Biochemical characterization of a phospholipase A2 homologue from the venom of the social wasp Polybia occidentalis
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2018 |
| Outros Autores: | , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/35607 |
Resumo: | Fundação Oswaldo Cruz. Center for the Study of Biomolecules Applicable to Health. Porto Velho, RO, Brasil. / Federal University of Rondônia. Department of Medicine. Porto Velho, RO, Brasil. / Federal University of Rondônia. Postgraduate Program in Experimental Biology. Porto Velho, RO, Brasil. / São Lucas University Center. Porto Velho, RO, Brasil. |
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Sousa, Rafaela DinizKayano, Anderson MakotoCaldeira, Cleópatra Alves da SilvaSilva, Rodrigo SimõesMonteiro, Marta C.Dill, Leandro Soares MoreiraGrabner, Fernando P.Calderon, Leonardo de AzevedoZuliani, Juliana PavanStábeli, Rodrigo GuerinoSoares, Andreimar Martins2019-09-16T15:25:56Z2019-09-16T15:25:56Z2018SOUSA, Rafaela Diniz et al. Biochemical characterization of a phospholipase A2 homologue from the venom of the social wasp Polybia occidentalis. Journal of Venomous Animals and Toxins including Tropical Diseases, v. 24, p. 1-6, 2018.1678-9199https://www.arca.fiocruz.br/handle/icict/3560710.1186/s40409-018-0143-11678-9199engJournal of Venomous Animals and Toxins including Tropical DiseasesBiochemical characterization of a phospholipase A2 homologue from the venom of the social wasp Polybia occidentalisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Center for the Study of Biomolecules Applicable to Health. Porto Velho, RO, Brasil. / Federal University of Rondônia. Department of Medicine. Porto Velho, RO, Brasil. / Federal University of Rondônia. Postgraduate Program in Experimental Biology. Porto Velho, RO, Brasil. / São Lucas University Center. Porto Velho, RO, Brasil.Fundação Oswaldo Cruz. Center for the Study of Biomolecules Applicable to Health. Porto Velho, RO, Brasil. / Federal University of Rondônia. Department of Medicine. Porto Velho, RO, Brasil.Fundação Oswaldo Cruz. Center for the Study of Biomolecules Applicable to Health. Porto Velho, RO, Brasil. / Federal University of Rondônia. Department of Medicine. Porto Velho, RO, Brasil. / Federal University of Rondônia. Postgraduate Program in Biodiversity and Biotechnology. Porto Velho, RO, Brasil.Fundação Oswaldo Cruz. Center for the Study of Biomolecules Applicable to Health. Porto Velho, RO, Brasil. / Federal University of Rondônia. Department of Medicine. Porto Velho, RO, Brasil.Federal University of Pará. School of Pharmacy. Belém, PA, Brasil.Fundação Oswaldo Cruz. Center for the Study of Biomolecules Applicable to Health. Porto Velho, RO, Brasil. / Federal University of Rondônia. Department of Medicine. Porto Velho, RO, Brasil.Fundação Oswaldo Cruz. Center for the Study of Biomolecules Applicable to Health. Porto Velho, RO, Brasil. / São Lucas University Center. Porto Velho, RO, Brasil.Fundação Oswaldo Cruz. Center for the Study of Biomolecules Applicable to Health. Porto Velho, RO, Brasil. / Federal University of Rondônia. Department of Medicine. Porto Velho, RO, Brasil.Fundação Oswaldo Cruz. Center for the Study of Biomolecules Applicable to Health. Porto Velho, RO, Brasil. / Federal University of Rondônia. Department of Medicine. Porto Velho, RO, Brasil.Fundação Oswaldo Cruz. Center for the Study of Biomolecules Applicable to Health. Porto Velho, RO, Brasil. / Fundação Oswaldo Cruz. Universidade Federal de São Carlos. Department of Medicine. University of São Paulo. Center of Translational Medicine and School of Medicine of Ribeirão Preto. São Paulo, SP, Brasil.Fundação Oswaldo Cruz. Center for the Study of Biomolecules Applicable to Health. Porto Velho, RO, Brasil. / Federal University of Rondônia. Department of Medicine. Porto Velho, RO, Brasil. / São Lucas University Center. Porto Velho, RO, Brasil.Background: Wasp venoms constitute a molecular reservoir of new pharmacological substances such as peptides and proteins, biological property holders, many of which are yet to be identified. Exploring these sources may lead to the discovery of molecules hitherto unknown. This study describes, for the first time in hymenopteran venoms, the identification of an enzymatically inactive phospholipase A2 (PLA2) from the venom of the social wasp Polybia occidentalis. Methods: P. occidentalis venom was fractioned by molecular exclusion and reverse phase chromatography. For the biochemical characterization of the protein, 1D and 2D SDS-PAGE were performed, along with phospholipase activity assays on synthetic substrates, MALDI-TOF mass spectrometry and sequencing by Edman degradation. Results: The protein, called PocTX, was isolated using two chromatographic steps. Based on the phospholipase activity assay, electrophoresis and mass spectrometry, the protein presented a high degree of purity, with a mass of 13,896. 