Can interferon-gamma and interleukin-10 balance be associated with severity of human Leishmania (Viannia) braziliensis infection?

Detalhes bibliográficos
Autor(a) principal: Gomes-Silva, A.
Data de Publicação: 2007
Outros Autores: Bittar, R. de Cássia, Nogueira, R. dos Santos, Amato, V. S., Mattos, M. da Silva, Oliveira-Neto, M. P., Coutinho, S. G., Cruz, A. M. da
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/57016
Resumo: We are grateful to Ms Rosangela Pellegrino for secretarial assistance. This work was supported by Instituto Oswaldo Cruz/FIOCRUZ: internal funds; PAPES/VPPDT/FIOCRUZ, FAPERJ (grant number E-26/170·844/2003), FAPERJ/SUS: Ministério da Saúde and Conselho Nacional de Pesquisa e Desenvolvimento Tecnológico - CNPq (grant number E-26/ 170·614/2005). AMC is a research fellow of CNPq.
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spelling Gomes-Silva, A.Bittar, R. de CássiaNogueira, R. dos SantosAmato, V. S.Mattos, M. da SilvaOliveira-Neto, M. P.Coutinho, S. G.Cruz, A. M. da2023-02-11T01:19:59Z2023-02-11T01:19:59Z2007GOMES-SILVA, A. et al. Can interferon-gamma and interleukin-10 balance be associated with severity of human Leishmania (Viannia) braziliensis infection? Clinical and Experimental Immunology, v. 149, p. 440-444, 2007.0009-9104https://www.arca.fiocruz.br/handle/icict/5701610.1111/j.1365-2249.2007.03436.xWe are grateful to Ms Rosangela Pellegrino for secretarial assistance. This work was supported by Instituto Oswaldo Cruz/FIOCRUZ: internal funds; PAPES/VPPDT/FIOCRUZ, FAPERJ (grant number E-26/170·844/2003), FAPERJ/SUS: Ministério da Saúde and Conselho Nacional de Pesquisa e Desenvolvimento Tecnológico - CNPq (grant number E-26/ 170·614/2005). AMC is a research fellow of CNPq.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Imunologia. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Imunologia. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Imunologia. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil.Universidade de São Paulo. Faculdade de Medicina. Divisão Clínica de Moléstias Infecciosas e Parasitárias. Ambulatório de Leishmanioses. São Paulo, SP, Brasil.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Rio de Janeiro, RJ, Brasil.undação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Imunologia. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil.undação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Imunologia. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil.Suitable levels of interferon (IFN)-g and interleukin (IL)-10 seem to favour the outcome of cutaneous leishmaniasis (CL), while high IFN-g and low IL-10 production are associated with severity of mucosal leishmaniasis (ML). Considering that cytokine balance is important for the maintenance of protective responses in leishmaniasis, our aim was to investigate leishmanial antigensinduced IFN-g and IL-10 levels maintained in healed individuals who had different clinical outcomes of Leishmania infection. Thirty-three individuals who recovered from L. braziliensis infection were studied: cured CL (CCL), cured ML (CML), spontaneous healing of CL (SH) or asymptomatic individuals (ASY). Cytokines were quantified by enzyme-linked immunosorbent assay (ELISA) in culture supernatants of L. braziliensis-stimulated peripheral blood mononuclear cells (PBMC). IFN-g levels were higher in CML (7593 5994 pg/ml) in comparison to SH (3163 1526 pg/ml), ASY (1313 1048 pg/ml) or CCL (1897 2087 pg/ml). Moreover, cured ML cases maintained significantly lower production of IL-10 (127 57·8 pg/ml) in comparison to SH (1373 244 pg/ml), ASY (734 233 pg/ml) or CCL (542 375 pg/ml). Thus, a high IFN-g/IL-10 ratio observed in CML can indicate unfavourable cytokine balance. On the other hand, no significant difference in the IFN-g/IL-10 ratio was observed when CCL individuals were compared to SH or ASY subjects. In conclusion, even after clinical healing, ML patients maintained a high IFN-g/IL-10 secretion profile in response to leishmanial antigens. This finding can explain a delayed down-modulation of exacerbated inflammatory responses, which can be related in turn to the necessity of prolonged therapy in ML management. Conversely, lower IFN-g/IL-10 balance observed in CCL, SH and ASY individuals can represent a bettermodulated immune response associated with a favourable prognosis.engBritish Society for ImmunologyCan interferon-gamma and interleukin-10 balance be associated with severity of human Leishmania (Viannia) braziliensis infection?info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleClinical cureIFN-gIL-10Leishmania (Viannia) braziliensisRegulatory immune responsesinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALGomes-Silva_A_etal_INI_IOC_2007.pdfGomes-Silva_A_etal_INI_IOC_2007.pdfapplication/pdf161737https://www.arca.fiocruz.br/bitstream/icict/57016/2/Gomes-Silva_A_etal_INI_IOC_2007.pdfd403ee197dab80b293a49e42e44d35e6MD52LICENSElicense.txttext/plain1748https://www.arca.fiocruz.br/bitstream/icict/57016/1/license.txt8a4605be74aa9ea9d79846c1fba20a33MD51icict/570162023-09-04 11:30:21.654oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-09-04T14:30:21Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.en_US.fl_str_mv Can interferon-gamma and interleukin-10 balance be associated with severity of human Leishmania (Viannia) braziliensis infection?
