Misguided phylogenetic comparisons using DGGE excised bands may contaminate public sequence databases

Detalhes bibliográficos
Autor(a) principal: Pylro, Victor Satler
Data de Publicação: 2016
Outros Autores: Morais, Daniel Kumazawa, Kalks, Karlos Henrique Martins, Roesch, Luiz Fernando Wurdig, Hirsch, Penny R., Tótola, Marcos Rogério, Yotoko, Karla
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/16269
Resumo: 2021-01-01
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spelling Pylro, Victor SatlerMorais, Daniel KumazawaKalks, Karlos Henrique MartinsRoesch, Luiz Fernando WurdigHirsch, Penny R.Tótola, Marcos RogérioYotoko, Karla2016-10-18T13:34:58Z2016-10-18T13:34:58Z2016PYLRO, Victor Satler et al. Misguided phylogenetic comparisons using DGGE excised bands may contaminate public sequence databases. Journal of Microbiological Methods, v. 126, p. 18-23, 2016.0167-7012https://www.arca.fiocruz.br/handle/icict/16269eng16S rRNA gene16S rRNA genePCR-DGGE bacteria phylogenyPublic databasesMisguided phylogenetic comparisons using DGGE excised bands may contaminate public sequence databasesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2021-01-01Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil.Universidade Federal de Viçosa. Departamento de Microbiologia. Viçosa, MG, Brasil.Universidade Federal do Pampa. São Gabriel, RS, Brasil.AgroEcology Department, Rothamsted Research, Harpenden, Herts, United Kingdom, UK.Universidade Federal de Viçosa. Departamento de Microbiologia. Viçosa, MG, Brasil.Universidade Federal de Viçosa. Departamento de Biologia. Viçosa, MG, Brasil.Controversy surrounding bacterial phylogenies has become one of the most important challenges for microbial ecology. Comparative analyses with nucleotide databases and phylogenetic reconstruction of the amplified 16S rRNA genes from DGGE (Denaturing Gradient Gel Electrophoresis) excised bands have been used by several researchers for the identification of organisms in complex samples. Here, we individually analyzed DGGE-excised 16S rRNA gene bands from 10 certified bacterial strains of different species, and demonstrated that this kind of approach can deliver erroneous outcomes to researchers, besides causing/emphasizing errors in public databases.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/16269/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALve_Pylro_Victor_Misguided_CPqRR_2016.pdfve_Pylro_Victor_Misguided_CPqRR_2016.pdfapplication/pdf413058https://www.arca.fiocruz.br/bitstream/icict/16269/2/ve_Pylro_Victor_Misguided_CPqRR_2016.pdf9ef3ae7d36b6db265afe718de38be1c1MD52TEXTve_Pylro_Victor_Misguided_CPqRR_2016.pdf.txtve_Pylro_Victor_Misguided_CPqRR_2016.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Misguided phylogenetic comparisons using DGGE excised bands may contaminate public sequence databases
title Misguided phylogenetic comparisons using DGGE excised bands may contaminate public sequence databases
spellingShingle Misguided phylogenetic comparisons using DGGE excised bands may contaminate public sequence databases
Pylro, Victor Satler
16S rRNA gene
16S rRNA gene
PCR-DGGE bacteria phylogeny
Public databases
title_short Misguided phylogenetic comparisons using DGGE excised bands may contaminate public sequence databases
title_full Misguided phylogenetic comparisons using DGGE excised bands may contaminate public sequence databases
title_fullStr Misguided phylogenetic comparisons using DGGE excised bands may contaminate public sequence databases
title_full_unstemmed Misguided phylogenetic comparisons using DGGE excised bands may contaminate public sequence databases
title_sort Misguided phylogenetic comparisons using DGGE excised bands may contaminate public sequence databases
author Pylro, Victor Satler
author_facet Pylro, Victor Satler
Morais, Daniel Kumazawa
Kalks, Karlos Henrique Martins
Roesch, Luiz Fernando Wurdig
Hirsch, Penny R.
Tótola, Marcos Rogério
Yotoko, Karla
author_role author
author2 Morais, Daniel Kumazawa
Kalks, Karlos Henrique Martins
Roesch, Luiz Fernando Wurdig
Hirsch, Penny R.
Tótola, Marcos Rogério
Yotoko, Karla
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Pylro, Victor Satler
Morais, Daniel Kumazawa
Kalks, Karlos Henrique Martins
Roesch, Luiz Fernando Wurdig
Hirsch, Penny R.
Tótola, Marcos Rogério
Yotoko, Karla
dc.subject.other.pt_BR.fl_str_mv 16S rRNA gene
topic 16S rRNA gene
16S rRNA gene
PCR-DGGE bacteria phylogeny
Public databases
dc.subject.en.pt_BR.fl_str_mv 16S rRNA gene
PCR-DGGE bacteria phylogeny
Public databases
description 2021-01-01
publishDate 2016
dc.date.accessioned.fl_str_mv 2016-10-18T13:34:58Z
dc.date.available.fl_str_mv 2016-10-18T13:34:58Z
dc.date.issued.fl_str_mv 2016
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv PYLRO, Victor Satler et al. Misguided phylogenetic comparisons using DGGE excised bands may contaminate public sequence databases. Journal of Microbiological Methods, v. 126, p. 18-23, 2016.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/16269
dc.identifier.issn.pt_BR.fl_str_mv 0167-7012
identifier_str_mv PYLRO, Victor Satler et al. Misguided phylogenetic comparisons using DGGE excised bands may contaminate public sequence databases. Journal of Microbiological Methods, v. 126, p. 18-23, 2016.
0167-7012
url https://www.arca.fiocruz.br/handle/icict/16269
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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instacron:FIOCRUZ
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instacron_str FIOCRUZ
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collection Repositório Institucional da FIOCRUZ (ARCA)
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