidate the association of Borrelia burgdorferi with tick cell lines. Brazilian Journal of Medical and Biological Research, v.49, n.7, e5211, 9p, 2016.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/16516
dc.identifier.issn.pt_BR.fl_str_mv 0100-879X
dc.identifier.doi.none.fl_str_mv 10.1590/1414-431X20165211
dc.identifier.eissn.none.fl_str_mv 1414-431X
identifier_str_mv TEIXEIRA, R. C. et al. Fluorescent membrane markers elucidate the association of Borrelia burgdorferi with tick cell lines. Brazilian Journal of Medical and Biological Research, v.49, n.7, e5211, 9p, 2016.
0100-879X
10.1590/1414-431X20165211
1414-431X
url https://www.arca.fiocruz.br/handle/icict/16516
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
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reponame_str Repositório Institucional da FIOCRUZ (ARCA)
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