title Can interferon-gamma and interleukin-10 balance be associated with severity of human Leishmania (Viannia) braziliensis infection?
spellingShingle Can interferon-gamma and interleukin-10 balance be associated with severity of human Leishmania (Viannia) braziliensis infection?
Gomes-Silva, A.
Clinical cure
IFN-g
IL-10
Leishmania (Viannia) braziliensis
Regulatory immune responses
title_short Can interferon-gamma and interleukin-10 balance be associated with severity of human Leishmania (Viannia) braziliensis infection?
title_full Can interferon-gamma and interleukin-10 balance be associated with severity of human Leishmania (Viannia) braziliensis infection?
title_fullStr Can interferon-gamma and interleukin-10 balance be associated with severity of human Leishmania (Viannia) braziliensis infection?
title_full_unstemmed Can interferon-gamma and interleukin-10 balance be associated with severity of human Leishmania (Viannia) braziliensis infection?
title_sort Can interferon-gamma and interleukin-10 balance be associated with severity of human Leishmania (Viannia) braziliensis infection?
author Gomes-Silva, A.
author_facet Gomes-Silva, A.
Bittar, R. de Cássia
Nogueira, R. dos Santos
Amato, V. S.
Mattos, M. da Silva
Oliveira-Neto, M. P.
Coutinho, S. G.
Cruz, A. M. da
author_role author
author2 Bittar, R. de Cássia
Nogueira, R. dos Santos
Amato, V. S.
Mattos, M. da Silva
Oliveira-Neto, M. P.
Coutinho, S. G.
Cruz, A. M. da
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Gomes-Silva, A.
Bittar, R. de Cássia
Nogueira, R. dos Santos
Amato, V. S.
Mattos, M. da Silva
Oliveira-Neto, M. P.
Coutinho, S. G.
Cruz, A. M. da
dc.subject.en.en_US.fl_str_mv Clinical cure
IFN-g
IL-10
Leishmania (Viannia) braziliensis
Regulatory immune responses
topic Clinical cure
IFN-g
IL-10
Leishmania (Viannia) braziliensis
Regulatory immune responses
description We are grateful to Ms Rosangela Pellegrino for secretarial assistance. This work was supported by Instituto Oswaldo Cruz/FIOCRUZ: internal funds; PAPES/VPPDT/FIOCRUZ, FAPERJ (grant number E-26/170·844/2003), FAPERJ/SUS: Ministério da Saúde and Conselho Nacional de Pesquisa e Desenvolvimento Tecnológico - CNPq (grant number E-26/ 170·614/2005). AMC is a research fellow of CNPq.
publishDate 2007
dc.date.issued.fl_str_mv 2007
dc.date.accessioned.fl_str_mv 2023-02-11T01:19:59Z
dc.date.available.fl_str_mv 2023-02-11T01:19:59Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv GOMES-SILVA, A. et al. Can interferon-gamma and interleukin-10 balance be associated with severity of human Leishmania (Viannia) braziliensis infection? Clinical and Experimental Immunology, v. 149, p. 440-444, 2007.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/57016
dc.identifier.issn.en_US.fl_str_mv 0009-9104
dc.identifier.doi.none.fl_str_mv 10.1111/j.1365-2249.2007.03436.x
identifier_str_mv GOMES-SILVA, A. et al. Can interferon-gamma and interleukin-10 balance be associated with severity of human Leishmania (Viannia) braziliensis infection? Clinical and Experimental Immunology, v. 149, p. 440-444, 2007.
0009-9104
10.1111/j.1365-2249.2007.03436.x
url https://www.arca.fiocruz.br/handle/icict/57016
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv British Society for Immunology
publisher.none.fl_str_mv British Society for Immunology
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
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reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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