47 Da and a basic pI. After sequencing by the Edman degradation method, it was found that the protein showed a high identity with snake venom PLA2 homologues. Conclusion: This is the first report of an enzymatically inactive PLA2 isolated from wasp venom, similar to snake PLA2 homologues.WaspPolybia occidentalisPocTXPLA2 homologueinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/35607/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALBiochemical characterization of a phospholipase A2 homologue from the venom of the social wasp Polybia occidentalis.pdfBiochemical characterization of a phospholipase A2 homologue from the venom of the social wasp Polybia occidentalis.pdfapplication/pdf774140https://www.arca.fiocruz.br/bitstream/icict/35607/2/Biochemical%20characterization%20of%20a%20phospholipase%20A2%20homologue%20from%20the%20venom%20of%20the%20social%20wasp%20Polybia%20occidentalis.pdfa6084af74aef5a679e1e0acf5eae2de3MD52TEXTBiochemical characterization of a phospholipase A2 homologue from the venom of the social wasp Polybia occidentalis.pdf.txtBiochemical characterization of a phospholipase A2 homologue from the venom of the social wasp Polybia occidentalis.pdf.txtExtracted texttext/plain28653https://www.arca.fiocruz.br/bitstream/icict/35607/3/Biochemical%20characterization%20of%20a%20phospholipase%20A2%20homologue%20from%20the%20venom%20of%20the%20social%20wasp%20Polybia%20occidentalis.pdf.txt95bfdf21eaaafdf908b7afb411f29705MD53icict/356072019-09-18 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| dc.title.pt_BR.fl_str_mv |
Biochemical characterization of a phospholipase A2 homologue from the venom of the social wasp Polybia occidentalis |
| title |
Biochemical characterization of a phospholipase A2 homologue from the venom of the social wasp Polybia occidentalis |
| spellingShingle |
Biochemical characterization of a phospholipase A2 homologue from the venom of the social wasp Polybia occidentalis Sousa, Rafaela Diniz Wasp Polybia occidentalis PocTX PLA2 homologue |
| title_short |
Biochemical characterization of a phospholipase A2 homologue from the venom of the social wasp Polybia occidentalis |
| title_full |
Biochemical characterization of a phospholipase A2 homologue from the venom of the social wasp Polybia occidentalis |
| title_fullStr |
Biochemical characterization of a phospholipase A2 homologue from the venom of the social wasp Polybia occidentalis |
| title_full_unstemmed |
Biochemical characterization of a phospholipase A2 homologue from the venom of the social wasp Polybia occidentalis |
| title_sort |
Biochemical characterization of a phospholipase A2 homologue from the venom of the social wasp Polybia occidentalis |
| author |
Sousa, Rafaela Diniz |
| author_facet |
Sousa, Rafaela Diniz Kayano, Anderson Makoto Caldeira, Cleópatra Alves da Silva Silva, Rodrigo Simões Monteiro, Marta C. Dill, Leandro Soares Moreira Grabner, Fernando P. Calderon, Leonardo de Azevedo Zuliani, Juliana Pavan Stábeli, Rodrigo Guerino Soares, Andreimar Martins |
| author_role |
author |
| author2 |
Kayano, Anderson Makoto Caldeira, Cleópatra Alves da Silva Silva, Rodrigo Simões Monteiro, Marta C. Dill, Leandro Soares Moreira Grabner, Fernando P. Calderon, Leonardo de Azevedo Zuliani, Juliana Pavan Stábeli, Rodrigo Guerino Soares, Andreimar Martins |
| author2_role |
author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Sousa, Rafaela Diniz Kayano, Anderson Makoto Caldeira, Cleópatra Alves da Silva Silva, Rodrigo Simões Monteiro, Marta C. Dill, Leandro Soares Moreira Grabner, Fernando P. Calderon, Leonardo de Azevedo Zuliani, Juliana Pavan Stábeli, Rodrigo Guerino Soares, Andreimar Martins |
| dc.subject.en.pt_BR.fl_str_mv |
Wasp Polybia occidentalis PocTX PLA2 homologue |
| topic |
Wasp Polybia occidentalis PocTX PLA2 homologue |
| description |
Fundação Oswaldo Cruz. Center for the Study of Biomolecules Applicable to Health. Porto Velho, RO, Brasil. / Federal University of Rondônia. Department of Medicine. Porto Velho, RO, Brasil. / Federal University of Rondônia. Postgraduate Program in Experimental Biology. Porto Velho, RO, Brasil. / São Lucas University Center. Porto Velho, RO, Brasil. |
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2018 |
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2018 |
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2019-09-16T15:25:56Z |
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2019-09-16T15:25:56Z |
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SOUSA, Rafaela Diniz et al. Biochemical characterization of a phospholipase A2 homologue from the venom of the social wasp Polybia occidentalis. Journal of Venomous Animals and Toxins including Tropical Diseases, v. 24, p. 1-6, 2018. |
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https://www.arca.fiocruz.br/handle/icict/35607 |
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1678-9199 |
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10.1186/s40409-018-0143-1 |
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1678-9199 |
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SOUSA, Rafaela Diniz et al. Biochemical characterization of a phospholipase A2 homologue from the venom of the social wasp Polybia occidentalis. Journal of Venomous Animals and Toxins including Tropical Diseases, v. 24, p. 1-6, 2018. 1678-9199 10.1186/s40409-018-0143-1 |
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Journal of Venomous Animals and Toxins including Tropical Diseases |
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Journal of Venomous Animals and Toxins including Tropical Diseases |
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